Chargée de Recherche, CNRS
+33 4 91 16 45 39
Equipe / Group: BIP 01
Mots clés: Copper stress – Chaperone proteins – Hsp33 – Zinc center – Escherichia coli
Microbiologiste et Biochimiste, spécialiste dans l’étude des protéines chaperons et des métalloprotéines, mon projet actuel consiste à décrypter l’impact d’un stress cuivre sur les bactéries et sur la protéostasie (homéostasie des protéines). Les travaux actuellement menés au laboratoire démontrent qu’un léger excès de cuivre dans une cellule entraine l’inactivation voire l’agrégation de nombreuses protéines. Dans ces conditions, les protéines chaperons jouent un rôle majeur. Nos travaux permettent une meilleure compréhension du mécanisme d’action du cuivre et ouvre la voie à l’optimisation de l’utilisation métal à des fins plus appliqués, le cuivre étant un puissant agent antimicrobien.
Microbiologist and biochemist, specialist in the study of molecular chaperones and metalloproteins, my current project consists in deciphering the impact of copper excess on bacteria and on the proteostasis (protein homeostasis). Current work in the laboratory shows that a slight excess of copper in a cell leads to the inactivation or even aggregation of many proteins. Under these conditions, chaperone proteins play a major role. Our work allows a better understanding of the mechanism of action of copper and opens the way to the optimization of this metal for biomedical purposes, copper being a powerful antimicrobial agent.
Publications
ORCID id: 0000-0002-9070-2334
2024
Magali Roger, Philippe Leone, Ninian J. Blackburn, Sam Horrell, Tadeo Moreno Chicano, Frédéric Biaso, Marie-Thérèse Giudici-Orticoni, Luciano A. Abriata, Greg L. Hura, Michael A. Hough, Giuliano Sciara, Marianne Ilbert.
Beyond the coupled distortion model: structural analysis of the single domain cupredoxin AcoP, a green mononuclear copper centre with original features.
Dalton Trans., 2024, - .
DOI: 10.1039/d3dt03372d
HAL: hal-04386843v1
2023
Solène G. Beauvois, Nicolas Flaugnatti, Marianne Ilbert, Marie Boyer, Esther Gavello-Fernandez, Rémi Fronzes, Dukas Jurėnas, Laure Journet.
The tip protein PAAR is required for the function of the type VI secretion system.
Microbiol Spectr, 2023, 11 (6), - .
DOI: 10.1128/spectrum.01478-23
Lana Weber, Atar Gilat, Nathanael Maillot, Deborah Byrne, Pascal Arnoux, Marie‐Thérèse Giudici‐Orticoni, Vincent Méjean, Marianne Ilbert, Olivier Genest, Rina Rosenzweig, Sébastien Dementin.
Bacterial adaptation to cold: Conservation of a short J‐domain co‐chaperone and its protein partners in environmental proteobacteria.
Environmental Microbiology, 2023.
DOI: 10.1111/1462-2920.16478
Enrico Falcone, Francesco Stellato, Bertrand Vileno, Merwan Bouraguba, Vincent Lebrun, Marianne Ilbert, Silvia Morante, Peter Faller.
Revisiting the pro-oxidant activity of copper: interplay of ascorbate, cysteine, and glutathione.
2023, 15 (7), - .
DOI: 10.1093/mtomcs/mfad040
HAL: hal-04182669v1
A Jalila Simaan, Marius Réglier, Lisa Zuily, Nora Lahrach, Enrico Falcone, Merwan Bouraguba, Vincent Lebrun, Elisabeth Lojou, Marie-Thérèse Giudici-Orticoni, Peter Faller, Marianne Ilbert.
Ligands as a Tool to Tune the Toxicity of Cu on Bacteria: from Boosting to Silencing.
Copper Bioinorganic Chemistry, 2023, 1 - 44.
DOI: 10.1142/9789811269493_0001
2022
Marie Corteggiani, Nadège Bossuet-Greif, Jean-Philippe Nougayrède, Deborah Byrne, Marianne Ilbert, Sébastien Dementin, Marie-Thérèse Giudici-Orticoni, Vincent Méjean, Eric Oswald, Olivier Genest.
Uncoupling the Hsp90 and DnaK chaperone activities revealed the in vivo relevance of their collaboration in bacteria.
Proc. Natl. Acad. Sci. U.S.A., 2022, 119 (37).
DOI: 10.1073/pnas.2201779119
HAL: hal-03844063v1
Lisa Zuily, Nora Lahrach, Rosi Fassler, Olivier Genest, Peter Faller, Olivier Sénèque, Yann Denis, Marie-Pierre Castanié-Cornet, Pierre Genevaux, Ursula Jakob, Dana Reichmann, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Copper Induces Protein Aggregation, a Toxic Process Compensated by Molecular Chaperones.
mBio, 2022, 13 (2).
DOI: 10.1128/mbio.03251-21
HAL: hal-03666635v1
I. Mazurenko, T. Adachi, B. Ezraty, M. Ilbert, K. Sowa, E. Lojou.
Electrochemistry of copper efflux oxidase-like multicopper oxidases involved in copper homeostasis.
