Métabolisme de l'hydrogène et ses enzymes
Hydrogen metabolism and its enzymesPublications
2024
Lars J.C. Jeuken, Dennis G.H. Hetterscheid, Marc T.M. Koper, Carla Casadevall, Christophe Léger, Antoni Llobet, Ross D. Milton, Ryuhei Nakamura, Kristina Tschulik.
Toward an informative comparison of heterogeneous, synthetic, and biological electrocatalysis in energy conversion.
Chem Catalysis, 2024, 101098 – .
DOI: 10.1016/j.checat.2024.101098
HAL: hal-04691332v1
Andrea Fasano, Aurore Jacq-Bailly, Jeremy Wozniak, Vincent Fourmond, Christophe Léger.
Catalytic Bias and Redox-Driven Inactivation of the Group B FeFe Hydrogenase CpIII.
ACS Catal., 2024, 14 (9), 7001 – 7010.
DOI: 10.1021/acscatal.4c01352
HAL: hal-04570861v1
(dataset available here)
Andrea Fasano, Vincent Fourmond, Christophe Léger.
Outer-sphere effects on the O2 sensitivity, catalytic bias and catalytic reversibility of hydrogenases.
Chem. Sci., 2024, 15, 5418 – 5433.
DOI: 10.1039/D4SC00691G
HAL: hal-04550524v1
(dataset available here)
Anna Aldinio-Colbachini, Alain Grossi, Américo G. Duarte, Jean-Vincent Daurelle, Vincent Fourmond.
Combining a Commercial Mixer with a Wall-Tube Electrode Allows the Arbitrary Control of Concentrations in Protein Film Electrochemistry.
Anal. Chem., 2024, 96 (12), 4868–4875.
DOI: 10.1021/acs.analchem.3c05293
HAL: hal-04510311v1
Andrea Fasano, Vincent Fourmond, Christophe Léger.
The difference bidirectionality makes to the kinetic modeling of molecular catalysis.
Curr. Op. Electrochem., 2024, 46, 101489.
DOI: 10.1016/j.coelec.2024.101489
HAL: hal-04550686v1
Xavier Marbehan, Magali Roger, Frantz Fournier, Pascale Infossi, Emmanuel Guedon, Louis Delecourt, Régine Lebrun, Marie-Thérèse Giudici-Orticoni, Stéphane Delaunay.
Combining metabolic flux analysis with proteomics to shed light on the metabolic flexibility: the case of Desulfovibrio vulgaris Hildenborough.
Front. Microbiol., 2024, 15.
DOI: 10.3389/fmicb.2024.1336360
HAL: hal-04565765
Andrea Fasano, Carole Baffert, Conrad Schumann, Gustav Berggren, James A. Birrell, Vincent Fourmond, Christophe Léger.
Kinetic Modeling of the Reversible or Irreversible Electrochemical Responses of FeFe-Hydrogenases.
J. Am. Chem. Soc., 2024, 146 (2), 1455–1466.
DOI: 10.1021/jacs.3c10693
HAL: hal-04372676v1
(dataset available here)
Andrea Fasano, Vincent Fourmond, Christophe Léger.
Kinetic modeling of 2e-/1H+ and 2e-/2H+ bidirectional catalytic cycles.
Bioelectrochemistry, 2024, 108511 –.
DOI: 10.1016/j.bioelechem.2023.108511
HAL: hal-04284492
2023
Andrea Fasano, Chloé Guendon, Aurore Jacq-Bailly, Arlette Kpebe, Jérémy Wozniak, Carole Baffert, Melisa del Barrio, Vincent Fourmond, Myriam Brugna, Christophe Léger.
A Chimeric NiFe Hydrogenase Heterodimer to Assess the Role of the Electron Transfer Chain in Tuning the Enzyme’s Catalytic Bias and Oxygen Tolerance.
J. Am. Chem. Soc., 2023, 145 (36), 20021–20030.
DOI: 10.1021/jacs.3c06895
HAL: hal-04284474
(dataset available here)
Raphaël J. Labidi, Bruno Faivre, Philippe Carpentier, Giulia Veronesi, Albert Solé-Daura, Ragnar Bjornsson, Christophe Léger, Philipp Gotico, Yun Li, Mohamed Atta, Marc Fontecave.
Light-Driven Hydrogen Evolution Reaction Catalyzed by a Molybdenum–Copper Artificial Hydrogenase.
J. Am. Chem. Soc., 2023.
DOI: 10.1021/jacs.3c01350
HAL: hal-04128647
Anna Aldinio-Colbachini, Andrea Fasano, Chloé Guendon, Aurore Bailly, Jeremy Wozniak, Carole Baffert, Arlette Kpebe, Christophe Léger, Myriam Brugna, Vincent Fourmond.
Transport limited adsorption experiments give a new lower estimate of the turnover frequency of Escherichia coli hydrogenase 1.
BBA Advances, 2023, 100090 –.
DOI: 10.1016/j.bbadva.2023.100090
HAL: hal-04221197
Tetyana Kyrpel, Vita Saska, Anne de Poulpiquet, Mathieu Luglia, Audrey Soric, Magali Roger, Oksana Tananaiko, Marie Thérèse Giudici-Orticoni, Elisabeth Lojou, Ievgen Mazurenko.
Hydrogenase-based electrode for hydrogen sensing in a fermentation bioreactor.
Biosensors and Bioelectronics, 2023, 225, 115106.
DOI: 10.1016/j.bios.2023.115106
HAL: hal-03963086
Kpebe A., Guendon C., Payne N., Ros J., Berbar M.K., Lebrun R, Baffert C., Shintu L., Brugna M.
