member photo

Directeur de Recherche, CNRS

+33 4 91 16 45 36

Equipe / Group: BIP 06

I am a physicist by training, with a background in chemistry and biology too. I am interested in understanding the mechanism of complex metalloenzymes, with a strong focus on using kinetics. My research interests include CO dehydrogenases, which catalyse the reversible reduction of CO2 to CO, the formate dehydrogenases, which catalyse the reversible reduction of of CO2 to formate, hydrogenases, which catalyse the reversible oxidation of hydrogen, and a number of other enzymes, including molybdenum/tungsten enzymes.

I am also developing QSoas, which is a general purpose scriptable data analysis program with very powerful non-linear least squares fitting procedures. I regularly post about how to use QSoas.

I am a member of the FrenchBIC.

ORCID id: 0000-0001-9837-6214


Andrea Fasano, Aurore Jacq-Bailly, Jeremy Wozniak, Vincent Fourmond, Christophe Léger.
Catalytic Bias and Redox-Driven Inactivation of the Group B FeFe Hydrogenase CpIII.
ACS Catal., 2024, 14 (9), 7001 - 7010. DOI: 10.1021/acscatal.4c01352 HAL: hal-04570861v1 (dataset available here)

Andrea Fasano, Vincent Fourmond, Christophe Léger.
Outer-sphere effects on the O2 sensitivity, catalytic bias and catalytic reversibility of hydrogenases.
Chem. Sci., 2024, 15, 5418 - 5433. DOI: 10.1039/D4SC00691G HAL: hal-04550524v1 (dataset available here)

Anna Aldinio-Colbachini, Alain Grossi, Américo G. Duarte, Jean-Vincent Daurelle, Vincent Fourmond.
Combining a Commercial Mixer with a Wall-Tube Electrode Allows the Arbitrary Control of Concentrations in Protein Film Electrochemistry.
Anal. Chem., 2024, 96 (12), 4868–4875. DOI: 10.1021/acs.analchem.3c05293 HAL: hal-04510311v1

Andrea Fasano, Vincent Fourmond, Christophe Léger.
The difference bidirectionality makes to the kinetic modeling of molecular catalysis.
Curr. Op. Electrochem., 2024, 46, 101489. DOI: 10.1016/j.coelec.2024.101489 HAL: hal-04550686v1

Andrea Fasano, Carole Baffert, Conrad Schumann, Gustav Berggren, James A. Birrell, Vincent Fourmond, Christophe Léger.
Kinetic Modeling of the Reversible or Irreversible Electrochemical Responses of FeFe-Hydrogenases.
J. Am. Chem. Soc., 2024, 146 (2), 1455–1466. DOI: 10.1021/jacs.3c10693 HAL: hal-04372676v1

Ana Rita Oliveira, Cristiano Mota, Guilherme Vilela-Alves, Rita Rebelo Manuel, Neide Pedrosa, Vincent Fourmond, Kateryna Klymanska, Christophe Léger, Bruno Guigliarelli, Maria João Romão, Inês A. Cardoso Pereira.
An allosteric redox switch involved in oxygen protection in a CO2 reductase.
Nat Chem Biol, 2024, 20 (1), 111-119. DOI: 10.1038/s41589-023-01484-2 HAL: hal-04383526 (dataset available here)

Andrea Fasano, Vincent Fourmond, Christophe Léger.
Kinetic modeling of 2e-/1H+ and 2e-/2H+ bidirectional catalytic cycles.
Bioelectrochemistry, 2024, 108511 -. DOI: 10.1016/j.bioelechem.2023.108511 HAL: hal-04284492


Andrea Fasano, Chloé Guendon, Aurore Jacq-Bailly, Arlette Kpebe, Jérémy Wozniak, Carole Baffert, Melisa del Barrio, Vincent Fourmond, Myriam Brugna, Christophe Léger.
A Chimeric NiFe Hydrogenase Heterodimer to Assess the Role of the Electron Transfer Chain in Tuning the Enzyme’s Catalytic Bias and Oxygen Tolerance.
J. Am. Chem. Soc., 2023, 145 (36), 20021–20030. DOI: 10.1021/jacs.3c06895 HAL: hal-04284474

Andrea Fasano, Aurore Bailly, Jeremy Wozniak, Vincent Fourmond, Christophe Léger.
Catalytic bias and redox-driven inactivation of ancestral FeFe hydrogenases from group B2.
bioRxiv, 2023. DOI: 10.1101/2023.06.23.541094 HAL: hal-04290784

