Christophe LEGER

Directeur de Recherche, CNRS

+33 4 91 16 45 29

Chef d'équipe / Group leader: BIP 06

Publications
ORCID id: 0000-0002-8871-6059

2024

Andrea Fasano, Aurore Jacq-Bailly, Jeremy Wozniak, Vincent Fourmond, Christophe Léger.
Catalytic Bias and Redox-Driven Inactivation of the Group B FeFe Hydrogenase CpIII.
ACS Catal., 2024, 14 (9), 7001 - 7010. DOI: 10.1021/acscatal.4c01352 HAL: hal-04570861v1 (dataset available here)

Andrea Fasano, Vincent Fourmond, Christophe Léger.
Outer-sphere effects on the O2 sensitivity, catalytic bias and catalytic reversibility of hydrogenases.
Chem. Sci., 2024, 15, 5418 - 5433. DOI: 10.1039/D4SC00691G HAL: hal-04550524v1 (dataset available here)

Andrea Fasano, Vincent Fourmond, Christophe Léger.
The difference bidirectionality makes to the kinetic modeling of molecular catalysis.
Curr. Op. Electrochem., 2024, 46, 101489. DOI: 10.1016/j.coelec.2024.101489 HAL: hal-04550686v1

Andrea Fasano, Carole Baffert, Conrad Schumann, Gustav Berggren, James A. Birrell, Vincent Fourmond, Christophe Léger.
Kinetic Modeling of the Reversible or Irreversible Electrochemical Responses of FeFe-Hydrogenases.
J. Am. Chem. Soc., 2024, 146 (2), 1455–1466. DOI: 10.1021/jacs.3c10693 HAL: hal-04372676v1

Ana Rita Oliveira, Cristiano Mota, Guilherme Vilela-Alves, Rita Rebelo Manuel, Neide Pedrosa, Vincent Fourmond, Kateryna Klymanska, Christophe Léger, Bruno Guigliarelli, Maria João Romão, Inês A. Cardoso Pereira.
An allosteric redox switch involved in oxygen protection in a CO2 reductase.
Nat Chem Biol, 2024, 20 (1), 111-119. DOI: 10.1038/s41589-023-01484-2 HAL: hal-04383526 (dataset available here)

Andrea Fasano, Vincent Fourmond, Christophe Léger.
Kinetic modeling of 2e-/1H+ and 2e-/2H+ bidirectional catalytic cycles.
Bioelectrochemistry, 2024, 108511 -. DOI: 10.1016/j.bioelechem.2023.108511 HAL: hal-04284492

2023

Andrea Fasano, Chloé Guendon, Aurore Jacq-Bailly, Arlette Kpebe, Jérémy Wozniak, Carole Baffert, Melisa del Barrio, Vincent Fourmond, Myriam Brugna, Christophe Léger.
A Chimeric NiFe Hydrogenase Heterodimer to Assess the Role of the Electron Transfer Chain in Tuning the Enzyme’s Catalytic Bias and Oxygen Tolerance.
J. Am. Chem. Soc., 2023, 145 (36), 20021–20030. DOI: 10.1021/jacs.3c06895 HAL: hal-04284474

Andrea Fasano, Aurore Bailly, Jeremy Wozniak, Vincent Fourmond, Christophe Léger.
Catalytic bias and redox-driven inactivation of ancestral FeFe hydrogenases from group B2.
bioRxiv, 2023. DOI: 10.1101/2023.06.23.541094 HAL: hal-04290784

Raphaël J. Labidi, Bruno Faivre, Philippe Carpentier, Giulia Veronesi, Albert Solé-Daura, Ragnar Bjornsson, Christophe Léger, Philipp Gotico, Yun Li, Mohamed Atta, Marc Fontecave.
Light-Driven Hydrogen Evolution Reaction Catalyzed by a Molybdenum–Copper Artificial Hydrogenase.
J. Am. Chem. Soc., 2023. DOI: 10.1021/jacs.3c01350 HAL: hal-04128647

Anna Aldinio-Colbachini, Andrea Fasano, Chloé Guendon, Aurore Bailly, Jeremy Wozniak, Carole Baffert, Arlette Kpebe, Christophe Léger, Myriam Brugna, Vincent Fourmond.
Transport limited adsorption experiments give a new lower estimate of the turnover frequency of Escherichia coli hydrogenase 1.
BBA Advances, 2023, 100090 -. DOI: 10.1016/j.bbadva.2023.100090 HAL: hal-04221197

Cheriehan Hessin, Jules Schleinitz, Nolwenn Le Breton, Sylvie Choua, Laurence Grimaud, Vincent Fourmond, Marine Desage-El Murr, Christophe Léger.
Assessing the Extent of Potential Inversion by Cyclic Voltammetry: Theory, Pitfalls, and Application to a Nickel Complex with Redox-Active Iminosemiquinone Ligands.
Inorg. Chem., 2023. DOI: 10.1021/acs.inorgchem.2c04365 HAL: hal-04071135