An essential role of the reversible electron-bifurcating hydrogenase Hnd for ethanol oxidation in Solidesulfovibrio fructosivorans..
Front. Microbiol., 2023, 14.
DOI: 10.3389/fmicb.2023.1139276
HAL: hal-04071158v1
2022
Andreas Rutz, Chandan K. Das, Andrea Fasano, Jan Jaenecke, Shanika Yadav, Ulf-Peter Apfel, Vera Engelbrecht, Vincent Fourmond, Christophe Léger, Lars V. Schäfer, Thomas Happe.
Increasing the O2 Resistance of the [FeFe]-Hydrogenase CbA5H through Enhanced Protein Flexibility.
ACS Catal., 2022, 856 – 865.
DOI: 10.1021/acscatal.2c04031
HAL: hal-03932821
Natalie Payne, Arlette Kpebe, Chloé Guendon, Carole Baffert, Julien Ros, Régine Lebrun, Yann Denis, Laetitia Shintu, Myriam Brugna.
The electron-bifurcating FeFe-hydrogenase Hnd is involved in ethanol metabolism in Desulfovibrio fructosovorans grown on pyruvate.
Mol Microbiol, 2022, 117 (4), 907-920.
DOI: doi: 10.1111/mmi.14881
HAL: hal-03548887
Sven T. Stripp, Benjamin R. Duffus, Vincent Fourmond, Christophe Léger, Silke Leimkühler, Shun Hirota, Yilin Hu, Andrew Jasniewski, Hideaki Ogata, Markus W. Ribbe.
Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.
Chem. Rev., 2022, 122 (14), 11900 – 11973.
DOI: 10.1021/acs.chemrev.1c00914
HAL: hal-03741456
2021
Christina Felbek, Federica Arrigoni, David de Sancho, Aurore Jacq-Bailly, Robert B. Best, Vincent Fourmond, Luca Bertini, Christophe Léger.
Mechanism of Hydrogen Sulfide-Dependent Inhibition of FeFe Hydrogenase.
ACS Catal., 2021, 15162 – 15176.
DOI: 10.1021/acscatal.1c04838
HAL: hal-03468382
Andrea Fasano, Henrik Land, Vincent Fourmond, Gustav Berggren, Christophe Léger.
Reversible or Irreversible Catalysis of H+/H2 Conversion by FeFe Hydrogenases.
J. Am. Chem. Soc., 2021.
DOI: 10.1021/jacs.1c09554
HAL: hal-03445761
Matteo Sensi, Carole Baffert, Vincent Fourmond, Luca De Gioia, Luca Bertini, Christophe Léger.
Photochemistry and photoinhibition of the H-cluster of FeFe-hydrogenases.
Sustainable Energy Fuels, 2021, 5, 4248-4260.
DOI: 10.1039/D1SE00756D
HAL: hal-03358063
Vincent Fourmond, Nicolas Plumeré, Christophe Léger.
Reversible catalysis.
Nat Rev Chem, 2021, 5 (5).
DOI: 10.1038/s41570-021-00268-3
HAL: hal-03215154
Steffen Hardt, Stefanie Stapf, Dawit T. Filmon, James A. Birrell, Olaf Rüdiger, Vincent Fourmond, Christophe Léger, Nicolas Plumeré.
Reversible H2 oxidation and evolution by hydrogenase embedded in a redox polymer film.
Nature Catalysis, 2021, 4, 251-258.
DOI: 10.1038/s41929-021-00586-1
HAL: hal-03215114
(dataset available here)
Martin Winkler, Jifu Duan, Andreas Rutz, Christina Felbek, Lisa Scholtysek, Oliver Lampret, Jan Jaenecke, Ulf-Peter Apfel, Gianfranco Gilardi, Francesca Valetti, Vincent Fourmond, Eckhard Hofmann, Christophe Léger, Thomas Happe.
A safety cap protects hydrogenase from oxygen attack.
Nat Commun, 2021, 12 (1).
DOI: 10.1038/s41467-020-20861-2
HAL: hal-03129104
Aurore Jacq-Bailly, Martino Benvenuti, Natalie Payne, Arlette Kpebe, Christina Felbek, Vincent Fourmond, Christophe Léger, Myriam Brugna, Carole Baffert.
Electrochemical Characterization of a Complex FeFe Hydrogenase, the Electron-Bifurcating Hnd From Desulfovibrio fructosovorans.
Front. Chem., 2021, 8.
DOI: 10.3389/fchem.2020.573305
HAL: hal-03103487v1
Christina Felbek, Steffen Hardt, Cecilia Papini, Debajyoti Pramanik, Vincent Artero, Marc Fontecave, Vincent Fourmond, Nicolas Plumere, Christophe Léger.
Artificial maturation of [FeFe] hydrogenase in a redox polymer film.
Chem. Commun., 2021.
DOI: 10.1039/D0CC08168J
HAL: hal-03102775v1
2020
Baptiste Roumezi, Luisana Avilan, Véronique Risoul, Myriam Brugna, Sophie Rabouille, Amel Latifi.
Overproduction of the Flv3B flavodiiron, enhances the photobiological hydrogen production by the nitrogen-fixing cyanobacterium Nostoc PCC 7120.
Microb Cell Fact, 2020, 19 (1).
DOI: 10.1186/s12934-020-01320-5
HAL: hal-02539734
Ievgen Mazurenko, Vivek Pratap Hitaishi, Elisabeth Lojou.
Recent advances in surface chemistry of electrodes to promote direct enzymatic bioelectrocatalysis.
Current Opinion in Electrochemistry, 2020, 19, 113 – 121.
