Bruno GUIGLIARELLI

Professeur, HDR, Aix-Marseille Université

+33 4 91 16 45 67

Equipe / Group: BIP 07

Publications

2024

Maxime Bizet, Deborah Byrne, Frédéric Biaso, Guillaume Gerbaud, Emilien Etienne, Giuseppina Briola, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Tamas Kalai, Gilles Truan, Marlene Martinho.
Structural insights into the semiquinone form of human Cytochrome P450 reductase by DEER distance measurements between a native flavin and a spin labelled non‐canonical amino acid.
Chemistry Eur J, 2024. DOI: 10.1002/chem.202304307 HAL: hal-04426634

Ana Rita Oliveira, Cristiano Mota, Guilherme Vilela-Alves, Rita Rebelo Manuel, Neide Pedrosa, Vincent Fourmond, Kateryna Klymanska, Christophe Léger, Bruno Guigliarelli, Maria João Romão, Inês A. Cardoso Pereira.
An allosteric redox switch involved in oxygen protection in a CO2 reductase.
Nat Chem Biol, 2024, 20 (1), 111-119. DOI: 10.1038/s41589-023-01484-2 HAL: hal-04383526 (dataset available here)

2023

Annalisa Pierro, Ketty Concetta Tamburrini, Hugo Leguenno, Guillaume Gerbaud, Emilien Etienne, Bruno Guigliarelli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR.
iScience, 2023, 26 (10), 107855. DOI: 10.1016/j.isci.2023.107855 HAL: hal-04233485v1

Pierre Nabokoff, Guillaume Brulay, Cyrielle Dol, Guillaume Gerbaud, Bruno Guigliarelli, Emilien Etienne, Emily Bloch, Fabio Ziarelli, Eric Besson, Stéphane Gastaldi.
Synthesis and Characterization of Face-to-Face Arylsulfanyl and Phenoxyl Radicals Embedded in Mesoporous Silicas.
J. Phys. Chem. C, 2023, 127 (20), 9699 - 9706. DOI: 10.1021/acs.jpcc.3c01550 HAL: hal-04114864v1

Laura Prioretti, Giulia D'Ermo, Pascale Infossi, Arlette Kpebe, Régine Lebrun, Marielle Bauzan, Elisabeth Lojou, Bruno Guigliarelli, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
Carbon Fixation in the Chemolithoautotrophic Bacterium Aquifex aeolicus Involves Two Low-Potential Ferredoxins as Partners of the PFOR and OGOR Enzymes.
Life, 2023, 13 (3), 627. DOI: 10.3390/life13030627 HAL: hal-04071170v1

Alejandro Villalta, Batoul Srour, Audrey Lartigue, Martin Clémancey, Deborah Byrne, Florence Chaspoul, Antoine Loquet, Bruno Guigliarelli, Geneviève Blondin, Chantal Abergel, Bénédicte Burlat.
Evidence for [2Fe-2S] 2+ and Linear [3Fe-4S] 1+ Clusters in a Unique Family of Glycine/Cysteine-Rich Fe-S Proteins from Megavirinae Giant Viruses.
J. Am. Chem. Soc., 2023, 145 (5), 2733–2738. DOI: 10.1021/jacs.2c10484 HAL: hal-03967087v1

2022

Annalisa Pierro, Alessio Bonucci, Davide Normanno, Mireille Ansaldi, Eric Pilet, Olivier Ouari, Bruno Guigliarelli, Emilien Etienne, Guillaume Gerbaud, Axel Magalon, Valérie Belle, Elisabetta Mileo.
Probing Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide‐Based EPR Spectroscopy.
Chemistry A European J, 2022. DOI: 10.1002/chem.202202249 HAL: hal-03836431

Batoul Srour, Sylvain Gervason, Maren Hellen Hoock, Beata Monfort, Kristian Want, Djabir Larkem, Nadine Trabelsi, Gautier Landrot, Andrea Zitolo, Emiliano Fonda, Emilien Etienne, Guillaume Gerbaud, Christina Sophia Müller, Jonathan Oltmanns, Jesse B. Gordon, Vishal Yadav, Malgorzata Kleczewska, Marcin Jelen, Michel B. Toledano, Rafal Dutkiewicz, David P. Goldberg, Volker Schünemann, Bruno Guigliarelli, Bénédicte Burlat, Christina Sizun, Benoit D’Autréaux.
Iron Insertion at the Assembly Site of the ISCU Scaffold Protein Is a Conserved Process Initiating Fe–S Cluster Biosynthesis.
J. Am. Chem. Soc., 2022. DOI: 10.1021/jacs.2c06338 HAL: hal-03837292v1

Maryam Seif-Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
Comparative analysis of the mena- and demethylmena-(semi)quinone binding mode to the quinol oxidation site of E. coli nitrate reductase A.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, 148750. DOI: 10.1016/j.bbabio.2022.148750

Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle.
EPR-MRS site: EPR spectroscopy for the study of biomolecules.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, Supplement, 29. DOI: 10.1016/j.bbabio.2022.148685 HAL: hal-03805551v1

Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle.
MOSBRI- A New European Research Infrastructure for Molecular Biophysics.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, Supplement, 28. DOI: 10.1016/j.bbabio.2022.148684 HAL: hal-03805560v1

Eric Pilet, Maryam Seif-Eddine, Farida Seduk, Julia Rendon, Bruno Guigliarelli, Stéphane Grimaldi, Axel Magalon.
New insights in quinone reactivity in the nitrate reductase A from E. coli.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, 148814. DOI: 10.1016/j.bbabio.2022.148814

Ana Rita Oliveira, Cristiano Mota, Kateryna Klymanska, Frédéric Biaso, Maria João Romão, Bruno Guigliarelli, Inês Cardoso Pereira.
Spectroscopic and Structural Characterization of Reduced Desulfovibrio vulgaris Hildenborough W-FdhAB Reveals Stable Metal Coordination during Catalysis.
ACS Chem. Biol., 2022, 17 (7), 1901 - 1909. DOI: 10.1021/acschembio.2c00336

2021

Sinan Al-Attar, Julia Rendon, Marlon Sidore, Jean-Pierre Duneau, Farida Seduk, Frédéric Biaso, Stéphane Grimaldi, Bruno Guigliarelli, Axel Magalon.
Gating of Substrate Access and Long-Range Proton Transfer in Escherichia coli Nitrate Reductase A: The Essential Role of a Remote Glutamate Residue.
ACS Catal., 2021, 14303 - 14318. DOI: 10.1021/acscatal.1c03988 HAL: hal-03511729v1

Rodrigo Arias-Cartin, Alexandre Uzel, Farida Seduk, Guillaume Gerbaud, Fabien Pierrel, Marianne Broc, Régine Lebrun, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi, Anne Walburger.
Identification and characterization of a non-canonical menaquinone-linked formate dehydrogenase.
Journal of Biological Chemistry, 2021, 101384. DOI: 10.1016/j.jbc.2021.101384 HAL: hal-03431446v1

Christelle Caux, Bruno Guigliarelli, Corinne Vivès, Frédéric Biaso, Marius Horeau, Hawra Hassoune, Isabelle Petit-Hartlein, Céline Juillan-Binard, Stephane Torelli, Franck Fieschi, Vincent Nivière.
Membrane-Bound Flavocytochrome MsrQ Is a Substrate of the Flavin Reductase Fre in Escherichia coli.
ACS Chem. Biol., 2021. DOI: 10.1021/acschembio.1c00613 HAL: hal-03320876v1

Umberto Contaldo, Bruno Guigliarelli, Julien Perard, Clara Rinaldi, Alan Le Goff, Christine Cavazza.
Efficient Electrochemical CO2/CO Interconversion by an Engineered Carbon Monoxide Dehydrogenase on a Gas-Diffusion Carbon Nanotube-Based Bioelectrode.
ACS Catal., 2021, 11 (9), 5808 - 5817. DOI: 10.1021/acscatal.0c05437 HAL: hal-03258521v1

Majid Haddad Momeni, Folmer Fredslund, Bastien Bissaro, Olanrewaju Raji, Thu V. Vuong, Sebastian Meier, Tine Sofie Nielsen, Vincent Lombard, Bruno Guigliarelli, Frédéric Biaso, Mireille Haon, Sacha Grisel, Bernard Henrissat, Ditte Hededam Welner, Emma R. Master, Jean-Guy Berrin, Maher Abou Hachem.
Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs.
Nat Commun, 2021, 12 (1). DOI: 10.1038/s41467-021-22372-0 HAL: hal-03234953v1

2020

Annalisa Pierro, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Stefano Ciurli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
Nickel and GTP Modulate Helicobacter pylori UreG Structural Flexibility.
Biomolecules, 2020, 10 (7), 1062. DOI: 10.3390/biom10071062 HAL: hal-02902916v1

Maryam Seif Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
1,2H hyperfine spectroscopy and DFT modeling unveil the demethylmenasemiquinone binding mode to E. coli nitrate reductase A (NarGHI).
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 1861 (8), 148203. DOI: 10.1016/j.bbabio.2020.148203 HAL: hal-02909846

Ana Rita Oliveira, Cristiano Mota, Cláudia Mourato, Renato M. Domingos, Marino F. A. Santos, Diana Gesto, Bruno Guigliarelli, Teresa Santos-Silva, Maria João Romão, Inês A. Cardoso Pereira.
Toward the Mechanistic Understanding of Enzymatic CO2 Reduction.
ACS Catal., 2020, 10 (6), 3844 - 3856. DOI: 10.1021/acscatal.0c00086 HAL: hal-02938569

