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Directeur de Recherche, CNRS

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Frédéric Carrière, PhD, is Research Director at Centre National de la Recherche Scientifique (CNRS) and he currently works in the group of Enzymology of Supramolecular Systems at the Bioenergetics and Protein Engineering laboratory of CNRS and Aix Marseille University, Marseille, France. Over the last 30 years, his main research activities have been dealing with the biochemistry of lipids and lipolytic enzymes, with a strong interest in methods for studying lipid-protein interactions at interfaces and for lipolytic enzyme assays. Besides these molecular aspects based on protein engineering, biophysical methods and analytical chemistry, he investigated lipid digestion both in vitro and in vivo in the course of clinical studies. He has contributed to a better understanding of the relative contributions to lipid digestion of several enzymes, including gastric lipase, pancreatic lipase and pancreatic lipase-related protein 2. His studies have paid the way to the development of in vitro digestion methods, of lipase inhibitors for obesity treatment and of lipases for enzyme replacement therapy in patients with exocrine pancreatic insufficiency.
He is the author of >200 publications (h-index >46; see Researchgate ; ResearcherID: E-8408-2010; Google scholar ; ORCID ). Among other duties, Frédéric Carrière has been Unit Director of the laboratory of Enzymology at Interfaces and Physiology of Lipolysis (CNRS, Aix-Marseille University) from 2004 to 2017, Scientific Director of the LISA Carnot Institute (Lipids for Health and Industry), a consortium of laboratories aiming at partnership with industry in the field of lipid science and technology, from 2009 to 2015. He was president of GERLI (Groupe d’Etude et de Recherche en Lipidomique, the lipid section of the French Society for Biochemistry and Molecular Biology) from 2008 to 2014 and he is still a member of GERLI scientific board. Since 2007, he is a member of the Biologics Expert Committee at United States Pharmacopeia and Chair of the Pharmaceutical Enzyme Panel. Prior to join CNRS, he was employed as a researcher by the Jouveinal Laboratories (1988-1992; CIFRE contract)and Novo Nordisk A/S in Denmark(1992-1994). He was awarded the Chevreul medal in 2007 for his work on lipid science.

ORCID id: 0000-0003-4848-9418


Hélène Launay, Véronique Receveur-Bréchot, Frédéric Carrière, Brigitte Gontero.
Protein disorder in algal metabolism.
Atlas of Science, 2021. (voir / see) HAL: hal-03326941

Moulay Sahaka, Sawsan Amara, Jérôme Lecomte, Jean-David Rodier, Dominique Lafont, Pierre Villeneuve, Brigitte Gontero, Frédéric Carrière.
Quantitative monitoring of galactolipid hydrolysis by pancreatic lipase-related protein 2 using thin layer chromatography and thymol-sulfuric acid derivatization.
Journal of Chromatography B, 2021, 1173, 122674 -. DOI: 10.1016/j.jchromb.2021.122674 HAL: hal-03191084v1


Amal Salhi, Sawsan Amara, Pascal Mansuelle, Rémy Puppo, Régine Lebrun, Brigitte Gontero, Ahmed Aloulou, Frédéric Carrière.
Characterization of all the lipolytic activities in pancreatin and comparison with porcine and human pancreatic juices.
Biochimie, 2020, 169, 106 - 120. DOI: 10.1016/j.biochi.2019.07.004 HAL: hal-02265768v1

Laura Prioretti, Frédéric Carriere, Ben Field, Luisana Avilan, Marie-Hélène Montané, Benoît Menand, Brigitte Gontero.
Targeting TOR signaling for enhanced lipid productivity in algae.
Biochimie, 2020, 169, 12 - 17. DOI: 10.1016/j.biochi.2019.06.016 HAL: hal-02129344v1

Moulay Sahaka, Sawsan Amara, Jutarat Wattanakul, Mohamed A. Gedi, Noelia Aldai, Goetz Parsiegla, Jérôme Lecomte, John T. Christeller, David Gray, Brigitte Gontero, Pierre Villeneuve, Frédéric Carrière.
The digestion of galactolipids and its ubiquitous function in Nature for the uptake of the essential α-linolenic acid.
Food Funct., 2020. DOI: 10.1039/D0FO01040E HAL: hal-02903683v1

