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Professeur, Aix-Marseille Université

Page personelle / Personal webpage

Equipe / Group:



Claudio Greco, Vincent Fourmond, Carole Baffert, Po-hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger.
Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes.
Energy Environ. Sci., 2014, 108 (11), 2066 - 3573. DOI: 10.1039/C4EE01848F HAL: hal-01211585v1

Patrick Bertrand (ed).
Electron Paramagnetic Resonance Spectroscopy: Applications.
Electron Paramagnetic Resonance Spectroscopy: Applications, 2014. DOI: 10.1007/978-3-030-39668-8

Julien G.J. Jacques, Vincent Fourmond, Pascal Arnoux, Monique Sabaty, Emilien Etienne, Sandrine Grosse, Frédéric Biaso, Patrick Bertrand, David Pignol, Christophe Léger, Bruno Guigliarelli, Bénédicte Burlat.
Reductive activation in periplasmic nitrate reductase involves chemical modifications of the Mo-cofactor beyond the first coordination sphere of the metal ion.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (2), 277 - 286. DOI: 10.1016/j.bbabio.2013.10.013 HAL: hal-01481487v1


Patrick Bertrand.
Theoretical and Computational Chemistry Series.
2013, 379 - 407. DOI: 10.1039/9781849737753-00379


Abbas Abou Hamdan, Sébastien Dementin, Pierre-Pol Liebgott, Oscar Gutierrez-Sanz, Pierre Richaud, Antonio L. De Lacey, Marc Rousset, Patrick Bertrand, Laurent Cournac, Christophe Léger.
Understanding and Tuning the Catalytic Bias of Hydrogenase.
J. Am. Chem. Soc., 2012, 134 (20), 8368 - 8371. DOI: 10.1021/ja301802r HAL: hal-00748324v1


Carole Baffert, Luca Bertini, Thomas Lautier, Claudio Greco, Kateryna Sybirna, Pierre Ezanno, Emilien Etienne, Philippe Soucaille, Patrick Bertrand, Hervé Bottin, Isabelle Meynial-Salles, Luca De Gioia, Christophe Léger.
CO Disrupts the Reduced H-Cluster of FeFe Hydrogenase. A Combined DFT and Protein Film Voltammetry Study.
J. Am. Chem. Soc., 2011, 133 (7), 2096 - 2099. DOI: 10.1021/ja110627b

Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 - 10221. DOI: 10.1021/ja202615a HAL: hal-00677402v1

Thomas Lautier, Pierre Ezanno, Carole Baffert, Vincent Fourmond, Laurent Cournac, Juan C. Fontecilla-Camps, Philippe Soucaille, Patrick Bertrand, Isabelle Meynial-Salles, Christophe Léger.
The quest for a functional substrate access tunnel in FeFe hydrogenase.
Faraday Discuss., 2011, 304, 55 - 407. DOI: 10.1039/c004099c


Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Émilien Étienne, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants.
Biochemistry, 2010, 49 (11), 2424 - 2432. DOI: 10.1021/bi902140e HAL: hal-00677461

Patrick Bertrand.
Electron Paramagnetic Resonance Spectroscopy: Fundamentals.
Electron Paramagnetic Resonance Spectroscopy: Fundamentals, 2010. DOI: 10.1007/978-3-030-39663-3

Fanny Leroux, Pierre-Pol Liebgott, Laurent Cournac, Pierre Richaud, Arlette Kpebe, Bénedicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Christophe Léger, Marc Rousset.
Is engineering O2-tolerant hydrogenases just a matter of reproducing the active sites of the naturally occurring O2-resistant enzymes?.
International Journal of Hydrogen Energy, 2010, 35 (19), 10770 - 10777. DOI: 10.1016/j.ijhydene.2010.02.071 HAL: hal-01977635v1

Maria-Eirini Pandelia, Vincent Fourmond, Pascale Tron-Infossi, Elisabeth Lojou, Patrick Bertrand, Christophe Léger, Marie-Thérèse Giudici-Orticoni, Wolfgang Lubitz.
Membrane-Bound Hydrogenase I from the Hyperthermophilic Bacterium Aquifex aeolicus: Enzyme Activation, Redox Intermediates and Oxygen Tolerance.
J. Am. Chem. Soc., 2010, 132 (20), 6991 - 7004. DOI: 10.1021/ja910838d HAL: hal-00686880v1

Vincent Fourmond, Monique Sabaty, Pascal Arnoux, Patrick Bertrand, David Pignol, Christophe Léger.
Reassessing the Strategies for Trapping Catalytic Intermediates during Nitrate Reductase Turnover.
J. Phys. Chem. B, 2010, 114 (9), 3341 - 3347. DOI: 10.1021/jp911443y HAL: hal-00677469

Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 275 (1), 113 - 70. DOI: 10.1038/nchembio.276 HAL: hal-01977629

Vincent Fourmond, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Patrick Bertrand, Christophe Léger.
“Two-Step” Chronoamperometric Method for Studying the Anaerobic Inactivation of an Oxygen Tolerant NiFe Hydrogenase.
J. Am. Chem. Soc., 2010, 132 (13), 4848 - 4857. DOI: 10.1021/ja910685j HAL: hal-00677470


Vincent Fourmond, Thomas Lautier, Carole Baffert, Fanny Leroux, Pierre-Pol Liebgott, Sébastien Dementin, Marc Rousset, Pascal Arnoux, David Pignol, Isabelle Meynial-Salles, Phillippe Soucaille, Patrick Bertrand, Christophe Léger.
Correcting for Electrocatalyst Desorption and Inactivation in Chronoamperometry Experiments.
Anal. Chem., 2009, 81 (8), 2962 - 2968. DOI: 10.1021/ac8025702 HAL: hal-01825488v1