Anna Aldinio-Colbachini, Andrea Fasano, Chloé Guendon, Aurore Bailly, Jeremy Wozniak, Carole Baffert, Arlette Kpebe, Christophe Léger, Myriam Brugna, Vincent Fourmond.
Transport limited adsorption experiments give a new lower estimate of the turnover frequency of Escherichia coli hydrogenase 1.
BBA Advances, 2023, 100090 -. DOI: 10.1016/j.bbadva.2023.100090 HAL: hal-04221197

Cheriehan Hessin, Jules Schleinitz, Nolwenn Le Breton, Sylvie Choua, Laurence Grimaud, Vincent Fourmond, Marine Desage-El Murr, Christophe Léger.
Assessing the Extent of Potential Inversion by Cyclic Voltammetry: Theory, Pitfalls, and Application to a Nickel Complex with Redox-Active Iminosemiquinone Ligands.
Inorg. Chem., 2023. DOI: 10.1021/acs.inorgchem.2c04365 HAL: hal-04071135

Marta Meneghello, Alexandre Uzel, Marianne Broc, Rita R Manuel, Axel Magalon, Inês A. C. Pereira, Christophe Léger, Anne Walburger, Vincent Fourmond.
Electrochemical Kinetics Support a Second Coordination Sphere Mechanism in Metal‐Based Formate Dehydrogenase.
Angew Chem Int Ed, 2023. DOI: 10.1002/anie.202212224 HAL: hal-03965660


Andreas Rutz, Chandan K. Das, Andrea Fasano, Jan Jaenecke, Shanika Yadav, Ulf-Peter Apfel, Vera Engelbrecht, Vincent Fourmond, Christophe Léger, Lars V. Schäfer, Thomas Happe.
Increasing the O2 Resistance of the [FeFe]-Hydrogenase CbA5H through Enhanced Protein Flexibility.
ACS Catal., 2022, 856 - 865. DOI: 10.1021/acscatal.2c04031 HAL: hal-03932821

Sven T. Stripp, Benjamin R. Duffus, Vincent Fourmond, Christophe Léger, Silke Leimkühler, Shun Hirota, Yilin Hu, Andrew Jasniewski, Hideaki Ogata, Markus W. Ribbe.
Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.
Chem. Rev., 2022, 122 (14), 11900 - 11973. DOI: 10.1021/acs.chemrev.1c00914 HAL: hal-03741456

Asmaa Hadj Ahmed, Jean-Vincent Daurelle, Vincent Fourmond.
Optimizing the mass transport of wall-tube electrodes for protein film electrochemistry.
Electrochimica Acta, 2022, 403, 139521. DOI: 10.1016/j.electacta.2021.139521 HAL: hal-03485431


Christina Felbek, Federica Arrigoni, David de Sancho, Aurore Jacq-Bailly, Robert B. Best, Vincent Fourmond, Luca Bertini, Christophe Léger.
Mechanism of Hydrogen Sulfide-Dependent Inhibition of FeFe Hydrogenase.
ACS Catal., 2021, 15162 - 15176. DOI: 10.1021/acscatal.1c04838 HAL: hal-03468382

Andrea Fasano, Henrik Land, Vincent Fourmond, Gustav Berggren, Christophe Léger.
Reversible or Irreversible Catalysis of H+/H2 Conversion by FeFe Hydrogenases.
J. Am. Chem. Soc., 2021. DOI: 10.1021/jacs.1c09554 HAL: hal-03445761

Marta Meneghello, Christophe Léger, Vincent Fourmond.
Electrochemical studies of CO2‐reducing metalloenzymes.
Chem. Eur. J., 2021. DOI: 10.1002/chem.202102702 HAL: hal-03358088

Matteo Sensi, Carole Baffert, Vincent Fourmond, Luca De Gioia, Luca Bertini, Christophe Léger.
Photochemistry and photoinhibition of the H-cluster of FeFe-hydrogenases.
Sustainable Energy Fuels, 2021, 5, 4248-4260. DOI: 10.1039/D1SE00756D HAL: hal-03358063

Vincent Fourmond, Christophe Léger.
Theoretical Understanding of the Penetration of O2 in Enzymatic Redox Polymer Films: The Case of Unidirectional Catalysis and Irreversible Inactivation in a Film of Arbitrary Thickness.
ChemElectroChem, 2021, 8 (13), 2607 - 2615. DOI: 10.1002/celc.202100586 HAL: hal-03277147v1

Vincent Fourmond, Nicolas Plumeré, Christophe Léger.
Reversible catalysis.
Nat Rev Chem, 2021, 5 (5). DOI: 10.1038/s41570-021-00268-3 HAL: hal-03215154

