Hydrogène et métabolismes associés

Hydrogen and associated metabolisms

Publications

2020

Baptiste Roumezi, Luisana Avilan, Véronique Risoul, Myriam Brugna, Sophie Rabouille, Amel Latifi.
Overproduction of the Flv3B flavodiiron, enhances the photobiological hydrogen production by the nitrogen-fixing cyanobacterium Nostoc PCC 7120.
Microb Cell Fact, 2020, 19 (1). DOI: 10.1186/s12934-020-01320-5

Ievgen Mazurenko, Vivek Pratap Hitaishi, Elisabeth Lojou.
Recent advances in surface chemistry of electrodes to promote direct enzymatic bioelectrocatalysis.
Current Opinion in Electrochemistry, 2020, 19, 113 – 121. DOI: 10.1016/j.coelec.2019.11.004 HAL: hal-02395122v1



2019

Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira.
A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase.
ACS Catal., 2019, 9 (9), 8509 – 8519. DOI: 10.1021/acscatal.9b02347 HAL: hal-02278341v1

Amel Latifi, Luisana Avilan, Myriam Brugna.
Clostridial whole cell and enzyme systems for hydrogen production: current state and perspectives.
Appl Microbiol Biotechnol, 2019, 196 (22), 567 – 575. DOI: 10.1007/s00253-018-9514-3 HAL: hal-01928533v1

Huaiguang Li, Darren Buesen, Sébastien Dementin, Christophe Léger, Vincent Fourmond, Nicolas Plumeré.
Complete Protection of O2-Sensitive Catalysts in Thin Films.
J. Am. Chem. Soc., 2019, 141 (42), 16734 – 16742. DOI: 10.1021/jacs.9b06790 HAL: hal-02369997v1

Prakash Rewatkar, Vivek Pratap Hitaishi, Elisabeth Lojou, Sanket Goel.
Enzymatic fuel cells in a microfluidic environment: Status and opportunities. A mini review.
Electrochemistry Communications, 2019, 107, 106533 –. DOI: 10.1016/j.elecom.2019.106533 HAL: hal-02375006v1

Carole Baffert, Arlette Kpebe, Luisana Avilan, Myriam Brugna.
Hydrogenases and H2 metabolism in sulfate-reducing bacteria of the Desulfovibrio genus..
Advances in Microbial Physiology., 2019, 74, 143 – 189. DOI: 10.1016/bs.ampbs.2019.03.001

Xinxin Xiao, Hong-qi Xia, Ranran Wu, Lu Bai, Lu Yan, Edmond Magner, Serge Cosnier, Elisabeth Lojou, Zhiguang Zhu, Aihua Liu.
Tackling the Challenges of Enzymatic (Bio)Fuel Cells.
Chem. Rev., 2019, 119 (16), 9509 – 9558. DOI: 10.1021/acs.chemrev.9b00115 HAL: hal-02167914v1

Vincent Fourmond, Eric S. Wiedner, Wendy J. Shaw, Christophe Léger.
Understanding and Design of Bidirectional and Reversible Catalysts of Multielectron, Multistep Reactions.
J. Am. Chem. Soc., 2019, 141 (28), 11269 – 11285. DOI: 10.1021/jacs.9b04854 HAL: hal-02179792v1

Melisa del Barrio, Chloé Guendon, Arlette Kpebe, Carole Baffert, Vincent Fourmond, Myriam Brugna, Christophe Léger.
Valine-to-Cysteine Mutation Further Increases the Oxygen Tolerance of Escherichia coli NiFe Hydrogenase Hyd-1.
ACS Catal., 2019, 9 (5), 4084 – 4088. DOI: 10.1021/acscatal.9b00543 HAL: hal-02104593v1



