Directrice de Recherche, CNRS
+33 4 91 16 40 97
Chef d'équipe / Group leader: BIP 09
Sujet de Recherche :
Les substrats énergétiques utilisés par les procaryotes sont extrêmement divers et comprennent même des substances toxiques pour la plupart des organismes. L’arsenic apparait ainsi de plus en plus clairement comme un substrat microbien bioénergétique majeur, utilisé dès l’archéen. Notre objectif de recherche est non seulement de comprendre la place du métabolisme de l’Arsenic dans l’évolution globale des processus énergétiques et de la vie sur terre, mais également de décortiquer les mystères encore nombreux des mécanismes moléculaires du fonctionnement des enzymes impliquées dans le métabolisme énergétique de l’Arsenic : arsénite oxydase Aio, arsénite oxydase alternative Arx et arséniate reductase Arr. Ces questions sont abordées selon la spécificité de notre équipe BIP09, c’est-à-dire l’approche phylogénétique conjointe à l’expérimentation biochimique (enzymologie, purifications), biophysique (spectroscopie optique et RPE) et microbiologique. Celles-ci nous amènent à étudier 1) les déterminants moléculaires des propriétés d’autres enzymes à Molybdène (Mo) que celles impliquées dans le métabolisme de l’Arsenic, comme la TMAO réductase TorA, la Sulfite oxydase Soe 2) la phylogénie d’enzymes autres que celles du métabolisme de l’Arsenic, en lien avec celui-ci, par exemple les enzymes du métabolisme du soufre ou du nitrate.
Publications
ORCID id: 0000-0002-6820-4478
2024
Giulia D'Ermo, Stéphane Audebert, Luc Camoin, Britta Planer‐Friedrich, Corinne Casiot‐Marouani, Sophie Delpoux, Régine Lebrun, Marianne Guiral, Barbara Schoepp‐Cothenet.
Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila.
Environmental Microbiology, 2024, 26 (6), - .
DOI: 10.1111/1462-2920.16655
HAL: hal-04620414
Giulia D’Ermo, Marianne Guiral, Barbara Schoepp-Cothenet.
The complex interplay of sulfur and arsenic bioenergetic metabolisms in the arsenic geochemical cycle.
Geomicrobiology, Geomicrobiology: Natural and anthropogenic settings (Lucian C. Staicu Larry L. Barton Eds), 2024 (ISBN:Hardcover: 978-3-031-54305-0; Ebook: 978-3-031-54306-7 ).
HAL: hal-04410384
2022
Schoepp-Cothenet Barbara, Magalon Axel.
EBEC 2020: The conference that should have taken place!.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863 (1), 148502 -.
DOI: 10.1016/j.bbabio.2021.148502
HAL: hal-03455615v1
2021
Simon Duval, Kilian Zuchan, Frauke Baymann, Barbara Schoepp-Cothenet, Elbert Branscomb, Michael J. Russell, Wolfgang Nitschke.
Minerals and the emergence of life.
MILS, Metals, Microbes, and Minerals - The Biogeochemical Side of Life, 2021, 21, 135-157.
DOI: 10.1515/9783110589771-011
HAL: hal-03163239v1
Karl J. Koebke, Toni Kühl, Elisabeth Lojou, Borries Demeler, Barbara Schoepp-Cothenet, Olga Iranzo, Vincent L. Pecoraro, Anabella Ivancich.
The pH‐Induced Selectivity Between Cysteine or Histidine Coordinated Heme in an Artificial α‐Helical Metalloprotein.
Angew. Chem. Int. Ed., 2021, 60 (8), 3974 - 3978.
DOI: 10.1002/anie.202012673
HAL: hal-03017501
2020
Souhela Boughanemi, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Barbara Schoepp-Cothenet, Marianne Guiral.
Sulfite oxidation by the quinone-reducing molybdenum sulfite dehydrogenase SoeABC from the bacterium Aquifex aeolicus.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 1861 (11), 148279 -.
DOI: 10.1016/j.bbabio.2020.148279
HAL: hal-02936602v1
Julie Szyttenholm, Florence Chaspoul, Marielle Bauzan, Anne-Lise Ducluzeau, Mahmoud Hajj Chehade, Fabien Pierrel, Yann Denis, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 1861 (10), 148252 -.
