Barbara SCHOEPP-COTHENET

member photo

Directrice de Recherche, CNRS

+33 4 91 16 40 97

Chef d'équipe / Group leader: BIP 09


Sujet de Recherche :
Les substrats énergétiques utilisés par les procaryotes sont extrêmement divers et comprennent même des substances toxiques pour la plupart des organismes. L’arsenic apparait ainsi de plus en plus clairement comme un substrat microbien bioénergétique majeur, utilisé dès l’archéen. Notre objectif de recherche est non seulement de comprendre la place du métabolisme de l’Arsenic dans l’évolution globale des processus énergétiques et de la vie sur terre, mais également de décortiquer les mystères encore nombreux des mécanismes moléculaires du fonctionnement des enzymes impliquées dans le métabolisme énergétique de l’Arsenic : arsénite oxydase Aio, arsénite oxydase alternative Arx et arséniate reductase Arr. Ces questions sont abordées selon la spécificité de notre équipe BIP09, c’est-à-dire l’approche phylogénétique conjointe à l’expérimentation biochimique (enzymologie, purifications), biophysique (spectroscopie optique et RPE) et microbiologique. Celles-ci nous amènent à étudier 1) les déterminants moléculaires des propriétés d’autres enzymes à Molybdène (Mo) que celles impliquées dans le métabolisme de l’Arsenic, comme la TMAO réductase TorA, la Sulfite oxydase Soe 2) la phylogénie d’enzymes autres que celles du métabolisme de l’Arsenic, en lien avec celui-ci, par exemple les enzymes du métabolisme du soufre ou du nitrate.


Publications
ORCID id: 0000-0002-6820-4478

2020

Simon Duval, Elbert Branscomb, Fabienne Trolard, Guilhem Bourrié, Olivier Grauby, Vasile Heresanu, Barbara Schoepp-Cothenet, Kilian Zuchan, Michael J. Russell, Wolfgang Nitschke.
On the why's and how's of clay minerals' importance in life's emergence.
Applied Clay Science, 2020, 195, 105737 -. DOI: 10.1016/j.clay.2020.105737

Julie Szyttenholm, Florence Chaspoul, Marielle Bauzan, Anne-Lise Ducluzeau, Mahmoud Hajj Chehade, Fabien Pierrel, Yann Denis, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 1861 (10), 148252 -. DOI: 10.1016/j.bbabio.2020.148252



2019

Simon Duval, Frauke Baymann, Barbara Schoepp-Cothenet, Fabienne Trolard, Guilhem Bourrié, Olivier Grauby, Elbert Branscomb, Michael J. Russell, Wolfgang Nitschke.
Fougerite: the not so simple progenitor of the first cells.
Interface Focus, 2019, 323 (6), 93 -. DOI: 10.1098/rsfs.2019.0063 HAL: hal-02359667v1



2018

Frauke Baymann, Barbara Schoepp-Cothenet, Simon Duval, Marianne Guiral, Myriam Brugna, Carole Baffert, Michael J. Russell, Wolfgang Nitschke.
On the Natural History of Flavin-Based Electron Bifurcation.
Front. Microbiol., 2018, 8, 357 -. DOI: 10.3389/fmicb.2018.01357 HAL: hal-01828959v1



2017

Frederic Melin, Barbara Schoepp-Cothenet, Saleh Abdulkarim, Mohamed R. Noor, Tewfik Soulimane, Petra Hellwig.
Electrochemical study of an electron shuttle diheme protein: The cytochrome c from T. thermophilus.
Inorganica Chimica Acta, 2017, 468, 252 - 259. DOI: 10.1016/j.ica.2017.05.009 HAL: hal-01598382v1

Cameron Watson, Dimitri Niks, Russ Hille, Marta Vieira, Barbara Schoepp-Cothenet, Alexandra T. Marques, Maria João Romão, Teresa Santos-Silva, Joanne M. Santini.
Electron transfer through arsenite oxidase: Insights into Rieske interaction with cytochrome c.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2017, 1858 (10), 865 - 872. DOI: 10.1016/j.bbabio.2017.08.003 HAL: hal-01585207v1



2016

Axel Magalon, Pierre Ceccaldi, Barbara Schoepp-Cothenet.
Metallobiology.
2016, 143 - 191. DOI: 10.1039/9781782623915-00143 HAL: hal-01435336v1

