Marianne GUIRAL

member photo

Chargée de Recherche, CNRS

+33 4 91 16 44 04

Equipe / Group: BIP 01


-PhD in Microbiology at the University of Provence (Marseille, France) in 2000
-Researcher (CRCN) at the Bioenergetics and Protein Engineering lab since 2003
-Skills:
Microbiology, Biochemistry, Protein purification, Blue-Native gel, Enzymology, Energy metabolism, Dissimilatory sulfur oxidation, Sulfur enzymes, Oxygen reduction, Hydrogenases
-Recent research interests:
Dissimilatory sulfur metabolism and carbon fixation in the model organism Aquifex aeolicus, a sulfur and hydrogen-oxidizing microaerophilic and autotrophic hyperthermophilic bacterium.

-Doctorat en Microbiologie à l’Université de Provence (Marseille, France) en 2000.
-Chargée de recherche au Laboratoire de Bioénergétique et Ingénierie des Protéines depuis 2003.
-Compétences :
Microbiologie, Biochimie des protéines, Purification de protéines, Gels Bleu Natifs, Enzymologie, Métabolisme énergétique, Oxydation du soufre dissimilatrice, Enzymes du soufre, Réduction de l’oxygène, Hydrogénases.
-Recherche récentes :
Métabolisme d’oxydation du soufre et fixation du carbone chez l’organisme modèle Aquifex aeolicus, bactérie microaérophile et hyperthermophile autotrophe oxydant l’hydrogène et les composés soufrés.


Publications
ORCID id: 0000-0002-6029-0914

2024

Giulia D'Ermo, Stéphane Audebert, Luc Camoin, Britta Planer‐Friedrich, Corinne Casiot‐Marouani, Sophie Delpoux, Régine Lebrun, Marianne Guiral, Barbara Schoepp‐Cothenet.
Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila.
Environmental Microbiology, 2024, 26 (6), - . DOI: 10.1111/1462-2920.16655 HAL: hal-04620414

Giulia D’Ermo, Marianne Guiral, Barbara Schoepp-Cothenet.
The complex interplay of sulfur and arsenic bioenergetic metabolisms in the arsenic geochemical cycle.
Geomicrobiology, Geomicrobiology: Natural and anthropogenic settings (Lucian C. Staicu Larry L. Barton Eds), 2024 (ISBN:Hardcover: 978-3-031-54305-0; Ebook: 978-3-031-54306-7 ). HAL: hal-04410384



2023

Laura Prioretti, Giulia D'Ermo, Pascale Infossi, Arlette Kpebe, Régine Lebrun, Marielle Bauzan, Elisabeth Lojou, Bruno Guigliarelli, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
Carbon Fixation in the Chemolithoautotrophic Bacterium Aquifex aeolicus Involves Two Low-Potential Ferredoxins as Partners of the PFOR and OGOR Enzymes.
Life, 2023, 13 (3), 627. DOI: 10.3390/life13030627 HAL: hal-04071170v1

Daniele Di Bari, Stepan Timr, Marianne Guiral, Marie-Thérèse Giudici-Orticoni, Tilo Seydel, Christian Beck, Caterina Petrillo, Philippe Derreumaux, Simone Melchionna, Fabio Sterpone, Judith Peters, Alessandro Paciaroni.
Diffusive Dynamics of Bacterial Proteome as a Proxy of Cell Death.
ACS Cent. Sci., 2023. DOI: 10.1021/acscentsci.2c01078 HAL: hal-03936222v1



2022

Laura Prioretti, Pascale Infossi, Arlette Kpebe, Giulia D’Ermo, Régine Lebrun, Elisabeth Lojou, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
Carbon fixation in the chemoautotrophic bacterium Aquifex aeolicus: the five-subunit PFOR and OGOR and two low-potential ferredoxins.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, 148811. DOI: 10.1016/j.bbabio.2022.148811