Vincent Fourmond, Kevin Hoke, Hendrik A. Heering, Carole Baffert, Fanny Leroux, Patrick Bertrand, Christophe Léger.
SOAS: A free program to analyze electrochemical data and other one-dimensional signals.
Bioelectrochemistry, 2009, 76 (1-2), 141 - 147. DOI: 10.1016/j.bioelechem.2009.02.010 HAL: hal-00402940v1


Christophe Léger, Patrick Bertrand.
Direct Electrochemistry of Redox Enzymes as a Tool for Mechanistic Studies.
Chem. Rev., 2008, 108 (7), 2379 - 2438. DOI: 10.1021/cr0680742 HAL: hal-01211439v1

F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset, C. Leger.
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences, 2008, 53 (Pt 3), 240 - 11193. DOI: 10.1073/pnas.0803689105

Carole Baffert, Marie Demuez, Laurent Cournac, Bénédicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Laurence Girbal, Christophe Léger.
Hydrogen-Activating Enzymes: Activity Does Not Correlate with Oxygen Sensitivity.
Angew. Chem. Int. Ed., 2008, 71 (11), 2777 - 2054. DOI: 10.1002/anie.200704313 HAL: hal-00336018

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Pascal Arnoux, Monique Sabaty, Séverine Boiry, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Major Mo(V) EPR Signature of Rhodobacter sphaeroides Periplasmic Nitrate Reductase Arising from a Dead-End Species That Activates upon Reduction. Relation to Other Molybdoenzymes from the DMSO Reductase Family.
J. Phys. Chem. B, 2008, 112 (48), 15478 - 15486. DOI: 10.1021/jp807092y HAL: hal-00336002


Patrick Bertrand, Bettina Frangioni, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Bruno Guigliarelli, David Pignol, Christophe Léger.
Effects of Slow Substrate Binding and Release in Redox Enzymes:  Theory and Application to Periplasmic Nitrate Reductase.
J. Phys. Chem. B, 2007, 111 (34), 10300 - 10311. DOI: 10.1021/jp074340j HAL: hal-00336030

Pascal Lanciano, Axel Magalon, Patrick Bertrand, Bruno Guigliarelli, Stéphane Grimaldi.
High-Stability Semiquinone Intermediate in Nitrate Reductase A (NarGHI) from Escherichia coli Is Located in a Quinol Oxidation Site Close to Heme bD.
Biochemistry, 2007, 46 (18), 5323 - 5329. DOI: 10.1021/bi700074y HAL: hal-00336035v1

Pascal Lanciano, Adrien Savoyant, Stéphane Grimaldi, Axel Magalon, Bruno Guigliarelli, Patrick Bertrand.
New Method for the Spin Quantitation of [4Fe−4S]+ Clusters with S = 3 / 2. Application to the FS0 Center of the NarGHI Nitrate Reductase from Escherichia coli.
J. Phys. Chem. B, 2007, 111 (48), 13632 - 13637. DOI: 10.1021/jp075243t HAL: hal-00336023v1


M. Gabriela Almeida, Célia M. Silveira, Bruno Guigliarelli, Patrick Bertrand, José J.G. Moura, Isabel Moura, Christophe Léger.
A needle in a haystack: The active site of the membrane-bound complex cytochrome c nitrite reductase.
2006, 128 (2), 180 - 288. DOI: 10.1016/j.febslet.2006.12.023 HAL: hal-00336044

Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 - 5218. DOI: 10.1021/ja060233b HAL: hal-00335157v1

Christophe Léger, Florence Lederer, Bruno Guigliarelli, Patrick Bertrand.
Electron Flow in Multicenter Enzymes:  Theory, Applications, and Consequences on the Natural Design of Redox Chains.
J. Am. Chem. Soc., 2006, 128 (1), 180 - 187. DOI: 10.1021/ja055275z HAL: hal-00336055v1


Stéphane Grimaldi, Pascal Lanciano, Patrick Bertrand, Francis Blasco, Bruno Guigliarelli.
Evidence for an EPR-Detectable Semiquinone Intermediate Stabilized in the Membrane-Bound Subunit NarI of Nitrate Reductase A (NarGHI) from Escherichia coli.
Biochemistry, 2005, 44 (4), 1300 - 1308. DOI: 10.1021/bi048009r

Claude More, Marcel Asso, Guy Roger, Bruno Guigliarelli, Jorge Caldeira, José Moura, Patrick Bertrand.
Study of the Spin−Spin Interactions between the Metal Centers of Desulfovibrio gigas Aldehyde Oxidoreductase:  Identification of the Reducible Sites of the [2Fe-2S] 1+,2+ Clusters.
Biochemistry, 2005, 44 (34), 11628 - 11635. DOI: 10.1021/bi0510025


Bettina Frangioni, Pascal Arnoux, Monique Sabaty, David Pignol, Patrick Bertrand, Bruno Guigliarelli, Christophe Léger.
In Rhodobacter sphaeroides Respiratory Nitrate Reductase, the Kinetics of Substrate Binding Favors Intramolecular Electron Transfer.
J. Am. Chem. Soc., 2004, 126 (5), 1328 - 1329. DOI: 10.1021/ja0384072 HAL: hal-00338609v1

Christophe Léger, Sébastien Dementin, Patrick Bertrand, Marc Rousset, Bruno Guigliarelli.
Inhibition and Aerobic Inactivation Kinetics of Desulfovibrio fructosovorans NiFe Hydrogenase Studied by Protein Film Voltammetry.
J. Am. Chem. Soc., 2004, 126 (38), 12162 - 12172. DOI: 10.1021/ja046548d HAL: hal-00336069