Steffen Hardt, Stefanie Stapf, Dawit T. Filmon, James A. Birrell, Olaf Rüdiger, Vincent Fourmond, Christophe Léger, Nicolas Plumeré.
Reversible H2 oxidation and evolution by hydrogenase embedded in a redox polymer film.
Nature Catalysis, 2021, 4, 251-258. DOI: 10.1038/s41929-021-00586-1 HAL: hal-03215114

Marta Meneghello, Ana Rita Oliveira, Aurore Jacq-Bailly, Inês Cardoso Peirera, Christophe Léger, Vincent Fourmond.
Formate dehydrogenases reduce CO2 rather than HCO3‐: an electrochemical demonstration.
Angew. Chem. Int. Ed., 2021. DOI: 10.1002/anie.202101167 HAL: hal-03181024

Martin Winkler, Jifu Duan, Andreas Rutz, Christina Felbek, Lisa Scholtysek, Oliver Lampret, Jan Jaenecke, Ulf-Peter Apfel, Gianfranco Gilardi, Francesca Valetti, Vincent Fourmond, Eckhard Hofmann, Christophe Léger, Thomas Happe.
A safety cap protects hydrogenase from oxygen attack.
Nat Commun, 2021, 12 (1). DOI: 10.1038/s41467-020-20861-2 HAL: hal-03129104

Aurore Jacq-Bailly, Martino Benvenuti, Natalie Payne, Arlette Kpebe, Christina Felbek, Vincent Fourmond, Christophe Léger, Myriam Brugna, Carole Baffert.
Electrochemical Characterization of a Complex FeFe Hydrogenase, the Electron-Bifurcating Hnd From Desulfovibrio fructosovorans.
Front. Chem., 2021, 8. DOI: 10.3389/fchem.2020.573305 HAL: hal-03103487v1

Christina Felbek, Steffen Hardt, Cecilia Papini, Debajyoti Pramanik, Vincent Artero, Marc Fontecave, Vincent Fourmond, Nicolas Plumere, Christophe Léger.
Artificial maturation of [FeFe] hydrogenase in a redox polymer film.
Chem. Commun., 2021. DOI: 10.1039/D0CC08168J HAL: hal-03102775v1


Vincent Fourmond, Christophe Léger.
Numerical computations of Marcus–hush–Chidsey electron transfer rate constants.
Journal of Electroanalytical Chemistry, 2020, 114762 -. DOI: 10.1016/j.jelechem.2020.114762 HAL: hal-02968022

Elizabeth Charlotte Wittenborn, Chloé Guendon, Mériem Merrouch, Martino Benvenuti, Vincent Fourmond, Christophe Leger, Catherine L. Drennan, and Sébastien Dementin.
The solvent-exposed Fe-S D-cluster contributes to oxygen-resistance in D. vulgaris Ni-Fe carbon monoxide dehydrogenase.
ACS Cat., 2020, 10, 7328–7335. DOI: 10.1021/acscatal.0c00934 HAL: hal-02936282

Martino Benvenuti, Marta Meneghello, Chloé Guendon, Aurore Jacq-Bailly, Jae-hun Jeoung, Holger Dobbek, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
The two CO-dehydrogenases of Thermococcus sp. AM4.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 148188 -. DOI: 10.1016/j.bbabio.2020.148188 HAL: hal-02537797


Huaiguang Li, Darren Buesen, Sébastien Dementin, Christophe Léger, Vincent Fourmond, Nicolas Plumeré.
Complete Protection of O2-Sensitive Catalysts in Thin Films.
J. Am. Chem. Soc., 2019, 141 (42), 16734 - 16742. DOI: 10.1021/jacs.9b06790 HAL: hal-02369997v1

N. L. Costa, B. Hermann, V. Fourmond, M. M. Faustino, M. Teixeira, O. Einsle, C. M. Paquete, R. O. Louro.
How Thermophilic Gram-Positive Organisms Perform Extracellular Electron Transfer: Characterization of the Cell Surface Terminal Reductase OcwA.
mBio, 2019, 226 (4), 269 -. DOI: 10.1128/mBio.01210-19 HAL: hal-02315696v1

Melisa Barrio, Vincent Fourmond.
Redox (In)activations of Metalloenzymes: A Protein Film Voltammetry Approach.
ChemElectroChem, 2019, 260 (19), 14701 - 4962. DOI: 10.1002/celc.201901028 HAL: hal-02308474v1

Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira.
A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase.
ACS Catal., 2019, 9 (9), 8509 - 8519. DOI: 10.1021/acscatal.9b02347 HAL: hal-02278341v1

Elizabeth C. Wittenborn, Steven E. Cohen, Mériem Merrouch, Christophe Léger, Vincent Fourmond, Sébastien Dementin, Catherine L. Drennan.
Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase.
J. Biol. Chem., 2019, 257 (35), 6259 - 13026. DOI: 10.1074/jbc.RA119.009610 HAL: hal-02188452v1

Vincent Fourmond, Eric S. Wiedner, Wendy J. Shaw, Christophe Léger.
Understanding and Design of Bidirectional and Reversible Catalysts of Multielectron, Multistep Reactions.
J. Am. Chem. Soc., 2019, 141 (28), 11269 - 11285. DOI: 10.1021/jacs.9b04854 HAL: hal-02179792v1

Kamal Zeamari, Guillaume Gerbaud, Sandrine Grosse, Vincent Fourmond, Florence Chaspoul, Frédéric Biaso, Pascal Arnoux, Monique Sabaty, David Pignol, Bruno Guigliarelli, Bénédicte Burlat.
Tuning the redox properties of a [4Fe-4S] center to modulate the activity of Mo-bisPGD periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2019, 1860 (5), 402 - 413. DOI: 10.1016/j.bbabio.2019.01.003 HAL: hal-02106668v1

Melisa del Barrio, Chloé Guendon, Arlette Kpebe, Carole Baffert, Vincent Fourmond, Myriam Brugna, Christophe Léger.
Valine-to-Cysteine Mutation Further Increases the Oxygen Tolerance of Escherichia coli NiFe Hydrogenase Hyd-1.
ACS Catal., 2019, 9 (5), 4084 - 4088. DOI: 10.1021/acscatal.9b00543 HAL: hal-02104593v1

Mariam Fadel, Jean-Vincent Daurelle, Vincent Fourmond, Jérôme Vicente.
A new electrochemical cell with a uniformly accessible electrode to study fast catalytic reactions.
Phys. Chem. Chem. Phys., 2019, 82 (23), 3124 - 12371. DOI: 10.1039/c9cp01487j HAL: hal-02308533v1


Elizabeth C Wittenborn, Mériem Merrouch, Chie Ueda, Laura Fradale, Christophe Léger, Vincent Fourmond, Maria-Eirini Pandelia, Sébastien Dementin, Catherine L Drennan.
Redox-dependent rearrangements of the NiFeS cluster of carbon monoxide dehydrogenase.
2018, 119, 13690 -. DOI: 10.7554/eLife.39451 HAL: hal-01917213v1

Mariam Fadel, Jean-Vincent Daurelle, Jérôme Vicente, Vincent Fourmond.
Impact of alignment defects of rotating disk electrode on transport properties.
Electrochimica Acta, 2018, 269, 534 - 543. DOI: 10.1016/j.electacta.2018.01.125 HAL: hal-01790797v1

Giorgio Caserta, Cecilia Papini, Agnieszka Adamska-Venkatesh, Ludovic Pecqueur, Constanze Sommer, Edward Reijerse, Wolfgang Lubitz, Charles Gauquelin, Isabelle Meynial-Salles, Debajyoti Pramanik, Vincent Artero, Mohamed Atta, Melisa del Barrio, Bruno Faiv.
Engineering an [FeFe]-Hydrogenase: Do Accessory Clusters Influence O 2 Resistance and Catalytic Bias?.
J. Am. Chem. Soc., 2018, 140 (16), 5516 - 5526. DOI: 10.1021/jacs.8b01689 HAL: hal-01759288v1

Melisa del Barrio, Matteo Sensi, Laura Fradale, Maurizio Bruschi, Claudio Greco, Luca de Gioia, Luca Bertini, Vincent Fourmond, Christophe Léger.
Interaction of the H-Cluster of FeFe Hydrogenase with Halides.
J. Am. Chem. Soc., 2018, 140 (16), 5485 - 5492. DOI: 10.1021/jacs.8b01414 HAL: hal-01759718v1

Melisa del Barrio, Matteo Sensi, Christophe Orain, Carole Baffert, Sébastien Dementin, Vincent Fourmond, Christophe Léger.
Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels.
Acc. Chem. Res., 2018, 51 (3), 769 - 777. DOI: 10.1021/acs.accounts.7b00622 HAL: hal-01745738v1

Mériem Merrouch, Martino Benvenuti, Marco Lorenzi, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
Maturation of the [Ni–4Fe–4S] active site of carbon monoxide dehydrogenases.
J Biol Inorg Chem, 2018, 37 (4), 80 - 620. DOI: 10.1007/s00775-018-1541-0 HAL: hal-01816335v1