Alessio Bonucci, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo.
In‐Cell EPR: Progress towards Structural Studies Inside Cells.
ChemBioChem, 2020, 21 (4), 451 - 460. DOI: 10.1002/cbic.201900291 HAL: hal-02180300v1

2019

Kamal Zeamari, Guillaume Gerbaud, Sandrine Grosse, Vincent Fourmond, Florence Chaspoul, Frédéric Biaso, Pascal Arnoux, Monique Sabaty, David Pignol, Bruno Guigliarelli, Bénédicte Burlat.
Tuning the redox properties of a [4Fe-4S] center to modulate the activity of Mo-bisPGD periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2019, 1860 (5), 402 - 413. DOI: 10.1016/j.bbabio.2019.01.003 HAL: hal-02106668v1

Cyrielle Dol, Guillaume Gerbaud, Bruno Guigliarelli, Emily Bloch, Stéphane Gastaldi, Eric Besson.
Modulating lifetimes and relaxation times of phenoxyl radicals through their incorporation into different hybrid nanostructures.
Phys. Chem. Chem. Phys., 2019, 172 (29), 573 - 16344. DOI: 10.1039/C9CP03052B HAL: hal-02177931v1

Nolwenn Le Breton, Sonia Longhi, Antal Rockenbauer, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle, Marlène Martinho.
Probing the dynamic properties of two sites simultaneously in a protein–protein interaction process: a SDSL-EPR study.
Phys. Chem. Chem. Phys., 2019, 21, 22584 - 22588. DOI: 10.1039/c9cp04660g HAL: hal-02341622v1

2018

Arlette Kpebe, Martino Benvenuti, Chloé Guendon, Amani Rebai, Victoria Fernandez, Sébastien Le Laz, Emilien Etienne, Bruno Guigliarelli, Gabriel García-Molina, Antonio L. de Lacey, Carole Baffert, Myriam Brugna.
A new mechanistic model for an O2-protected electron-bifurcating hydrogenase, Hnd from Desulfovibrio fructosovorans.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2018, 1859 (12), 1302 - 1312. DOI: 10.1016/j.bbabio.2018.09.364 HAL: hal-01928576v1

Marlène Martinho, Diane Allegro, Isabelle Huvent, Charlotte Chabaud, Emilien Etienne, Hervé Kovacic, Bruno Guigliarelli, Vincent Peyrot, Isabelle Landrieu, Valérie Belle, Pascale Barbier.
Two Tau binding sites on tubulin revealed by thiol-disulfide exchanges.
Sci Rep, 2018, 8:13846, 1 - 11. DOI: 10.1038/s41598-018-32096-9 HAL: hal-01883844v1

Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, David Guieysse, Laurence Girbal, Vincent Fourmond, Isabelle André, Bruno Guigliarelli, Christophe Léger, Philippe Soucaille, Isabelle Meynial-Salles.
Roles of the F-domain in [FeFe] hydrogenase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2018, 1859 (2), 69 - 77. DOI: 10.1016/j.bbabio.2017.08.010 HAL: cea-01950818

Ganesan Karthikeyan, Alessio Bonucci, Gilles Casano, Guillaume Gerbaud, Sébastien Abel, Virginie Thomé, Laurent Kodjabachian, Axel Magalon, Bruno Guigliarelli, Valérie Belle, Olivier Ouari, Elisabetta Mileo.
A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies.
Angew. Chem. Int. Ed., 2018, 57, 1366 - 1370. DOI: 10.1002/anie.201710184 HAL: hal-02000559v1

2017

Christoph Gmeiner, Daniel Klose, Elisabetta Mileo, Valérie Belle, Sylvain R. A. Marque, Georg Dorn, Frédéric H. T. Allain, Bruno Guigliarelli, Gunnar Jeschke, Maxim Yulikov.
Orthogonal Tyrosine and Cysteine Site-Directed Spin Labeling for Dipolar Pulse EPR Spectroscopy on Proteins.
J. Phys. Chem. Lett., 2017, 8 (19), 4852 - 4857. DOI: 10.1021/acs.jpclett.7b02220 HAL: hal-01596569v1

Maryam Seif Eddine, Frédéric Biaso, Rodrigo Arias‐Cartin, Eric Pilet, Julia Rendon, Sevdalina Lyubenova, Farida Seduk, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
Probing the Menasemiquinone Binding Mode to Nitrate Reductase A by Selective 2 H and 15 N Labeling, HYSCORE Spectroscopy, and DFT Modeling.
ChemPhysChem, 2017, 18 (19), 2704 - 2714. DOI: 10.1002/cphc.201700571 HAL: hal-01635739v1