2021

David Ranava, Cassandra Backes, Ganesan Karthikeyan, Olivier Ouari, Audrey Soric, Marianne Guiral, María Luz Cárdenas, Marie Thérèse Giudici-Orticoni.
Metabolic Exchange and Energetic Coupling between Nutritionally Stressed Bacterial Species: Role of Quorum-Sensing Molecules.
mBio, 2021, 12 (1). DOI: 10.1128/mBio.02758-20 HAL: hal-03115469v1



2020

Marianne Guiral, Marie-Thérèse Giudici-Orticoni.
Microbe Profile: Aquifex aeolicus: an extreme heat-loving bacterium that feeds on gases and inorganic chemicals.
2020. DOI: 10.1099/mic.0.001010 HAL: hal-03102751v1

Souhela Boughanemi, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Barbara Schoepp-Cothenet, Marianne Guiral.
Sulfite oxidation by the quinone-reducing molybdenum sulfite dehydrogenase SoeABC from the bacterium Aquifex aeolicus.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 1861 (11), 148279 -. DOI: 10.1016/j.bbabio.2020.148279 HAL: hal-02936602v1



2018

Frauke Baymann, Barbara Schoepp-Cothenet, Simon Duval, Marianne Guiral, Myriam Brugna, Carole Baffert, Michael J. Russell, Wolfgang Nitschke.
On the Natural History of Flavin-Based Electron Bifurcation.
Front. Microbiol., 2018, 8, 357 -. DOI: 10.3389/fmicb.2018.01357 HAL: hal-01828959v1

M. Guiral, C. Neitzel, M. Salvador Castell, N. Martinez, M. T. Giudici-Orticoni, J. Peters.
The effect of pH on the dynamics of natural membranes.
Eur. Phys. J. E, 2018, 41 (2). DOI: 10.1140/epje/i2018-11630-y HAL: hal-01844806v1



2016

Frédéric Harb, Marie-Thérèse Giudici-Orticoni, Marianne Guiral, Bernard Tinland.
Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers.
Eur. Phys. J. E, 2016, 1666 (12), 105 -. DOI: 10.1140/epje/i2016-16127-1 HAL: hal-01435354v1

Souhela Boughanemi, Jordan Lyonnet, Pascale Infossi, Marielle Bauzan, Artémis Kosta, Sabrina Lignon, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
Microbial oxidative sulfur metabolism: biochemical evidence of the membrane-bound heterodisulfide reductase-like complex of the bacterium Aquifex aeolicus.
FEMS Microbiology Letters, 2016, 66 (15), 88 -. DOI: 10.1093/femsle/fnw156 HAL: hal-01436272v1



2015

Frédéric Harb, Laurence Prunetti, Marie-Thérèse Giudici-Orticoni, Marianne Guiral, Bernard Tinland.
Insertion and self-diffusion of a monotopic protein, the Aquifex aeolicus sulfide quinone reductase, in supported lipid bilayers.
Eur. Phys. J. E, 2015, 67 (10), 5179 -. DOI: 10.1140/epje/i2015-15110-8 HAL: hal-01435350v1

Cindy J. Castelle, Magali Roger, Marielle Bauzan, Myriam Brugna, Sabrina Lignon, Manfred Nimtz, Olga V. Golyshina, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
The aerobic respiratory chain of the acidophilic archaeon Ferroplasma acidiphilum : A membrane-bound complex oxidizing ferrous iron.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (8), 717 - 728. DOI: 10.1016/j.bbabio.2015.04.006 HAL: hal-01429900v1



2014

M. Roger, A. de Poulpiquet, A. Ciaccafava, M. Ilbert, M. Guiral, M. T. Giudici-Orticoni, E. Lojou.
Reconstitution of supramolecular organization involved in energy metabolism at electrochemical interfaces for biosensing and bioenergy production.
Anal Bioanal Chem, 2014, 406 (6), 1011 - 1027. DOI: 10.1007/s00216-013-7465-1 HAL: hal-01493475v1