Marta Meneghello, Alexandre Uzel, Marianne Broc, Rita R Manuel, Axel Magalon, Inês A. C. Pereira, Christophe Léger, Anne Walburger, Vincent Fourmond.
Electrochemical Kinetics Support a Second Coordination Sphere Mechanism in Metal‐Based Formate Dehydrogenase.
Angew Chem Int Ed, 2023. DOI: 10.1002/anie.202212224 HAL: hal-03965660

2022

Andreas Rutz, Chandan K. Das, Andrea Fasano, Jan Jaenecke, Shanika Yadav, Ulf-Peter Apfel, Vera Engelbrecht, Vincent Fourmond, Christophe Léger, Lars V. Schäfer, Thomas Happe.
Increasing the O2 Resistance of the [FeFe]-Hydrogenase CbA5H through Enhanced Protein Flexibility.
ACS Catal., 2022, 856 - 865. DOI: 10.1021/acscatal.2c04031 HAL: hal-03932821

Sven T. Stripp, Benjamin R. Duffus, Vincent Fourmond, Christophe Léger, Silke Leimkühler, Shun Hirota, Yilin Hu, Andrew Jasniewski, Hideaki Ogata, Markus W. Ribbe.
Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.
Chem. Rev., 2022, 122 (14), 11900 - 11973. DOI: 10.1021/acs.chemrev.1c00914 HAL: hal-03741456

2021

Christina Felbek, Federica Arrigoni, David de Sancho, Aurore Jacq-Bailly, Robert B. Best, Vincent Fourmond, Luca Bertini, Christophe Léger.
Mechanism of Hydrogen Sulfide-Dependent Inhibition of FeFe Hydrogenase.
ACS Catal., 2021, 15162 - 15176. DOI: 10.1021/acscatal.1c04838 HAL: hal-03468382

Andrea Fasano, Henrik Land, Vincent Fourmond, Gustav Berggren, Christophe Léger.
Reversible or Irreversible Catalysis of H+/H2 Conversion by FeFe Hydrogenases.
J. Am. Chem. Soc., 2021. DOI: 10.1021/jacs.1c09554 HAL: hal-03445761

Marta Meneghello, Christophe Léger, Vincent Fourmond.
Electrochemical studies of CO2‐reducing metalloenzymes.
Chem. Eur. J., 2021. DOI: 10.1002/chem.202102702 HAL: hal-03358088

Matteo Sensi, Carole Baffert, Vincent Fourmond, Luca De Gioia, Luca Bertini, Christophe Léger.
Photochemistry and photoinhibition of the H-cluster of FeFe-hydrogenases.
Sustainable Energy Fuels, 2021, 5, 4248-4260. DOI: 10.1039/D1SE00756D HAL: hal-03358063

Vincent Fourmond, Christophe Léger.
Theoretical Understanding of the Penetration of O2 in Enzymatic Redox Polymer Films: The Case of Unidirectional Catalysis and Irreversible Inactivation in a Film of Arbitrary Thickness.
ChemElectroChem, 2021, 8 (13), 2607 - 2615. DOI: 10.1002/celc.202100586 HAL: hal-03277147v1

Christophe Léger.
Cinétique enzymatique.
Encyclopédie Universalis, 2021. (voir / see) HAL: hal-03367664

Vincent Fourmond, Nicolas Plumeré, Christophe Léger.
Reversible catalysis.
Nat Rev Chem, 2021, 5 (5). DOI: 10.1038/s41570-021-00268-3 HAL: hal-03215154

Steffen Hardt, Stefanie Stapf, Dawit T. Filmon, James A. Birrell, Olaf Rüdiger, Vincent Fourmond, Christophe Léger, Nicolas Plumeré.
Reversible H2 oxidation and evolution by hydrogenase embedded in a redox polymer film.
Nature Catalysis, 2021, 4, 251-258. DOI: 10.1038/s41929-021-00586-1 HAL: hal-03215114

Marta Meneghello, Ana Rita Oliveira, Aurore Jacq-Bailly, Inês Cardoso Peirera, Christophe Léger, Vincent Fourmond.
Formate dehydrogenases reduce CO2 rather than HCO3‐: an electrochemical demonstration.
Angew. Chem. Int. Ed., 2021. DOI: 10.1002/anie.202101167 HAL: hal-03181024

Martin Winkler, Jifu Duan, Andreas Rutz, Christina Felbek, Lisa Scholtysek, Oliver Lampret, Jan Jaenecke, Ulf-Peter Apfel, Gianfranco Gilardi, Francesca Valetti, Vincent Fourmond, Eckhard Hofmann, Christophe Léger, Thomas Happe.
A safety cap protects hydrogenase from oxygen attack.
Nat Commun, 2021, 12 (1). DOI: 10.1038/s41467-020-20861-2 HAL: hal-03129104