DOI: 10.1016/j.coelec.2019.11.004
HAL: hal-02395122v1
2019
Prakash Rewatkar, Vivek Pratap Hitaishi, Elisabeth Lojou, Sanket Goel.
Enzymatic fuel cells in a microfluidic environment: Status and opportunities. A mini review.
Electrochemistry Communications, 2019, 107, 106533.
DOI: 10.1016/j.elecom.2019.106533
HAL: hal-02375006v1
Huaiguang Li, Darren Buesen, Sébastien Dementin, Christophe Léger, Vincent Fourmond, Nicolas Plumeré.
Complete Protection of O2-Sensitive Catalysts in Thin Films.
J. Am. Chem. Soc., 2019, 141 (42), 16734 – 16742.
DOI: 10.1021/jacs.9b06790
HAL: hal-02369997v1
Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira.
A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase.
ACS Catal., 2019, 9 (9), 8509 – 8519.
DOI: 10.1021/acscatal.9b02347
HAL: hal-02278341v1
Xinxin Xiao, Hong-qi Xia, Ranran Wu, Lu Bai, Lu Yan, Edmond Magner, Serge Cosnier, Elisabeth Lojou, Zhiguang Zhu, Aihua Liu.
Tackling the Challenges of Enzymatic (Bio)Fuel Cells.
Chem. Rev., 2019, 119 (16), 9509 – 9558.
DOI: 10.1021/acs.chemrev.9b00115
HAL: hal-02167914v1
Vincent Fourmond, Eric S. Wiedner, Wendy J. Shaw, Christophe Léger.
Understanding and Design of Bidirectional and Reversible Catalysts of Multielectron, Multistep Reactions.
J. Am. Chem. Soc., 2019, 141 (28), 11269 – 11285.
DOI: 10.1021/jacs.9b04854
HAL: hal-02179792v1
Melisa del Barrio, Chloé Guendon, Arlette Kpebe, Carole Baffert, Vincent Fourmond, Myriam Brugna, Christophe Léger.
Valine-to-Cysteine Mutation Further Increases the Oxygen Tolerance of Escherichia coli NiFe Hydrogenase Hyd-1.
ACS Catal., 2019, 9 (5), 4084 – 4088.
DOI: 10.1021/acscatal.9b00543
HAL: hal-02104593v1
Carole Baffert, Arlette Kpebe, Luisana Avilan, Myriam Brugna.
Hydrogenases and H2 metabolism in sulfate-reducing bacteria of the Desulfovibrio genus..
Advances in Microbial Physiology., 2019, 74, 143 – 189.
DOI: 10.1016/bs.ampbs.2019.03.001
HAL: hal-02180360
Amel Latifi, Luisana Avilan, Myriam Brugna.
Clostridial whole cell and enzyme systems for hydrogen production: current state and perspectives.
Appl Microbiol Biotechnol, 2019, 196 (22), 567 – 575.
DOI: 10.1007/s00253-018-9514-3
HAL: hal-01928533v1
2018
Arlette Kpebe, Martino Benvenuti, Chloé Guendon, Amani Rebai, Victoria Fernandez, Sébastien Le Laz, Emilien Etienne, Bruno Guigliarelli, Gabriel García-Molina, Antonio L. de Lacey, Carole Baffert, Myriam Brugna.
A new mechanistic model for an O2-protected electron-bifurcating hydrogenase, Hnd from Desulfovibrio fructosovorans.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (12), 1302 – 1312.
DOI: 10.1016/j.bbabio.2018.09.364
HAL: hal-01928576v1
Vivek Hitaishi, Romain Clement, Nicolas Bourassin, Marc Baaden, Anne de Poulpiquet, Sophie Sacquin-Mora, Alexandre Ciaccafava, Elisabeth Lojou.
Controlling Redox Enzyme Orientation at Planar Electrodes.
Catalysts, 2018, 8 (5), 192.
DOI: 10.3390/catal8050192
HAL: hal-01793660v1
Luisana Avilan, Baptiste Roumezi, Véronique Risoul, Christophe Sébastien Bernard, Arlette Kpebe, Mayssène Belhadjhassine, Marc Rousset, Myriam Brugna, Amel Latifi.
Phototrophic hydrogen production from a clostridial [FeFe] hydrogenase expressed in the heterocysts of the cyanobacterium Nostoc PCC 7120.
Appl Microbiol Biotechnol, 2018, 66 (5), 1219 – 5783.
DOI: 10.1007/s00253-018-8989-2
HAL: hal-02353976v1
Melisa del Barrio, Matteo Sensi, Laura Fradale, Maurizio Bruschi, Claudio Greco, Luca de Gioia, Luca Bertini, Vincent Fourmond, Christophe Léger.
Interaction of the H-Cluster of FeFe Hydrogenase with Halides.
J. Am. Chem. Soc., 2018, 140 (16), 5485 – 5492.
DOI: 10.1021/jacs.8b01414
HAL: hal-01759718v1
Melisa del Barrio, Matteo Sensi, Christophe Orain, Carole Baffert, Sébastien Dementin, Vincent Fourmond, Christophe Léger.
Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels.
Acc. Chem. Res., 2018, 51 (3), 769 – 777.
DOI: 10.1021/acs.accounts.7b00622
HAL: hal-01745738v1
I. Mazurenko, R. Clément, D. Byrne-Kodjabachian, A. de Poulpiquet, S. Tsujimura, E. Lojou.
Pore size effect of MgO-templated carbon on enzymatic H2 oxidation by the hyperthermophilic hydrogenase from Aquifex aeolicus.
Journal of Electroanalytical Chemistry, 2018, 812, 221 – 226.
DOI: 10.1016/j.jelechem.2017.12.041
HAL: hal-01993863
Matteo Sensi, Christophe Orain, Carole Baffert, Vincent Fourmond et Christophe Léger.