2013

Anne De Poulpiquet, Alexandre Ciaccafava, Katarzyna Szot, Baptiste Pillain, Pascale Infossi, Marianne Guiral, Marcin Opallo, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Exploring Properties of a Hyperthermophilic Membrane-Bound Hydrogenase at Carbon Nanotube Modified Electrodes for a Powerful H 2 /O 2 Biofuel Cell.
Electroanalysis, 2013, 284 (3), 465 - 695. DOI: 10.1002/elan.201200405 HAL: hal-03079262



2012

Magali Roger, Cindy Castelle, Marianne Guiral, Pascale Infossi, Elisabeth Lojou, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Mineral respiration under extreme acidic conditions: from a supramolecular organization to a molecular adaptation in Acidithiobacillus ferrooxidans.
2012, 40 (6), 1324 - 1329. DOI: 10.1042/BST20120141

Clément Aussignargues, Marie-Cécile Giuliani, Pascale Infossi, Elisabeth Lojou, Marianne Guiral, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Rhodanese Functions as Sulfur Supplier for Key Enzymes in Sulfur Energy Metabolism.
J. Biol. Chem., 2012, 287 (24), 19936 - 19948. DOI: 10.1074/jbc.M111.324863

Marianne Guiral, Laurence Prunetti, Clément Aussignargues, Alexandre Ciaccafava, Pascale Infossi, Marianne Ilbert, Elisabeth Lojou, Marie-Thérèse Giudici-Orticoni.
The Hyperthermophilic Bacterium Aquifex aeolicus: From Respiratory Pathways to Extremely Resistant Enzymes and Biotechnological Applications.
2012, 61, 125 - 194. DOI: 10.1016/B978-0-12-394423-8.00004-4

Alexandre Ciaccafava, Pascale Infossi, Marianne Ilbert, Marianne Guiral, Sophie Lecomte, Marie Thérèse Giudici-Orticoni, Elisabeth Lojou.
Electrochemistry, AFM, and PM-IRRA Spectroscopy of Immobilized Hydrogenase: Role of a Hydrophobic Helix in Enzyme Orientation for Efficient H2 Oxidation.
Angew. Chem. Int. Ed., 2012, 51 (4), 953 - 956. DOI: 10.1002/anie.201107053



2010

Laurence Prunetti, Pascale Infossi, Myriam Brugna, Christine Ebel, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
New Functional Sulfide Oxidase-Oxygen Reductase Supercomplex in the Membrane of the Hyperthermophilic Bacterium Aquifex aeolicus.
J. Biol. Chem., 2010, 12 (53), 205 - 41826. DOI: 10.1074/jbc.M110.167841



2008

Cindy Castelle, Marianne Guiral, Guillaume Malarte, Fouzia Ledgham, Gisèle Leroy, Myriam Brugna, Marie-Thérèse Giudici-Orticoni.
A New Iron-oxidizing/O 2 -reducing Supercomplex Spanning Both Inner and Outer Membranes, Isolated from the Extreme Acidophile Acidithiobacillus ferrooxidans.
J. Biol. Chem., 2008, 283 (38), 25803 - 25811. DOI: 10.1074/jbc.M802496200



2006

M GUIRAL, P TRON, V BELLE, C AUBERT, C LEGER, B GUIGLIARELLI, M GIUDICIORTICONI.
Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties.
International Journal of Hydrogen Energy, 2006, 31 (11), 1424 - 1431. DOI: 10.1016/j.ijhydene.2006.06.007 HAL: hal-00335435



2005

Marianne Guiral, Gisèle Leroy, Pierre Bianco, Philippe Gallice, Bruno Guigliarelli, Mireille Bruschi, Wolfgang Nitschke, Marie-Thérèse Giudici-Orticoni.
Interaction and electron transfer between the high molecular weight cytochrome and cytochrome c3 from Desulfovibrio vulgaris Hildenborough: Kinetic, microcalorimetric, EPR and electrochemical studies.
Biochimica et Biophysica Acta (BBA) - General Subjects, 2005, 1723 (1-3), 45 - 54. DOI: 10.1016/j.bbagen.2005.01.015 HAL: hal-00339978v1