Rodrigo Arias-Cartin, Pierre Ceccaldi, Barbara Schoepp-Cothenet, Klaudia Frick, Jean-Michel Blanc, Bruno Guigliarelli, Anne Walburger, Stéphane Grimaldi, Thorsten Friedrich, Véronique Receveur-Brechot, Axel Magalon.
Redox cofactors insertion in prokaryotic molybdoenzymes occurs via a conserved folding mechanism.
Sci Rep, 2016, 12 (1), 543 -. DOI: 10.1038/srep37743 HAL: hal-01415067v1

Simon Duval, Joanne M. Santini, David Lemaire, Florence Chaspoul, Michael J. Russell, Stephane Grimaldi, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
The H-bond network surrounding the pyranopterins modulates redox cooperativity in the molybdenum- bis PGD cofactor in arsenite oxidase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (9), 1353 - 1362. DOI: 10.1016/j.bbabio.2016.05.003 HAL: hal-01413292v1



2014

Marie Catherine Sforna, Pascal Philippot, Andrea Somogyi, Mark A. van Zuilen, Kadda Medjoubi, Barbara Schoepp-Cothenet, Wolfgang Nitschke, Pieter T. Visscher.
Evidence for arsenic metabolism and cycling by microorganisms 2.7 billion years ago.
Nature Geosci, 2014, 16 (11), 523 - 815. DOI: 10.1038/ngeo2276 HAL: hal-01494482v1

Anne-Lise Ducluzeau, Barbara Schoepp-Cothenet, Frauke Baymann, Michael J. Russell, Wolfgang Nitschke.
Free energy conversion in the LUCA: Quo vadis?.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (7), 982 - 988. DOI: 10.1016/j.bbabio.2013.12.005 HAL: hal-01493447v1

Anne-Lise Ducluzeau, Barbara Schoepp-Cothenet, Robert van Lis, Frauke Baymann, Michael J. Russell, Wolfgang Nitschke.
The evolution of respiratory O 2 /NO reductases: an out-of-the-phylogenetic-box perspective.
J. R. Soc. Interface, 2014, 265 (98), 11 535 -. DOI: 10.1098/rsif.2014.0196 HAL: hal-01494539v1



2013

Robert van Lis, Wolfgang Nitschke, Simon Duval, Barbara Schoepp-Cothenet.
Arsenics as bioenergetic substrates.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (2), 176 - 188. DOI: 10.1016/j.bbabio.2012.08.007 HAL: hal-01601687v1

F. ten Brink, B. Schoepp-Cothenet, R. van Lis, W. Nitschke, F. Baymann.
Multiple Rieske/cytb complexes in a single organism.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (11-12), 1392 - 1406. DOI: 10.1016/j.bbabio.2013.03.003 HAL: hal-01607947v1

Barbara Schoepp-Cothenet, Robert van Lis, Ariane Atteia, Frauke Baymann, Line Capowiez, Anne-Lise Ducluzeau, Simon Duval, Felix ten Brink, Michael J. Russell, Wolfgang Nitschke.
On the universal core of bioenergetics.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (2), 79 - 93. DOI: 10.1016/j.bbabio.2012.09.005 HAL: hal-01606377v1

Stéphane Grimaldi, Barbara Schoepp-Cothenet, Pierre Ceccaldi, Bruno Guigliarelli, Axel Magalon.
The prokaryotic Mo/W-bisPGD enzymes family: A catalytic workhorse in bioenergetic.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (8-9), 1048 - 1085. DOI: 10.1016/j.bbabio.2013.01.011

Thomas P. Warelow, Muse Oke, Barbara Schoepp-Cothenet, Jan U. Dahl, Nicole Bruselat, Ganesh N. Sivalingam, Silke Leimkühler, Konstantinos Thalassinos, Ulrike Kappler, James H. Naismith, Joanne M. Santini.
The Respiratory Arsenite Oxidase: Structure and the Role of Residues Surrounding the Rieske Cluster.
PLoS ONE, 2013, 8 (8), e72535 -. DOI: 10.1371/journal.pone.0072535



2012

Robert van Lis, Wolfgang Nitschke, Thomas P. Warelow, Line Capowiez, Joanne M. Santini, Barbara Schoepp-Cothenet.
Heterologously expressed arsenite oxidase: A system to study biogenesis and structure/function relationships of the enzyme family.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012, 1817 (9), 1701 - 1708. DOI: 10.1016/j.bbabio.2012.06.001 HAL: hal-01601795v1