2018

Arlette Kpebe, Martino Benvenuti, Chloé Guendon, Amani Rebai, Victoria Fernandez, Sébastien Le Laz, Emilien Etienne, Bruno Guigliarelli, Gabriel García-Molina, Antonio L. de Lacey, Carole Baffert, Myriam Brugna.
A new mechanistic model for an O2-protected electron-bifurcating hydrogenase, Hnd from Desulfovibrio fructosovorans.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (12), 1302 – 1312. DOI: 10.1016/j.bbabio.2018.09.364 HAL: hal-01928576v1

Vivek Hitaishi, Romain Clement, Nicolas Bourassin, Marc Baaden, Anne de Poulpiquet, Sophie Sacquin-Mora, Alexandre Ciaccafava, Elisabeth Lojou.
Controlling Redox Enzyme Orientation at Planar Electrodes.
Catalysts, 2018, 8 (5), 192. DOI: 10.3390/catal8050192 HAL: hal-01793660v1

Melisa del Barrio, Matteo Sensi, Christophe Orain, Carole Baffert, Sébastien Dementin, Vincent Fourmond, Christophe Léger.
Electrochemical Investigations of Hydrogenases and Other Enzymes That Produce and Use Solar Fuels.
Acc. Chem. Res., 2018, 51 (3), 769 – 777. DOI: 10.1021/acs.accounts.7b00622 HAL: hal-01745738v1

Melisa del Barrio, Matteo Sensi, Laura Fradale, Maurizio Bruschi, Claudio Greco, Luca de Gioia, Luca Bertini, Vincent Fourmond, Christophe Léger.
Interaction of the H-Cluster of FeFe Hydrogenase with Halides.
J. Am. Chem. Soc., 2018, 140 (16), 5485 – 5492. DOI: 10.1021/jacs.8b01414 HAL: hal-01759718v1

Luisana Avilan, Baptiste Roumezi, Véronique Risoul, Christophe Sébastien Bernard, Arlette Kpebe, Mayssène Belhadjhassine, Marc Rousset, Myriam Brugna, Amel Latifi.
Phototrophic hydrogen production from a clostridial [FeFe] hydrogenase expressed in the heterocysts of the cyanobacterium Nostoc PCC 7120.
Appl Microbiol Biotechnol, 2018, 66 (5), 1219 – 5783. DOI: 10.1007/s00253-018-8989-2 HAL: hal-02353976v1

I. Mazurenko, R. Clément, D. Byrne-Kodjabachian, A. de Poulpiquet, S. Tsujimura, E. Lojou.
Pore size effect of MgO-templated carbon on enzymatic H2 oxidation by the hyperthermophilic hydrogenase from Aquifex aeolicus.
Journal of Electroanalytical Chemistry, 2018, 812, 221 – 226. DOI: 10.1016/j.jelechem.2017.12.041 HAL: hal-01993863

Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, David Guieysse, Laurence Girbal, Vincent Fourmond, Isabelle André, Bruno Guigliarelli, Christophe Léger, Philippe Soucaille, Isabelle Meynial-Salles.
Roles of the F-domain in [FeFe] hydrogenase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (2), 69 – 77. DOI: 10.1016/j.bbabio.2017.08.010 HAL: cea-01950818



2017

I. Mazurenko, X. Wang, A. de Poulpiquet, E. Lojou.
H 2 /O 2 enzymatic fuel cells: from proof-of-concept to powerful devices.
Sustainable Energy Fuels, 2017, 1 (7), 1475 – 1501. DOI: 10.1039/C7SE00180K HAL: hal-01552073v1

Ievgen Mazurenko, Karen Monsalve, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Frédéric Topin, Nicolas Mano, Elisabeth Lojou.
Impact of substrate diffusion and enzyme distribution in 3D-porous electrodes: a combined electrochemical and modelling study of a thermostable H 2 /O 2 enzymatic fuel cell.
Energy Environ. Sci., 2017, 10 (9), 1966 – 1982. DOI: 10.1039/C7EE01830D HAL: hal-01585310v1