Aurore Jacq-Bailly, Martino Benvenuti, Natalie Payne, Arlette Kpebe, Christina Felbek, Vincent Fourmond, Christophe Léger, Myriam Brugna, Carole Baffert.
Electrochemical Characterization of a Complex FeFe Hydrogenase, the Electron-Bifurcating Hnd From Desulfovibrio fructosovorans.
Front. Chem., 2021, 8. DOI: 10.3389/fchem.2020.573305 HAL: hal-03103487v1

Christina Felbek, Steffen Hardt, Cecilia Papini, Debajyoti Pramanik, Vincent Artero, Marc Fontecave, Vincent Fourmond, Nicolas Plumere, Christophe Léger.
Artificial maturation of [FeFe] hydrogenase in a redox polymer film.
Chem. Commun., 2021. DOI: 10.1039/D0CC08168J HAL: hal-03102775v1

2020

Vincent Fourmond, Christophe Léger.
Numerical computations of Marcus–hush–Chidsey electron transfer rate constants.
Journal of Electroanalytical Chemistry, 2020, 114762 -. DOI: 10.1016/j.jelechem.2020.114762 HAL: hal-02968022

Elizabeth Charlotte Wittenborn, Chloé Guendon, Mériem Merrouch, Martino Benvenuti, Vincent Fourmond, Christophe Leger, Catherine L. Drennan, and Sébastien Dementin.
The solvent-exposed Fe-S D-cluster contributes to oxygen-resistance in D. vulgaris Ni-Fe carbon monoxide dehydrogenase.
ACS Cat., 2020, 10, 7328–7335. DOI: 10.1021/acscatal.0c00934 HAL: hal-02936282

Martino Benvenuti, Marta Meneghello, Chloé Guendon, Aurore Jacq-Bailly, Jae-hun Jeoung, Holger Dobbek, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
The two CO-dehydrogenases of Thermococcus sp. AM4.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 148188 -. DOI: 10.1016/j.bbabio.2020.148188 HAL: hal-02537797

2019

Huaiguang Li, Darren Buesen, Sébastien Dementin, Christophe Léger, Vincent Fourmond, Nicolas Plumeré.
Complete Protection of O2-Sensitive Catalysts in Thin Films.
J. Am. Chem. Soc., 2019, 141 (42), 16734 - 16742. DOI: 10.1021/jacs.9b06790 HAL: hal-02369997v1

Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira.
A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase.
ACS Catal., 2019, 9 (9), 8509 - 8519. DOI: 10.1021/acscatal.9b02347 HAL: hal-02278341v1

Elizabeth C. Wittenborn, Steven E. Cohen, Mériem Merrouch, Christophe Léger, Vincent Fourmond, Sébastien Dementin, Catherine L. Drennan.
Structural insight into metallocofactor maturation in carbon monoxide dehydrogenase.
J. Biol. Chem., 2019, 257 (35), 6259 - 13026. DOI: 10.1074/jbc.RA119.009610 HAL: hal-02188452v1

Vincent Fourmond, Eric S. Wiedner, Wendy J. Shaw, Christophe Léger.
Understanding and Design of Bidirectional and Reversible Catalysts of Multielectron, Multistep Reactions.
J. Am. Chem. Soc., 2019, 141 (28), 11269 - 11285. DOI: 10.1021/jacs.9b04854 HAL: hal-02179792v1

Melisa del Barrio, Chloé Guendon, Arlette Kpebe, Carole Baffert, Vincent Fourmond, Myriam Brugna, Christophe Léger.
Valine-to-Cysteine Mutation Further Increases the Oxygen Tolerance of Escherichia coli NiFe Hydrogenase Hyd-1.
ACS Catal., 2019, 9 (5), 4084 - 4088. DOI: 10.1021/acscatal.9b00543 HAL: hal-02104593v1

2018

Elizabeth C Wittenborn, Mériem Merrouch, Chie Ueda, Laura Fradale, Christophe Léger, Vincent Fourmond, Maria-Eirini Pandelia, Sébastien Dementin, Catherine L Drennan.
Redox-dependent rearrangements of the NiFeS cluster of carbon monoxide dehydrogenase.
2018, 119, 13690 -. DOI: 10.7554/eLife.39451 HAL: hal-01917213v1

Giorgio Caserta, Cecilia Papini, Agnieszka Adamska-Venkatesh, Ludovic Pecqueur, Constanze Sommer, Edward Reijerse, Wolfgang Lubitz, Charles Gauquelin, Isabelle Meynial-Salles, Debajyoti Pramanik, Vincent Artero, Mohamed Atta, Melisa del Barrio, Bruno Faiv.
Engineering an [FeFe]-Hydrogenase: Do Accessory Clusters Influence O 2 Resistance and Catalytic Bias?.
J. Am. Chem. Soc., 2018, 140 (16), 5516 - 5526. DOI: 10.1021/jacs.8b01689 HAL: hal-01759288v1

