Maître de Conférence, HDR, Aix-Marseille Université
+33 4 91 16 45 57
Equipe / Group: BIP 07
Since many years, my research projects deal with the study of structural transitions in proteins, including Intrinsically Disordered Proteins (IDPs), flexible proteins, metalloproteins and membrane proteins. The approach I use is Site Directed Spin Labeling (SDSL), based on the use of a spin probe, and EPR spectroscopy (Electronic Paramagnetic Resonance). Spin probes can be either nitroxide molecules or metal centers (endogenous or incorporated). Distance measurements between spin probes is also an approach I use to follow structural modifications in biological systems. I am also interested to study the link of protein conformational changes to their functions, in particular electron transfer, and in original biological approaches using non natural amino acids combined with SDSL, using non commercial spin labels.
Publications
ORCID id: 0000-0001-8576-5675
2024
Maxime Bizet, Deborah Byrne, Frédéric Biaso, Guillaume Gerbaud, Emilien Etienne, Giuseppina Briola, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Tamas Kalai, Gilles Truan, Marlene Martinho.
Structural insights into the semiquinone form of human Cytochrome P450 reductase by DEER distance measurements between a native flavin and a spin labelled non‐canonical amino acid.
Chemistry Eur J, 2024.
DOI: 10.1002/chem.202304307
HAL: hal-04426634
Marlène Martinho, Diane Allegro, Emilien Etienne, Cynthia Lohberger, Alessio Bonucci, Valérie Belle, Pascale Barbier.
Structural Flexibility of Tau in Its Interaction with Microtubules as Viewed by Site-Directed Spin Labeling EPR Spectroscopy.
Methods in Molecular Biology, 2024, 2754, 55 - 75.
DOI: 10.1007/978-1-0716-3629-9_3
HAL: hal-04532622v1
Margot Di Cesare, Elise Kaplan, Julia Rendon, Guillaume Gerbaud, Sepideh Valimehr, Alexia Gobet, Thu-Anh Thi Ngo, Vincent Chaptal, Pierre Falson, Marlène Martinho, Pierre Dorlet, Eric Hanssen, Jean-Michel Jault, Cédric Orelle.
The transport activity of the multidrug ABC transporter BmrA does not require a wide separation of the nucleotide-binding domains.
Journal of Biological Chemistry, 2024, 300 (1), 105546.
DOI: 10.1016/j.jbc.2023.105546
HAL: hal-04435310
Miguel Á. Casado-Combreras, Adrián Velázquez-Campoy, Marlène Martinho, Valérie Belle, Miguel A. De la Rosa, Irene Díaz-Moreno.
Cytochrome c prompts the recruitment of its nuclear partners SET/TAF-Iβ and NPM1 into biomolecular condensates.
iScience, 2024.
DOI: 10.1016/j.isci.2024.110435
HAL: hal-04636008
2023
Emilien Etienne, Annalisa Pierro, Ketty C. Tamburrini, Alessio Bonucci, Elisabetta Mileo, Marlène Martinho, Valérie Belle.
Guidelines for the Simulations of Nitroxide X-Band cw EPR Spectra from Site-Directed Spin Labeling Experiments Using SimLabel.
Molecules, 2023, 28 (3), 1348.
DOI: 10.3390/molecules28031348
HAL: hal-03971418v1
2022
Julia Rendon, Margot Di Cesare, Alexia Godet, Guillaume Gerbaud, Emilien Etienne, Vincent Chaptal, Pierre Falson, Jean-Michel Jault, Cédric Orelle, Marlène Martinho, Pierre Dorlet.
Conformational dynamics of the ABC-transporter BmrA reveals by SDSL-EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863.
DOI: 10.1016/j.bbabio.2022.148732
Miguel Á. Casado-Combreras, Francisco Rivero-Rodríguez, Carlos A. Elena-Real, Dmitry Molodenskiy, Antonio Díaz-Quintana, Marlène Martinho, Guillaume Gerbaud, Katiuska González-Arzola, Adrián Velázquez-Campoy, Dmitri Svergun, Valérie Belle, Miguel A. De la Rosa, Irene Díaz-Moreno.
PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ.
Computational and Structural Biotechnology Journal, 2022, 20, 3695 - 3707.