DOI: 10.1016/j.bbabio.2020.148252
HAL: hal-02896357v1
Simon Duval, Elbert Branscomb, Fabienne Trolard, Guilhem Bourrié, Olivier Grauby, Vasile Heresanu, Barbara Schoepp-Cothenet, Kilian Zuchan, Michael J. Russell, Wolfgang Nitschke.
On the why's and how's of clay minerals' importance in life's emergence.
Applied Clay Science, 2020, 195, 105737 -.
DOI: 10.1016/j.clay.2020.105737
HAL: hal-02936347v1
Ahmed Djeghader, Mélanie Rossotti, Saleh Abdulkarim, Frédéric Biaso, Guillaume Gerbaud, Wolfgang Nitschke, Barbara Schoepp-Cothenet, Tewfik Soulimane, Stephane Grimaldi.
Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase.
Chem. Commun., 2020, 56 (68), 9850 - 9853.
DOI: 10.1039/D0CC03634J
HAL: hal-02930401
2019
Simon Duval, Frauke Baymann, Barbara Schoepp-Cothenet, Fabienne Trolard, Guilhem Bourrié, Olivier Grauby, Elbert Branscomb, Michael J. Russell, Wolfgang Nitschke.
Fougerite: the not so simple progenitor of the first cells.
Interface Focus, 2019, 323 (6), 93 -.
DOI: 10.1098/rsfs.2019.0063
HAL: hal-02359667v1
2018
Frauke Baymann, Barbara Schoepp-Cothenet, Simon Duval, Marianne Guiral, Myriam Brugna, Carole Baffert, Michael J. Russell, Wolfgang Nitschke.
On the Natural History of Flavin-Based Electron Bifurcation.
Front. Microbiol., 2018, 8, 357 -.
DOI: 10.3389/fmicb.2018.01357
HAL: hal-01828959v1
2017
Frederic Melin, Barbara Schoepp-Cothenet, Saleh Abdulkarim, Mohamed R. Noor, Tewfik Soulimane, Petra Hellwig.
Electrochemical study of an electron shuttle diheme protein: The cytochrome c from T. thermophilus.
Inorganica Chimica Acta, 2017, 468, 252 - 259.
DOI: 10.1016/j.ica.2017.05.009
HAL: hal-01598382v1
Cameron Watson, Dimitri Niks, Russ Hille, Marta Vieira, Barbara Schoepp-Cothenet, Alexandra T. Marques, Maria João Romão, Teresa Santos-Silva, Joanne M. Santini.
Electron transfer through arsenite oxidase: Insights into Rieske interaction with cytochrome c.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2017, 1858 (10), 865 - 872.
DOI: 10.1016/j.bbabio.2017.08.003
HAL: hal-01585207v1
2016
Rodrigo Arias-Cartin, Pierre Ceccaldi, Barbara Schoepp-Cothenet, Klaudia Frick, Jean-Michel Blanc, Bruno Guigliarelli, Anne Walburger, Stéphane Grimaldi, Thorsten Friedrich, Véronique Receveur-Brechot, Axel Magalon.
Redox cofactors insertion in prokaryotic molybdoenzymes occurs via a conserved folding mechanism.
Sci Rep, 2016, 12 (1), 543.
DOI: 10.1038/srep37743
HAL: hal-01415067v1
Simon Duval, Joanne M. Santini, David Lemaire, Florence Chaspoul, Michael J. Russell, Stephane Grimaldi, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
The H-bond network surrounding the pyranopterins modulates redox cooperativity in the molybdenum- bis PGD cofactor in arsenite oxidase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (9), 1353 - 1362.
DOI: 10.1016/j.bbabio.2016.05.003
HAL: hal-01413292v1
Axel Magalon, Pierre Ceccaldi, Barbara Schoepp-Cothenet.
The Prokaryotic Mo/W-bisPGD Enzymes Family.
Molybdenum and Tungsten Enzymes : Biochemistry, 2016, 143 - 191.
DOI: 10.1039/9781782623915-00143
HAL: hal-01435336v1
2014
Marie Catherine Sforna, Pascal Philippot, Andrea Somogyi, Mark A. van Zuilen, Kadda Medjoubi, Barbara Schoepp-Cothenet, Wolfgang Nitschke, Pieter T. Visscher.
