member photo

Maître de Conférence, HDR, Aix-Marseille Université

+33 4 91 16 45 57

Equipe / Group: BIP 07

Since many years, my research projects deal with the study of structural transitions in proteins, including Intrinsically Disordered Proteins (IDPs), flexible proteins, metalloproteins and membrane proteins. The approach I use is Site Directed Spin Labeling (SDSL), based on the use of a spin probe, and EPR spectroscopy (Electronic Paramagnetic Resonance). Spin probes can be either nitroxide molecules or metal centers (endogenous or incorporated). Distance measurements between spin probes is also an approach I use to follow structural modifications in biological systems. I am also interested to study the link of protein conformational changes to their functions, in particular electron transfer, and in original biological approaches using non natural amino acids combined with SDSL, using non commercial spin labels.

ORCID id: 0000-0001-8576-5675


Sybille Tachon, Eugénie Fournier, Christophe Decroos, Pascal Mansuelle, Emilien Etienne, Marc Maresca, Marlène Martinho, Valérie Belle, Thierry Tron, Ariane Jalila Simaan.
Chemical Modification of 1-Aminocyclopropane Carboxylic Acid (ACC) Oxidase: Cysteine Mutational Analysis, Characterization, and Bioconjugation with a Nitroxide Spin Label.
Mol Biotechnol, 2019, 61, 650 - 662. DOI: 10.1007/s12033-019-00191-5 HAL: hal-02157289v1

Marlène Martinho, Eugénie Fournier, Nolwenn Le Breton, Elisabetta Mileo, Valérie Belle.
Nitroxide spin labels: fabulous spy spins for biostructural EPR applications.
Electron Paramagnetic Resonance, 2019, 26, 66 - 88. DOI: 10.1039/9781788013888-00066

Nolwenn Le Breton, Sonia Longhi, Antal Rockenbauer, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle, Marlène Martinho.
Probing the dynamic properties of two sites simultaneously in a protein–protein interaction process: a SDSL-EPR study.
Phys. Chem. Chem. Phys., 2019, 21, 22584 - 22588. DOI: 10.1039/c9cp04660g HAL: hal-02341622v1

Pascale Barbier, Orgeta Zejneli, Marlène Martinho, Alessia Lasorsa, Valérie Belle, Caroline Smet-Nocca, Philipp O. Tsvetkov, François Devred, Isabelle Landrieu.
Role of Tau as a Microtubule-Associated Protein: Structural and Functional Aspects.
Front. Aging Neurosci., 2019, 11:204, 1 - 14. DOI: 10.3389/fnagi.2019.00204 HAL: hal-02279660v1

Eugénie Fournier, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Valérie Belle, Marlène Martinho.
Spin labels: spies in the heart of proteins.
Actualité Chimique, 2019, 443.

Eugénie Fournier, Sybille Tachon, Nicholas J. Fowler, Guillaume Gerbaud, Pascal Mansuelle, Pierre Dorlet, Sam P. De Visser, Valérie Belle, A. Jalila Simaan, Marlène Martinho.
The Hunt for the Closed Conformation of the Fruit‐Ripening Enzyme 1‐Aminocyclopropane‐1‐carboxylic Oxidase: A Combined Electron Paramagnetic Resonance and Molecular Dynamics Study.
Chem. Eur. J., 2019, 25 (60), 13766-13776. DOI: 10.1002/chem.201903003 HAL: hal-02303326v1


Pascal Griesmar, Marlène Martinho, Bruno Faure.
Synthèse de complexes du vanadium et leur analyse par spectroscopie d'absorption UV–visible et infra-rouge.
Bulletin de l'Union des Physiciens, 01/06/2018, 2018, 6, 1005.

Marlène Martinho, Diane Allegro, Isabelle Huvent, Charlotte Chabaud, Emilien Etienne, Hervé Kovacic, Bruno Guigliarelli, Vincent Peyrot, Isabelle Landrieu, Valérie Belle, Pascale Barbier.
Two Tau binding sites on tubulin revealed by thiol-disulfide exchanges.
Sci Rep, 2018, 8:13846, 1 - 11. DOI: 10.1038/s41598-018-32096-9 HAL: hal-01883844v1


Nadia El Bakkali-Tahéri, Sybille Tachon, Maylis Orio, Sylvain Bertaina, Marlène Martinho, Viviane Robert, Marius Réglier, Thierry Tron, Pierre Dorlet, A. Jalila Simaan.
Characterization of Cu(II)-reconstituted ACC Oxidase using experimental and theoretical approaches.
Archives of Biochemistry and Biophysics, 2017, 623-624, 31-41. DOI: 10.1016/ HAL: hal-01627933v1