Marta Palombo, Alessio Bonucci, Emilien Etienne, Stefano Ciurli, Vladimir N. Uversky, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo, Barbara Zambelli.
The relationship between folding and activity in UreG, an intrinsically disordered enzyme.
Sci Rep, 2017, 89 (1), 392. DOI: 10.1038/s41598-017-06330-9 HAL: hal-01585289v1

Julia Rendon, Frédéric Biaso, Pierre Ceccaldi, René Toci, Farida Seduk, Axel Magalon, Bruno Guigliarelli, Stéphane Grimaldi.
Elucidating the Structures of the Low- and High-pH Mo(V) Species in Respiratory Nitrate Reductase: A Combined EPR, 14,15 N HYSCORE, and DFT Study.
Inorg. Chem., 2017, 56 (8), 4422 - 4434. DOI: 10.1021/acs.inorgchem.6b03129 HAL: hal-01514650v1

Alda Lisa Concia, Maria Rosa Beccia, Maylis Orio, Francine Terra Ferre, Marciela Scarpellini, Frédéric Biaso, Bruno Guigliarelli, Marius Réglier, A. Jalila Simaan.
Copper Complexes as Bioinspired Models for Lytic Polysaccharide Monooxygenases.
Inorg. Chem., 2017, 56 (3), 1023 - 1026. DOI: 10.1021/acs.inorgchem.6b02165 HAL: hal-01440965v1

2016

Rodrigo Arias-Cartin, Pierre Ceccaldi, Barbara Schoepp-Cothenet, Klaudia Frick, Jean-Michel Blanc, Bruno Guigliarelli, Anne Walburger, Stéphane Grimaldi, Thorsten Friedrich, Véronique Receveur-Brechot, Axel Magalon.
Redox cofactors insertion in prokaryotic molybdoenzymes occurs via a conserved folding mechanism.
Sci Rep, 2016, 12 (1), 543. DOI: 10.1038/srep37743 HAL: hal-01415067v1

Sona Garajova, Yann Mathieu, Maria Rosa Beccia, Chloé Bennati-Granier, Frédéric Biaso, Mathieu Fanuel, David Ropartz, Bruno Guigliarelli, Eric Record, Hélène Rogniaux, Bernard Henrissat, Jean-Guy Berrin.
Single-domain flavoenzymes trigger lytic polysaccharide monooxygenases for oxidative degradation of cellulose.
Sci Rep, 2016, 274 (1), 3338. DOI: 10.1038/srep28276 HAL: hal-01413367v1

Pierre Ceccaldi, Emilien Etienne, Sébastien Dementin, Bruno Guigliarelli, Christophe Léger, Bénédicte Burlat.
Mechanism of inhibition of NiFe hydrogenase by nitric oxide.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (4), 454 - 461. DOI: 10.1016/j.bbabio.2016.01.014 HAL: hal-01413192v1

Nolwenn Le Breton, Tiona Adrianaivomananjaona, Guillaume Gerbaud, Emilien Etienne, Elena Bisetto, Alain Dautant, Bruno Guigliarelli, Francis Haraux, Marlène Martinho, Valérie Belle.
Dimerization interface and dynamic properties of yeast IF1 revealed by Site-Directed Spin Labeling EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (1), 89 - 97. DOI: 10.1016/j.bbabio.2015.10.010 HAL: hal-01413333v1

Stéphane Grimaldi, Frédéric Biaso, Bénédicte Burlat, Bruno Guigliarelli.
Electron paramagnetic resonance studies of molybdenum enzymes.
Molybdenum and Tungsten Enzymes : Spectroscopic and Theoretical Investigations. RSC Metallobiology Series. Editors: Russ Hille, Carola Schulzke, Martin L Kirk, 2016, 68 - 120. DOI: 10.1039/9781782628842-00068 HAL: hal-01441142v1

2015

Pierre Ceccaldi, Julia Rendon, Christophe Léger, René Toci, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi, Vincent Fourmond.
Reductive activation of E. coli respiratory nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (10), 1055 - 1063. DOI: 10.1016/j.bbabio.2015.06.007 HAL: hal-01429839v1

Julia Rendon, Eric Pilet, Zeinab Fahs, Farida Seduk, Léa Sylvi, Mahmoud Hajj Chehade, Fabien Pierrel, Bruno Guigliarelli, Axel Magalon, Stephane Grimaldi.
Demethylmenaquinol is a substrate of Escherichia coli nitrate reductase A (NarGHI) and forms a stable semiquinone intermediate at the NarGHI quinol oxidation site.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (8), 739 - 747. DOI: 10.1016/j.bbabio.2015.05.001 HAL: hal-01429032v1

Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 - 7. DOI: 10.3389/fmolb.2015.00021 HAL: hal-01429982v1

