Sébastien DEMENTIN

Chargé de Recherche, CNRS

+33 4 91 16 41 47

Equipe / Group: BIP 01

Je m’intéresse aux mécanismes mis en jeu lorsqu’un microorganisme doit s’adapter à changement de conditions de croissance ou à un stress. En particulier, en utilisant une approche génétique et biochimique, j’étudie le rôle des acteurs (chaperons, protéases, …) responsables du maintien de la protéostasie.
My studies aim at deciphering the processes involved in the adaptation of bacteria when growth conditions change or when they face stresses. In particular, using genetic and biochemical approaches, I study how chaperones and proteases control protein homeostasis when bacteria have to deal with adverse conditions.



Nathanael Jean Maillot, Pascale Infossi, Sébastien Dementin, Marie-Thérèse Giudici-Orticoni, Vincent Méjean, Olivier Genest.
Modulation of the RNA polymerase activity by AtcB, a protein associated with a DnaK chaperone network in Shewanella oneidensis.
Biochemical and Biophysical Research Communications, 2021, 535, 66 - 72. DOI: 10.1016/j.bbrc.2020.12.015


Elizabeth Charlotte Wittenborn, Chloé Guendon, Mériem Merrouch, Martino Benvenuti, Vincent Fourmond, Christophe Leger, Catherine L. Drennan, and Sébastien Dementin.
The solvent-exposed Fe-S D-cluster contributes to oxygen-resistance in D. vulgaris Ni-Fe carbon monoxide dehydrogenase.
ACS Cat., 2020, 10, 7328–7335. DOI: 10.1021/acscatal.0c00934 HAL: hal-02936282

Martino Benvenuti, Marta Meneghello, Chloé Guendon, Aurore Jacq-Bailly, Jae-hun Jeoung, Holger Dobbek, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
The two CO-dehydrogenases of Thermococcus sp. AM4.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 148188 -. DOI: 10.1016/j.bbabio.2020.148188 HAL: hal-02537797


Huaiguang Li, Darren Buesen, Sébastien Dementin, Christophe Léger, Vincent Fourmond, Nicolas Plumeré.
Complete Protection of O2-Sensitive Catalysts in Thin Films.
J. Am. Chem. Soc., 2019, 141 (42), 16734 - 16742. DOI: 10.1021/jacs.9b06790 HAL: hal-02369997v1

Elizabeth C. Wittenborn, Steven E. Cohen, Mériem Merrouch, Christophe Léger, Vincent Fourmond, Sébastien Dementin, Catherine L. Drennan.
Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase.
J. Biol. Chem., 2019, 257 (35), 6259 - 13026. DOI: 10.1074/jbc.RA119.009610 HAL: hal-02188452v1


Melisa del Barrio, Matteo Sensi, Christophe Orain, Carole Baffert, Sébastien Dementin, Vincent Fourmond, Christophe Léger.
Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels.
Acc. Chem. Res., 2018, 51 (3), 769 - 777. DOI: 10.1021/acs.accounts.7b00622 HAL: hal-01745738v1

Mériem Merrouch, Martino Benvenuti, Marco Lorenzi, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
Maturation of the [Ni–4Fe–4S] active site of carbon monoxide dehydrogenases.
J Biol Inorg Chem, 2018, 37 (4), 80 - 620. DOI: 10.1007/s00775-018-1541-0 HAL: hal-01816335v1

Elizabeth C Wittenborn, Mériem Merrouch, Chie Ueda, Laura Fradale, Christophe Léger, Vincent Fourmond, Maria-Eirini Pandelia, Sébastien Dementin, Catherine L Drennan.
Redox-dependent rearrangements of the NiFeS cluster of carbon monoxide dehydrogenase.
2018, 119, 13690 -. DOI: 10.7554/eLife.39451 HAL: hal-01917213v1


Lilith Domnik, Meriem Merrouch, Sebastian Goetzl, Jae-Hun Jeoung, Christophe Léger, Sébastien Dementin, Vincent Fourmond, Holger Dobbek.
CODH-IV: A High-Efficiency CO-Scavenging CO Dehydrogenase with Resistance to O2.
Angew. Chem. Int. Ed., 2017, 121 (48), 11034 - 15469. DOI: 10.1002/anie.201709261 HAL: hal-01696167v1

Meriem Merrouch, Jessica Hadj-Saïd, Christophe Léger, Sébastien Dementin, Vincent Fourmond.
Reliable estimation of the kinetic parameters of redox enzymes by taking into account mass transport towards rotating electrodes in protein film voltammetry experiments.
Electrochimica Acta, 2017, 245, 1059 - 1064. DOI: 10.1016/j.electacta.2017.03.114 HAL: hal-01614234v1