Melisa del Barrio, Matteo Sensi, Laura Fradale, Maurizio Bruschi, Claudio Greco, Luca de Gioia, Luca Bertini, Vincent Fourmond, Christophe Léger.
Interaction of the H-Cluster of FeFe Hydrogenase with Halides.
J. Am. Chem. Soc., 2018, 140 (16), 5485 - 5492. DOI: 10.1021/jacs.8b01414 HAL: hal-01759718v1

Melisa del Barrio, Matteo Sensi, Christophe Orain, Carole Baffert, Sébastien Dementin, Vincent Fourmond, Christophe Léger.
Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels.
Acc. Chem. Res., 2018, 51 (3), 769 - 777. DOI: 10.1021/acs.accounts.7b00622 HAL: hal-01745738v1

Mériem Merrouch, Martino Benvenuti, Marco Lorenzi, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
Maturation of the [Ni–4Fe–4S] active site of carbon monoxide dehydrogenases.
J Biol Inorg Chem, 2018, 37 (4), 80 - 620. DOI: 10.1007/s00775-018-1541-0 HAL: hal-01816335v1

Matteo Sensi, Christophe Orain, Carole Baffert, Vincent Fourmond et Christophe Léger.
La réaction des hydrogénases FeFe avec le dioxygène: étude expérimentale et théorique.
L'Actualité Chimique, 2018 (426). (voir / see)

Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, David Guieysse, Laurence Girbal, Vincent Fourmond, Isabelle André, Bruno Guigliarelli, Christophe Léger, Philippe Soucaille, Isabelle Meynial-Salles.
Roles of the F-domain in [FeFe] hydrogenase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2018, 1859 (2), 69 - 77. DOI: 10.1016/j.bbabio.2017.08.010 HAL: cea-01950818

2017

Lilith Domnik, Meriem Merrouch, Sebastian Goetzl, Jae-Hun Jeoung, Christophe Léger, Sébastien Dementin, Vincent Fourmond, Holger Dobbek.
CODH-IV: A High-Efficiency CO-Scavenging CO Dehydrogenase with Resistance to O2.
Angew. Chem. Int. Ed., 2017, 121 (48), 11034 - 15469. DOI: 10.1002/anie.201709261 HAL: hal-01696167v1

Matteo Sensi, Melisa del Barrio, Carole Baffert, Vincent Fourmond, Christophe Léger.
New perspectives in hydrogenase direct electrochemistry.
Current Opinion in Electrochemistry, 2017, 5 (1), 135 - 145. DOI: 10.1016/j.coelec.2017.08.005 HAL: hal-01614142v1

Matteo Sensi, Carole Baffert, Laura Fradale, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Maurizio Bruschi, Vincent Fourmond, Christophe Léger, Luca Bertini.
Photoinhibition of FeFe Hydrogenase.
ACS Catal., 2017, 7 (10), 7378 - 7387. DOI: 10.1021/acscatal.7b02252 HAL: hal-01613876v1

Meriem Merrouch, Jessica Hadj-Saïd, Christophe Léger, Sébastien Dementin, Vincent Fourmond.
Reliable estimation of the kinetic parameters of redox enzymes by taking into account mass transport towards rotating electrodes in protein film voltammetry experiments.
Electrochimica Acta, 2017, 245, 1059 - 1064. DOI: 10.1016/j.electacta.2017.03.114 HAL: hal-01614234v1

Christophe Léger.
Oxydation et production de dihydrogène : leçons de la nature.
Colloque “Biomimétisme et chimie durable”, Direction de la recherche et de l’innovation Service de la recherche, 2017. (voir / see)

Vincent Fourmond, Christophe Léger.
Dinitrogen Reduction: Interfacing the Enzyme Nitrogenase with Electrodes.
Angew. Chem. Int. Ed., 2017, 137 (16), 5494 - 4390. DOI: 10.1002/anie.201701179 HAL: hal-01514839v1

Vincent Fourmond, Christophe Léger.
Modelling the voltammetry of adsorbed enzymes and molecular catalysts.
Current Opinion in Electrochemistry, 2017, 1 (1), 110 - 120. DOI: 10.1016/j.coelec.2016.11.002 HAL: hal-01440516v1

Adam Kubas, Christophe Orain, David De Sancho, Laure Saujet, Matteo Sensi, Charles Gauquelin, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Carole Baffert, Vincent Fourmond, Robert B. Best, Jochen Blumberger, Christophe Léger.
Mechanism of O2 diffusion and reduction in FeFe hydrogenases.
Nature Chem, 2017, 11 (1), 205 - 95. DOI: 10.1038/nchem.2592 HAL: hal-01406025v1

2016

Matteo Sensi, Carole Baffert, Claudio Greco, Giorgio Caserta, Charles Gauquelin, Laure Saujet, Marc Fontecave, Souvik Roy, Vincent Artero, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Vincent Fourmond, Christophe Léger, Luca B.
Reactivity of the Excited States of the H-Cluster of FeFe Hydrogenases.
J. Am. Chem. Soc., 2016, 138 (41), 13612 - 13618. DOI: 10.1021/jacs.6b06603 HAL: hal-01889471v1