DOI: 10.1016/j.csbj.2022.07.009
2019
Eugénie Fournier, Sybille Tachon, Nicholas J. Fowler, Guillaume Gerbaud, Pascal Mansuelle, Pierre Dorlet, Sam P. De Visser, Valérie Belle, A. Jalila Simaan, Marlène Martinho.
The Hunt for the Closed Conformation of the Fruit‐Ripening Enzyme 1‐Aminocyclopropane‐1‐carboxylic Oxidase: A Combined Electron Paramagnetic Resonance and Molecular Dynamics Study.
Chem. Eur. J., 2019, 25 (60), 13766-13776.
DOI: 10.1002/chem.201903003
HAL: hal-02303326v1
Pascale Barbier, Orgeta Zejneli, Marlène Martinho, Alessia Lasorsa, Valérie Belle, Caroline Smet-Nocca, Philipp O. Tsvetkov, François Devred, Isabelle Landrieu.
Role of Tau as a Microtubule-Associated Protein: Structural and Functional Aspects.
Front. Aging Neurosci., 2019, 11:204, 1 - 14.
DOI: 10.3389/fnagi.2019.00204
HAL: hal-02279660v1
Sybille Tachon, Eugénie Fournier, Christophe Decroos, Pascal Mansuelle, Emilien Etienne, Marc Maresca, Marlène Martinho, Valérie Belle, Thierry Tron, Ariane Jalila Simaan.
Chemical Modification of 1-Aminocyclopropane Carboxylic Acid (ACC) Oxidase: Cysteine Mutational Analysis, Characterization, and Bioconjugation with a Nitroxide Spin Label.
Mol Biotechnol, 2019, 61, 650 - 662.
DOI: 10.1007/s12033-019-00191-5
HAL: hal-02157289v1
Nolwenn Le Breton, Sonia Longhi, Antal Rockenbauer, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle, Marlène Martinho.
Probing the dynamic properties of two sites simultaneously in a protein–protein interaction process: a SDSL-EPR study.
Phys. Chem. Chem. Phys., 2019, 21, 22584 - 22588.
DOI: 10.1039/c9cp04660g
HAL: hal-02341622v1
Marlène Martinho, Eugénie Fournier, Nolwenn Le Breton, Elisabetta Mileo, Valérie Belle.
Nitroxide spin labels: fabulous spy spins for biostructural EPR applications.
Electron Paramagnetic Resonance, 2019, 26, 66 - 88.
DOI: 10.1039/9781788013888-00066
HAL: hal-01953977v1
Eugénie Fournier, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Valérie Belle, Marlène Martinho.
Spin labels: spies in the heart of proteins.
Actualité Chimique, 2019, 443.
(voir / see)
2018
Marlène Martinho, Diane Allegro, Isabelle Huvent, Charlotte Chabaud, Emilien Etienne, Hervé Kovacic, Bruno Guigliarelli, Vincent Peyrot, Isabelle Landrieu, Valérie Belle, Pascale Barbier.
Two Tau binding sites on tubulin revealed by thiol-disulfide exchanges.
Sci Rep, 2018, 8:13846, 1 - 11.
DOI: 10.1038/s41598-018-32096-9
HAL: hal-01883844v1
Pascal Griesmar, Marlène Martinho, Bruno Faure.
Synthèse de complexes du vanadium et leur analyse par spectroscopie d'absorption UV–visible et infra-rouge.
Bulletin de l'Union des Physiciens, 01/06/2018, 2018, 6, 1005.
2017
Nadia El Bakkali-Tahéri, Sybille Tachon, Maylis Orio, Sylvain Bertaina, Marlène Martinho, Viviane Robert, Marius Réglier, Thierry Tron, Pierre Dorlet, A. Jalila Simaan.
Characterization of Cu(II)-reconstituted ACC Oxidase using experimental and theoretical approaches.
Archives of Biochemistry and Biophysics, 2017, 623-624, 31-41.
DOI: 10.1016/j.abb.2017.03.012
HAL: hal-01627933v1
Jennifer O. Bigelow, Jason England, Johannes E. M. N. Klein, Erik R. Farquhar, Jonathan R. Frisch, Marlène Martinho, Debasish Mandal, Eckard Münck, Sason Shaik, Lawrence Que.
Oxoiron(IV) Tetramethylcyclam Complexes with Axial Carboxylate Ligands: Effect of Tethering the Carboxylate on Reactivity.
Inorg. Chem., 2017, 56 (6), 3287 - 3301.