Adam Kubas, Christophe Orain, David De Sancho, Laure Saujet, Matteo Sensi, Charles Gauquelin, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Carole Baffert, Vincent Fourmond, Robert B. Best, Jochen Blumberger, Christophe Léger.
Mechanism of O2 diffusion and reduction in FeFe hydrogenases.
Nature Chem, 2017, 11 (1), 205 – 95. DOI: 10.1038/nchem.2592 HAL: hal-01406025v1

Matteo Sensi, Melisa del Barrio, Carole Baffert, Vincent Fourmond, Christophe Léger.
New perspectives in hydrogenase direct electrochemistry.
Current Opinion in Electrochemistry, 2017, 5 (1), 135 – 145. DOI: 10.1016/j.coelec.2017.08.005 HAL: hal-01614142v1

Matteo Sensi, Carole Baffert, Laura Fradale, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Maurizio Bruschi, Vincent Fourmond, Christophe Léger, Luca Bertini.
Photoinhibition of FeFe Hydrogenase.
ACS Catal., 2017, 7 (10), 7378 – 7387. DOI: 10.1021/acscatal.7b02252 HAL: hal-01613876v1



2016

Karen Monsalve, Ievgen Mazurenko, Cristina Gutierrez-Sanchez, Marianne Ilbert, Pascale Infossi, Stefan Frielingsdorf, Marie Thérèse Giudici-Orticoni, Oliver Lenz, Elisabeth Lojou.
Impact of Carbon Nanotube Surface Chemistry on Hydrogen Oxidation by Membrane-Bound Oxygen-Tolerant Hydrogenases.
ChemElectroChem, 2016, 3 (12), 2179 – 2188. DOI: 10.1002/celc.201600460 HAL: hal-01406080v1

Pierre Ceccaldi, Emilien Etienne, Sébastien Dementin, Bruno Guigliarelli, Christophe Léger, Bénédicte Burlat.
Mechanism of inhibition of NiFe hydrogenase by nitric oxide.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2016, 1857 (4), 454 – 461. DOI: 10.1016/j.bbabio.2016.01.014 HAL: hal-01413192v1

Matteo Sensi, Carole Baffert, Claudio Greco, Giorgio Caserta, Charles Gauquelin, Laure Saujet, Marc Fontecave, Souvik Roy, Vincent Artero, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Luca de Gioia, Vincent Fourmond, Christophe Léger, Luca B.
Reactivity of the Excited States of the H-Cluster of FeFe Hydrogenases.
J. Am. Chem. Soc., 2016, 138 (41), 13612 – 13618. DOI: 10.1021/jacs.6b06603 HAL: hal-01889471v1



2015

K. Monsalve, I. Mazurenko, N. Lalaoui, A. Le Goff, M. Holzinger, P. Infossi, S. Nitsche, J.Y. Lojou, M.T. Giudici-Orticoni, S. Cosnier, E. Lojou.
A H2/O2 enzymatic fuel cell as a sustainable power for a wireless device.
Electrochemistry Communications, 2015, 60, 216 – 220. DOI: 10.1016/j.elecom.2015.09.014 HAL: hal-01432198v1

Noémie Lalaoui, Anne de Poulpiquet, Raoudha Haddad, Alan Le Goff, Michael Holzinger, Sébastien Gounel, Michel Mermoux, Pascale Infossi, Nicolas Mano, Elisabeth Lojou, Serge Cosnier.
A membraneless air-breathing hydrogen biofuel cell based on direct wiring of thermostable enzymes on carbon nanotube electrodes.
Chem. Commun., 2015, 51 (35), 7447 – 7450. DOI: 10.1039/C5CC02166A HAL: hal-01145429v1

Abbas Abou-Hamdan, Pierre Ceccaldi, Hugo Lebrette, Oscar Gutiérrez-Sanz, Pierre Richaud, Laurent Cournac, Bruno Guigliarelli, Antonio L. De Lacey, Christophe Léger, Anne Volbeda, Bénédicte Burlat, Sébastien Dementin.
A Threonine Stabilizes the NiC and NiR Catalytic Intermediates of [NiFe]-hydrogenase.
J. Biol. Chem., 2015, 1837 (13), 131 – 8558. DOI: 10.1074/jbc.M114.630491 HAL: hal-01149507v1