Pierre Ceccaldi, Emilien Etienne, Sébastien Dementin, Bruno Guigliarelli, Christophe Léger, Bénédicte Burlat.
Mechanism of inhibition of NiFe hydrogenase by nitric oxide.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (4), 454 - 461. DOI: 10.1016/j.bbabio.2016.01.014 HAL: hal-01413192v1

V. Fourmond, C. Léger.
Advances in Biochemical Engineering/Biotechnology.
2016, 48, 1400 - 41. DOI: 10.1007/10_2015_5016 HAL: hal-01413237v1

2015

Jessica Hadj-Saïd, Maria-Eirini Pandelia, Christophe Léger, Vincent Fourmond, Sébastien Dementin.
The Carbon Monoxide Dehydrogenase from Desulfovibrio vulgaris.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (12), 1574 - 1583. DOI: 10.1016/j.bbabio.2015.08.002 HAL: hal-01430965v1

Meriem Merrouch, Jessica Hadj-Saïd, Lilith Domnik, Holger Dobbek, Christophe Léger, Sébastien Dementin, Vincent Fourmond.
O2 Inhibition of Ni-Containing CO Dehydrogenase Is Partly Reversible.
Chem. Eur. J., 2015, 181 (52), 437 - 18938. DOI: 10.1002/chem.201502835 HAL: hal-01432205v1

Pierre Ceccaldi, Julia Rendon, Christophe Léger, René Toci, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi, Vincent Fourmond.
Reductive activation of E. coli respiratory nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (10), 1055 - 1063. DOI: 10.1016/j.bbabio.2015.06.007 HAL: hal-01429839v1

Christophe Orain, Laure Saujet, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Carole Baffert, Vincent Fourmond, Hervé Bottin, Christophe Léger.
Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 2 Demonstrate That the Reaction Is Partly Reversible.
J. Am. Chem. Soc., 2015, 137 (39), 12580 - 12587. DOI: 10.1021/jacs.5b06934 HAL: hal-01211469v1

Vincent Fourmond, Stefanie Stapf, Huaiguang Li, Darren Buesen, James Birrell, Olaf Rüdiger, Wolfgang Lubitz, Wolfgang Schuhmann, Nicolas Plumeré, Christophe Léger.
Mechanism of Protection of Catalysts Supported in Redox Hydrogel Films.
J. Am. Chem. Soc., 2015, 137 (16), 5494 - 5505. DOI: 10.1021/jacs.5b01194 HAL: hal-01428049v1

Abbas Abou-Hamdan, Pierre Ceccaldi, Hugo Lebrette, Oscar Gutiérrez-Sanz, Pierre Richaud, Laurent Cournac, Bruno Guigliarelli, Antonio L. De Lacey, Christophe Léger, Anne Volbeda, Bénédicte Burlat, Sébastien Dementin.
A Threonine Stabilizes the NiC and NiR Catalytic Intermediates of [NiFe]-hydrogenase.
J. Biol. Chem., 2015, 290 (13), 8550 - 8558. DOI: 10.1074/jbc.M114.630491 HAL: hal-01149507v1

Vincent Fourmond, Carole Baffert, Sébastien Dementin, Christophe Léger.
L’électrochimie, un outil pour étudier les mécanismes enzymatiques.
L'Actualité Chimique, 2015 (392).

Pierre Ceccaldi, Marta C. Marques, Vincent Fourmond, Inês Cardoso Pereira, Christophe Léger.
Oxidative inactivation of NiFeSe hydrogenase.
Chem. Commun., 2015, 562 (75), 43 - 14226. DOI: 10.1039/C5CC05930E HAL: hal-01211493v1

2014

Julien G.J. Jacques, Bénédicte Burlat, Pascal Arnoux, Monique Sabaty, Bruno Guigliarelli, Christophe Léger, David Pignol, Vincent Fourmond.
Kinetics of substrate inhibition of periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (10), 1801 - 1809. DOI: 10.1016/j.bbabio.2014.05.357 HAL: hal-01494456v1

Vincent Fourmond, Claudio Greco, Kateryna Sybirna, Carole Baffert, Po-Hung Wang, Pierre Ezanno, Marco Montefiori, Maurizio Bruschi, Isabelle Meynial-Salles, Philippe Soucaille, Jochen Blumberger, Hervé Bottin, Luca De Gioia, Christophe Léger.
The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster.
Nature Chem, 2014, 130 (4), 4533 - 342. DOI: 10.1038/nchem.1892 HAL: hal-01481520v1

Julien G.J. Jacques, Vincent Fourmond, Pascal Arnoux, Monique Sabaty, Emilien Etienne, Sandrine Grosse, Frédéric Biaso, Patrick Bertrand, David Pignol, Christophe Léger, Bruno Guigliarelli, Bénédicte Burlat.
Reductive activation in periplasmic nitrate reductase involves chemical modifications of the Mo-cofactor beyond the first coordination sphere of the metal ion.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2014, 1837 (2), 277 - 286. DOI: 10.1016/j.bbabio.2013.10.013 HAL: hal-01481487v1