La réaction des hydrogénases FeFe avec le dioxygène: étude expérimentale et théorique.
L’Actualité Chimique, 2018 (426).
(voir / see)
Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, David Guieysse, Laurence Girbal, Vincent Fourmond, Isabelle André, Bruno Guigliarelli, Christophe Léger, Philippe Soucaille, Isabelle Meynial-Salles.
Roles of the F-domain in [FeFe] hydrogenase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (2), 69 – 77.
DOI: 10.1016/j.bbabio.2017.08.010
HAL: cea-01950818
2017
Vincent Fourmond.
L’électroenzymologie, un outil pour étudier les enzymes redox.
L’Actualité Chimique, 2017 (424).
Matteo Sensi, Melisa del Barrio, Carole Baffert, Vincent Fourmond, Christophe Léger.
New perspectives in hydrogenase direct electrochemistry.
Current Opinion in Electrochemistry, 2017, 5 (1), 135 – 145.
DOI: 10.1016/j.coelec.2017.08.005
HAL: hal-01614142v1
Matteo Sensi, Carole Baffert, Laura Fradale, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Maurizio Bruschi, Vincent Fourmond, Christophe Léger, Luca Bertini.
Photoinhibition of FeFe Hydrogenase.
ACS Catal., 2017, 7 (10), 7378 – 7387.
DOI: 10.1021/acscatal.7b02252
HAL: hal-01613876v1
Christophe Léger.
Oxydation et production de dihydrogène : leçons de la nature.
Colloque “Biomimétisme et chimie durable”, Direction de la recherche et de l’innovation Service de la recherche, 2017.
(voir / see)
I. Mazurenko, X. Wang, A. de Poulpiquet, E. Lojou.
H 2 /O 2 enzymatic fuel cells: from proof-of-concept to powerful devices.
Sustainable Energy Fuels, 2017, 1 (7), 1475 – 1501.
DOI: 10.1039/C7SE00180K
HAL: hal-01552073v1
Ievgen Mazurenko, Karen Monsalve, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Frédéric Topin, Nicolas Mano, Elisabeth Lojou.
Impact of substrate diffusion and enzyme distribution in 3D-porous electrodes: a combined electrochemical and modelling study of a thermostable H 2 /O 2 enzymatic fuel cell.
Energy Environ. Sci., 2017, 10 (9), 1966 – 1982.
DOI: 10.1039/C7EE01830D
HAL: hal-01585310v1
Adam Kubas, Christophe Orain, David De Sancho, Laure Saujet, Matteo Sensi, Charles Gauquelin, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Carole Baffert, Vincent Fourmond, Robert B. Best, Jochen Blumberger, Christophe Léger.
Mechanism of O2 diffusion and reduction in FeFe hydrogenases.
Nature Chem, 2017, 11 (1), 205 – 95.
DOI: 10.1038/nchem.2592
HAL: hal-01406025v1
2016
Matteo Sensi, Carole Baffert, Claudio Greco, Giorgio Caserta, Charles Gauquelin, Laure Saujet, Marc Fontecave, Souvik Roy, Vincent Artero, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Vincent Fourmond, Christophe Léger, Luca B.
Reactivity of the Excited States of the H-Cluster of FeFe Hydrogenases.
J. Am. Chem. Soc., 2016, 138 (41), 13612 – 13618.
DOI: 10.1021/jacs.6b06603
HAL: hal-01889471v1
Karen Monsalve, Ievgen Mazurenko, Cristina Gutierrez-Sanchez, Marianne Ilbert, Pascale Infossi, Stefan Frielingsdorf, Marie Thérèse Giudici-Orticoni, Oliver Lenz, Elisabeth Lojou.
Impact of Carbon Nanotube Surface Chemistry on Hydrogen Oxidation by Membrane-Bound Oxygen-Tolerant Hydrogenases.
ChemElectroChem, 2016, 3 (12), 2179 – 2188.
DOI: 10.1002/celc.201600460
HAL: hal-01406080v1
Pierre Ceccaldi, Emilien Etienne, Sébastien Dementin, Bruno Guigliarelli, Christophe Léger, Bénédicte Burlat.
Mechanism of inhibition of NiFe hydrogenase by nitric oxide.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2016, 1857 (4), 454 – 461.
DOI: 10.1016/j.bbabio.2016.01.014
HAL: hal-01413192v1
2015
Karen Monsalve, Magali Roger, Cristina Gutierrez-Sanchez, Marianne Ilbert, Serge Nitsche, Deborah Byrne-Kodjabachian, Valérie Marchi, Elisabeth Lojou.
Hydrogen bioelectrooxidation on gold nanoparticle-based electrodes modified by Aquifex aeolicus hydrogenase: Application to hydrogen/oxygen enzymatic biofuel cells.
Bioelectrochemistry, 2015, 106, 47 – 55.
DOI: 10.1016/j.bioelechem.2015.04.010
HAL: hal-01150369v1
K. Monsalve, I. Mazurenko, N. Lalaoui, A. Le Goff, M. Holzinger, P. Infossi, S. Nitsche, J.Y. Lojou, M.T. Giudici-Orticoni, S. Cosnier, E. Lojou.
A H2/O2 enzymatic fuel cell as a sustainable power for a wireless device.
Electrochemistry Communications, 2015, 60, 216 – 220.
DOI: 10.1016/j.elecom.2015.09.014
HAL: hal-01432198v1
Christophe Orain, Laure Saujet, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Carole Baffert, Vincent Fourmond, Hervé Bottin, Christophe Léger.
Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 2 Demonstrate That the Reaction Is Partly Reversible.