Current Opinion in Electrochemistry, 2022, 32, 100919 -.
DOI: 10.1016/j.coelec.2021.100919
HAL: hal-03489391v1
Magali Roger, Philippe Leone, Ninan Blackburn, Sam Horrell, Tadeo Moreno Chicano, Marie-Thérèse Giudici-Orticoni, Luciano A. Abriata, Greg L. Hura, Michael A. Hough, Giulano Sciara, Marianne Ilbert.
Three-dimensional structure of the single domain cupredoxin AcoP.
2022.
DOI: 10.1101/2022.03.21.484586
2021
Rosi Fassler, Lisa Zuily, Nora Lahrach, Marianne Ilbert, Dana Reichmann.
The Central Role of Redox-Regulated Switch Proteins in Bacteria.
Front. Mol. Biosci., 2021, 8.
DOI: 10.3389/fmolb.2021.706039
HAL: hal-03432179v1
Romain Clément, Xie Wang, Frédéric Biaso, Marianne Ilbert, Ievgen Mazurenko, Elisabeth Lojou.
Mutations in the coordination spheres of T1 Cu affect Cu2+-activation of the laccase from Thermus thermophilus.
Biochimie, 2021, 182, 228-237.
DOI: 10.1016/j.biochi.2021.01.006
HAL: hal-03127150v1
2020
Vivek Pratap Hitaishi, Romain Clément, Ludovica Quattrocchi, Philippe Parent, David Duché, Lisa Zuily, Marianne Ilbert, Elisabeth Lojou, Ievgen Mazurenko.
Interplay between Orientation at Electrodes and Copper Activation of Thermus thermophilus Laccase for O2 Reduction.
J. Am. Chem. Soc., 2020, 142 (3), 1394 - 1405.
DOI: 10.1021/jacs.9b11147
HAL: hal-02503732v1
2019
Xie Wang, Romain Clément, Magali Roger, Marielle Bauzan, Ievgen Mazurenko, Anne de Poulpiquet, Marianne Ilbert, Elisabeth Lojou.
Bacterial Respiratory Chain Diversity Reveals a Cytochrome c Oxidase Reducing O2 at Low Overpotentials.
J. Am. Chem. Soc., 2019, 141 (28), 11093 - 11102.
DOI: 10.1021/jacs.9b03268
HAL: hal-02160924v1
2018
Ana Carina Fernandes, Mengjiao Liu, Teresa Sorbo, Lia Christina Appold, Marianne Ilbert, Géraldine Ferracci, Fabian Kiessling, Ricardo J F Branco, Twan Lammers, Olga Iranzo.
A computational and experimental study to develop E-selectin targeted peptides for molecular imaging applications.
Future Medicinal Chemistry, 2018, 10 (23), 2695 - 2711.
DOI: 10.4155/fmc-2018-0244
HAL: hal-02132464v1
Vivek Pratap Hitaishi, Ievgen Mazurenko, Malek Harb, Romain Clément, Marion Taris, Sabine Castano, David Duché, Sophie Lecomte, Marianne Ilbert, Anne de Poulpiquet, Elisabeth Lojou.
Electrostatic-Driven Activity, Loading, Dynamics, and Stability of a Redox Enzyme on Functionalized-Gold Electrodes for Bioelectrocatalysis.
ACS Catal., 2018, 8 (12), 12004 - 12014.
DOI: 10.1021/acscatal.8b03443
HAL: hal-01994370v1
E Mileo, M Ilbert, A Barducci, P Bordes, M-P Castanié-Cornet, C Garnier, P Genevaux, R Gillet, P Goloubinoff, F Ochsenbein, G Richarme, C Iobbi-Nivol, M-T Giudici, B Gontero , O Genest.
Emerging fields in chaperone proteins: A French workshop.
Biochimie, 2018, 151, 159 - 165.
DOI: 10.1016/j.biochi.2018.06.004
HAL: hal-01863220v1
Sven C. Feifel, Kai R. Stieger, Mahdi Hejazi, Xie Wang, Marianne Ilbert, Athina Zouni, Elisabeth Lojou, Fred Lisdat.
Dihemic c4-type cytochrome acting as a surrogate electron conduit: Artificially interconnecting a photosystem I supercomplex with electrodes.
Electrochemistry Communications, 2018, 91, 49 - 53.
DOI: 10.1016/j.elecom.2018.05.006
HAL: hal-01793626v1
Xie Wang, Magali Roger, Romain Clément, Sophie Lecomte, Frédéric Biaso, Luciano A. Abriata, Pascal Mansuelle, Ievgen Mazurenko, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou, Marianne Ilbert.
Electron transfer in an acidophilic bacterium: interaction between a diheme cytochrome and a cupredoxin.
Chem. Sci., 2018, 6 (21), 4879 - 4891.
DOI: 10.1039/C8SC01615A
HAL: hal-01793582v1
2017
Magali Roger, Giuliano Sciara, Frédéric Biaso, Elisabeth Lojou, Xie Wang, Marielle Bauzan, Marie-Thérèse Giudici-Orticoni, Alejandro J. Vila, Marianne Ilbert.
