Valérie BELLE

member photo

Professeure, HDR, Aix-Marseille Université

+33 4 91 16 44 14

Equipe / Group: BIP 07

The central point of my research activities is the development and application of Magnetic Resonance spectroscopies to address biological questions. I obtained my PhD in Physics in 1995 at the University of Grenoble followed by a postdoctoral stay at the University of Würzburg (Germany), working on methodological aspects of functional and perfusion Magnetic Resonance Imaging. In 1998, I obtained an assistant professor position at Aix-Marseille University and joined the EPR group of the BIP laboratory where I became professor in 2011. Being part of the Physics department, I am teaching general physics from B. Sc. and M. Sc. students. In the last two decades, I focused my research activities on the study of protein dynamics using Site Directed Spin Labeling combined with Electron Paramagnetic Resonance (SDSL-EPR) spectroscopy. I am interested in characterizing structural transitions within proteins mostly arising from protein-protein interaction, in particular in the family of intrinsically disordered proteins. I am also involved in the development of new paramagnetic spin labels (nitroxide derivatives) aiming at enlarging the potentialities of SDSL-EPR.



Alessio Bonucci, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo.
In‐Cell EPR: Progress towards Structural Studies Inside Cells.
ChemBioChem, 2020, 21 (4), 451 - 460. DOI: 10.1002/cbic.201900291 HAL: hal-02180300v1

Annalisa Pierro, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Stefano Ciurli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
Nickel and GTP Modulate Helicobacter pylori UreG Structural Flexibility.
Biomolecules, 2020, 10 (7), 1062. DOI: 10.3390/biom10071062 HAL: hal-02902916v1


Sybille Tachon, Eugénie Fournier, Christophe Decroos, Pascal Mansuelle, Emilien Etienne, Marc Maresca, Marlène Martinho, Valérie Belle, Thierry Tron, Ariane Jalila Simaan.
Chemical Modification of 1-Aminocyclopropane Carboxylic Acid (ACC) Oxidase: Cysteine Mutational Analysis, Characterization, and Bioconjugation with a Nitroxide Spin Label.
Mol Biotechnol, 2019, 61, 650 - 662. DOI: 10.1007/s12033-019-00191-5 HAL: hal-02157289v1

Marlène Martinho, Eugénie Fournier, Nolwenn Le Breton, Elisabetta Mileo, Valérie Belle.
Nitroxide spin labels: fabulous spy spins for biostructural EPR applications.
Electron Paramagnetic Resonance, 2019, 26, 66 - 88. DOI: 10.1039/9781788013888-00066

Nolwenn Le Breton, Sonia Longhi, Antal Rockenbauer, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle, Marlène Martinho.
Probing the dynamic properties of two sites simultaneously in a protein–protein interaction process: a SDSL-EPR study.
Phys. Chem. Chem. Phys., 2019, 21, 22584 - 22588. DOI: 10.1039/c9cp04660g HAL: hal-02341622v1

Pascale Barbier, Orgeta Zejneli, Marlène Martinho, Alessia Lasorsa, Valérie Belle, Caroline Smet-Nocca, Philipp O. Tsvetkov, François Devred, Isabelle Landrieu.
Role of Tau as a Microtubule-Associated Protein: Structural and Functional Aspects.
Front. Aging Neurosci., 2019, 11:204, 1 - 14. DOI: 10.3389/fnagi.2019.00204 HAL: hal-02279660v1

Eugénie Fournier, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Valérie Belle, Marlène Martinho.
Spin labels: spies in the heart of proteins.
Actualité Chimique, 2019, 443.