Claudio Greco, Vincent Fourmond, Carole Baffert, Po-hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger.
Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes.
Energy Environ. Sci., 2014, 108 (11), 2066 - 3573. DOI: 10.1039/C4EE01848F HAL: hal-01211585v1

Viviane Hajj, Carole Baffert, Kateryna Sybirna, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Vincent Fourmond, Christophe Léger.
FeFe hydrogenase reductive inactivation and implication for catalysis.
Energy Environ. Sci., 2014, 6 (2), 2460 - 719. DOI: 10.1039/C3EE42075B HAL: hal-01481475v1

2013

Toshiko Miyake, Maurizio Bruschi, Ugo Cosentino, Carole Baffert, Vincent Fourmond, Christophe Léger, Giorgio Moro, Luca De Gioia, Claudio Greco.
Does the environment around the H-cluster allow coordination of the pendant amine to the catalytic iron center in [FeFe] hydrogenases? Answers from theory.
J Biol Inorg Chem, 2013, 13 (6), 3580 - 700. DOI: 10.1007/s00775-013-1014-4 HAL: hal-03023214

Kateryna Sybirna, Pierre Ezanno, Carole Baffert, Christophe Léger, Hervé Bottin.
Arginine171 of Chlamydomonas reinhardtii [Fe–Fe] hydrogenase HydA1 plays a crucial role in electron transfer to its catalytic center.
International Journal of Hydrogen Energy, 2013, 38 (7), 2998 - 3002. DOI: 10.1016/j.ijhydene.2012.12.078

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Thomas Lautier, Abbas Abou Hamdan, Sébastien Dementin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Christophe Léger.
Steady-State Catalytic Wave-Shapes for 2-Electron Reversible Electrocatalysts and Enzymes.
J. Am. Chem. Soc., 2013, 135 (10), 3926 - 3938. DOI: 10.1021/ja311607s HAL: hal-01268145v1

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Sébastien Dementin, Abbas Abou-Hamdan, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
The mechanism of inhibition by H2 of H2-evolution by hydrogenases.
Chem. Commun., 2013, 109 (61), 19916 -. DOI: 10.1039/C3CC43297A HAL: hal-01268212v1

Abbas Abou Hamdan, Bénédicte Burlat, Oscar Gutiérrez-Sanz, Pierre-Pol Liebgott, Carole Baffert, Antonio L De Lacey, Marc Rousset, Bruno Guigliarelli, Christophe Léger, Sébastien Dementin.
O2-independent formation of the inactive states of NiFe hydrogenase.
Nat Chem Biol, 2013, 9, 15 - 17. DOI: 10.1038/NCHEMBIO.1110 HAL: hal-01556905v1

2012

A. Abou Hamdan, P.-P. Liebgott, V. Fourmond, O. Gutierrez-Sanz, A. L. De Lacey, P. Infossi, M. Rousset, S. Dementin, C. Leger.
Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants.
Proceedings of the National Academy of Sciences, 2012, 76 (1-2), 141 - 19921. DOI: 10.1073/pnas.1212258109 HAL: hal-01825482v1

Carole Baffert, Kateryna Sybirna, Pierre Ezanno, Thomas Lautier, Viviane Hajj, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
Covalent Attachment of FeFe Hydrogenases to Carbon Electrodes for Direct Electron Transfer.
Anal. Chem., 2012, 84 (18), 7999 - 8005. DOI: 10.1021/ac301812s HAL: hal-01268277v1

Abbas Abou Hamdan, Sébastien Dementin, Pierre-Pol Liebgott, Oscar Gutierrez-Sanz, Pierre Richaud, Antonio L. De Lacey, Marc Rousset, Patrick Bertrand, Laurent Cournac, Christophe Léger.
Understanding and Tuning the Catalytic Bias of Hydrogenase.
J. Am. Chem. Soc., 2012, 134 (20), 8368 - 8371. DOI: 10.1021/ja301802r HAL: hal-00748324v1

2011

Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 - 10221. DOI: 10.1021/ja202615a HAL: hal-00677402v1

Carole Baffert, Luca Bertini, Thomas Lautier, Claudio Greco, Kateryna Sybirna, Pierre Ezanno, Emilien Etienne, Philippe Soucaille, Patrick Bertrand, Hervé Bottin, Isabelle Meynial-Salles, Luca De Gioia, Christophe Léger.
CO Disrupts the Reduced H-Cluster of FeFe Hydrogenase. A Combined DFT and Protein Film Voltammetry Study.
J. Am. Chem. Soc., 2011, 133 (7), 2096 - 2099. DOI: 10.1021/ja110627b HAL: hal-02652182v1