Evidence for arsenic metabolism and cycling by microorganisms 2.7 billion years ago.
Nature Geosci, 2014, 16 (11), 523 - 815.
DOI: 10.1038/ngeo2276
HAL: hal-01494482v1
Anne-Lise Ducluzeau, Barbara Schoepp-Cothenet, Robert van Lis, Frauke Baymann, Michael J. Russell, Wolfgang Nitschke.
The evolution of respiratory O
2
/NO reductases: an out-of-the-phylogenetic-box perspective.
J. R. Soc. Interface, 2014, 265 (98), 11 535 -.
DOI: 10.1098/rsif.2014.0196
HAL: hal-01494539v1
Anne-Lise Ducluzeau, Barbara Schoepp-Cothenet, Frauke Baymann, Michael J. Russell, Wolfgang Nitschke.
Free energy conversion in the LUCA: Quo vadis?.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (7), 982 - 988.
DOI: 10.1016/j.bbabio.2013.12.005
HAL: hal-01493447v1
2013
F. ten Brink, B. Schoepp-Cothenet, R. van Lis, W. Nitschke, F. Baymann.
Multiple Rieske/cytb complexes in a single organism.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (11-12), 1392 - 1406.
DOI: 10.1016/j.bbabio.2013.03.003
HAL: hal-01607947v1
Thomas P. Warelow, Muse Oke, Barbara Schoepp-Cothenet, Jan U. Dahl, Nicole Bruselat, Ganesh N. Sivalingam, Silke Leimkühler, Konstantinos Thalassinos, Ulrike Kappler, James H. Naismith, Joanne M. Santini.
The Respiratory Arsenite Oxidase: Structure and the Role of Residues Surrounding the Rieske Cluster.
PLoS ONE, 2013, 8 (8), e72535 -.
DOI: 10.1371/journal.pone.0072535
Stéphane Grimaldi, Barbara Schoepp-Cothenet, Pierre Ceccaldi, Bruno Guigliarelli, Axel Magalon.
The prokaryotic Mo/W-bisPGD enzymes family: A catalytic workhorse in bioenergetic.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (8-9), 1048 - 1085.
DOI: 10.1016/j.bbabio.2013.01.011
HAL: hal-03549630
Robert van Lis, Wolfgang Nitschke, Simon Duval, Barbara Schoepp-Cothenet.
Arsenics as bioenergetic substrates.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (2), 176 - 188.
DOI: 10.1016/j.bbabio.2012.08.007
HAL: hal-01601687v1
Barbara Schoepp-Cothenet, Robert van Lis, Ariane Atteia, Frauke Baymann, Line Capowiez, Anne-Lise Ducluzeau, Simon Duval, Felix ten Brink, Michael J. Russell, Wolfgang Nitschke.
On the universal core of bioenergetics.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (2), 79 - 93.
DOI: 10.1016/j.bbabio.2012.09.005
HAL: hal-01606377v1
2012
Robert van Lis, Wolfgang Nitschke, Thomas P. Warelow, Line Capowiez, Joanne M. Santini, Barbara Schoepp-Cothenet.
Heterologously expressed arsenite oxidase: A system to study biogenesis and structure/function relationships of the enzyme family.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012, 1817 (9), 1701 - 1708.
DOI: 10.1016/j.bbabio.2012.06.001
HAL: hal-01601795v1
Barbara Schoepp-Cothenet, Robert van Lis, Pascal Philippot, Axel Magalon, Michael J. Russell, Wolfgang Nitschke.
The ineluctable requirement for the trans-iron elements molybdenum and/or tungsten in the origin of life.
Sci Rep, 2012, 18 (1), 2714 -.
DOI: 10.1038/srep00263
HAL: hal-01608654v1
Frauke Baymann, Barbara Schoepp-Cothenet, Evelyne Lebrun, Robert van Lis, Wolfgang Nitschke.
Phylogeny of Rieske/cytb Complexes with a Special Focus on the Haloarchaeal Enzymes.
2012, 392 (8), 677 - 841.
DOI: 10.1093/gbe/evs056
HAL: hal-01607945v1
2011
B. Schoepp-Cothenet, W. Nitschke, L. M. Barge, A. Ponce, M. J. Russell, A. I. Tsapin.
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus".