Impact of copper ligand mutations on a cupredoxin with a green copper center.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2017, 1858 (5), 351 - 359.
DOI: 10.1016/j.bbabio.2017.02.007
HAL: hal-01481398v1
2016
Karen Monsalve, Ievgen Mazurenko, Cristina Gutierrez-Sanchez, Marianne Ilbert, Pascale Infossi, Stefan Frielingsdorf, Marie Thérèse Giudici-Orticoni, Oliver Lenz, Elisabeth Lojou.
Impact of Carbon Nanotube Surface Chemistry on Hydrogen Oxidation by Membrane-Bound Oxygen-Tolerant Hydrogenases.
ChemElectroChem, 2016, 3 (12), 2179 - 2188.
DOI: 10.1002/celc.201600460
HAL: hal-01406080v1
2015
Karen Monsalve, Magali Roger, Cristina Gutierrez-Sanchez, Marianne Ilbert, Serge Nitsche, Deborah Byrne-Kodjabachian, Valérie Marchi, Elisabeth Lojou.
Hydrogen bioelectrooxidation on gold nanoparticle-based electrodes modified by Aquifex aeolicus hydrogenase: Application to hydrogen/oxygen enzymatic biofuel cells.
Bioelectrochemistry, 2015, 106, 47 - 55.
DOI: 10.1016/j.bioelechem.2015.04.010
HAL: hal-01150369v1
2014
Magali Roger, Frédéric Biaso, Cindy J. Castelle, Marielle Bauzan, Florence Chaspoul, Elisabeth Lojou, Giuliano Sciara, Stefano Caffarri, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Spectroscopic Characterization of a Green Copper Site in a Single-Domain Cupredoxin.
PLoS ONE, 2014, 9 (6), 1 - 13.
DOI: 10.1371/journal.pone.0098941
HAL: hal-01494439v1
M. Roger, A. de Poulpiquet, A. Ciaccafava, M. Ilbert, M. Guiral, M. T. Giudici-Orticoni, E. Lojou.
Reconstitution of supramolecular organization involved in energy metabolism at electrochemical interfaces for biosensing and bioenergy production.
Anal Bioanal Chem, 2014, 406 (6), 1011 - 1027.
DOI: 10.1007/s00216-013-7465-1
HAL: hal-01493475v1
2013
David Redelberger, Olivier Genest, Dallel Arabet, Vincent Méjean, Marianne Ilbert, Chantal Iobbi-Nivol.
Quality control of a molybdoenzyme by the Lon protease.
2013, 21 (24), 268 - 3942.
DOI: 10.1016/j.febslet.2013.10.045
Marianne Ilbert, Violaine Bonnefoy.
Insight into the evolution of the iron oxidation pathways.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2013, 1827 (2), 161 - 175.
DOI: 10.1016/j.bbabio.2012.10.001
HAL: hal-01601795v1
2012
Magali Roger, Cindy Castelle, Marianne Guiral, Pascale Infossi, Elisabeth Lojou, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Mineral respiration under extreme acidic conditions: from a supramolecular organization to a molecular adaptation in Acidithiobacillus ferrooxidans.
2012, 40 (6), 1324 - 1329.
DOI: 10.1042/BST20120141
Clément Aussignargues, Marie-Cécile Giuliani, Pascale Infossi, Elisabeth Lojou, Marianne Guiral, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Rhodanese Functions as Sulfur Supplier for Key Enzymes in Sulfur Energy Metabolism.
J. Biol. Chem., 2012, 287 (24), 19936 - 19948.
DOI: 10.1074/jbc.M111.324863
Marianne Guiral, Laurence Prunetti, Clément Aussignargues, Alexandre Ciaccafava, Pascale Infossi, Marianne Ilbert, Elisabeth Lojou, Marie-Thérèse Giudici-Orticoni.
The Hyperthermophilic Bacterium Aquifex aeolicus: From Respiratory Pathways to Extremely Resistant Enzymes and Biotechnological Applications.
2012, 61, 125 - 194.
DOI: 10.1016/B978-0-12-394423-8.00004-4
Alexandre Ciaccafava, Pascale Infossi, Marianne Ilbert, Marianne Guiral, Sophie Lecomte, Marie Thérèse Giudici-Orticoni, Elisabeth Lojou.
Electrochemistry, AFM, and PM-IRRA Spectroscopy of Immobilized Hydrogenase: Role of a Hydrophobic Helix in Enzyme Orientation for Efficient H2 Oxidation.
Angew. Chem. Int. Ed., 2012, 51 (4), 953 - 956.
DOI: 10.1002/anie.201107053
2010
Cindy Castelle, Marianne Ilbert, Pascale Infossi, Gisèle Leroy, Marie-Thérèse Giudici-Orticoni.
An Unconventional Copper Protein Required for Cytochrome c Oxidase Respiratory Function under Extreme Acidic Conditions.
J. Biol. Chem., 2010, 1787 (28), 626 - 21525.
DOI: 10.1074/jbc.M110.131359