Frédéric Carrière, Sonia Longhi, Michel Record.
The endosomal lipid bis(monoacylglycero) phosphate as a potential key player in the mechanism of action of chloroquine against SARS-COV-2 and other enveloped viruses hijacking the endocytic pathway.
Biochimie, 2020. DOI: 10.1016/j.biochi.2020.05.013 HAL: hal-02693984v1


Jutarat Wattanakul, Moulay Sahaka, Sawsan Amara, Syamila Mansor, Brigitte Gontero, Frédéric Carrière, David Gray.
In vitro digestion of galactolipids from chloroplast-rich fraction (CRF) of postharvest, pea vine field residue (haulm) and spinach leaves.
Food Funct., 2019, 1760 (1), 1497 - 7817. DOI: 10.1039/c9fo01867k HAL: hal-02431885v1

Hélène Launay, Véronique Receveur-Bréchot, Frédéric Carrière, Brigitte Gontero.
Orchestration of algal metabolism by protein disorder.
Archives of Biochemistry and Biophysics, 2019, 672, 108070 -. DOI: 10.1016/ HAL: hal-02266032v1

Erik L. Jensen, Karen Yangüez, Frédéric Carrière, Brigitte Gontero.
Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration.
Biology, 2019, 25 (1), 1266 -. DOI: 10.3390/biology9010005 HAL: hal-02431926v1


Najeh Krayem, Goetz Parsiegla, Hélène Gaussier, Hanen Louati, Raida Jallouli, Pascal Mansuelle, Frédéric Carrière, Youssef Gargouri.
Functional characterization and FTIR-based 3D modeling of full length and truncated forms of Scorpio maurus venom phospholipase A 2.
Biochimica et Biophysica Acta (BBA) - General Subjects, 2018, 1862 (5), 1247 - 1261. DOI: 10.1016/j.bbagen.2018.02.004 HAL: hal-01739569v1

Inès Belhaj, Sawsan Amara, Goetz Parsiegla, Priscila Sutto-Ortiz, Moulay Sahaka, Hafedh Belghith, Audric Rousset, Dominique Lafont, Frédéric Carrière.
Galactolipase activity of Talaromyces thermophilus lipase on galactolipid micelles, monomolecular films and UV-absorbing surface-coated substrate.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2018, 1863 (9), 1006 - 1015. DOI: 10.1016/j.bbalip.2018.05.016 HAL: hal-01819569v1

Eduardo Mateos-Diaz, Jean-Claude Bakala N’Goma, Deborah Byrne, Sylvie Robert, Frédéric Carrière, Hélène Gaussier.
IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 1. Discriminative recognition of mixed micelles versus liposomes.
Chemistry and Physics of Lipids, 2018, 211, 52 - 65. DOI: 10.1016/j.chemphyslip.2017.02.005 HAL: hal-02325970v1

Eduardo Mateos-Diaz, Priscila Sutto-Ortiz, Moulay Sahaka, Deborah Byrne, Hélène Gaussier, Frédéric Carrière.
IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 2. Discriminative recognition of various micellar systems and characterization of PLRP2-DPPC-bile salt complexes.
Chemistry and Physics of Lipids, 2018, 211, 66 - 76. DOI: 10.1016/j.chemphyslip.2017.11.012 HAL: hal-02325977v1

Eduardo Mateos-Diaz, Priscila Sutto-Ortiz, Moulay Sahaka, Jorge A. Rodriguez, Frédéric Carrière.
IR spectroscopy analysis of pancreatic lipase-related protein 2 interaction with phospholipids: 3. Monitoring DPPC lipolysis in mixed micelles.
Chemistry and Physics of Lipids, 2018, 211, 77 - 85. DOI: 10.1016/j.chemphyslip.2017.11.009 HAL: hal-02325989v1

Lydie Humbert, Dominique Rainteau, Noshine Tuvignon, Claude Wolf, Philippe Seksik, René Laugier, Frédéric Carrière.
Postprandial bile acid levels in intestine and plasma reveal altered biliary circulation in chronic pancreatitis patients.
J. Lipid Res., 2018, 1801 (11), 665 - 2213. DOI: 10.1194/jlr.M084830 HAL: hal-01990491v1


Raida Jallouli, Goetz Parsiegla, Frédéric Carrière, Youssef Gargouri, Sofiane Bezzine.
Efficient heterologous expression of Fusarium solani lipase, FSL2, in Pichia pastoris, functional characterization of the recombinant enzyme and molecular modeling.
International Journal of Biological Macromolecules, 2017, 94, 61 - 71. DOI: 10.1016/j.ijbiomac.2016.09.030