Matteo Sensi, Christophe Orain, Carole Baffert, Vincent Fourmond et Christophe Léger.
La réaction des hydrogénases FeFe avec le dioxygène: étude expérimentale et théorique.
L'Actualité Chimique, 2018 (426). (voir / see)

Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, David Guieysse, Laurence Girbal, Vincent Fourmond, Isabelle André, Bruno Guigliarelli, Christophe Léger, Philippe Soucaille, Isabelle Meynial-Salles.
Roles of the F-domain in [FeFe] hydrogenase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2018, 1859 (2), 69 - 77. DOI: 10.1016/j.bbabio.2017.08.010 HAL: cea-01950818

V. Fourmond.
Direct Electrochemistry of Molybdenum and Tungsten Enzymes.
Encyclopedia of Interfacial Chemistry, 2018, 477 - 488. DOI: 10.1016/B978-0-12-409547-2.13354-2 HAL: hal-01614270v1


Vincent Fourmond.
L’électroenzymologie, un outil pour étudier les enzymes redox.
L'Actualité Chimique, 2017 (424).

Lilith Domnik, Meriem Merrouch, Sebastian Goetzl, Jae-Hun Jeoung, Christophe Léger, Sébastien Dementin, Vincent Fourmond, Holger Dobbek.
CODH-IV: A High-Efficiency CO-Scavenging CO Dehydrogenase with Resistance to O2.
Angew. Chem. Int. Ed., 2017, 121 (48), 11034 - 15469. DOI: 10.1002/anie.201709261 HAL: hal-01696167v1

Matteo Sensi, Melisa del Barrio, Carole Baffert, Vincent Fourmond, Christophe Léger.
New perspectives in hydrogenase direct electrochemistry.
Current Opinion in Electrochemistry, 2017, 5 (1), 135 - 145. DOI: 10.1016/j.coelec.2017.08.005 HAL: hal-01614142v1

Matteo Sensi, Carole Baffert, Laura Fradale, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Maurizio Bruschi, Vincent Fourmond, Christophe Léger, Luca Bertini.
Photoinhibition of FeFe Hydrogenase.
ACS Catal., 2017, 7 (10), 7378 - 7387. DOI: 10.1021/acscatal.7b02252 HAL: hal-01613876v1

Ting Zeng, Silke Leimkühler, Ulla Wollenberger, Vincent Fourmond.
Transient Catalytic Voltammetry of Sulfite Oxidase Reveals Rate Limiting Conformational Changes.
J. Am. Chem. Soc., 2017, 139 (33), 11559 - 11567. DOI: 10.1021/jacs.7b05480 HAL: hal-01614177v1

Meriem Merrouch, Jessica Hadj-Saïd, Christophe Léger, Sébastien Dementin, Vincent Fourmond.
Reliable estimation of the kinetic parameters of redox enzymes by taking into account mass transport towards rotating electrodes in protein film voltammetry experiments.
Electrochimica Acta, 2017, 245, 1059 - 1064. DOI: 10.1016/j.electacta.2017.03.114 HAL: hal-01614234v1

Vincent Fourmond, Christophe Léger.
Dinitrogen Reduction: Interfacing the Enzyme Nitrogenase with Electrodes.
Angew. Chem. Int. Ed., 2017, 137 (16), 5494 - 4390. DOI: 10.1002/anie.201701179 HAL: hal-01514839v1

Vincent Fourmond, Christophe Léger.
Modelling the voltammetry of adsorbed enzymes and molecular catalysts.
Current Opinion in Electrochemistry, 2017, 1 (1), 110 - 120. DOI: 10.1016/j.coelec.2016.11.002 HAL: hal-01440516v1

Adam Kubas, Christophe Orain, David De Sancho, Laure Saujet, Matteo Sensi, Charles Gauquelin, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Carole Baffert, Vincent Fourmond, Robert B. Best, Jochen Blumberger, Christophe Léger.
Mechanism of O2 diffusion and reduction in FeFe hydrogenases.
Nature Chem, 2017, 11 (1), 205 - 95. DOI: 10.1038/nchem.2592 HAL: hal-01406025v1


Matteo Sensi, Carole Baffert, Claudio Greco, Giorgio Caserta, Charles Gauquelin, Laure Saujet, Marc Fontecave, Souvik Roy, Vincent Artero, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Vincent Fourmond, Christophe Léger, Luca B.
Reactivity of the Excited States of the H-Cluster of FeFe Hydrogenases.
J. Am. Chem. Soc., 2016, 138 (41), 13612 - 13618. DOI: 10.1021/jacs.6b06603 HAL: hal-01889471v1