DOI: 10.1021/acs.inorgchem.6b02659
HAL: hal-01514906v1
Emilien Etienne, Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Valérie Belle.
SimLabel: a graphical user interface to simulate continuous wave EPR spectra from site-directed spin labeling experiments.
Magn. Reson. Chem., 2017, 55 (8), 714 - 719.
DOI: 10.1002/mrc.4578
HAL: hal-01444172v1
2016
Nolwenn Le Breton, Tiona Adrianaivomananjaona, Guillaume Gerbaud, Emilien Etienne, Elena Bisetto, Alain Dautant, Bruno Guigliarelli, Francis Haraux, Marlène Martinho, Valérie Belle.
Dimerization interface and dynamic properties of yeast IF1 revealed by Site-Directed Spin Labeling EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (1), 89 - 97.
DOI: 10.1016/j.bbabio.2015.10.010
HAL: hal-01413333v1
2015
Linda Payet, Marlène Martinho, Céline Merstorf, Manuela Pastoriza-Gallego, Juan Pelta, Virgile Viasnoff, Loïc Auvray, Murugappan Muthukumar, Jérôme Mathé.
Temperature Effect on Ionic Current and ssDNA Transport through Nanopores.
Biophysical Journal, 2015, 109 (8), 1600 - 1607.
DOI: 10.1016/j.bpj.2015.08.043
HAL: hal-01444203v1
Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 - 7.
DOI: 10.3389/fmolb.2015.00021
HAL: hal-01429982v1
2014
Jason England, Jennifer O. Bigelow, Katherine M. Van Heuvelen, Erik R. Farquhar, Marlène Martinho, Katlyn K. Meier, Jonathan R. Frisch, Eckard Münck, Lawrence Que.
An ultra-stable oxoiron(IV) complex and its blue conjugate base.
Chem. Sci., 2014, 5, 1204 - 1215.
DOI: 10.1039/C3SC52755G
Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Diversification of EPR signatures in site directed spin labeling using a β-phosphorylated nitroxide.
Phys. Chem. Chem. Phys., 2014, 16, 4202 - 4209.
DOI: 10.1039/c3cp54816c
HAL: hal-01460516v1
2013
Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 - 1117.
DOI: 10.1021/bc4000542
HAL: hal-01460460v1
2012
Marlène Martinho, Johnny Habchi, Zeina El Habre, Léo Nesme, Bruno Guigliarelli, Valérie Belle, Sonia Longhi.
Assessing induced folding within the intrinsically disordered C-terminal domain of the Henipavirus nucleoproteins by site-directed spin labeling EPR spectroscopy.
J. Biomol. Struct. Dyn., 2012, 31, 453-471.
DOI: 10.1080/07391102.2012.706068
Linda Payet, Marlène Martinho, Manuela Pastoriza-Gallego, Jean-Michel Betton, Loïc Auvray, Juan Pelta, Jérôme Mathé.
Thermal Unfolding of Proteins Probed at the Single Molecule Level Using Nanopores.
Anal. Chem., 2012, 84 (9), 4071 - 4076.
DOI: 10.1021/ac300129e
Johnny Habchi, Marlène Martinho, Antoine Gruet, Bruno Guigliarelli, Sonia Longhi, Valérie Belle.
Monitoring structural transitions in IDPs by site-directed spin-labeling EPR spectroscopy.
Intrinsically Disordered Protein Analysis Volume 1, Methods and Experimental Tools, 2012, 361-386.
DOI: 10.1007/978-1-61779-927-3_21
2011
Frédéric Halgand, Johnny Habchi, Laetitia Cravello, Marlène Martinho, Bruno Guigliarelli, Sonia Longhi.
Dividing To Unveil Protein Microheterogeneities: Traveling Wave Ion Mobility Study.
Anal. Chem., 2011, 83 (19), 7306 - 7315.
DOI: 10.1021/ac200994c
HAL: hal-00677256v1
Magali Lorenzi, Carine Puppo, Régine Lebrun, Sabrina Lignon, Valérie Roubaud, Marlène Martinho, Elisabetta Mileo, Paul Tordo, Sylvain R. A. Marque, Brigitte Gontero, Bruno Guigliarelli, Valérie Belle.
Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins.
Angew. Chem. Int. Ed., 2011, 50, 9108 - 9111.
DOI: 10.1002/anie.201102539
HAL: hal-00677247v1