Abbas Abou-Hamdan, Pierre Ceccaldi, Hugo Lebrette, Oscar Gutiérrez-Sanz, Pierre Richaud, Laurent Cournac, Bruno Guigliarelli, Antonio L. De Lacey, Christophe Léger, Anne Volbeda, Bénédicte Burlat, Sébastien Dementin.
A Threonine Stabilizes the NiC and NiR Catalytic Intermediates of [NiFe]-hydrogenase.
J. Biol. Chem., 2015, 290 (13), 8550 - 8558. DOI: 10.1074/jbc.M114.630491 HAL: hal-01149507v1

2014

Julien G.J. Jacques, Bénédicte Burlat, Pascal Arnoux, Monique Sabaty, Bruno Guigliarelli, Christophe Léger, David Pignol, Vincent Fourmond.
Kinetics of substrate inhibition of periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (10), 1801 - 1809. DOI: 10.1016/j.bbabio.2014.05.357 HAL: hal-01494456v1

Julien G.J. Jacques, Vincent Fourmond, Pascal Arnoux, Monique Sabaty, Emilien Etienne, Sandrine Grosse, Frédéric Biaso, Patrick Bertrand, David Pignol, Christophe Léger, Bruno Guigliarelli, Bénédicte Burlat.
Reductive activation in periplasmic nitrate reductase involves chemical modifications of the Mo-cofactor beyond the first coordination sphere of the metal ion.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (2), 277 - 286. DOI: 10.1016/j.bbabio.2013.10.013 HAL: hal-01481487v1

Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Diversification of EPR signatures in site directed spin labeling using a β-phosphorylated nitroxide.
Phys. Chem. Chem. Phys., 2014, 16, 4202 - 4209. DOI: 10.1039/c3cp54816c HAL: hal-01460516v1

2013

Stéphane Grimaldi, Barbara Schoepp-Cothenet, Pierre Ceccaldi, Bruno Guigliarelli, Axel Magalon.
The prokaryotic Mo/W-bisPGD enzymes family: A catalytic workhorse in bioenergetic.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (8-9), 1048 - 1085. DOI: 10.1016/j.bbabio.2013.01.011 HAL: hal-03549630

Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 - 1117. DOI: 10.1021/bc4000542 HAL: hal-01460460v1

Elisabetta Mileo, Magali Lorenzi, Jenny Erales, Sabrina Lignon, Carine Puppo, Nolwenn Le Breton, Emilien Etienne, Sylvain R. A. Marque, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
Dynamics of the intrinsically disordered protein CP12 in its association with GAPDH in the green alga Chlamydomonas reinhardtii: a fuzzy complex.
Mol. BioSyst., 2013, 1 (11), 349. DOI: 10.1039/c3mb70190e HAL: hal-01460463v1

Abbas Abou Hamdan, Bénédicte Burlat, Oscar Gutiérrez-Sanz, Pierre-Pol Liebgott, Carole Baffert, Antonio L De Lacey, Marc Rousset, Bruno Guigliarelli, Christophe Léger, Sébastien Dementin.
O2-independent formation of the inactive states of NiFe hydrogenase.
Nat Chem Biol, 2013, 9, 15 - 17. DOI: 10.1038/NCHEMBIO.1110 HAL: hal-01556905v1

2012

Rodrigo Arias-Cartin, Stéphane Grimaldi, Pascal Arnoux, Bruno Guigliarelli, Axel Magalon.
Cardiolipin binding in bacterial respiratory complexes: Structural and functional implications.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012, 1817 (10), 1937 - 1949. DOI: 10.1016/j.bbabio.2012.04.005 HAL: hal-03549629v1

Marlène Martinho, Johnny Habchi, Zeina El Habre, Léo Nesme, Bruno Guigliarelli, Valérie Belle, Sonia Longhi.
Assessing induced folding within the intrinsically disordered C-terminal domain of the Henipavirus nucleoproteins by site-directed spin labeling EPR spectroscopy.
J. Biomol. Struct. Dyn., 2012, 31, 453-471. DOI: 10.1080/07391102.2012.706068

Frédéric Biaso, Bénédicte Burlat, Bruno Guigliarelli.
DFT Investigation of the Molybdenum Cofactor in Periplasmic Nitrate Reductases: Structure of the Mo(V) EPR-Active Species.
Inorg. Chem., 2012, 51 (6), 3409 - 3419. DOI: 10.1021/ic201533p

Johnny Habchi, Marlène Martinho, Antoine Gruet, Bruno Guigliarelli, Sonia Longhi, Valérie Belle.
Monitoring structural transitions in IDPs by site-directed spin-labeling EPR spectroscopy.
Intrinsically Disordered Protein Analysis Volume 1, Methods and Experimental Tools, 2012, 361-386. DOI: 10.1007/978-1-61779-927-3_21