J. Am. Chem. Soc., 2015, 137 (39), 12580 – 12587.
DOI: 10.1021/jacs.5b06934
HAL: hal-01211469v1
Vincent Fourmond, Stefanie Stapf, Huaiguang Li, Darren Buesen, James Birrell, Olaf Rüdiger, Wolfgang Lubitz, Wolfgang Schuhmann, Nicolas Plumeré, Christophe Léger.
Mechanism of Protection of Catalysts Supported in Redox Hydrogel Films.
J. Am. Chem. Soc., 2015, 137 (16), 5494 – 5505.
DOI: 10.1021/jacs.5b01194
HAL: hal-01428049v1
Abbas Abou-Hamdan, Pierre Ceccaldi, Hugo Lebrette, Oscar Gutiérrez-Sanz, Pierre Richaud, Laurent Cournac, Bruno Guigliarelli, Antonio L. De Lacey, Christophe Léger, Anne Volbeda, Bénédicte Burlat, Sébastien Dementin.
A Threonine Stabilizes the NiC and NiR Catalytic Intermediates of [NiFe]-hydrogenase.
J. Biol. Chem., 2015, 290 (13), 8550 – 8558.
DOI: 10.1074/jbc.M114.630491
HAL: hal-01149507v1
Noémie Lalaoui, Anne de Poulpiquet, Raoudha Haddad, Alan Le Goff, Michael Holzinger, Sébastien Gounel, Michel Mermoux, Pascale Infossi, Nicolas Mano, Elisabeth Lojou, Serge Cosnier.
A membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes.
Chem. Commun., 2015, 51 (35), 7447 – 7450.
DOI: 10.1039/C5CC02166A
HAL: hal-01145429v1
Vincent Fourmond, Carole Baffert, Sébastien Dementin, Christophe Léger.
L’électrochimie, un outil pour étudier les mécanismes enzymatiques.
L’Actualité Chimique, 2015 (392).
Pierre Ceccaldi, Marta C. Marques, Vincent Fourmond, Inês Cardoso Pereira, Christophe Léger.
Oxidative inactivation of NiFeSe hydrogenase.
Chem. Commun., 2015, 562 (75), 43 – 14226.
DOI: 10.1039/C5CC05930E
HAL: hal-01211493v1
2014
Francesco Oteri, Marc Baaden, Elisabeth Lojou, Sophie Sacquin-Mora.
Multiscale Simulations Give Insight into the Hydrogen In and Out Pathways of [NiFe]-Hydrogenases from Aquifex aeolicus and Desulfovibrio fructosovorans.
J. Phys. Chem. B, 2014, 118 (48), 13800 – 13811.
DOI: 10.1021/jp5089965
HAL: hal-01588442v1
Anne de Poulpiquet, David Ranava, Karen Monsalve, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective.
CHEMELECTROCHEM, 2014, 1 (11), 1724 – 1750.
DOI: 10.1002/celc.201402249
A. de Poulpiquet, A. Ciaccafava, R. Gadiou, S. Gounel, M.T. Giudici-Orticoni, N. Mano, E. Lojou.
Design of a H2/O2 biofuel cell based on thermostable enzymes.
Electrochemistry Communications, 2014, 42, 72 – 74.
DOI: 10.1016/j.elecom.2014.02.012
HAL: hal-00961067v1
Vincent Fourmond, Claudio Greco, Kateryna Sybirna, Carole Baffert, Po-Hung Wang, Pierre Ezanno, Marco Montefiori, Maurizio Bruschi, Isabelle Meynial-Salles, Philippe Soucaille, Jochen Blumberger, Hervé Bottin, Luca De Gioia, Christophe Léger.
The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster.
Nature Chem, 2014, 130 (4), 4533 – 342.
DOI: 10.1038/nchem.1892
HAL: hal-01481520v1
Anne de Poulpiquet, Helena Marques-Knopf, Véronique Wernert, Marie Thérèse Giudici-Orticoni, Roger Gadiou, Elisabeth Lojou.
Carbon nanofiber mesoporous films: efficient platforms for bio-hydrogen oxidation in biofuel cells.
Phys. Chem. Chem. Phys., 2014, 16 (4), 1366 – 1378.
DOI: 10.1039/C3CP54631D
HAL: hal-01493456v1
Claudio Greco, Vincent Fourmond, Carole Baffert, Po-hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger.
Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes.
Energy Environ. Sci., 2014, 108 (11), 2066 – 3573.
DOI: 10.1039/C4EE01848F
HAL: hal-01211585v1
Viviane Hajj, Carole Baffert, Kateryna Sybirna, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Vincent Fourmond, Christophe Léger.
FeFe hydrogenase reductive inactivation and implication for catalysis.
Energy Environ. Sci., 2014, 6 (2), 2460 – 719.
DOI: 10.1039/C3EE42075B
HAL: hal-01481475v1
C. Hamon, A. Ciaccafava, P. Infossi, R. Puppo, P. Even-Hernandez, E. Lojou, V. Marchi.
Synthesis and enzymatic photo-activity of an O 2 tolerant hydrogenase–CdSe@CdS quantum rod bioconjugate.
Chem. Commun., 2014, 50 (8), 4989 – 4992.
DOI: 10.1039/C3CC49368G
HAL: hal-01114425v1
Francesco Oteri, Alexandre Ciaccafava, Anne de Poulpiquet, Marc Baaden, Elisabeth Lojou, Sophie Sacquin-Mora.
The weak, fluctuating, dipole moment of membrane-bound hydrogenase from Aquifex aeolicus accounts for its adaptability to charged electrodes.
