Alessio BONUCCI

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Post-doc, CNRS

Equipe / Group: BIP 07


I obtained my PhD in 2013 on Chemical science at University of Siena (Italy). My research was principally focused on the application of SDSL-EPR spectroscopy for the investigation of protein conformations and dynamics. I spent a period (2011-2012) as PhD visiting student in the EPR group of prof. W.L. Hubbell (UCLA, Los Angeles) to acquire advanced knowledge about the application of spin labels and EPR techniques in the field of structural biology. After my PhD (2014-2015) I had a postdoctoral position in the X-ray Crystallography group of prof. S. Mangani (University of Siena, Italy) working on the characterization of inhibitors of proteins implied in parasitic infections. Then, I joined the group of B. Guigliarelli (2015-2016, Marseille, France) to develop new strategies for in-cell EPR experiments on proteins. From 2017 to 2020, I was postdoc at CERM (Center Magnetic Resonance, University of Florence, Italy) under the supervision of Prof. R. Pierattelli and Prof. L. Banci to combine EPR and NMR spectroscopies for the structural analysis of different proteins in solution. In 2020, I moved back to the EPR group of CNRS-Marseille to work with V. Belle on the conformational analysis of proteins implied in DNA reparation mechanisms with SDSL-EPR and DEER approaches.
My research is focused on the application of paramagnetic tags for the characterization of structures and mechanisms of actions of proteins using multiple spectroscopic techniques, such as SDSL-EPR and NMR.


Publications
ORCID id: 0000-0002-4226-2864

2020

A. Bonucci, M. G. Murrali, L. Banci, R. Pierattelli.
A combined NMR and EPR investigation on the effect of the disordered RGG regions in the structure and the activity of the RRM domain of FUS.
Sci Rep, 2020, 10 (1). DOI: 10.1038/s41598-020-77899-x

Alessio Bonucci, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo.
In‐Cell EPR: Progress towards Structural Studies Inside Cells.
ChemBioChem, 2020, 21 (4), 451 - 460. DOI: 10.1002/cbic.201900291 HAL: hal-02180300v1



2019

Panagis Polykretis, Enrico Luchinat, Alessio Bonucci, Andrea Giachetti, Melissa A. Graewert, Dmitri I. Svergun, Lucia Banci.
Conformational characterization of full-length X-chromosome-linked inhibitor of apoptosis protein (XIAP) through an integrated approach.
Int Union Cryst J, 2019, 6 (5), 948 - 957. DOI: 10.1107/S205225251901073X

Pasquale Linciano, Cecilia Pozzi, Lucia dello Iacono, Flavio di Pisa, Giacomo Landi, Alessio Bonucci, Sheraz Gul, Maria Kuzikov, Bernhard Ellinger, Gesa Witt, Nuno Santarem, Catarina Baptista, Caio Franco, Carolina B. Moraes, Wolfgang Müller, Ulrike Wittig, Rosaria Luciani, Antony Sesenna, Antonio Quotadamo, Stefania Ferrari, Ina Pöhner, Anabela Cordeiro-da-Silva, Stefano Mangani, Luca Costantino, Maria Paola Costi.
Enhancement of Benzothiazoles as Pteridine Reductase-1 Inhibitors for the Treatment of Trypanosomatidic Infections.
J. Med. Chem., 2019, 62 (8), 3989 - 4012. DOI: 10.1021/acs.jmedchem.8b02021



2018

Ganesan Karthikeyan, Alessio Bonucci, Gilles Casano, Guillaume Gerbaud, Sébastien Abel, Virginie Thomé, Laurent Kodjabachian, Axel Magalon, Bruno Guigliarelli, Valérie Belle, Olivier Ouari, Elisabetta Mileo.
A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies.
Angew. Chem. Int. Ed., 2018, 57, 1366 - 1370. DOI: 10.1002/anie.201710184 HAL: hal-02000559v1



2017

Joanna Panecka-Hofman, Ina Pöhner, Francesca Spyrakis, Talia Zeppelin, Flavio Di Pisa, Lucia Dello Iacono, Alessio Bonucci, Antonio Quotadamo, Alberto Venturelli, Stefano Mangani, Maria Paola Costi, Rebecca C. Wade.
Comparative mapping of on-targets and off-targets for the discovery of anti-trypanosomatid folate pathway inhibitors.
Biochimica et Biophysica Acta (BBA) - General Subjects, 2017, 1861 (12), 3215 - 3230. DOI: 10.1016/j.bbagen.2017.09.012 HAL: hal-01809565v1

Marta Palombo, Alessio Bonucci, Emilien Etienne, Stefano Ciurli, Vladimir N. Uversky, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo, Barbara Zambelli.
The relationship between folding and activity in UreG, an intrinsically disordered enzyme.
Sci Rep, 2017, 89 (1), 392 -. DOI: 10.1038/s41598-017-06330-9 HAL: hal-01585289v1