2011

Stéphane Grimaldi, Rodrigo Arias-Cartin, Pascal Lanciano, Sevdalina Lyubenova, Rodolphe Szenes, Burkhard Endeward, Thomas F. Prisner, Bruno Guigliarelli, Axel Magalon.
Determination of the Proton Environment of High Stability Menasemiquinone Intermediate in Escherichia coli Nitrate Reductase A by Pulsed EPR.
J. Biol. Chem., 2011, 287 (7), 4662 - 4670. DOI: 10.1074/jbc.M111.325100 HAL: hal-03248799v1

Frédéric Halgand, Johnny Habchi, Laetitia Cravello, Marlène Martinho, Bruno Guigliarelli, Sonia Longhi.
Dividing To Unveil Protein Microheterogeneities: Traveling Wave Ion Mobility Study.
Anal. Chem., 2011, 83 (19), 7306 - 7315. DOI: 10.1021/ac200994c HAL: hal-00677256v1

Magali Lorenzi, Carine Puppo, Régine Lebrun, Sabrina Lignon, Valérie Roubaud, Marlène Martinho, Elisabetta Mileo, Paul Tordo, Sylvain R. A. Marque, Brigitte Gontero, Bruno Guigliarelli, Valérie Belle.
Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins.
Angew. Chem. Int. Ed., 2011, 50, 9108 - 9111. DOI: 10.1002/anie.201102539 HAL: hal-00677247v1

Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 - 10221. DOI: 10.1021/ja202615a HAL: hal-00677402v1

R. Arias-Cartin, S. Grimaldi, J. Pommier, P. Lanciano, C. Schaefer, P. Arnoux, G. Giordano, B. Guigliarelli, A. Magalon.
Cardiolipin-based respiratory complex activation in bacteria.
Proceedings of the National Academy of Sciences, 2011, 108 (19), 7781 - 7786. DOI: 10.1073/pnas.1010427108 HAL: hal-00677353v1

Sonia Longhi, Valérie Belle, André Fournel, Bruno Guigliarelli, Frédéric Carrière.
Probing structural transitions in both structured and disordered proteins using site-directed spin-labeling EPR spectroscopy.
J. Peptide Sci., 2011, 17 (5), 315 - 328. DOI: 10.1002/psc.1344

Pierre-Pol Liebgott, Antonio L. de Lacey, Bénédicte Burlat, Laurent Cournac, Pierre Richaud, Myriam Brugna, Victor M. Fernandez, Bruno Guigliarelli, Marc Rousset, Christophe Léger, Sébastien Dementin.
Original Design of an Oxygen-Tolerant [NiFe] Hydrogenase: Major Effect of a Valine-to-Cysteine Mutation near the Active Site.
J. Am. Chem. Soc., 2011, 133 (4), 986 - 997. DOI: 10.1021/ja108787s HAL: hal-01977599v1

2010

Fanny Leroux, Pierre-Pol Liebgott, Laurent Cournac, Pierre Richaud, Arlette Kpebe, Bénedicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Christophe Léger, Marc Rousset.
Is engineering O2-tolerant hydrogenases just a matter of reproducing the active sites of the naturally occurring O2-resistant enzymes?.
International Journal of Hydrogen Energy, 2010, 35 (19), 10770 - 10777. DOI: 10.1016/j.ijhydene.2010.02.071 HAL: hal-01977635v1

Rodrigo Arias-Cartin, Sevdalina Lyubenova, Pierre Ceccaldi, Thomas Prisner, Axel Magalon, Bruno Guigliarelli, Stéphane Grimaldi.
HYSCORE Evidence That Endogenous Mena- and Ubisemiquinone Bind at the Same Q Site (QD) of Escherichia coli Nitrate Reductase A.
J. Am. Chem. Soc., 2010, 132 (17), 5942 - 5943. DOI: 10.1021/ja1009234 HAL: hal-00677673v1

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Émilien Étienne, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants.
Biochemistry, 2010, 49 (11), 2424 - 2432. DOI: 10.1021/bi902140e HAL: hal-00677461

Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 6, 63 - 70. DOI: 10.1038/nchembio.276 HAL: hal-01977629

2009

Stéphane Grimaldi, Rodrigo Arias-Cartin, Pascal Lanciano, Sevdalina Lyubenova, Burkhard Endeward, Thomas F. Prisner, Axel Magalon, Bruno Guigliarelli.
Direct Evidence for Nitrogen Ligation to the High Stability Semiquinone Intermediate in Escherichia coli Nitrate Reductase A.
J. Biol. Chem., 2009, 285 (1), 179 - 187. DOI: 10.1074/jbc.M109.060251 HAL: hal-00677704v1