Phys. Chem. Chem. Phys., 2014, 21 (23), 11318 – 11322.
DOI: 10.1039/C4CP00510D
HAL: hal-01493492v1
Sébastien Le Laz, Arlette Kpebe, Jean Lorquin, Myriam Brugna, Marc Rousset.
H2-dependent azoreduction by Shewanella oneidensis MR-1: involvement of secreted flavins and both [Ni–Fe] and [Fe–Fe] hydrogenases.
Appl Microbiol Biotechnol, 2014, 73 (6), 7759 – 2707.
DOI: 10.1007/s00253-013-5208-z
HAL: hal-01494493v1
2013
Katarzyna Szot, Anne de Poulpiquet, Alexandre Ciaccafava, Helena Marques, Martin Jönsson-Niedziolka, Joanna Niedziolka-Jönsson, Frank Marken, Elisabeth Lojou, Marcin Opallo.
Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation.
Electrochimica Acta, 2013, 111, 434 – 440.
DOI: 10.1016/j.electacta.2013.08.001
HAL: hal-03079276
Toshiko Miyake, Maurizio Bruschi, Ugo Cosentino, Carole Baffert, Vincent Fourmond, Christophe Léger, Giorgio Moro, Luca De Gioia, Claudio Greco.
Does the environment around the H-cluster allow coordination of the pendant amine to the catalytic iron center in [FeFe] hydrogenases? Answers from theory.
J Biol Inorg Chem, 2013, 13 (6), 3580 – 700.
DOI: 10.1007/s00775-013-1014-4
HAL: hal-03023214
Kateryna Sybirna, Pierre Ezanno, Carole Baffert, Christophe Léger, Hervé Bottin.
Arginine171 of Chlamydomonas reinhardtii [Fe–Fe] hydrogenase HydA1 plays a crucial role in electron transfer to its catalytic center.
International Journal of Hydrogen Energy, 2013, 38 (7), 2998 – 3002.
DOI: 10.1016/j.ijhydene.2012.12.078
Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Thomas Lautier, Abbas Abou Hamdan, Sébastien Dementin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Christophe Léger.
Steady-State Catalytic Wave-Shapes for 2-Electron Reversible Electrocatalysts and Enzymes.
J. Am. Chem. Soc., 2013, 135 (10), 3926 – 3938.
DOI: 10.1021/ja311607s
HAL: hal-01268145v1
Anne De Poulpiquet, Alexandre Ciaccafava, Katarzyna Szot, Baptiste Pillain, Pascale Infossi, Marianne Guiral, Marcin Opallo, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Exploring Properties of a Hyperthermophilic Membrane-Bound Hydrogenase at Carbon Nanotube Modified Electrodes for a Powerful H 2 /O 2 Biofuel Cell.
Electroanalysis, 2013, 284 (3), 465 – 695.
DOI: 10.1002/elan.201200405
HAL: hal-03079262
Alexandre Ciaccafava, Cyrille Hamon, Pascale Infossi, Valérie Marchi, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Light-induced reactivation of O2-tolerant membrane-bound [Ni–Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions.
Phys. Chem. Chem. Phys., 2013, 15 (39), 16463 – 16467.
DOI: 10.1039/c3cp52596a
HAL: hal-00860457v1
Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Sébastien Dementin, Abbas Abou-Hamdan, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
The mechanism of inhibition by H2 of H2-evolution by hydrogenases.
Chem. Commun., 2013, 109 (61), 19916 –.
DOI: 10.1039/C3CC43297A
HAL: hal-01268212v1
Abbas Abou Hamdan, Bénédicte Burlat, Oscar Gutiérrez-Sanz, Pierre-Pol Liebgott, Carole Baffert, Antonio L De Lacey, Marc Rousset, Bruno Guigliarelli, Christophe Léger, Sébastien Dementin.
O2-independent formation of the inactive states of NiFe hydrogenase.
Nat Chem Biol, 2013, 9, 15 – 17.
DOI: 10.1038/NCHEMBIO.1110
HAL: hal-01556905v1
2012
A. Abou Hamdan, P.-P. Liebgott, V. Fourmond, O. Gutierrez-Sanz, A. L. De Lacey, P. Infossi, M. Rousset, S. Dementin, C. Leger.
Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants.
Proceedings of the National Academy of Sciences, 2012, 76 (1-2), 141 – 19921.
DOI: 10.1073/pnas.1212258109
HAL: hal-01825482v1
A. Ciaccafava, A. De Poulpiquet, P. Infossi, S. Robert, R. Gadiou, M.T. Giudici-Orticoni, S. Lecomte, E. Lojou.
A friendly detergent for H2 oxidation by Aquifex aeolicus membrane-bound hydrogenase immobilized on graphite and Self-Assembled-Monolayer-modified gold electrodes.
Electrochimica Acta, 2012, 82, 115 – 125.
DOI: 10.1016/j.electacta.2012.03.034
HAL: hal-03079175
A. Ciaccafava, A. De Poulpiquet, V. Techer, M.T. Giudici-Orticoni, S. Tingry, C. Innocent, E. Lojou.
An innovative powerful and mediatorless H2/O2 biofuel cell based on an outstanding bioanode.
Electrochemistry Communications, 2012, 23, 25 – 28.
DOI: 10.1016/j.elecom.2012.06.035
HAL: hal-01690641v1
Carole Baffert, Kateryna Sybirna, Pierre Ezanno, Thomas Lautier, Viviane Hajj, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
Covalent Attachment of FeFe Hydrogenases to Carbon Electrodes for Direct Electron Transfer.
Anal. Chem., 2012, 84 (18), 7999 – 8005.