Thibaut Molle, Yohann Moreau, Martin Clemancey, Farhad Forouhar, Jean-Luc Ravanat, Nicolas Duraffourg, Vincent Fourmond, Jean-Marc Latour, Serge Gambarelli, Etienne Mulliez, Mohamed Atta.
Redox Behavior of the S -Adenosylmethionine (SAM)-Binding Fe–S Cluster in Methylthiotransferase RimO, toward Understanding Dual SAM Activity.
Biochemistry, 2016, 55 (41), 5798 - 5808. DOI: 10.1021/acs.biochem.6b00597 HAL: hal-01414834v1

Vincent Fourmond.
QSoas: A Versatile Software for Data Analysis.
Anal. Chem., 2016, 88 (10), 5050 - 5052. DOI: 10.1021/acs.analchem.6b00224 HAL: hal-01414965v1

V. Fourmond, C. Léger.
Advances in Biochemical Engineering/Biotechnology.
2016, 48, 1400 - 41. DOI: 10.1007/10_2015_5016 HAL: hal-01413237v1


Jessica Hadj-Saïd, Maria-Eirini Pandelia, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
The Carbon Monoxide Dehydrogenase from Desulfovibrio vulgaris.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (12), 1574 - 1583. DOI: 10.1016/j.bbabio.2015.08.002 HAL: hal-01430965v1

Meriem Merrouch, Jessica Hadj-Saïd, Lilith Domnik, Holger Dobbek, Christophe Léger, Sébastien Dementin, Vincent Fourmond.
O2 Inhibition of Ni-Containing CO Dehydrogenase Is Partly Reversible.
Chem. Eur. J., 2015, 181 (52), 437 - 18938. DOI: 10.1002/chem.201502835 HAL: hal-01432205v1

Pierre Ceccaldi, Julia Rendon, Christophe Léger, René Toci, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi, Vincent Fourmond.
Reductive activation of E. coli respiratory nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (10), 1055 - 1063. DOI: 10.1016/j.bbabio.2015.06.007 HAL: hal-01429839v1

Christophe Orain, Laure Saujet, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Carole Baffert, Vincent Fourmond, Hervé Bottin, Christophe Léger.
Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 2 Demonstrate That the Reaction Is Partly Reversible.
J. Am. Chem. Soc., 2015, 137 (39), 12580 - 12587. DOI: 10.1021/jacs.5b06934 HAL: hal-01211469v1

Vincent Fourmond, Stefanie Stapf, Huaiguang Li, Darren Buesen, James Birrell, Olaf Rüdiger, Wolfgang Lubitz, Wolfgang Schuhmann, Nicolas Plumeré, Christophe Léger.
Mechanism of Protection of Catalysts Supported in Redox Hydrogel Films.
J. Am. Chem. Soc., 2015, 137 (16), 5494 - 5505. DOI: 10.1021/jacs.5b01194 HAL: hal-01428049v1

Vincent Fourmond, Carole Baffert, Sébastien Dementin, Christophe Léger.
L’électrochimie, un outil pour étudier les mécanismes enzymatiques.
L'Actualité Chimique, 2015 (392).

Pierre Ceccaldi, Marta C. Marques, Vincent Fourmond, Inês Cardoso Pereira, Christophe Léger.
Oxidative inactivation of NiFeSe hydrogenase.
Chem. Commun., 2015, 562 (75), 43 - 14226. DOI: 10.1039/C5CC05930E HAL: hal-01211493v1


Julien G.J. Jacques, Bénédicte Burlat, Pascal Arnoux, Monique Sabaty, Bruno Guigliarelli, Christophe Léger, David Pignol, Vincent Fourmond.
Kinetics of substrate inhibition of periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (10), 1801 - 1809. DOI: 10.1016/j.bbabio.2014.05.357 HAL: hal-01494456v1

Vincent Fourmond, Claudio Greco, Kateryna Sybirna, Carole Baffert, Po-Hung Wang, Pierre Ezanno, Marco Montefiori, Maurizio Bruschi, Isabelle Meynial-Salles, Philippe Soucaille, Jochen Blumberger, Hervé Bottin, Luca De Gioia, Christophe Léger.
The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster.
Nature Chem, 2014, 130 (4), 4533 - 342. DOI: 10.1038/nchem.1892 HAL: hal-01481520v1