Sébastien Dementin, Fanny Leroux, Laurent Cournac, Antonio L. de Lacey, Anne Volbeda, Christophe Léger, Bénédicte Burlat, Nicolas Martinez, Stéphanie Champ, Lydie Martin, Oliver Sanganas, Michael Haumann, Víctor M. Fernández, Bruno Guigliarelli, Ju.
Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant.
J. Am. Chem. Soc., 2009, 131 (29), 10156 - 10164. DOI: 10.1021/ja9018258

Jenny Erales, Magali Lorenzi, Régine Lebrun, André Fournel, Emilien Etienne, Carine Courcelle, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
A New Function of GAPDH from Chlamydomonas reinhardtii: A Thiol−Disulfide Exchange Reaction with CP12.
Biochemistry, 2009, 48 (25), 6034 - 6040. DOI: 10.1021/bi900569h HAL: hal-00429826

2008

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Pascal Arnoux, Monique Sabaty, Séverine Boiry, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Major Mo(V) EPR Signature of Rhodobacter sphaeroides Periplasmic Nitrate Reductase Arising from a Dead-End Species That Activates upon Reduction. Relation to Other Molybdoenzymes from the DMSO Reductase Family.
J. Phys. Chem. B, 2008, 112 (48), 15478 - 15486. DOI: 10.1021/jp807092y HAL: hal-00336002

F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset, C. Leger.
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences, 2008, 105 (32), 11188 - 11193. DOI: 10.1073/pnas.0803689105 HAL: hal-00336010v1

Valérie Belle, Sabrina Rouger, Stéphanie Costanzo, Elodie Liquière, Janez Strancar, Bruno Guigliarelli, André Fournel, Sonia Longhi.
Mapping α-helical induced folding within the intrinsically disordered C-terminal domain of the measles virus nucleoprotein by site-directed spin-labeling EPR spectroscopy.
Proteins, 2008, 73 (4), 973 - 988. DOI: 10.1002/prot.22125

Carole Baffert, Marie Demuez, Laurent Cournac, Bénédicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Laurence Girbal, Christophe Léger.
Hydrogen-Activating Enzymes: Activity Does Not Correlate with Oxygen Sensitivity.
Angew. Chem. Int. Ed., 2008, 47 (11), 2052 - 2054. DOI: 10.1002/anie.200704313 HAL: hal-00336018

2007

Pascal Lanciano, Adrien Savoyant, Stéphane Grimaldi, Axel Magalon, Bruno Guigliarelli, Patrick Bertrand.
New Method for the Spin Quantitation of [4Fe−4S]+ Clusters with S = 3 / 2. Application to the FS0 Center of the NarGHI Nitrate Reductase from Escherichia coli.
J. Phys. Chem. B, 2007, 111 (48), 13632 - 13637. DOI: 10.1021/jp075243t HAL: hal-00336023v1

Sébastien Dementin, Pascal Arnoux, Bettina Frangioni, Sandrine Grosse, Christophe Léger, Bénédicte Burlat, Bruno Guigliarelli, Monique Sabaty, David Pignol.
Access to the Active Site of Periplasmic Nitrate Reductase: Insights from Site-Directed Mutagenesis and Zinc Inhibition Studies.
Biochemistry, 2007, 46 (34), 9713 - 9721. DOI: 10.1021/bi700928m HAL: hal-00336040

Patrick Bertrand, Bettina Frangioni, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Bruno Guigliarelli, David Pignol, Christophe Léger.
Effects of Slow Substrate Binding and Release in Redox Enzymes: Theory and Application to Periplasmic Nitrate Reductase.
J. Phys. Chem. B, 2007, 111 (34), 10300 - 10311. DOI: 10.1021/jp074340j HAL: hal-00336030

Pascal Lanciano, Axel Magalon, Patrick Bertrand, Bruno Guigliarelli, Stéphane Grimaldi.
High-Stability Semiquinone Intermediate in Nitrate Reductase A (NarGHI) from Escherichia coli Is Located in a Quinol Oxidation Site Close to Heme bD.
Biochemistry, 2007, 46 (18), 5323 - 5329. DOI: 10.1021/bi700074y HAL: hal-00336035v1

Pascal Lanciano, Alexandra Vergnes, Stéphane Grimaldi, Bruno Guigliarelli, Axel Magalon.
Biogenesis of a Respiratory Complex Is Orchestrated by a Single Accessory Protein.
J. Biol. Chem., 2007, 282 (24), 17468 - 17474. DOI: 10.1074/jbc.M700994200 HAL: hal-03549606v1

2006

M. Gabriela Almeida, Célia M. Silveira, Bruno Guigliarelli, Patrick Bertrand, José J.G. Moura, Isabel Moura, Christophe Léger.
A needle in a haystack: The active site of the membrane-bound complex cytochrome c nitrite reductase.
2006, 581 (2), 284 - 288. DOI: 10.1016/j.febslet.2006.12.023 HAL: hal-00336044