Pierre Ceccaldi, Emilien Etienne, Sébastien Dementin, Bruno Guigliarelli, Christophe Léger, Bénédicte Burlat.
Mechanism of inhibition of NiFe hydrogenase by nitric oxide.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (4), 454 - 461. DOI: 10.1016/j.bbabio.2016.01.014 HAL: hal-01413192v1


Abbas Abou-Hamdan, Pierre Ceccaldi, Hugo Lebrette, Oscar Gutiérrez-Sanz, Pierre Richaud, Laurent Cournac, Bruno Guigliarelli, Antonio L. De Lacey, Christophe Léger, Anne Volbeda, Bénédicte Burlat, Sébastien Dementin.
A Threonine Stabilizes the NiC and NiR Catalytic Intermediates of [NiFe]-hydrogenase.
J. Biol. Chem., 2015, 1837 (13), 131 - 8558. DOI: 10.1074/jbc.M114.630491 HAL: hal-01149507v1

Anne Volbeda, Lydie Martin, Elodie Barbier, Oscar Gutiérrez-Sanz, Antonio L. De Lacey, Pierre-Pol Liebgott, Sébastien Dementin, Marc Rousset, Juan C. Fontecilla-Camps.
Crystallographic studies of [NiFe]-hydrogenase mutants: towards consensus structures for the elusive unready oxidized states.
J Biol Inorg Chem, 2015, 355 (1), 473 - 22. DOI: 10.1007/s00775-014-1203-9 HAL: hal-01166131

Meriem Merrouch, Jessica Hadj-Saïd, Lilith Domnik, Holger Dobbek, Christophe Léger, Sébastien Dementin, Vincent Fourmond.
O2 Inhibition of Ni-Containing CO Dehydrogenase Is Partly Reversible.
Chem. Eur. J., 2015, 181 (52), 437 - 18938. DOI: 10.1002/chem.201502835 HAL: hal-01432205v1

Kajsa G.V. Sigfridsson, Nils Leidel, Oliver Sanganas, Petko Chernev, Oliver Lenz, Ki-Seok Yoon, Hirofumi Nishihara, Alison Parkin, Fraser A. Armstrong, Sébastien Dementin, Marc Rousset, Antonio L. De Lacey, Michael Haumann.
Structural differences of oxidized iron–sulfur and nickel–iron cofactors in O 2 -tolerant and O 2 -sensitive hydrogenases studied by X-ray absorption spectroscopy.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (2), 162 - 170. DOI: 10.1016/j.bbabio.2014.06.011 HAL: hal-01441931v1

Jessica Hadj-Saïd, Maria-Eirini Pandelia, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
The Carbon Monoxide Dehydrogenase from Desulfovibrio vulgaris.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (12), 1574 - 1583. DOI: 10.1016/j.bbabio.2015.08.002 HAL: hal-01430965v1


Claudio Greco, Vincent Fourmond, Carole Baffert, Po-hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger.
Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes.
Energy Environ. Sci., 2014, 108 (11), 2066 - 3573. DOI: 10.1039/C4EE01848F HAL: hal-01211585v1


Abbas Abou Hamdan, Bénédicte Burlat, Oscar Gutiérrez-Sanz, Pierre-Pol Liebgott, Carole Baffert, Antonio L De Lacey, Marc Rousset, Bruno Guigliarelli, Christophe Léger, Sébastien Dementin.
O2-independent formation of the inactive states of NiFe hydrogenase.
Nat Chem Biol, 2013, 118 (1), 12989 - 17. DOI: 10.1038/NCHEMBIO.1110 HAL: hal-01556905v1

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Thomas Lautier, Abbas Abou Hamdan, Sébastien Dementin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Christophe Léger.
Steady-State Catalytic Wave-Shapes for 2-Electron Reversible Electrocatalysts and Enzymes.
J. Am. Chem. Soc., 2013, 135 (10), 3926 - 3938. DOI: 10.1021/ja311607s HAL: hal-01268145v1

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Sébastien Dementin, Abbas Abou-Hamdan, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
The mechanism of inhibition by H2 of H2-evolution by hydrogenases.
Chem. Commun., 2013, 109 (61), 19916 -. DOI: 10.1039/C3CC43297A HAL: hal-01268212v1


A. Abou Hamdan, P.-P. Liebgott, V. Fourmond, O. Gutierrez-Sanz, A. L. De Lacey, P. Infossi, M. Rousset, S. Dementin, C. Leger.
Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants.
Proceedings of the National Academy of Sciences, 2012, 76 (1-2), 141 - 19921. DOI: 10.1073/pnas.1212258109 HAL: hal-01825482v1

Abbas Abou Hamdan, Sébastien Dementin, Pierre-Pol Liebgott, Oscar Gutierrez-Sanz, Pierre Richaud, Antonio L. De Lacey, Marc Rousset, Patrick Bertrand, Laurent Cournac, Christophe Léger.
Understanding and Tuning the Catalytic Bias of Hydrogenase.
J. Am. Chem. Soc., 2012, 134 (20), 8368 - 8371. DOI: 10.1021/ja301802r HAL: hal-00748324v1


