Chargée de Recherche, HDR, CNRS
+33 4 91 16 45 23
Equipe / Group: BIP 07
I am a chemist and a spectroscopist with experience on the synthesis and use of nitroxide radicals as spin probes and spin labels in supramolecular chemistry and Site-Directed Spin Labelling coupled to EPR spectroscopy (SDSL-EPR) applied to structural biology. I obtained my PhD in 2010 at the University of Bologna (Italy). During my PhD, I obtained a Marco Polo fellowship to visit the group of A. E. Kaifer, during 6 months (Miami, Florida) investigating the self-assembly of resorcinarene capsules using nitroxide probes and EPR spectroscopy. After a postdoctoral fellow in the group of S. Marque (ICR, Marseille, France) working on the synthesis of new nitroxide-based spin labels for selective Tyrosine labeling, I moved in the group of B. Guigliarelli at the BIP laboratory (Marseille, France) and my scientific interests moved towards the investigation of protein structural dynamics by EPR spectroscopy. In 2014, I obtained a full position as CNRS researcher (Marseille, France).
My research activities are principally focused on the structural investigation of chaperones and other flexible proteins as well as on the development of new tools and new labels to follow proteins “in action” by SDSL-EPR directly inside cells.
If you are a motivated student interested in joining the group… please apply anytime!
Publications
ORCID id: 0000-0002-0883-6043
2024
Yasmin Ben‐Ishay, Yoav Barak, Akiva Feintuch, Olivier Ouari, Annalisa Pierro, Elisabetta Mileo, Xun‐Cheng Su, Daniella Goldfarb.
Exploring the dynamics and structure of PpiB in living Escherichia coli cells using electron paramagnetic resonance spectroscopy.
Protein Science, 2024, 33 (3), e4903.
DOI: 10.1002/pro.4903
HAL: hal-04466515
Barbara Zambelli, Elisabetta Mileo.
Messages from cells: breaking new ground in deciphering protein behaviour.
Science Featured, 2024.
(voir / see)
HAL: hal-04512928
2023
Annalisa Pierro, Ketty Concetta Tamburrini, Hugo Leguenno, Guillaume Gerbaud, Emilien Etienne, Bruno Guigliarelli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR.
iScience, 2023, 26 (10), 107855.
DOI: 10.1016/j.isci.2023.107855
HAL: hal-04233485v1
Emilien Etienne, Annalisa Pierro, Ketty C. Tamburrini, Alessio Bonucci, Elisabetta Mileo, Marlène Martinho, Valérie Belle.
Guidelines for the Simulations of Nitroxide X-Band cw EPR Spectra from Site-Directed Spin Labeling Experiments Using SimLabel.
Molecules, 2023, 28 (3), 1348.
DOI: 10.3390/molecules28031348
HAL: hal-03971418v1
2022
Annalisa Pierro, Alessio Bonucci, Davide Normanno, Mireille Ansaldi, Eric Pilet, Olivier Ouari, Bruno Guigliarelli, Emilien Etienne, Guillaume Gerbaud, Axel Magalon, Valérie Belle, Elisabetta Mileo.
Probing Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide‐Based EPR Spectroscopy.
Chemistry A European J, 2022.
DOI: 10.1002/chem.202202249
HAL: hal-03836431
2021
Annalisa Pierro, Alessio Bonucci, Valérie Belle, Elisabetta Mileo.
La RPE in cellula : étudier la dynamique des protéines dans leur habitat.
Actualité Chimique, 2021, 468, 38-41.
(voir / see)
HAL: hal-03855076v1
F. Torricella, A. Pierro, E. Mileo, V. Belle, A. Bonucci.
Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies.
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2021, 1869 (7), 140653.
DOI: 10.1016/j.bbapap.2021.140653
HAL: hal-03441104v1
2020
Annalisa Pierro, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Stefano Ciurli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
Nickel and GTP Modulate Helicobacter pylori UreG Structural Flexibility.
Biomolecules, 2020, 10 (7), 1062.
DOI: 10.3390/biom10071062
HAL: hal-02902916v1
Alessio Bonucci, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo.
In‐Cell EPR: Progress towards Structural Studies Inside Cells.
ChemBioChem, 2020, 21 (4), 451 - 460.
