Post-doct: Flow NMR for exploring photosynthetic enzymatic regulation on model microalgae

Context: We are expert enzymologists and are working on microalgae. We are currently developing the use of NMR to monitor in real-time the physico-chemical and molecular transitions upon the dark/light transition at the origin of the regulation of CO2 assimilation by microalgae. The group has described the molecular mechanism responsible for the dark/light regulation of photosynthesis. We have described the molecular transitions (redox potential, structural transitions) of the intrinsically disordered protein CP12 at the origin of this regulation using NMR. Nevertheless, these effects remain challenging to monitor in real-time, and our preliminary results strongly indicate that flow-NMR is the appropriate tool to allow such study. NMR has been used to study fermentation regulation in-situ, but the study of photosynthesis regulation requires that the algae are maintained in aerobic, non-limiting [CO2] and light conditions. These conditions will be provided by newly acquired flow-NMR systems.

In the context of the recently funded ANR PhotOxIN project, we are recruiting a highly motivated post-doc to monitor in real time the structural transition of CP12, as well as the production/consumption of key photosynthesis metabolites upon a dark/light transition on cell extract or intact microalgae in aerobic and non-limiting [CO2] conditions. Different mutant strains of algae are available in the lab. The post-doc will develop the flow-NMR, and have a routine access to a 600 MHz spectrometer equipped with a cryo-probe, and access to different algal cultures.

The post-doc will benefit from our network of collaborator experts in NMR metabolomic (Mehdi Yemloul, ISM2, Marseille, France) and in NMR methods development and flow-NMR (Jonathan Farjon, CEISAM, Nantes, France), and several visiting periods in these labs are planned to ease the transfer of technology.

Profile: Candidates shall have a Ph. D. in (Bio)Chemical analysis or Chemical Physics and have demonstrated a solid track record in NMR. She/he should be open-minded due to the interdisciplinary and collaborative aspects of the project. She/he will present her/his work in national and international conferences and good skills in English are required. She/he will be highly encouraged to participate in academic (publications, conferences) and non-academic valorization (dissemination). Candidates shall have good skills and master the use of flow-NMR. Expertise on NMR methods development is welcomed. Knowledge on enzymology, photosynthesis or protein structure will be considered as a plus. Position available from Spring 2023, actual funding is for 2 years, possibility to envisage funding beyond this period. The net monthly salary is between 2000 € and 2500 €.

Application procedure:

Candidates shall submit their complete Curriculum Vitae, a motivation letter as well as two recommendation letters to:

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Selected publications (visit our website for more):

1. Gérard, C.; Lebrun, R.; Lemesle, E.; Avilan, L.; Chang, K.S.; Jin, E.; Carrière, F.; Gontero, B.; Launay, H. Reduction in Phosphoribulokinase Amount and Re-Routing Metabolism in Chlamydomonas Reinhardtii CP12 Mutants. International Journal of Molecular Sciences 2022, 23, 2710, doi:10.3390/ijms23052710.

2. Launay, H.; Shao, H.; Bornet, O.; Cantrelle, F.-X.; Lebrun, R.; Receveur-Brechot, V.; Gontero, B. Flexibility of Oxidized and Reduced States of the Chloroplast Regulatory Protein CP12 in Isolation and in Cell Extracts. Biomolecules 2021, 11, 701, doi:10.3390/biom11050701.

3. Piersanti, E.; Rezig, L.; Tranchida, F.; El-Houri, W.; Abagana, S.M.; Campredon, M.; Shintu, L.; Yemloul, M. Evaluation of the Rotating-Frame Relaxation (T1ρ) Filter and Its Application in Metabolomics as an Alternative to the Transverse Relaxation (T2) Filter. Anal. Chem. 2021, 93, 8746–8753.

4. Bouillaud, D.; Drouin, D.; Charrier, B.; Jacquemmoz, C.; Farjon, J.; Giraudeau, P.; Gonçalves, O. Using Benchtop NMR Spectroscopy as an Online Non-Invasive in Vivo Lipid Sensor for Microalgae Cultivated in Photobioreactors. Process Biochemistry 2020, 93, 63–68.

Contact: Hélène LAUNAY

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