Frauke Baymann, Barbara Schoepp-Cothenet, Evelyne Lebrun, Robert van Lis, Wolfgang Nitschke.
Phylogeny of Rieske/cytb Complexes with a Special Focus on the Haloarchaeal Enzymes.
2012, 392 (8), 677 - 841. DOI: 10.1093/gbe/evs056 HAL: hal-01607945v1

Barbara Schoepp-Cothenet, Robert van Lis, Pascal Philippot, Axel Magalon, Michael J. Russell, Wolfgang Nitschke.
The ineluctable requirement for the trans-iron elements molybdenum and/or tungsten in the origin of life.
Sci Rep, 2012, 18 (1), 2714 -. DOI: 10.1038/srep00263 HAL: hal-01608654v1



2011

B. Schoepp-Cothenet, W. Nitschke, L. M. Barge, A. Ponce, M. J. Russell, A. I. Tsapin.
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus".
Science, 2011, 323 (5914), 583c - 1149. DOI: 10.1126/science.1201438



2010

Aurélie Lieutaud, Robert van Lis, Simon Duval, Line Capowiez, Daniel Muller, Régine Lebrun, Sabrina Lignon, Marie-Laure Fardeau, Marie-Claire Lett, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Arsenite Oxidase from Ralstonia sp. 22.
J. Biol. Chem., 2010, 35 (27), 3302 - 20441. DOI: 10.1074/jbc.M110.113761 HAL: hal-01602032v1

Simon Duval, Joanne M. Santini, Wolfgang Nitschke, Russ Hille, Barbara Schoepp-Cothenet.
The Small Subunit AroB of Arsenite Oxidase.
J. Biol. Chem., 2010, Vol. I (27), 945 - 20451. DOI: 10.1074/jbc.M110.113811

W. Nitschke, R. van Lis, B. Schoepp-Cothenet, F. Baymann.
The “green” phylogenetic clade of Rieske/cytb complexes.
Photosynth Res, 2010, 128 (2-3), 14246 - 355. DOI: 10.1007/s11120-010-9532-1 HAL: hal-01607249v1



2009

B. Schoepp-Cothenet, S. Duval, J. M. Santini, W. Nitschke.
Comment on "Arsenic (III) Fuels Anoxygenic Photosynthesis in Hot Spring Biofilms from Mono Lake, California".
Science, 2009, 8 (5621), 206 - 583c. DOI: 10.1126/science.1164967

B. Schoepp-Cothenet, C. Lieutaud, F. Baymann, A. Vermeglio, T. Friedrich, D. M. Kramer, W. Nitschke.
Menaquinone as pool quinone in a purple bacterium.
Proceedings of the National Academy of Sciences, 2009, 5 (3), 151 - 8554. DOI: 10.1073/pnas.0813173106

Anne-Lise Ducluzeau, Robert van Lis, Simon Duval, Barbara Schoepp-Cothenet, Michael J. Russell, Wolfgang Nitschke.
Was nitric oxide the first deep electron sink?.
Trends in Biochemical Sciences, 2009, 34 (1), 9 - 15. DOI: 10.1016/j.tibs.2008.10.005 HAL: hal-01607251v1



2008

Simon Duval, Anne-Lise Ducluzeau, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Enzyme phylogenies as markers for the oxidation state of the environment: The case of respiratory arsenate reductase and related enzymes.
BMC Evolutionary Biology, 2008, 8 (1), 206 -. DOI: 10.1186/1471-2148-8-206



2007

Daniel Muller, Claudine Médigue, Sandrine Koechler, Valérie Barbe, Mohamed Barakat, Emmanuel Talla, Violaine Bonnefoy, Evelyne Krin, Florence Arsène-Ploetze, Christine Carapito, Michael Chandler, Benoît Cournoyer, Stéphane Cruveiller, Caroline Dossat, Sim.
A Tale of Two Oxidation States: Bacterial Colonization of Arsenic-Rich Environments.
PLoS Genet, 2007, 73 (4), 1917 -. DOI: 10.1371/journal.pgen.0030053 HAL: hal-00340034v1



2006

Gérard Lipowski, Ursula Liebl, Bruno Guigliarelli, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Conformation of the c 552 : aa 3 electron transfer complex in Paracoccus denitrificans studied by EPR on oriented samples.
2006, 409 (25), 128 - 5992. DOI: 10.1016/j.febslet.2006.09.038 HAL: hal-00824462v1

Evelyne Lebrun, Joanne M. Santini, Myriam Brugna, Anne-Lise Ducluzeau, Soufian Ouchane, Barbara Schoepp-Cothenet, Frauke Baymann, Wolfgang Nitschke.
The Rieske Protein: A Case Study on the Pitfalls of Multiple Sequence Alignments and Phylogenetic Reconstruction.
2006, 23 (6), 1180 - 1191. DOI: 10.1093/molbev/msk010 HAL: hal-00132690v1