DOI: 10.1002/cbic.201900291
HAL: hal-02180300v1
2019
Marlène Martinho, Eugénie Fournier, Nolwenn Le Breton, Elisabetta Mileo, Valérie Belle.
Nitroxide spin labels: fabulous spy spins for biostructural EPR applications.
Electron Paramagnetic Resonance, 2019, 26, 66 - 88.
DOI: 10.1039/9781788013888-00066
HAL: hal-01953977v1
Eugénie Fournier, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Valérie Belle, Marlène Martinho.
Spin labels: spies in the heart of proteins.
Actualité Chimique, 2019, 443.
(voir / see)
2018
E Mileo, M Ilbert, A Barducci, P Bordes, M-P Castanié-Cornet, C Garnier, P Genevaux, R Gillet, P Goloubinoff, F Ochsenbein, G Richarme, C Iobbi-Nivol, M-T Giudici, B Gontero , O Genest.
Emerging fields in chaperone proteins: A French workshop.
Biochimie, 2018, 151, 159 - 165.
DOI: 10.1016/j.biochi.2018.06.004
HAL: hal-01863220v1
Ganesan Karthikeyan, Alessio Bonucci, Gilles Casano, Guillaume Gerbaud, Sébastien Abel, Virginie Thomé, Laurent Kodjabachian, Axel Magalon, Bruno Guigliarelli, Valérie Belle, Olivier Ouari, Elisabetta Mileo.
A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies.
Angew. Chem. Int. Ed., 2018, 57, 1366 - 1370.
DOI: 10.1002/anie.201710184
HAL: hal-02000559v1
2017
Christoph Gmeiner, Daniel Klose, Elisabetta Mileo, Valérie Belle, Sylvain R. A. Marque, Georg Dorn, Frédéric H. T. Allain, Bruno Guigliarelli, Gunnar Jeschke, Maxim Yulikov.
Orthogonal Tyrosine and Cysteine Site-Directed Spin Labeling for Dipolar Pulse EPR Spectroscopy on Proteins.
J. Phys. Chem. Lett., 2017, 8 (19), 4852 - 4857.
DOI: 10.1021/acs.jpclett.7b02220
HAL: hal-01596569v1
Marta Palombo, Alessio Bonucci, Emilien Etienne, Stefano Ciurli, Vladimir N. Uversky, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo, Barbara Zambelli.
The relationship between folding and activity in UreG, an intrinsically disordered enzyme.
Sci Rep, 2017, 89 (1), 392.
DOI: 10.1038/s41598-017-06330-9
HAL: hal-01585289v1
Emilien Etienne, Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Valérie Belle.
SimLabel: a graphical user interface to simulate continuous wave EPR spectra from site-directed spin labeling experiments.
Magn. Reson. Chem., 2017, 55 (8), 714 - 719.
DOI: 10.1002/mrc.4578
HAL: hal-01444172v1
2015
Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 - 7.
DOI: 10.3389/fmolb.2015.00021
HAL: hal-01429982v1
2014
Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Diversification of EPR signatures in site directed spin labeling using a β-phosphorylated nitroxide.
Phys. Chem. Chem. Phys., 2014, 16, 4202 - 4209.
DOI: 10.1039/c3cp54816c
HAL: hal-01460516v1
2013
Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 - 1117.
DOI: 10.1021/bc4000542
HAL: hal-01460460v1
Elisabetta Mileo, Magali Lorenzi, Jenny Erales, Sabrina Lignon, Carine Puppo, Nolwenn Le Breton, Emilien Etienne, Sylvain R. A. Marque, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
Dynamics of the intrinsically disordered protein CP12 in its association with GAPDH in the green alga Chlamydomonas reinhardtii: a fuzzy complex.
Mol. BioSyst., 2013, 1 (11), 349.
DOI: 10.1039/c3mb70190e
HAL: hal-01460463v1
2011
Magali Lorenzi, Carine Puppo, Régine Lebrun, Sabrina Lignon, Valérie Roubaud, Marlène Martinho, Elisabetta Mileo, Paul Tordo, Sylvain R. A. Marque, Brigitte Gontero, Bruno Guigliarelli, Valérie Belle.
Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins.
Angew. Chem. Int. Ed., 2011, 50, 9108 - 9111.
DOI: 10.1002/anie.201102539
HAL: hal-00677247v1