Jennifer O. Bigelow, Jason England, Johannes E. M. N. Klein, Erik R. Farquhar, Jonathan R. Frisch, Marlène Martinho, Debasish Mandal, Eckard Münck, Sason Shaik, Lawrence Que.
Oxoiron(IV) Tetramethylcyclam Complexes with Axial Carboxylate Ligands: Effect of Tethering the Carboxylate on Reactivity.
Inorg. Chem., 2017, 56 (6), 3287 - 3301. DOI: 10.1021/acs.inorgchem.6b02659 HAL: hal-01514906v1

Emilien Etienne, Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Valérie Belle.
SimLabel: a graphical user interface to simulate continuous wave EPR spectra from site-directed spin labeling experiments.
Magn. Reson. Chem., 2017, 55 (8), 714 - 719. DOI: 10.1002/mrc.4578 HAL: hal-01444172v1


Nolwenn Le Breton, Tiona Adrianaivomananjaona, Guillaume Gerbaud, Emilien Etienne, Elena Bisetto, Alain Dautant, Bruno Guigliarelli, Francis Haraux, Marlène Martinho, Valérie Belle.
Dimerization interface and dynamic properties of yeast IF1 revealed by Site-Directed Spin Labeling EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (1), 89 - 97. DOI: 10.1016/j.bbabio.2015.10.010 HAL: hal-01413333v1


Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 - 7. DOI: 10.3389/fmolb.2015.00021 HAL: hal-01429982v1

Linda Payet, Marlène Martinho, Céline Merstorf, Manuela Pastoriza-Gallego, Juan Pelta, Virgile Viasnoff, Loïc Auvray, Murugappan Muthukumar, Jérôme Mathé.
Temperature Effect on Ionic Current and ssDNA Transport through Nanopores.
Biophysical Journal, 2015, 109 (8), 1600 - 1607. DOI: 10.1016/j.bpj.2015.08.043 HAL: hal-01444203v1


Jason England, Jennifer O. Bigelow, Katherine M. Van Heuvelen, Erik R. Farquhar, Marlène Martinho, Katlyn K. Meier, Jonathan R. Frisch, Eckard Münck, Lawrence Que.
An ultra-stable oxoiron(IV) complex and its blue conjugate base.
Chem. Sci., 2014, 5, 1204 - 1215. DOI: 10.1039/C3SC52755G

Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Diversification of EPR signatures in site directed spin labeling using a β-phosphorylated nitroxide.
Phys. Chem. Chem. Phys., 2014, 16, 4202 - 4209. DOI: 10.1039/c3cp54816c HAL: hal-01460516v1


Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 - 1117. DOI: 10.1021/bc4000542 HAL: hal-01460460v1


Marlène Martinho, Johnny Habchi, Zeina El Habre, Léo Nesme, Bruno Guigliarelli, Valérie Belle, Sonia Longhi.
Assessing induced folding within the intrinsically disordered C-terminal domain of the Henipavirus nucleoproteins by site-directed spin labeling EPR spectroscopy.
J. Biomol. Struct. Dyn., 2012, 31, 453-471. DOI: 10.1080/07391102.2012.706068

Johnny Habchi, Marlène Martinho, Antoine Gruet, Bruno Guigliarelli, Sonia Longhi, Valérie Belle.
Monitoring structural transitions in IDPs by site-directed spin-labeling EPR spectroscopy.
Intrinsically Disordered Protein Analysis Volume 1, Methods and Experimental Tools, 2012, 361-386. DOI: 10.1007/978-1-61779-927-3_21

Linda Payet, Marlène Martinho, Manuela Pastoriza-Gallego, Jean-Michel Betton, Loïc Auvray, Juan Pelta, Jérôme Mathé.
Thermal Unfolding of Proteins Probed at the Single Molecule Level Using Nanopores.
Anal. Chem., 2012, 84 (9), 4071 - 4076. DOI: 10.1021/ac300129e


Frédéric Halgand, Johnny Habchi, Laetitia Cravello, Marlène Martinho, Bruno Guigliarelli, Sonia Longhi.
Dividing To Unveil Protein Microheterogeneities: Traveling Wave Ion Mobility Study.
Anal. Chem., 2011, 83 (19), 7306 - 7315. DOI: 10.1021/ac200994c

Magali Lorenzi, Carine Puppo, Régine Lebrun, Sabrina Lignon, Valérie Roubaud, Marlène Martinho, Elisabetta Mileo, Paul Tordo, Sylvain R. A. Marque, Brigitte Gontero, Bruno Guigliarelli, Valérie Belle.
Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins.
Angew. Chem. Int. Ed., 2011, 50, 9108 - 9111. DOI: 10.1002/anie.201102539