Elisabetta MILEO

member photo

Chargée de Recherche, CNRS

+33 4 91 16 44 14

Equipe / Group: BIP 07

I am a chemist and a spectroscopist with experience on the synthesis and use of nitroxide radicals as spin probes and spin labels in supramolecular chemistry and Site-Directed Spin Labelling coupled to EPR spectroscopy (SDSL-EPR) applied to structural biology. I obtained my PhD in 2010 at the University of Bologna (Italy). During my PhD, I obtained a Marco Polo fellowship to visit the group of A. E. Kaifer, during 6 months (Miami, Florida) investigating the self-assembly of resorcinarene capsules using nitroxide probes and EPR spectroscopy. After a postdoctoral fellow in the group of S. Marque (ICR, Marseille, France) working on the synthesis of new nitroxide-based spin labels for selective Tyrosine labeling, I moved in the group of B. Guigliarelli at the BIP laboratory (Marseille, France) and my scientific interests moved towards the investigation of protein structural dynamics by EPR spectroscopy. In 2014, I obtained a full position as CNRS researcher (Marseille, France).
My research activities are principally focused on the structural investigation of chaperones and other flexible proteins as well as on the development of new tools and new labels to follow proteins “in action” by SDSL-EPR directly inside cells.
If you are a motivated student interested in joining the group… please apply anytime!

ORCID id: 0000-0002-0883-6043


Alessio Bonucci, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo.
In‐Cell EPR: Progress towards Structural Studies Inside Cells.
ChemBioChem, 2020, 21 (4), 451 - 460. DOI: 10.1002/cbic.201900291 HAL: hal-02180300v1

Annalisa Pierro, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Stefano Ciurli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
Nickel and GTP Modulate Helicobacter pylori UreG Structural Flexibility.
Biomolecules, 2020, 10 (7), 1062. DOI: 10.3390/biom10071062 HAL: hal-02902916v1


Marlène Martinho, Eugénie Fournier, Nolwenn Le Breton, Elisabetta Mileo, Valérie Belle.
Nitroxide spin labels: fabulous spy spins for biostructural EPR applications.
Electron Paramagnetic Resonance, 2019, 26, 66 - 88. DOI: 10.1039/9781788013888-00066

Eugénie Fournier, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Valérie Belle, Marlène Martinho.
Spin labels: spies in the heart of proteins.
Actualité Chimique, 2019, 443.


Ganesan Karthikeyan, Alessio Bonucci, Gilles Casano, Guillaume Gerbaud, Sébastien Abel, Virginie Thomé, Laurent Kodjabachian, Axel Magalon, Bruno Guigliarelli, Valérie Belle, Olivier Ouari, Elisabetta Mileo.
A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies.
Angew. Chem. Int. Ed., 2018, 57, 1366 - 1370. DOI: 10.1002/anie.201710184 HAL: hal-02000559v1

E Mileo, M Ilbert, A Barducci, P Bordes, M-P Castanié-Cornet, C Garnier, P Genevaux, R Gillet, P Goloubinoff, F Ochsenbein, G Richarme, C Iobbi-Nivol, M-T Giudici, B Gontero , O Genest.
Emerging fields in chaperone proteins: A French workshop.
Biochimie, 2018, 151, 159 - 165. DOI: 10.1016/j.biochi.2018.06.004 HAL: hal-01863220v1


Christoph Gmeiner, Daniel Klose, Elisabetta Mileo, Valérie Belle, Sylvain R. A. Marque, Georg Dorn, Frédéric H. T. Allain, Bruno Guigliarelli, Gunnar Jeschke, Maxim Yulikov.
Orthogonal Tyrosine and Cysteine Site-Directed Spin Labeling for Dipolar Pulse EPR Spectroscopy on Proteins.
J. Phys. Chem. Lett., 2017, 8 (19), 4852 - 4857. DOI: 10.1021/acs.jpclett.7b02220 HAL: hal-01596569v1

Emilien Etienne, Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Valérie Belle.
SimLabel: a graphical user interface to simulate continuous wave EPR spectra from site-directed spin labeling experiments.
Magn. Reson. Chem., 2017, 55 (8), 714 - 719. DOI: 10.1002/mrc.4578 HAL: hal-01444172v1

Marta Palombo, Alessio Bonucci, Emilien Etienne, Stefano Ciurli, Vladimir N. Uversky, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo, Barbara Zambelli.
The relationship between folding and activity in UreG, an intrinsically disordered enzyme.
Sci Rep, 2017, 89 (1), 392 -. DOI: 10.1038/s41598-017-06330-9 HAL: hal-01585289v1


Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 - 7. DOI: 10.3389/fmolb.2015.00021 HAL: hal-01429982v1


Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Diversification of EPR signatures in site directed spin labeling using a β-phosphorylated nitroxide.
Phys. Chem. Chem. Phys., 2014, 16, 4202 - 4209. DOI: 10.1039/c3cp54816c HAL: hal-01460516v1


Elisabetta Mileo, Magali Lorenzi, Jenny Erales, Sabrina Lignon, Carine Puppo, Nolwenn Le Breton, Emilien Etienne, Sylvain R. A. Marque, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
Dynamics of the intrinsically disordered protein CP12 in its association with GAPDH in the green alga Chlamydomonas reinhardtii: a fuzzy complex.
Mol. BioSyst., 2013, 1 (11), 349 -. DOI: 10.1039/c3mb70190e HAL: hal-01460463v1

Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 - 1117. DOI: 10.1021/bc4000542 HAL: hal-01460460v1


Magali Lorenzi, Carine Puppo, Régine Lebrun, Sabrina Lignon, Valérie Roubaud, Marlène Martinho, Elisabetta Mileo, Paul Tordo, Sylvain R. A. Marque, Brigitte Gontero, Bruno Guigliarelli, Valérie Belle.
Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins.
Angew. Chem. Int. Ed., 2011, 50, 9108 - 9111. DOI: 10.1002/anie.201102539