DOI: 10.1021/ac301812s
HAL: hal-01268277v1
Abbas Abou Hamdan, Sébastien Dementin, Pierre-Pol Liebgott, Oscar Gutierrez-Sanz, Pierre Richaud, Antonio L. De Lacey, Marc Rousset, Patrick Bertrand, Laurent Cournac, Christophe Léger.
Understanding and Tuning the Catalytic Bias of Hydrogenase.
J. Am. Chem. Soc., 2012, 134 (20), 8368 – 8371.
DOI: 10.1021/ja301802r
HAL: hal-00748324v1
Alexandre Ciaccafava, Pascale Infossi, Marianne Ilbert, Marianne Guiral, Sophie Lecomte, Marie Thérèse Giudici-Orticoni, Elisabeth Lojou.
Electrochemistry, AFM, and PM-IRRA Spectroscopy of Immobilized Hydrogenase: Role of a Hydrophobic Helix in Enzyme Orientation for Efficient H2 Oxidation.
Angew. Chem. Int. Ed., 2012, 51 (4), 953 – 956.
DOI: 10.1002/anie.201107053
2011
Elisabeth Lojou.
Hydrogenases as catalysts for fuel cells: Strategies for efficient immobilization at electrode interfaces.
Electrochimica Acta, 2011, 56 (28), 10385 – 10397.
DOI: 10.1016/j.electacta.2011.03.002
HAL: hal-00677206
Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 – 10221.
DOI: 10.1021/ja202615a
HAL: hal-00677402v1
A. Ciaccafava, M. Alberola, S. Hameury, P. Infossi, M.T. Giudici-Orticoni, E. Lojou.
Hydrogen bioelectrooxidation in ionic liquids: From cytochrome c3 redox behavior to hydrogenase activity.
Electrochimica Acta, 2011, 56 (9), 3359 – 3368.
DOI: 10.1016/j.electacta.2010.12.104
HAL: hal-00677396
Carole Baffert, Luca Bertini, Thomas Lautier, Claudio Greco, Kateryna Sybirna, Pierre Ezanno, Emilien Etienne, Philippe Soucaille, Patrick Bertrand, Hervé Bottin, Isabelle Meynial-Salles, Luca De Gioia, Christophe Léger.
CO Disrupts the Reduced H-Cluster of FeFe Hydrogenase. A Combined DFT and Protein Film Voltammetry Study.
J. Am. Chem. Soc., 2011, 133 (7), 2096 – 2099.
DOI: 10.1021/ja110627b
HAL: hal-02652182v1
Pierre-Pol Liebgott, Antonio L. de Lacey, Bénédicte Burlat, Laurent Cournac, Pierre Richaud, Myriam Brugna, Victor M. Fernandez, Bruno Guigliarelli, Marc Rousset, Christophe Léger, Sébastien Dementin.
Original Design of an Oxygen-Tolerant [NiFe] Hydrogenase: Major Effect of a Valine-to-Cysteine Mutation near the Active Site.
J. Am. Chem. Soc., 2011, 133 (4), 986 – 997.
DOI: 10.1021/ja108787s
HAL: hal-01977599v1
Vincent Fourmond, Sigolène Canaguier, Benjamin Golly, Martin J. Field, Marc Fontecave, Vincent Artero.
A nickel–manganese catalyst as a biomimic of the active site of NiFe hydrogenases: a combined electrocatalytical and DFT mechanistic study.
Energy Environ. Sci., 2011, 76 (7), 141 –.
DOI: 10.1039/C0EE00736F
HAL: hal-01139836v1
Thomas Lautier, Pierre Ezanno, Carole Baffert, Vincent Fourmond, Laurent Cournac, Juan C. Fontecilla-Camps, Philippe Soucaille, Patrick Bertrand, Isabelle Meynial-Salles, Christophe Léger.
The quest for a functional substrate access tunnel in FeFe hydrogenase.
Faraday Discuss., 2011, 304, 55 – 407.
DOI: 10.1039/c004099c
HAL: hal-02646834
Pierre-Pol Liebgott, Sébastien Dementin, Christophe Léger, Marc Rousset.
Towards engineering O2-tolerance in [Ni–Fe] hydrogenases.
Energy Environ. Sci., 2011, 128 (1), 5209 – 41.
DOI: 10.1039/c0ee00093k
HAL: hal-01977604
2010
Alexandre Ciaccafava, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Stabilization Role of a Phenothiazine Derivative on the Electrocatalytic Oxidation of Hydrogen via Aquifex aeolicus Hydrogenase at Graphite Membrane Electrodes.
Langmuir, 2010, 26 (23), 18534 – 18541.
DOI: 10.1021/la103714n
HAL: hal-00677650
Vincent Fourmond, Pierre-André Jacques, Marc Fontecave, Vincent Artero.
H2 Evolution and Molecular Electrocatalysts: Determination of Overpotentials and Effect of Homoconjugation.
Inorg. Chem., 2010, 49 (22), 10338 – 10347.
DOI: 10.1021/ic101187v
HAL: hal-01069160v1
Pascale Infossi, Elisabeth Lojou, Jean-Paul Chauvin, Gaetan Herbette, Myriam Brugna, Marie-Thérèse Giudici-Orticoni.
Aquifex aeolicus membrane hydrogenase for hydrogen biooxidation: Role of lipids and physiological partners in enzyme stability and activity.
International Journal of Hydrogen Energy, 2010, 35 (19), 10778 – 10789.
DOI: 10.1016/j.ijhydene.2010.02.054
HAL: hal-00686860v1
Fanny Leroux, Pierre-Pol Liebgott, Laurent Cournac, Pierre Richaud, Arlette Kpebe, Bénedicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Christophe Léger, Marc Rousset.
