Directeur de Recherche, CNRS
+33 4 91 16 46 53
Equipe / Group: BIP 01
Chaperone proteins – Molecular microbiology – Bacterial stress response – Hsp90/HtpG – Hsp70/DnaK.
Le but principal de mes recherches est de comprendre comment les protéines chaperons participent à l’adaptation des bactéries en conditions de stress. Présentes des bactéries à l’homme, les protéines chaperons sont essentielles pour permettre le repliement correct de la plupart des autres protéines cellulaires. Par des approches de microbiologie moléculaire et de biochimie, je m’intéresse tout particulièrement au chaperon Hsp90 et au système chaperon DnaK chez des bactéries modèles telles que Shewanella oneidensis et Escherichia coli.
The main goal of my research is to understand how chaperone proteins are involved in bacterial adaptation under stress conditions. Present from bacteria to human, chaperone proteins are essential to allow the correct folding of most of the cellular proteins. Using molecular microbiology and biochemical approaches, I mainly focus on the Hsp90 chaperone and the Hsp70 chaperone system in model bacteria including Shewanella oneidensis and Escherichia coli.
Publications
ORCID id: 0000-0003-1240-2827
2023
Lana Weber, Atar Gilat, Nathanael Maillot, Deborah Byrne, Pascal Arnoux, Marie‐Thérèse Giudici‐Orticoni, Vincent Méjean, Marianne Ilbert, Olivier Genest, Rina Rosenzweig, Sébastien Dementin.
Bacterial adaptation to cold: Conservation of a short J‐domain co‐chaperone and its protein partners in environmental proteobacteria.
Environmental Microbiology, 2023.
DOI: 10.1111/1462-2920.16478
2022
Marie Corteggiani, Nadège Bossuet-Greif, Jean-Philippe Nougayrède, Deborah Byrne, Marianne Ilbert, Sébastien Dementin, Marie-Thérèse Giudici-Orticoni, Vincent Méjean, Eric Oswald, Olivier Genest.
Uncoupling the Hsp90 and DnaK chaperone activities revealed the in vivo relevance of their collaboration in bacteria.
Proc. Natl. Acad. Sci. U.S.A., 2022, 119 (37).
DOI: 10.1073/pnas.2201779119
HAL: hal-03844063v1
Lisa Zuily, Nora Lahrach, Rosi Fassler, Olivier Genest, Peter Faller, Olivier Sénèque, Yann Denis, Marie-Pierre Castanié-Cornet, Pierre Genevaux, Ursula Jakob, Dana Reichmann, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Copper Induces Protein Aggregation, a Toxic Process Compensated by Molecular Chaperones.
mBio, 2022, 13 (2).
DOI: 10.1128/mbio.03251-21
HAL: hal-03666635v1
2021
Sue Wickner, Thu-Lan Lily Nguyen, Olivier Genest.
The Bacterial Hsp90 Chaperone: Cellular Functions and Mechanism of Action.
Annu. Rev. Microbiol., 2021, 75 (1).
DOI: 10.1146/annurev-micro-032421-035644
Patricija van Oosten-Hawle, Steven Bergink, Brian Blagg, Jeff Brodsky, Adrienne Edkins, Brian Freeman, Olivier Genest, Linda Hendershot, Harm Kampinga, Jill Johnson, Antonio De Maio, Dan Masison, Kevin Morano, Gabriele Multhoff, Chris Prodromou, Veena Prahlad, Ruth Scherz-Shouval, Anastasia Zhuravleva, Mehdi Mollapour, Andrew W. Truman.
First Virtual International Congress on Cellular and Organismal Stress Responses, November 5–6, 2020.
Cell Stress and Chaperones, 2021, 26 (2), 289 - 295.
DOI: 10.1007/s12192-021-01192-7
HAL: hal-03367646v1
Nathanael Jean Maillot, Pascale Infossi, Sébastien Dementin, Marie-Thérèse Giudici-Orticoni, Vincent Méjean, Olivier Genest.
Modulation of the RNA polymerase activity by AtcB, a protein associated with a DnaK chaperone network in Shewanella oneidensis.
Biochemical and Biophysical Research Communications, 2021, 535, 66 - 72.
DOI: 10.1016/j.bbrc.2020.12.015
HAL: hal-03116745v1
2019
Nathanael Jean Maillot, Flora Ambre Honoré, Deborah Byrne, Vincent Méjean, Olivier Genest.
Cold adaptation in the environmental bacterium Shewanella oneidensis is controlled by a J-domain co-chaperone protein network.
Commun Biol, 2019, 2 (1).
DOI: 10.1038/s42003-019-0567-3
HAL: hal-02371718v1
Flora Ambre Honoré, Nathanael Jean Maillot, Vincent Méjean, Olivier Genest.
Interplay between the Hsp90 Chaperone and the HslVU Protease To Regulate the Level of an Essential Protein in Shewanella oneidensis.
mBio, 2019, 78 (3), 219 -.