Science, 2011, 323 (5914), 583c - 1149.
DOI: 10.1126/science.1201438
2010
Aurélie Lieutaud, Robert van Lis, Simon Duval, Line Capowiez, Daniel Muller, Régine Lebrun, Sabrina Lignon, Marie-Laure Fardeau, Marie-Claire Lett, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Arsenite Oxidase from
Ralstonia
sp. 22.
J. Biol. Chem., 2010, 35 (27), 3302 - 20441.
DOI: 10.1074/jbc.M110.113761
HAL: hal-01602032v1
Simon Duval, Joanne M. Santini, Wolfgang Nitschke, Russ Hille, Barbara Schoepp-Cothenet.
The Small Subunit AroB of Arsenite Oxidase.
J. Biol. Chem., 2010, Vol. I (27), 945 - 20451.
DOI: 10.1074/jbc.M110.113811
W. Nitschke, R. van Lis, B. Schoepp-Cothenet, F. Baymann.
The “green” phylogenetic clade of Rieske/cytb complexes.
Photosynth Res, 2010, 128 (2-3), 14246 - 355.
DOI: 10.1007/s11120-010-9532-1
HAL: hal-01607249v1
2009
B. Schoepp-Cothenet, C. Lieutaud, F. Baymann, A. Vermeglio, T. Friedrich, D. M. Kramer, W. Nitschke.
Menaquinone as pool quinone in a purple bacterium.
Proceedings of the National Academy of Sciences, 2009, 5 (3), 151 - 8554.
DOI: 10.1073/pnas.0813173106
B. Schoepp-Cothenet, S. Duval, J. M. Santini, W. Nitschke.
Comment on "Arsenic (III) Fuels Anoxygenic Photosynthesis in Hot Spring Biofilms from Mono Lake, California".
Science, 2009, 8 (5621), 206 - 583c.
DOI: 10.1126/science.1164967
Anne-Lise Ducluzeau, Robert van Lis, Simon Duval, Barbara Schoepp-Cothenet, Michael J. Russell, Wolfgang Nitschke.
Was nitric oxide the first deep electron sink?.
Trends in Biochemical Sciences, 2009, 34 (1), 9 - 15.
DOI: 10.1016/j.tibs.2008.10.005
HAL: hal-01607251v1
2008
Simon Duval, Anne-Lise Ducluzeau, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Enzyme phylogenies as markers for the oxidation state of the environment: The case of respiratory arsenate reductase and related enzymes.
BMC Evolutionary Biology, 2008, 8 (1), 206 -.
DOI: 10.1186/1471-2148-8-206
2007
Daniel Muller, Claudine Médigue, Sandrine Koechler, Valérie Barbe, Mohamed Barakat, Emmanuel Talla, Violaine Bonnefoy, Evelyne Krin, Florence Arsène-Ploetze, Christine Carapito, Michael Chandler, Benoît Cournoyer, Stéphane Cruveiller, Caroline Dossat, Sim.
A Tale of Two Oxidation States: Bacterial Colonization of Arsenic-Rich Environments.
PLoS Genet, 2007, 73 (4), 1917 -.
DOI: 10.1371/journal.pgen.0030053
HAL: hal-00340034v1
2006
Gérard Lipowski, Ursula Liebl, Bruno Guigliarelli, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Conformation of the c 552 : aa 3 electron transfer complex in Paracoccus denitrificans studied by EPR on oriented samples.
2006, 580 (25), 5988 - 5992.
DOI: 10.1016/j.febslet.2006.09.038
HAL: hal-00824462v1
Evelyne Lebrun, Joanne M. Santini, Myriam Brugna, Anne-Lise Ducluzeau, Soufian Ouchane, Barbara Schoepp-Cothenet, Frauke Baymann, Wolfgang Nitschke.
The Rieske Protein: A Case Study on the Pitfalls of Multiple Sequence Alignments and Phylogenetic Reconstruction.
2006, 23 (6), 1180 - 1191.