Laura Prioretti, Luisana Avilan, Frédéric Carrière, Marie-Hélène Montané, Ben Field, Gérald Grégori, Benoît Menand, Brigitte Gontero.
The inhibition of TOR in the model diatom Phaeodactylum tricornutum promotes a get-fat growth regime.
Algal Research, 2017, 26, 265 - 274. DOI: 10.1016/j.algal.2017.08.009 HAL: hal-01696878v1


Jannet Kamoun, Mathieu Schué, Wala Messaoud, Justine Baignol, Vanessa Point, Eduardo Mateos-Diaz, Pascal Mansuelle, Youssef Gargouri, Goetz Parsiegla, Jean-François Cavalier, Frédéric Carrière, Ahmed Aloulou.
Biochemical characterization of Yarrowia lipolytica LIP8, a secreted lipase with a cleavable C-terminal region.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2015, 1851 (2), 129 - 140. DOI: 10.1016/j.bbalip.2014.10.012

Raida Jallouli, Houcemeddine Othman, Sawsan Amara, Goetz Parsiegla, Frédéric Carriere, Najet Srairi-abid, Youssef Gargouri, Sofiane Bezzine.
The galactolipase activity of Fusarium solani (phospho)lipase.
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2015, 1851 (3), 282 - 289. DOI: 10.1016/j.bbalip.2014.12.010


Malika Mekhalfi, Sawsan Amara, Sylvie Robert, Frédéric Carrière, Brigitte Gontero.
Effect of environmental conditions on various enzyme activities and triacylglycerol contents in cultures of the freshwater diatom, Asterionella formosa (Bacillariophyceae).
Biochimie, 2014, 101, 21 - 30. DOI: 10.1016/j.biochi.2013.12.004 HAL: hal-02165163v1


Vanessa Point, Anaïs Bénarouche, Ikram Jemel, Goetz Parsiegla, Gérard Lambeau, Frédéric Carrière, Jean-François Cavalier.
Effects of the propeptide of group X secreted phospholipase A2 on substrate specificity and interfacial activity on phospholipid monolayers.
Biochimie, 2013, 95 (1), 51 - 58. DOI: 10.1016/j.biochi.2012.07.023

Anaïs Bénarouche, Vanessa Point, Goetz Parsiegla, Frédéric Carrière, Jean-François Cavalier.
New insights into the pH-dependent interfacial adsorption of dog gastric lipase using the monolayer technique.
Colloids and Surfaces B: Biointerfaces, 2013, 111, 306 - 312. DOI: 10.1016/j.colsurfb.2013.06.025

Sawsan Amara, Dominique Lafont, Goetz Parsiegla, Vanessa Point, Aurélie Chabannes, Audric Rousset, Frédéric Carrière.
The galactolipase activity of some microbial lipases and pancreatic enzymes.
Eur. J. Lipid Sci. Technol., 2013, 1771 (4), 1446 - 451. DOI: 10.1002/ejlt.201300004


Vanessa Point, K.V.P. Pavan Kumar, Sylvain Marc, Vincent Delorme, Goetz Parsiegla, Sawsan Amara, Frédéric Carrière, Gérard Buono, Frédéric Fotiadu, Stéphane Canaan, Julien Leclaire, Jean-François Cavalier.
Analysis of the discriminative inhibition of mammalian digestive lipases by 3-phenyl substituted 1,3,4-oxadiazol-2(3H)-ones.
European Journal of Medicinal Chemistry, 2012, 58, 452 - 463. DOI: 10.1016/j.ejmech.2012.10.040 HAL: hal-00726237v1

Goetz Parsiegla, Bindesh Shrestha, Frédéric Carrière, Akos Vertes.
Direct Analysis of Phycobilisomal Antenna Proteins and Metabolites in Small Cyanobacterial Populations by Laser Ablation Electrospray Ionization Mass Spectrometry.
Anal. Chem., 2012, 84 (1), 34 - 38. DOI: 10.1021/ac202831w


Sonia Longhi, Valérie Belle, André Fournel, Bruno Guigliarelli, Frédéric Carrière.
Probing structural transitions in both structured and disordered proteins using site-directed spin-labeling EPR spectroscopy.
J. Peptide Sci., 2011, 106 (5), 21637 - 328. DOI: 10.1002/psc.1344