Christophe Orain, Laure Saujet, Charles Gauquelin, Philippe Soucaille, Isabelle Meynial-Salles, Carole Baffert, Vincent Fourmond, Hervé Bottin, Christophe Léger.
Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 2 Demonstrate That the Reaction Is Partly Reversible.
J. Am. Chem. Soc., 2015, 137 (39), 12580 – 12587. DOI: 10.1021/jacs.5b06934 HAL: hal-01211469v1

Karen Monsalve, Magali Roger, Cristina Gutierrez-Sanchez, Marianne Ilbert, Serge Nitsche, Deborah Byrne-Kodjabachian, Valérie Marchi, Elisabeth Lojou.
Hydrogen bioelectrooxidation on gold nanoparticle-based electrodes modified by Aquifex aeolicus hydrogenase: Application to hydrogen/oxygen enzymatic biofuel cells.
Bioelectrochemistry, 2015, 106, 47 – 55. DOI: 10.1016/j.bioelechem.2015.04.010 HAL: hal-01150369v1

Vincent Fourmond, Stefanie Stapf, Huaiguang Li, Darren Buesen, James Birrell, Olaf Rüdiger, Wolfgang Lubitz, Wolfgang Schuhmann, Nicolas Plumeré, Christophe Léger.
Mechanism of Protection of Catalysts Supported in Redox Hydrogel Films.
J. Am. Chem. Soc., 2015, 137 (16), 5494 – 5505. DOI: 10.1021/jacs.5b01194 HAL: hal-01428049v1

Pierre Ceccaldi, Marta C. Marques, Vincent Fourmond, Inês Cardoso Pereira, Christophe Léger.
Oxidative inactivation of NiFeSe hydrogenase.
Chem. Commun., 2015, 562 (75), 43 – 14226. DOI: 10.1039/C5CC05930E HAL: hal-01211493v1



2014

Anne de Poulpiquet, David Ranava, Karen Monsalve, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Biohydrogen for a New Generation of H2/O2 Biofuel Cells: A Sustainable Energy Perspective.
CHEMELECTROCHEM, 2014, 1 (11), 1724 – 1750. DOI: 10.1002/celc.201402249

Anne de Poulpiquet, Helena Marques-Knopf, Véronique Wernert, Marie Thérèse Giudici-Orticoni, Roger Gadiou, Elisabeth Lojou.
Carbon nanofiber mesoporous films: efficient platforms for bio-hydrogen oxidation in biofuel cells.
Phys. Chem. Chem. Phys., 2014, 16 (4), 1366 – 1378. DOI: 10.1039/C3CP54631D HAL: hal-01493456v1

Claudio Greco, Vincent Fourmond, Carole Baffert, Po-hung Wang, Sébastien Dementin, Patrick Bertrand, Maurizio Bruschi, Jochen Blumberger, Luca de Gioia, Christophe Léger.
Combining experimental and theoretical methods to learn about the reactivity of gas-processing metalloenzymes.
Energy Environ. Sci., 2014, 108 (11), 2066 – 3573. DOI: 10.1039/C4EE01848F HAL: hal-01211585v1

A. de Poulpiquet, A. Ciaccafava, R. Gadiou, S. Gounel, M.T. Giudici-Orticoni, N. Mano, E. Lojou.
Design of a H2/O2 biofuel cell based on thermostable enzymes.
Electrochemistry Communications, 2014, 42, 72 – 74. DOI: 10.1016/j.elecom.2014.02.012 HAL: hal-00961067v1

Viviane Hajj, Carole Baffert, Kateryna Sybirna, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Vincent Fourmond, Christophe Léger.
FeFe hydrogenase reductive inactivation and implication for catalysis.
Energy Environ. Sci., 2014, 6 (2), 2460 – 719. DOI: 10.1039/C3EE42075B HAL: hal-01481475v1