Pierre-Pol Liebgott, Antonio L. de Lacey, Bénédicte Burlat, Laurent Cournac, Pierre Richaud, Myriam Brugna, Victor M. Fernandez, Bruno Guigliarelli, Marc Rousset, Christophe Léger, Sébastien Dementin.
Original Design of an Oxygen-Tolerant [NiFe] Hydrogenase: Major Effect of a Valine-to-Cysteine Mutation near the Active Site.
J. Am. Chem. Soc., 2011, 133 (4), 986 - 997. DOI: 10.1021/ja108787s HAL: hal-01977599v1

Olga Kolaj-Robin, Sarah R. O'Kane, Wolfgang Nitschke, Christophe Léger, Frauke Baymann, Tewfik Soulimane.
Biochemical and biophysical characterization of succinate: Quinone reductase from Thermus thermophilus.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2011, 1807 (1), 68 - 79. DOI: 10.1016/j.bbabio.2010.10.009 HAL: hal-00999887v1

Thomas Lautier, Pierre Ezanno, Carole Baffert, Vincent Fourmond, Laurent Cournac, Juan C. Fontecilla-Camps, Philippe Soucaille, Patrick Bertrand, Isabelle Meynial-Salles, Christophe Léger.
The quest for a functional substrate access tunnel in FeFe hydrogenase.
Faraday Discuss., 2011, 304, 55 - 407. DOI: 10.1039/c004099c HAL: hal-02646834

Pierre-Pol Liebgott, Sébastien Dementin, Christophe Léger, Marc Rousset.
Towards engineering O2-tolerance in [Ni–Fe] hydrogenases.
Energy Environ. Sci., 2011, 128 (1), 5209 - 41. DOI: 10.1039/c0ee00093k HAL: hal-01977604

2010

Fanny Leroux, Pierre-Pol Liebgott, Laurent Cournac, Pierre Richaud, Arlette Kpebe, Bénedicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Christophe Léger, Marc Rousset.
Is engineering O2-tolerant hydrogenases just a matter of reproducing the active sites of the naturally occurring O2-resistant enzymes?.
International Journal of Hydrogen Energy, 2010, 35 (19), 10770 - 10777. DOI: 10.1016/j.ijhydene.2010.02.071 HAL: hal-01977635v1

Maria-Eirini Pandelia, Vincent Fourmond, Pascale Tron-Infossi, Elisabeth Lojou, Patrick Bertrand, Christophe Léger, Marie-Thérèse Giudici-Orticoni, Wolfgang Lubitz.
Membrane-Bound Hydrogenase I from the Hyperthermophilic Bacterium Aquifex aeolicus: Enzyme Activation, Redox Intermediates and Oxygen Tolerance.
J. Am. Chem. Soc., 2010, 132 (20), 6991 - 7004. DOI: 10.1021/ja910838d HAL: hal-00686880v1

Vincent Fourmond, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Patrick Bertrand, Christophe Léger.
“Two-Step” Chronoamperometric Method for Studying the Anaerobic Inactivation of an Oxygen Tolerant NiFe Hydrogenase.
J. Am. Chem. Soc., 2010, 132 (13), 4848 - 4857. DOI: 10.1021/ja910685j HAL: hal-00677470

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Émilien Étienne, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants.
Biochemistry, 2010, 49 (11), 2424 - 2432. DOI: 10.1021/bi902140e HAL: hal-00677461

Vincent Fourmond, Monique Sabaty, Pascal Arnoux, Patrick Bertrand, David Pignol, Christophe Léger.
Reassessing the Strategies for Trapping Catalytic Intermediates during Nitrate Reductase Turnover.
J. Phys. Chem. B, 2010, 114 (9), 3341 - 3347. DOI: 10.1021/jp911443y HAL: hal-00677469

Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 6, 63 - 70. DOI: 10.1038/nchembio.276 HAL: hal-01977629

2009

K. H. Diêp Lê, Alain Boussac, Bettina Frangioni, Christophe Léger, Florence Lederer.
Interdomain Contacts in Flavocytochrome b 2, a Mutational Analysis.
Biochemistry, 2009, 48 (45), 10803 - 10809. DOI: 10.1021/bi901301r

Vincent Fourmond, Kevin Hoke, Hendrik A. Heering, Carole Baffert, Fanny Leroux, Patrick Bertrand, Christophe Léger.
SOAS: A free program to analyze electrochemical data and other one-dimensional signals.
Bioelectrochemistry, 2009, 76 (1-2), 141 - 147. DOI: 10.1016/j.bioelechem.2009.02.010 HAL: hal-00402940v1

Sébastien Dementin, Fanny Leroux, Laurent Cournac, Antonio L. de Lacey, Anne Volbeda, Christophe Léger, Bénédicte Burlat, Nicolas Martinez, Stéphanie Champ, Lydie Martin, Oliver Sanganas, Michael Haumann, Víctor M. Fernández, Bruno Guigliarelli, Ju.
Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant.
J. Am. Chem. Soc., 2009, 131 (29), 10156 - 10164. DOI: 10.1021/ja9018258