DOI: 10.1128/mBio.00269-19
HAL: hal-02180326v1
Laura J. Blair, Olivier Genest, Mehdi Mollapour.
The multiple facets of the Hsp90 machine.
Nat Struct Mol Biol, 2019, 24 (2), 2013 - 95.
DOI: 10.1038/s41594-018-0177-7
HAL: hal-02273116v1
Olivier Genest, Sue Wickner, Shannon M. Doyle.
Hsp90 and Hsp70 chaperones: Collaborators in protein remodeling.
J. Biol. Chem., 2019, 745 (6), 1 - 2120.
DOI: 10.1074/jbc.REV118.002806
HAL: hal-02273131v1
2018
E Mileo, M Ilbert, A Barducci, P Bordes, M-P Castanié-Cornet, C Garnier, P Genevaux, R Gillet, P Goloubinoff, F Ochsenbein, G Richarme, C Iobbi-Nivol, M-T Giudici, B Gontero , O Genest.
Emerging fields in chaperone proteins: A French workshop.
Biochimie, 2018, 151, 159 - 165.
DOI: 10.1016/j.biochi.2018.06.004
HAL: hal-01863220v1
Andrea N. Kravats, Joel R. Hoskins, Michael Reidy, Jill L. Johnson, Shannon M. Doyle, Olivier Genest, Daniel C. Masison, Sue Wickner.
Functional and physical interaction between yeast Hsp90 and Hsp70.
Proc Natl Acad Sci USA, 2018, 67 (10), 947 - E2219.
DOI: 10.1073/pnas.1719969115
HAL: hal-01890402v1
2017
Sophie Bouillet, Olivier Genest, Vincent Méjean, Chantal Iobbi-Nivol.
Protection of the general stress response σ S factor by the CrsR regulator allows a rapid and efficient adaptation of Shewanella oneidensis.
J. Biol. Chem., 2017, 36 (36), 410 - 14928.
DOI: 10.1074/jbc.M117.781443
HAL: hal-01696646v1
Flora Ambre Honoré, Vincent Méjean, Olivier Genest.
Hsp90 Is Essential under Heat Stress in the Bacterium Shewanella oneidensis.
Cell Reports, 2017, 19 (4), 680 - 687.
DOI: 10.1016/j.celrep.2017.03.082
HAL: hal-01525196v1
Andrea N. Kravats, Shannon M. Doyle, Joel R. Hoskins, Olivier Genest, Erin Doody, Sue Wickner.
Interaction of E. coli Hsp90 with DnaK Involves the DnaJ Binding Region of DnaK.
Journal of Molecular Biology, 2017, 429 (6), 858 - 872.
DOI: 10.1016/j.jmb.2016.12.014
HAL: hal-01697476v1
Olivier N. Lemaire, Sophie Bouillet, Vincent Méjean, Chantal Iobbi-Nivol, Olivier Genest.
Chaperones in maturation of molybdoenzymes: Why specific is better than general?.
Bioengineered, 2017, 8 (2), 133 - 136.
DOI: 10.1080/21655979.2016.1218579
HAL: hal-01413222v1
2016
Sophie Bouillet, Olivier Genest, Cécile Jourlin-Castelli, Michel Fons, Vincent Méjean, Chantal Iobbi-Nivol.
The General Stress Response σ S Is Regulated by a Partner Switch in the Gram-negative Bacterium Shewanella oneidensis.
J. Biol. Chem., 2016, 49 (50), 603 - 26163.
DOI: 10.1074/jbc.M116.751933
HAL: hal-01415795v1
2015
Olivier Genest, Joel R. Hoskins, Andrea N. Kravats, Shannon M. Doyle, Sue Wickner.
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in Protein Remodeling.
Journal of Molecular Biology, 2015, 427 (24), 3877 - 3889.
DOI: 10.1016/j.jmb.2015.10.010
HAL: hal-01432234v1
2013
David Redelberger, Olivier Genest, Dallel Arabet, Vincent Méjean, Marianne Ilbert, Chantal Iobbi-Nivol.
Quality control of a molybdoenzyme by the Lon protease.
2013, 21 (24), 268 - 3942.
DOI: 10.1016/j.febslet.2013.10.045
Shannon M. Doyle, Olivier Genest, Sue Wickner.
Protein rescue from aggregates by powerful molecular chaperone machines.
Nat Rev Mol Cell Biol, 2013, 103 (10), 6166 - 629.
DOI: 10.1038/nrm3660
Olivier Genest, Michael Reidy, Timothy O. Street, Joel R. Hoskins, Jodi L. Camberg, David A. Agard, Daniel C. Masison, Sue Wickner.
Uncovering a Region of Heat Shock Protein 90 Important for Client Binding in E. coli and Chaperone Function in Yeast.
Molecular Cell, 2013, 49 (3), 464 - 473.
DOI: 10.1016/j.molcel.2012.11.017