Julien G.J. Jacques, Vincent Fourmond, Pascal Arnoux, Monique Sabaty, Emilien Etienne, Sandrine Grosse, Frédéric Biaso, Patrick Bertrand, David Pignol, Christophe Léger, Bruno Guigliarelli, Bénédicte Burlat.
Reductive activation in periplasmic nitrate reductase involves chemical modifications of the Mo-cofactor beyond the first coordination sphere of the metal ion.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (2), 277 - 286. DOI: 10.1016/j.bbabio.2013.10.013 HAL: hal-01481487v1

Claudio Greco, Vincent Fourmond, Carole Baffert, Po-hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger.
Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes.
Energy Environ. Sci., 2014, 108 (11), 2066 - 3573. DOI: 10.1039/C4EE01848F HAL: hal-01211585v1

Viviane Hajj, Carole Baffert, Kateryna Sybirna, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Vincent Fourmond, Christophe Léger.
FeFe hydrogenase reductive inactivation and implication for catalysis.
Energy Environ. Sci., 2014, 6 (2), 2460 - 719. DOI: 10.1039/C3EE42075B HAL: hal-01481475v1


Toshiko Miyake, Maurizio Bruschi, Ugo Cosentino, Carole Baffert, Vincent Fourmond, Christophe Léger, Giorgio Moro, Luca De Gioia, Claudio Greco.
Does the environment around the H-cluster allow coordination of the pendant amine to the catalytic iron center in [FeFe] hydrogenases? Answers from theory.
J Biol Inorg Chem, 2013, 13 (6), 3580 - 700. DOI: 10.1007/s00775-013-1014-4 HAL: hal-03023214

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Thomas Lautier, Abbas Abou Hamdan, Sébastien Dementin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Christophe Léger.
Steady-State Catalytic Wave-Shapes for 2-Electron Reversible Electrocatalysts and Enzymes.
J. Am. Chem. Soc., 2013, 135 (10), 3926 - 3938. DOI: 10.1021/ja311607s HAL: hal-01268145v1

Aurélie Jacques, Martin Clémancey, Geneviève Blondin, Vincent Fourmond, Jean-Marc Latour, Olivier Sénèque.
A cyclic peptide-based redox-active model of rubredoxin.
Chem. Commun., 2013, 16 (28), 501 -. DOI: 10.1039/C3CC40517F HAL: hal-01069592v1

Sigolène Canaguier, Vincent Fourmond, Carlo U. Perotto, Jennifer Fize, Jacques Pécaut, Marc Fontecave, Martin J. Field, Vincent Artero.
Catalytic hydrogen production by a Ni–Ru mimic of NiFe hydrogenases involves a proton-coupled electron transfer step.
Chem. Commun., 2013, 46 (44), 8942 -. DOI: 10.1039/C3CC40987B HAL: hal-01069157v1

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Sébastien Dementin, Abbas Abou-Hamdan, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
The mechanism of inhibition by H2 of H2-evolution by hydrogenases.
Chem. Commun., 2013, 109 (61), 19916 -. DOI: 10.1039/C3CC43297A HAL: hal-01268212v1


A. Abou Hamdan, P.-P. Liebgott, V. Fourmond, O. Gutierrez-Sanz, A. L. De Lacey, P. Infossi, M. Rousset, S. Dementin, C. Leger.
Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants.
Proceedings of the National Academy of Sciences, 2012, 76 (1-2), 141 - 19921. DOI: 10.1073/pnas.1212258109 HAL: hal-01825482v1

Saioa Cobo, Jonathan Heidkamp, Pierre-André Jacques, Jennifer Fize, Vincent Fourmond, Laure Guetaz, Bruno Jousselme, Valentina Ivanova, Holger Dau, Serge Palacin, Marc Fontecave, Vincent Artero.
A Janus cobalt-based catalytic material for electro-splitting of water.
Nature Mater, 2012, 440 (9), 295 - 807. DOI: 10.1038/nmat3385 HAL: cea-00960632


Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 - 10221. DOI: 10.1021/ja202615a HAL: hal-00677402v1

Vincent Fourmond, Sigolène Canaguier, Benjamin Golly, Martin J. Field, Marc Fontecave, Vincent Artero.
A nickel–manganese catalyst as a biomimic of the active site of NiFe hydrogenases: a combined electrocatalytical and DFT mechanistic study.
Energy Environ. Sci., 2011, 76 (7), 141 -. DOI: 10.1039/C0EE00736F HAL: hal-01139836v1