Gérard Lipowski, Ursula Liebl, Bruno Guigliarelli, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Conformation of the c 552 : aa 3 electron transfer complex in Paracoccus denitrificans studied by EPR on oriented samples.
2006, 580 (25), 5988 - 5992. DOI: 10.1016/j.febslet.2006.09.038 HAL: hal-00824462v1

M GUIRAL, P TRON, V BELLE, C AUBERT, C LEGER, B GUIGLIARELLI, M GIUDICIORTICONI.
Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties.
International Journal of Hydrogen Energy, 2006, 31 (11), 1424 - 1431. DOI: 10.1016/j.ijhydene.2006.06.007 HAL: hal-00335435

Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 - 5218. DOI: 10.1021/ja060233b HAL: hal-00335157v1

Christophe Léger, Florence Lederer, Bruno Guigliarelli, Patrick Bertrand.
Electron Flow in Multicenter Enzymes: Theory, Applications, and Consequences on the Natural Design of Redox Chains.
J. Am. Chem. Soc., 2006, 128 (1), 180 - 187. DOI: 10.1021/ja055275z HAL: hal-00336055v1

2005

Claude More, Marcel Asso, Guy Roger, Bruno Guigliarelli, Jorge Caldeira, José Moura, Patrick Bertrand.
Study of the Spin−Spin Interactions between the Metal Centers of Desulfovibrio gigas Aldehyde Oxidoreductase: Identification of the Reducible Sites of the [2Fe-2S] 1+,2+ Clusters.
Biochemistry, 2005, 44 (34), 11628 - 11635. DOI: 10.1021/bi0510025 HAL: hal-00336065v1

Marianne Guiral, Gisèle Leroy, Pierre Bianco, Philippe Gallice, Bruno Guigliarelli, Mireille Bruschi, Wolfgang Nitschke, Marie-Thérèse Giudici-Orticoni.
Interaction and electron transfer between the high molecular weight cytochrome and cytochrome c3 from Desulfovibrio vulgaris Hildenborough: Kinetic, microcalorimetric, EPR and electrochemical studies.
Biochimica et Biophysica Acta (BBA) - General Subjects, 2005, 1723 (1-3), 45 - 54. DOI: 10.1016/j.bbagen.2005.01.015 HAL: hal-00339978v1

Stéphane Grimaldi, Pascal Lanciano, Patrick Bertrand, Francis Blasco, Bruno Guigliarelli.
Evidence for an EPR-Detectable Semiquinone Intermediate Stabilized in the Membrane-Bound Subunit NarI of Nitrate Reductase A (NarGHI) from Escherichia coli.
Biochemistry, 2005, 44 (4), 1300 - 1308. DOI: 10.1021/bi048009r HAL: hal-03549620v1

2004

Christophe Léger, Sébastien Dementin, Patrick Bertrand, Marc Rousset, Bruno Guigliarelli.
Inhibition and Aerobic Inactivation Kinetics of Desulfovibrio fructosovorans NiFe Hydrogenase Studied by Protein Film Voltammetry.
J. Am. Chem. Soc., 2004, 126 (38), 12162 - 12172. DOI: 10.1021/ja046548d HAL: hal-00336069

Bettina Frangioni, Pascal Arnoux, Monique Sabaty, David Pignol, Patrick Bertrand, Bruno Guigliarelli, Christophe Léger.
In Rhodobacter sphaeroides Respiratory Nitrate Reductase, the Kinetics of Substrate Binding Favors Intramolecular Electron Transfer.
J. Am. Chem. Soc., 2004, 126 (5), 1328 - 1329. DOI: 10.1021/ja0384072 HAL: hal-00338609v1

2003

Marianne Brugna-Guiral, Pascale Tron, Wolfgang Nitschke, Karl-Otto Stetter, Benedicte Burlat, Bruno Guigliarelli, Mireille Bruschi, Marie Thérèse Giudici-Orticoni.
[NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics.
Extremophiles, 2003, 139 (2), 139 - 157. DOI: 10.1007/s00792-002-0306-3 HAL: hal-00343693v1

1999

Myriam Brugna, Wolfgang Nitschke, Marcel Asso, Bruno Guigliarelli, Danielle Lemesle-Meunier, Christian Schmidt.
Redox Components of Cytochrome bc -type Enzymes in Acidophilic Prokaryotes.
J. Biol. Chem., 1999, 274 (24), 16766 - 16772. DOI: 10.1074/jbc.274.24.16766

1997

Mireille Bruschi, Mireille Woudstra, Bruno Guigliarelli, Marcel Asso, Elisabeth Lojou, Yves Petillot, Chantal Abergel.
Biochemical and Spectroscopic Characterization of Two New Cytochromes Isolated from Desulfuromonas acetoxidans.
Biochemistry, 1997, 36 (35), 10601 - 10608. DOI: 10.1021/bi9707741