Pierre-Pol Liebgott, Antonio L. de Lacey, Bénédicte Burlat, Laurent Cournac, Pierre Richaud, Myriam Brugna, Victor M. Fernandez, Bruno Guigliarelli, Marc Rousset, Christophe Léger, Sébastien Dementin.
Original Design of an Oxygen-Tolerant [NiFe] Hydrogenase: Major Effect of a Valine-to-Cysteine Mutation near the Active Site.
J. Am. Chem. Soc., 2011, 133 (4), 986 - 997. DOI: 10.1021/ja108787s HAL: hal-01977599v1

Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 - 10221. DOI: 10.1021/ja202615a HAL: hal-00677402v1

Pierre-Pol Liebgott, Sébastien Dementin, Christophe Léger, Marc Rousset.
Towards engineering O2-tolerance in [Ni–Fe] hydrogenases.
Energy Environ. Sci., 2011, 128 (1), 5209 - 41. DOI: 10.1039/c0ee00093k HAL: hal-01977604


Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Émilien Étienne, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants.
Biochemistry, 2010, 49 (11), 2424 - 2432. DOI: 10.1021/bi902140e HAL: hal-00677461

Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 275 (1), 113 - 70. DOI: 10.1038/nchembio.276 HAL: hal-01977629


Vincent Fourmond, Thomas Lautier, Carole Baffert, Fanny Leroux, Pierre-Pol Liebgott, Sébastien Dementin, Marc Rousset, Pascal Arnoux, David Pignol, Isabelle Meynial-Salles, Phillippe Soucaille, Patrick Bertrand, Christophe Léger.
Correcting for Electrocatalyst Desorption and Inactivation in Chronoamperometry Experiments.
Anal. Chem., 2009, 81 (8), 2962 - 2968. DOI: 10.1021/ac8025702 HAL: hal-01825488v1

Sébastien Dementin, Fanny Leroux, Laurent Cournac, Antonio L. de Lacey, Anne Volbeda, Christophe Léger, Bénédicte Burlat, Nicolas Martinez, Stéphanie Champ, Lydie Martin, Oliver Sanganas, Michael Haumann, Víctor M. Fernández, Bruno Guigliarelli, Ju.
Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant.
J. Am. Chem. Soc., 2009, 131 (29), 10156 - 10164. DOI: 10.1021/ja9018258


É. Lojou, X. Luo, M. Brugna, N. Candoni, S. Dementin, M. T. Giudici-Orticoni.
Biocatalysts for fuel cells: efficient hydrogenase orientation for H2 oxidation at electrodes modified with carbon nanotubes.
J Biol Inorg Chem, 2008, 13 (7), 1157 - 1167. DOI: 10.1007/s00775-008-0401-8 HAL: hal-00335503

F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset, C. Leger.
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences, 2008, 53 (Pt 3), 240 - 11193. DOI: 10.1073/pnas.0803689105 HAL: hal-00336010v1

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Pascal Arnoux, Monique Sabaty, Séverine Boiry, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Major Mo(V) EPR Signature of Rhodobacter sphaeroides Periplasmic Nitrate Reductase Arising from a Dead-End Species That Activates upon Reduction. Relation to Other Molybdoenzymes from the DMSO Reductase Family.
J. Phys. Chem. B, 2008, 112 (48), 15478 - 15486. DOI: 10.1021/jp807092y HAL: hal-00336002


Sébastien Dementin, Pascal Arnoux, Bettina Frangioni, Sandrine Grosse, Christophe Léger, Bénédicte Burlat, Bruno Guigliarelli, Monique Sabaty, David Pignol.
Access to the Active Site of Periplasmic Nitrate Reductase:  Insights from Site-Directed Mutagenesis and Zinc Inhibition Studies.
Biochemistry, 2007, 46 (34), 9713 - 9721. DOI: 10.1021/bi700928m HAL: hal-00336040

Patrick Bertrand, Bettina Frangioni, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Bruno Guigliarelli, David Pignol, Christophe Léger.
Effects of Slow Substrate Binding and Release in Redox Enzymes:  Theory and Application to Periplasmic Nitrate Reductase.
J. Phys. Chem. B, 2007, 111 (34), 10300 - 10311. DOI: 10.1021/jp074340j HAL: hal-00336030


Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 - 5218. DOI: 10.1021/ja060233b HAL: hal-00335157v1


Christophe Léger, Sébastien Dementin, Patrick Bertrand, Marc Rousset, Bruno Guigliarelli.
Inhibition and Aerobic Inactivation Kinetics of Desulfovibrio fructosovorans NiFe Hydrogenase Studied by Protein Film Voltammetry.
J. Am. Chem. Soc., 2004, 126 (38), 12162 - 12172. DOI: 10.1021/ja046548d HAL: hal-00336069