Vincent Fourmond, Thomas Lautier, Carole Baffert, Fanny Leroux, Pierre-Pol Liebgott, Sébastien Dementin, Marc Rousset, Pascal Arnoux, David Pignol, Isabelle Meynial-Salles, Phillippe Soucaille, Patrick Bertrand, Christophe Léger.
Correcting for Electrocatalyst Desorption and Inactivation in Chronoamperometry Experiments.
Anal. Chem., 2009, 81 (8), 2962 - 2968. DOI: 10.1021/ac8025702 HAL: hal-01825488v1

2008

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Pascal Arnoux, Monique Sabaty, Séverine Boiry, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Major Mo(V) EPR Signature of Rhodobacter sphaeroides Periplasmic Nitrate Reductase Arising from a Dead-End Species That Activates upon Reduction. Relation to Other Molybdoenzymes from the DMSO Reductase Family.
J. Phys. Chem. B, 2008, 112 (48), 15478 - 15486. DOI: 10.1021/jp807092y HAL: hal-00336002

F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset, C. Leger.
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences, 2008, 105 (32), 11188 - 11193. DOI: 10.1073/pnas.0803689105 HAL: hal-00336010v1

Christophe Léger, Patrick Bertrand.
Direct Electrochemistry of Redox Enzymes as a Tool for Mechanistic Studies.
Chem. Rev., 2008, 108 (7), 2379 - 2438. DOI: 10.1021/cr0680742 HAL: hal-01211439v1

Carole Baffert, Marie Demuez, Laurent Cournac, Bénédicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Laurence Girbal, Christophe Léger.
Hydrogen-Activating Enzymes: Activity Does Not Correlate with Oxygen Sensitivity.
Angew. Chem. Int. Ed., 2008, 47 (11), 2052 - 2054. DOI: 10.1002/anie.200704313 HAL: hal-00336018

2007

Sébastien Dementin, Pascal Arnoux, Bettina Frangioni, Sandrine Grosse, Christophe Léger, Bénédicte Burlat, Bruno Guigliarelli, Monique Sabaty, David Pignol.
Access to the Active Site of Periplasmic Nitrate Reductase: Insights from Site-Directed Mutagenesis and Zinc Inhibition Studies.
Biochemistry, 2007, 46 (34), 9713 - 9721. DOI: 10.1021/bi700928m HAL: hal-00336040

Patrick Bertrand, Bettina Frangioni, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Bruno Guigliarelli, David Pignol, Christophe Léger.
Effects of Slow Substrate Binding and Release in Redox Enzymes: Theory and Application to Periplasmic Nitrate Reductase.
J. Phys. Chem. B, 2007, 111 (34), 10300 - 10311. DOI: 10.1021/jp074340j HAL: hal-00336030

2006

M. Gabriela Almeida, Célia M. Silveira, Bruno Guigliarelli, Patrick Bertrand, José J.G. Moura, Isabel Moura, Christophe Léger.
A needle in a haystack: The active site of the membrane-bound complex cytochrome c nitrite reductase.
2006, 581 (2), 284 - 288. DOI: 10.1016/j.febslet.2006.12.023 HAL: hal-00336044

M GUIRAL, P TRON, V BELLE, C AUBERT, C LEGER, B GUIGLIARELLI, M GIUDICIORTICONI.
Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties.
International Journal of Hydrogen Energy, 2006, 31 (11), 1424 - 1431. DOI: 10.1016/j.ijhydene.2006.06.007 HAL: hal-00335435

Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 - 5218. DOI: 10.1021/ja060233b HAL: hal-00335157v1

Christophe Léger, Florence Lederer, Bruno Guigliarelli, Patrick Bertrand.
Electron Flow in Multicenter Enzymes: Theory, Applications, and Consequences on the Natural Design of Redox Chains.
J. Am. Chem. Soc., 2006, 128 (1), 180 - 187. DOI: 10.1021/ja055275z HAL: hal-00336055v1

2004

Christophe Léger, Sébastien Dementin, Patrick Bertrand, Marc Rousset, Bruno Guigliarelli.
Inhibition and Aerobic Inactivation Kinetics of Desulfovibrio fructosovorans NiFe Hydrogenase Studied by Protein Film Voltammetry.
J. Am. Chem. Soc., 2004, 126 (38), 12162 - 12172. DOI: 10.1021/ja046548d HAL: hal-00336069

Bettina Frangioni, Pascal Arnoux, Monique Sabaty, David Pignol, Patrick Bertrand, Bruno Guigliarelli, Christophe Léger.
In Rhodobacter sphaeroides Respiratory Nitrate Reductase, the Kinetics of Substrate Binding Favors Intramolecular Electron Transfer.
J. Am. Chem. Soc., 2004, 126 (5), 1328 - 1329. DOI: 10.1021/ja0384072 HAL: hal-00338609v1