Thomas Lautier, Pierre Ezanno, Carole Baffert, Vincent Fourmond, Laurent Cournac, Juan C. Fontecilla-Camps, Philippe Soucaille, Patrick Bertrand, Isabelle Meynial-Salles, Christophe Léger.
The quest for a functional substrate access tunnel in FeFe hydrogenase.
Faraday Discuss., 2011, 304, 55 - 407. DOI: 10.1039/c004099c HAL: hal-02646834


Vincent Fourmond, Pierre-André Jacques, Marc Fontecave, Vincent Artero.
H2 Evolution and Molecular Electrocatalysts: Determination of Overpotentials and Effect of Homoconjugation.
Inorg. Chem., 2010, 49 (22), 10338 - 10347. DOI: 10.1021/ic101187v HAL: hal-01069160v1

Maria-Eirini Pandelia, Vincent Fourmond, Pascale Tron-Infossi, Elisabeth Lojou, Patrick Bertrand, Christophe Léger, Marie-Thérèse Giudici-Orticoni, Wolfgang Lubitz.
Membrane-Bound Hydrogenase I from the Hyperthermophilic Bacterium Aquifex aeolicus: Enzyme Activation, Redox Intermediates and Oxygen Tolerance.
J. Am. Chem. Soc., 2010, 132 (20), 6991 - 7004. DOI: 10.1021/ja910838d HAL: hal-00686880v1

Vincent Fourmond, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Patrick Bertrand, Christophe Léger.
“Two-Step” Chronoamperometric Method for Studying the Anaerobic Inactivation of an Oxygen Tolerant NiFe Hydrogenase.
J. Am. Chem. Soc., 2010, 132 (13), 4848 - 4857. DOI: 10.1021/ja910685j HAL: hal-00677470

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Émilien Étienne, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants.
Biochemistry, 2010, 49 (11), 2424 - 2432. DOI: 10.1021/bi902140e HAL: hal-00677461

Vincent Fourmond, Monique Sabaty, Pascal Arnoux, Patrick Bertrand, David Pignol, Christophe Léger.
Reassessing the Strategies for Trapping Catalytic Intermediates during Nitrate Reductase Turnover.
J. Phys. Chem. B, 2010, 114 (9), 3341 - 3347. DOI: 10.1021/jp911443y HAL: hal-00677469

Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 6, 63 - 70. DOI: 10.1038/nchembio.276 HAL: hal-01977629


Vincent Fourmond, Kevin Hoke, Hendrik A. Heering, Carole Baffert, Fanny Leroux, Patrick Bertrand, Christophe Léger.
SOAS: A free program to analyze electrochemical data and other one-dimensional signals.
Bioelectrochemistry, 2009, 76 (1-2), 141 - 147. DOI: 10.1016/j.bioelechem.2009.02.010 HAL: hal-00402940v1

Vincent Fourmond, Thomas Lautier, Carole Baffert, Fanny Leroux, Pierre-Pol Liebgott, Sébastien Dementin, Marc Rousset, Pascal Arnoux, David Pignol, Isabelle Meynial-Salles, Phillippe Soucaille, Patrick Bertrand, Christophe Léger.
Correcting for Electrocatalyst Desorption and Inactivation in Chronoamperometry Experiments.
Anal. Chem., 2009, 81 (8), 2962 - 2968. DOI: 10.1021/ac8025702 HAL: hal-01825488v1


Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Pascal Arnoux, Monique Sabaty, Séverine Boiry, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Major Mo(V) EPR Signature of Rhodobacter sphaeroides Periplasmic Nitrate Reductase Arising from a Dead-End Species That Activates upon Reduction. Relation to Other Molybdoenzymes from the DMSO Reductase Family.
J. Phys. Chem. B, 2008, 112 (48), 15478 - 15486. DOI: 10.1021/jp807092y HAL: hal-00336002

Kateryna Sybirna, Tatiana Antoine, Pia Lindberg, Vincent Fourmond, Marc Rousset, Vincent Méjean, Hervé Bottin.
Shewanella oneidensis: a new and efficient System for Expression and Maturation of heterologous [Fe-Fe] Hydrogenase from Chlamydomonas reinhardtii.
BMC Biotechnology, 2008, 8 (1), 73 -. DOI: 10.1186/1472-6750-8-73 HAL: hal-00331156v1


Vincent Fourmond, Bernard Lagoutte, Pierre Sétif, Winfried Leibl, Christophe Demaille.
Electrochemical Study of a Reconstituted Photosynthetic Electron-Transfer Chain.
J. Am. Chem. Soc., 2007, 129 (29), 9201 - 9209. DOI: 10.1021/ja0714787 HAL: hal-00207644