Electron Paramagnetic Resonance Facility
in Life Sciences at the Chemistry-Biology interface

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Interests of EPR in Life Sciences

  • Structural analysis at the atomic and molecular level of biological systems.
  • Study of the function of complex redox enzymes.
  • Protein dynamics.
  • Protein-protein interactions.

Methodology

  • Determination of the metal content.
  • Analysis of metal cofactors.
  • Detection of free radicals (oxidative stress, dosimetry, enzymatic mechanisms).
  • Insertion of paramagnetic labels → Biostructural EPR.

Applications

  • Microbial bioenergetic metabolism.
  • Molecular sensitivity to stress factors (oxidative, thermal, chemical stress).
  • Enzymatic engineering.
  • Mapping molecular interactions between physiological partners.
  • Study of structural transitions and folding / unfolding events.
  • Organization of large biological assemblages.
  • In-cell biostructural EPR.

Users

  • Academics in Biology, Chemistry and Physics.
  • Open to private companies.