DOI: 10.1093/molbev/msk010
HAL: hal-00132690v1
2005
C. Lieutaud, J. Alric, M. Bauzan, W. Nitschke, B. Schoepp-Cothenet.
Study of the high-potential iron sulfur protein in Halorhodospira halophila confirms that it is distinct from cytochrome c as electron carrier.
Proceedings of the National Academy of Sciences, 2005, 1229 (2), 81 - 3265.
DOI: 10.1073/pnas.0407768102
HAL: hal-00339973v1
2004
Jean Alric, Yusuke Tsukatani, Makoto Yoshida, Katsumi Matsuura, Keizo Shimada, Rainer Hienerwadel, Barbara Schoepp-Cothenet, Wolfgang Nitschke, Kenji V. P. Nagashima, André Verméglio.
Structural and Functional Characterization of the Unusual Triheme Cytochrome Bound to the Reaction Center of
Rhodovulum sulfidophilum.
J. Biol. Chem., 2004, 108 (25), 1163 - 26097.
DOI: 10.1074/jbc.M400361200
2003
Michael Schütz, Barbara Schoepp-Cothenet, Elisabeth Lojou, Mireille Woodstra, Doris Lexa, Pascale Tron, Alain Dolla, Marie-Claire Durand, Karl Otto Stetter, Frauke Baymann.
The Naphthoquinol Oxidizing Cytochrome
bc
1
Complex of the Hyperthermophilic Knallgasbacterium
Aquifex aeolicus
: Properties and Phylogenetic Relationships
†.
Biochemistry, 2003, 42 (36), 10800 - 10808.
DOI: 10.1021/bi034452a
Catherine Duport, Barbara Schoepp, Elise Chatelain, Roberto Spagnoli, Bruno Dumas, Denis Pompon.
Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast.
Eur J Biochem, 2003, 6 (7), 521 - 1514.
DOI: 10.1046/j.1432-1033.2003.03516.x
Frauke Baymann, Nicola L Barlow, Corinne Aubert, Barbara Schoepp-Cothenet, Gisele Leroy, Fraser A Armstrong.
Voltammetry of a ‘protein on a rope’.
2003, 9 (1-3), 48 - 94.
DOI: 10.1016/S0014-5793(03)00206-0
Clément Lieutaud, Wolfgang Nitschke, André Verméglio, Pierre Parot, Barbara Schoepp-Cothenet.
HiPIP in Rubrivivax gelatinosus is firmly associated to the membrane in a conformation efficient for electron transfer towards the photosynthetic reaction centre.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2003, 1557, 83 - 90.
DOI: 10.1016/S0005-2728(02)00397-3
2001
Frauke Baymann, Pascale Tron, Barbara Schoepp-Cothenet, Corinne Aubert, Pierre Bianco, Karl-Otto Stetter, Wolfgang Nitschke, Michael Schütz.
Cytochromes
c
555
from the Hyperthermophilic Bacterium
Aquifex
aeolicus
(VF5). 1. Characterization of Two Highly Homologous, Soluble and Membranous, Cytochromes.
Biochemistry, 2001, 40 (45), 13681 - 13689.
DOI: 10.1021/bi011201y
Barbara Schoepp-Cothenet, Michael Schütz, Frauke Baymann, Myriam Brugna, Wolfgang Nitschke, Hannu Myllykallio, Christian Schmidt.
The membrane-extrinsic domain of cytochrome b 558/566 from the Archaeon Sulfolobus acidocaldarius performs pivoting movements with respect to the membrane surface.
2001, 16 (3), 544 - 376.
DOI: 10.1016/S0014-5793(00)02357-7
1999
Barbara Schoepp, Myriam Brugna, Astrid Riedel, Wolfgang Nitschke, David M. Kramer.
The Q o -site inhibitor DBMIB favours the proximal position of the chloroplast Rieske protein and induces a p K -shift of the redox-linked proton.
1999, 112 (3), 535 - 250.
DOI: 10.1016/S0014-5793(99)00511-6
1997
David M. Kramer, Barbara Schoepp, Ursula Liebl, Wolfgang Nitschke.
Cyclic Electron Transfer in
Heliobacillus mobilis
Involving a Menaquinol-Oxidizing Cytochrome
bc
Complex and an RCI-Type Reaction Center
†.
Biochemistry, 1997, 36 (14), 4203 - 4211.
DOI: 10.1021/bi962241i