Eric PILET

member photo

Maître de Conférence, Aix-Marseille Université

+33 4 91 16 45 67

Equipe / Group: BIP 07

Bioenergetics deals with the energy conversion especially at the membrane level of mitochondria, chloroplast and all procaryotes. My research interests are about the last component of the respiratory chain both aerobic and anaerobic. I try to understand how those membrane complexes work: specificity of electron donors (quinone interaction), how electron shuttles throw cofactors (organic or metallo-organic), and the interaction with substrate/product/inhibitors by using various approaches: enzymology on membrane vesicles or purified enzymes, Clark electrode, redox titration followed by UV-vis spectroscopy as well as EPR spectroscopy. Currently I am working on the nitrate reductase from E. coli and the terminal oxidases from D. fructosovorans.
You can find a summary of my CV and my exhaustive list of publications by following the ORCID ID link below
Publications
ORCID id: 0000-0003-1974-4408

2022

Annalisa Pierro, Alessio Bonucci, Davide Normanno, Mireille Ansaldi, Eric Pilet, Olivier Ouari, Bruno Guigliarelli, Emilien Etienne, Guillaume Gerbaud, Axel Magalon, Valérie Belle, Elisabetta Mileo.
Probing Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide‐Based EPR Spectroscopy.
Chemistry A European J, 2022. DOI: 10.1002/chem.202202249 HAL: hal-03836431

Maryam Seif-Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
Comparative analysis of the mena- and demethylmena-(semi)quinone binding mode to the quinol oxidation site of E. coli nitrate reductase A.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, 148750. DOI: 10.1016/j.bbabio.2022.148750

Arlette Kpebe, Stéphane Grimaldi, Myriam Brugna, Eric Pilet.
First steps in the characterization of cytochrome bd oxidase from Solidesulfovibrio fructosovorans.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, 148691. DOI: 10.1016/j.bbabio.2022.148691

Eric Pilet, Maryam Seif-Eddine, Farida Seduk, Julia Rendon, Bruno Guigliarelli, Stéphane Grimaldi, Axel Magalon.
New insights in quinone reactivity in the nitrate reductase A from E. coli.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2022, 1863, 148814. DOI: 10.1016/j.bbabio.2022.148814


2020

Maryam Seif Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
1,2H hyperfine spectroscopy and DFT modeling unveil the demethylmenasemiquinone binding mode to E. coli nitrate reductase A (NarGHI).
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2020, 1861 (8), 148203. DOI: 10.1016/j.bbabio.2020.148203 HAL: hal-02909846


2017

Maryam Seif Eddine, Frédéric Biaso, Rodrigo Arias‐Cartin, Eric Pilet, Julia Rendon, Sevdalina Lyubenova, Farida Seduk, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
Probing the Menasemiquinone Binding Mode to Nitrate Reductase A by Selective 2 H and 15 N Labeling, HYSCORE Spectroscopy, and DFT Modeling.
ChemPhysChem, 2017, 18 (19), 2704 - 2714. DOI: 10.1002/cphc.201700571 HAL: hal-01635739v1


2015

Julia Rendon, Eric Pilet, Zeinab Fahs, Farida Seduk, Léa Sylvi, Mahmoud Hajj Chehade, Fabien Pierrel, Bruno Guigliarelli, Axel Magalon, Stephane Grimaldi.
Demethylmenaquinol is a substrate of Escherichia coli nitrate reductase A (NarGHI) and forms a stable semiquinone intermediate at the NarGHI quinol oxidation site.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2015, 1847 (8), 739 - 747. DOI: 10.1016/j.bbabio.2015.05.001 HAL: hal-01429032v1


2007

Eric Pilet, Wolfgang Nitschke, Ursula Liebl, Marten H. Vos.
Accommodation of NO in the active site of mammalian and bacterial cytochrome c oxidase aa3.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2007, 1767 (5), 387 - 392. DOI: 10.1016/j.bbabio.2007.03.001 HAL: hal-00324353v1


2004

Eric Pilet, Wolfgang Nitschke, Fabrice Rappaport, Tewfik Soulimane, Jean-Christophe Lambry, Ursula Liebl, Marten H. Vos.
NO Binding and Dynamics in Reduced Heme−Copper Oxidases aa 3 from Paracoccus denitrificans and ba 3 from Thermus thermophilus.
Biochemistry, 2004, 43 (44), 14118 - 14127. DOI: 10.1021/bi0488808 HAL: hal-00836426v1