Eugénie Fournier, Sybille Tachon, Nicholas J. Fowler, Guillaume Gerbaud, Pascal Mansuelle, Pierre Dorlet, Sam P. De Visser, Valérie Belle, A. Jalila Simaan, Marlène Martinho.
The Hunt for the Closed Conformation of the Fruit‐Ripening Enzyme 1‐Aminocyclopropane‐1‐carboxylic Oxidase: A Combined Electron Paramagnetic Resonance and Molecular Dynamics Study.
Chem. Eur. J., 2019, 25 (60), 13766-13776. DOI: 10.1002/chem.201903003 HAL: hal-02303326v1


Ganesan Karthikeyan, Alessio Bonucci, Gilles Casano, Guillaume Gerbaud, Sébastien Abel, Virginie Thomé, Laurent Kodjabachian, Axel Magalon, Bruno Guigliarelli, Valérie Belle, Olivier Ouari, Elisabetta Mileo.
A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies.
Angew. Chem. Int. Ed., 2018, 57, 1366 - 1370. DOI: 10.1002/anie.201710184 HAL: hal-02000559v1

Angeliki M. Andrianaki, Irene Kyrmizi, Kalliopi Thanopoulou, Clara Baldin, Elias Drakos, Sameh S. M. Soliman, Amol C. Shetty, Carrie McCracken, Tonia Akoumianaki, Kostas Stylianou, Petros Ioannou, Charalampos Pontikoglou, Helen A. Papadaki, Maria Tzardi, Valerie Belle, Emilien Etienne, Anne Beauvais, George Samonis, Dimitrios P. Kontoyiannis, Evangelos Andreakos, Vincent M. Bruno, Ashraf S. Ibrahim, Georgios Chamilos.
Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species.
Nat Commun, 2018, 11 (Suppl. 1), R106 -. DOI: 10.1038/s41467-018-05820-2 HAL: hal-01892640v1

Marlène Martinho, Diane Allegro, Isabelle Huvent, Charlotte Chabaud, Emilien Etienne, Hervé Kovacic, Bruno Guigliarelli, Vincent Peyrot, Isabelle Landrieu, Valérie Belle, Pascale Barbier.
Two Tau binding sites on tubulin revealed by thiol-disulfide exchanges.
Sci Rep, 2018, 8:13846, 1 - 11. DOI: 10.1038/s41598-018-32096-9 HAL: hal-01883844v1


Christoph Gmeiner, Daniel Klose, Elisabetta Mileo, Valérie Belle, Sylvain R. A. Marque, Georg Dorn, Frédéric H. T. Allain, Bruno Guigliarelli, Gunnar Jeschke, Maxim Yulikov.
Orthogonal Tyrosine and Cysteine Site-Directed Spin Labeling for Dipolar Pulse EPR Spectroscopy on Proteins.
J. Phys. Chem. Lett., 2017, 8 (19), 4852 - 4857. DOI: 10.1021/acs.jpclett.7b02220 HAL: hal-01596569v1

Emilien Etienne, Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Valérie Belle.
SimLabel: a graphical user interface to simulate continuous wave EPR spectra from site-directed spin labeling experiments.
Magn. Reson. Chem., 2017, 55 (8), 714 - 719. DOI: 10.1002/mrc.4578 HAL: hal-01444172v1

Marta Palombo, Alessio Bonucci, Emilien Etienne, Stefano Ciurli, Vladimir N. Uversky, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo, Barbara Zambelli.
The relationship between folding and activity in UreG, an intrinsically disordered enzyme.
Sci Rep, 2017, 89 (1), 392 -. DOI: 10.1038/s41598-017-06330-9 HAL: hal-01585289v1


Nolwenn Le Breton, Tiona Adrianaivomananjaona, Guillaume Gerbaud, Emilien Etienne, Elena Bisetto, Alain Dautant, Bruno Guigliarelli, Francis Haraux, Marlène Martinho, Valérie Belle.
Dimerization interface and dynamic properties of yeast IF1 revealed by Site-Directed Spin Labeling EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2016, 1857 (1), 89 - 97. DOI: 10.1016/j.bbabio.2015.10.010 HAL: hal-01413333v1


Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 - 7. DOI: 10.3389/fmolb.2015.00021 HAL: hal-01429982v1


Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Diversification of EPR signatures in site directed spin labeling using a β-phosphorylated nitroxide.
Phys. Chem. Chem. Phys., 2014, 16, 4202 - 4209. DOI: 10.1039/c3cp54816c HAL: hal-01460516v1

Patrick Bertrand (ed).
Electron Paramagnetic Resonance Spectroscopy: Applications.
Electron Paramagnetic Resonance Spectroscopy: Applications, 2014. DOI: 10.1007/978-3-030-39668-8


Elisabetta Mileo, Magali Lorenzi, Jenny Erales, Sabrina Lignon, Carine Puppo, Nolwenn Le Breton, Emilien Etienne, Sylvain R. A. Marque, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
Dynamics of the intrinsically disordered protein CP12 in its association with GAPDH in the green alga Chlamydomonas reinhardtii: a fuzzy complex.
Mol. BioSyst., 2013, 1 (11), 349 -. DOI: 10.1039/c3mb70190e HAL: hal-01460463v1

Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 - 1117. DOI: 10.1021/bc4000542 HAL: hal-01460460v1


Marlène Martinho, Johnny Habchi, Zeina El Habre, Léo Nesme, Bruno Guigliarelli, Valérie Belle, Sonia Longhi.
Assessing induced folding within the intrinsically disordered C-terminal domain of the Henipavirus nucleoproteins by site-directed spin labeling EPR spectroscopy.
J. Biomol. Struct. Dyn., 2012, 31, 453-471. DOI: 10.1080/07391102.2012.706068

Johnny Habchi, Marlène Martinho, Antoine Gruet, Bruno Guigliarelli, Sonia Longhi, Valérie Belle.
Monitoring structural transitions in IDPs by site-directed spin-labeling EPR spectroscopy.
Intrinsically Disordered Protein Analysis Volume 1, Methods and Experimental Tools, 2012, 361-386. DOI: 10.1007/978-1-61779-927-3_21


Sonia Longhi, Valérie Belle, André Fournel, Bruno Guigliarelli, Frédéric Carrière.
Probing structural transitions in both structured and disordered proteins using site-directed spin-labeling EPR spectroscopy.
J. Peptide Sci., 2011, 106 (5), 21637 - 328. DOI: 10.1002/psc.1344

Magali Lorenzi, Carine Puppo, Régine Lebrun, Sabrina Lignon, Valérie Roubaud, Marlène Martinho, Elisabetta Mileo, Paul Tordo, Sylvain R. A. Marque, Brigitte Gontero, Bruno Guigliarelli, Valérie Belle.
Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins.
Angew. Chem. Int. Ed., 2011, 50, 9108 - 9111. DOI: 10.1002/anie.201102539


Jenny Erales, Magali Lorenzi, Régine Lebrun, André Fournel, Emilien Etienne, Carine Courcelle, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
A New Function of GAPDH from Chlamydomonas reinhardtii : A Thiol−Disulfide Exchange Reaction with CP12.
Biochemistry, 2009, 48 (25), 6034 - 6040. DOI: 10.1021/bi900569h


Valérie Belle, Sabrina Rouger, Stéphanie Costanzo, Elodie Liquière, Janez Strancar, Bruno Guigliarelli, André Fournel, Sonia Longhi.
Mapping α-helical induced folding within the intrinsically disordered C-terminal domain of the measles virus nucleoprotein by site-directed spin-labeling EPR spectroscopy.
Proteins, 2008, 73 (4), 973 - 988. DOI: 10.1002/prot.22125


Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 - 5218. DOI: 10.1021/ja060233b HAL: hal-00335157v1

Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties.
International Journal of Hydrogen Energy, 2006, 31 (11), 1424 - 1431. DOI: 10.1016/j.ijhydene.2006.06.007 HAL: hal-00335435