Is engineering O2-tolerant hydrogenases just a matter of reproducing the active sites of the naturally occurring O2-resistant enzymes?.
International Journal of Hydrogen Energy, 2010, 35 (19), 10770 – 10777.
DOI: 10.1016/j.ijhydene.2010.02.071
HAL: hal-01977635v1
Maria-Eirini Pandelia, Vincent Fourmond, Pascale Tron-Infossi, Elisabeth Lojou, Patrick Bertrand, Christophe Léger, Marie-Thérèse Giudici-Orticoni, Wolfgang Lubitz.
Membrane-Bound Hydrogenase I from the Hyperthermophilic Bacterium Aquifex aeolicus: Enzyme Activation, Redox Intermediates and Oxygen Tolerance.
J. Am. Chem. Soc., 2010, 132 (20), 6991 – 7004.
DOI: 10.1021/ja910838d
HAL: hal-00686880v1
Vincent Fourmond, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Patrick Bertrand, Christophe Léger.
“Two-Step” Chronoamperometric Method for Studying the Anaerobic Inactivation of an Oxygen Tolerant NiFe Hydrogenase.
J. Am. Chem. Soc., 2010, 132 (13), 4848 – 4857.
DOI: 10.1021/ja910685j
HAL: hal-00677470
Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 6, 63 – 70.
DOI: 10.1038/nchembio.276
HAL: hal-01977629
2009
Sébastien Dementin, Fanny Leroux, Laurent Cournac, Antonio L. de Lacey, Anne Volbeda, Christophe Léger, Bénédicte Burlat, Nicolas Martinez, Stéphanie Champ, Lydie Martin, Oliver Sanganas, Michael Haumann, Víctor M. Fernández, Bruno Guigliarelli, Ju.
Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant.
J. Am. Chem. Soc., 2009, 131 (29), 10156 – 10164.
DOI: 10.1021/ja9018258
Xiaojun Luo, Myriam Brugna, Pascale Tron-Infossi, Marie Thérèse Giudici-Orticoni, Élisabeth Lojou.
Immobilization of the hyperthermophilic hydrogenase from Aquifex aeolicus bacterium onto gold and carbon nanotube electrodes for efficient H2 oxidation.
J Biol Inorg Chem, 2009, 14 (8), 1275 – 1288.
DOI: 10.1007/s00775-009-0572-y
2008
F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset, C. Leger.
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences, 2008, 105 (32), 11188 – 11193.
DOI: 10.1073/pnas.0803689105
HAL: hal-00336010v1
É. Lojou, X. Luo, M. Brugna, N. Candoni, S. Dementin, M. T. Giudici-Orticoni.
Biocatalysts for fuel cells: efficient hydrogenase orientation for H2 oxidation at electrodes modified with carbon nanotubes.
J Biol Inorg Chem, 2008, 13 (7), 1157 – 1167.
DOI: 10.1007/s00775-008-0401-8
HAL: hal-00335503
Carole Baffert, Marie Demuez, Laurent Cournac, Bénédicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Laurence Girbal, Christophe Léger.
Hydrogen-Activating Enzymes: Activity Does Not Correlate with Oxygen Sensitivity.
Angew. Chem. Int. Ed., 2008, 47 (11), 2052 – 2054.
DOI: 10.1002/anie.200704313
HAL: hal-00336018
2006
M GUIRAL, P TRON, V BELLE, C AUBERT, C LEGER, B GUIGLIARELLI, M GIUDICIORTICONI.
Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties.
International Journal of Hydrogen Energy, 2006, 31 (11), 1424 – 1431.
DOI: 10.1016/j.ijhydene.2006.06.007
HAL: hal-00335435
Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 – 5218.
DOI: 10.1021/ja060233b
HAL: hal-00335157v1
2005
Élisabeth Lojou, Marie-Thérèse Giudici-Orticoni, Pierre Bianco.
Hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: electrocatalysis of the hydrogen production/consumption reactions at carbon electrodes.
Journal of Electroanalytical Chemistry, 2005, 577 (1), 79 – 86.
DOI: 10.1016/j.jelechem.2004.11.016
2004
Christophe Léger, Sébastien Dementin, Patrick Bertrand, Marc Rousset, Bruno Guigliarelli.
Inhibition and Aerobic Inactivation Kinetics of Desulfovibrio fructosovorans NiFe Hydrogenase Studied by Protein Film Voltammetry.
J. Am. Chem. Soc., 2004, 126 (38), 12162 – 12172.
DOI: 10.1021/ja046548d
HAL: hal-00336069
É. Lojou, P. Bianco.
Electrocatalytic Reactions at Hydrogenase-Modified Electrodes and Their Applications to Biosensors: From the Isolated Enzymes to the Whole Cells.
Electroanalysis, 2004, 16 (1314), 1093 – 1100.
DOI: 10.1002/elan.200403002
2003
Marianne Brugna-Guiral, Pascale Tron, Wolfgang Nitschke, Karl-Otto Stetter, Benedicte Burlat, Bruno Guigliarelli, Mireille Bruschi, Marie Thérèse Giudici-Orticoni.
[NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics.
Extremophiles, 2003, 139 (2), 139 – 157.
DOI: 10.1007/s00792-002-0306-3
HAL: hal-00343693v1
2002
E. Lojou, M. C. Durand, A. Dolla, P. Bianco.
Hydrogenase Activity Control at Desulfovibrio vulgaris Cell-Coated Carbon Electrodes: Biochemical and Chemical Factors Influencing the Mediated Bioelectrocatalysis.
Electroanalysis, 2002, 14 (13), 913 –.
DOI: 10.1002/1521-4109(200207)14:133.0.CO;2-N