2005

C. Lieutaud, J. Alric, M. Bauzan, W. Nitschke, B. Schoepp-Cothenet.
Study of the high-potential iron sulfur protein in Halorhodospira halophila confirms that it is distinct from cytochrome c as electron carrier.
Proceedings of the National Academy of Sciences, 2005, 1229 (2), 81 - 3265. DOI: 10.1073/pnas.0407768102 HAL: hal-00339973v1



2004

Jean Alric, Yusuke Tsukatani, Makoto Yoshida, Katsumi Matsuura, Keizo Shimada, Rainer Hienerwadel, Barbara Schoepp-Cothenet, Wolfgang Nitschke, Kenji V. P. Nagashima, André Verméglio.
Structural and Functional Characterization of the Unusual Triheme Cytochrome Bound to the Reaction Center of Rhodovulum sulfidophilum.
J. Biol. Chem., 2004, 108 (25), 1163 - 26097. DOI: 10.1074/jbc.M400361200



2003

Catherine Duport, Barbara Schoepp, Elise Chatelain, Roberto Spagnoli, Bruno Dumas, Denis Pompon.
Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast.
Eur J Biochem, 2003, 6 (7), 521 - 1514. DOI: 10.1046/j.1432-1033.2003.03516.x

Clément Lieutaud, Wolfgang Nitschke, André Verméglio, Pierre Parot, Barbara Schoepp-Cothenet.
HiPIP in Rubrivivax gelatinosus is firmly associated to the membrane in a conformation efficient for electron transfer towards the photosynthetic reaction centre.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2003, 1557, 83 - 90. DOI: 10.1016/S0005-2728(02)00397-3

Michael Schütz, Barbara Schoepp-Cothenet, Elisabeth Lojou, Mireille Woodstra, Doris Lexa, Pascale Tron, Alain Dolla, Marie-Claire Durand, Karl Otto Stetter, Frauke Baymann.
The Naphthoquinol Oxidizing Cytochrome bc 1 Complex of the Hyperthermophilic Knallgasbacterium Aquifex aeolicus : Properties and Phylogenetic Relationships †.
Biochemistry, 2003, 42 (36), 10800 - 10808. DOI: 10.1021/bi034452a

Frauke Baymann, Nicola L Barlow, Corinne Aubert, Barbara Schoepp-Cothenet, Gisele Leroy, Fraser A Armstrong.
Voltammetry of a ‘protein on a rope’.
2003, 9 (1-3), 48 - 94. DOI: 10.1016/S0014-5793(03)00206-0



2001

Frauke Baymann, Pascale Tron, Barbara Schoepp-Cothenet, Corinne Aubert, Pierre Bianco, Karl-Otto Stetter, Wolfgang Nitschke, Michael Schütz.
Cytochromes c 555 from the Hyperthermophilic Bacterium Aquifex aeolicus (VF5). 1. Characterization of Two Highly Homologous, Soluble and Membranous, Cytochromes.
Biochemistry, 2001, 40 (45), 13681 - 13689. DOI: 10.1021/bi011201y

Barbara Schoepp-Cothenet, Michael Schütz, Frauke Baymann, Myriam Brugna, Wolfgang Nitschke, Hannu Myllykallio, Christian Schmidt.
The membrane-extrinsic domain of cytochrome b 558/566 from the Archaeon Sulfolobus acidocaldarius performs pivoting movements with respect to the membrane surface.
2001, 16 (3), 544 - 376. DOI: 10.1016/S0014-5793(00)02357-7



1999

Barbara Schoepp, Myriam Brugna, Astrid Riedel, Wolfgang Nitschke, David M. Kramer.
The Q o -site inhibitor DBMIB favours the proximal position of the chloroplast Rieske protein and induces a p K -shift of the redox-linked proton.
1999, 112 (3), 535 - 250. DOI: 10.1016/S0014-5793(99)00511-6



1997

David M. Kramer, Barbara Schoepp, Ursula Liebl, Wolfgang Nitschke.
Cyclic Electron Transfer in Heliobacillus mobilis Involving a Menaquinol-Oxidizing Cytochrome bc Complex and an RCI-Type Reaction Center †.
Biochemistry, 1997, 36 (14), 4203 - 4211. DOI: 10.1021/bi962241i