Sébastien Le Laz, Arlette Kpebe, Jean Lorquin, Myriam Brugna, Marc Rousset.
H2-dependent azoreduction by Shewanella oneidensis MR-1: involvement of secreted flavins and both [Ni–Fe] and [Fe–Fe] hydrogenases.
Appl Microbiol Biotechnol, 2014, 73 (6), 7759 – 2707. DOI: 10.1007/s00253-013-5208-z HAL: hal-01494493v1

Francesco Oteri, Marc Baaden, Elisabeth Lojou, Sophie Sacquin-Mora.
Multiscale Simulations Give Insight into the Hydrogen In and Out Pathways of [NiFe]-Hydrogenases from Aquifex aeolicus and Desulfovibrio fructosovorans.
J. Phys. Chem. B, 2014, 118 (48), 13800 – 13811. DOI: 10.1021/jp5089965 HAL: hal-01588442v1

C. Hamon, A. Ciaccafava, P. Infossi, R. Puppo, P. Even-Hernandez, E. Lojou, V. Marchi.
Synthesis and enzymatic photo-activity of an O 2 tolerant hydrogenase–CdSe@CdS quantum rod bioconjugate.
Chem. Commun., 2014, 50 (8), 4989 – 4992. DOI: 10.1039/C3CC49368G HAL: hal-01114425v1

Vincent Fourmond, Claudio Greco, Kateryna Sybirna, Carole Baffert, Po-Hung Wang, Pierre Ezanno, Marco Montefiori, Maurizio Bruschi, Isabelle Meynial-Salles, Philippe Soucaille, Jochen Blumberger, Hervé Bottin, Luca De Gioia, Christophe Léger.
The oxidative inactivation of FeFe hydrogenase reveals the flexibility of the H-cluster.
Nature Chem, 2014, 130 (4), 4533 – 342. DOI: 10.1038/nchem.1892 HAL: hal-01481520v1

Francesco Oteri, Alexandre Ciaccafava, Anne de Poulpiquet, Marc Baaden, Elisabeth Lojou, Sophie Sacquin-Mora.
The weak, fluctuating, dipole moment of membrane-bound hydrogenase from Aquifex aeolicus accounts for its adaptability to charged electrodes.
Phys. Chem. Chem. Phys., 2014, 21 (23), 11318 – 11322. DOI: 10.1039/C4CP00510D HAL: hal-01493492v1



2013

Kateryna Sybirna, Pierre Ezanno, Carole Baffert, Christophe Léger, Hervé Bottin.
Arginine171 of Chlamydomonas reinhardtii [Fe–Fe] hydrogenase HydA1 plays a crucial role in electron transfer to its catalytic center.
International Journal of Hydrogen Energy, 2013, 38 (7), 2998 – 3002. DOI: 10.1016/j.ijhydene.2012.12.078

Katarzyna Szot, Anne de Poulpiquet, Alexandre Ciaccafava, Helena Marques, Martin Jönsson-Niedziolka, Joanna Niedziolka-Jönsson, Frank Marken, Elisabeth Lojou, Marcin Opallo.
Carbon nanoparticulate films as effective scaffolds for mediatorless bioelectrocatalytic hydrogen oxidation.
Electrochimica Acta, 2013, 111, 434 – 440. DOI: 10.1016/j.electacta.2013.08.001

Toshiko Miyake, Maurizio Bruschi, Ugo Cosentino, Carole Baffert, Vincent Fourmond, Christophe Léger, Giorgio Moro, Luca De Gioia, Claudio Greco.
Does the environment around the H-cluster allow coordination of the pendant amine to the catalytic iron center in [FeFe] hydrogenases? Answers from theory.
J Biol Inorg Chem, 2013, 13 (6), 3580 – 700. DOI: 10.1007/s00775-013-1014-4

Anne De Poulpiquet, Alexandre Ciaccafava, Katarzyna Szot, Baptiste Pillain, Pascale Infossi, Marianne Guiral, Marcin Opallo, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Exploring Properties of a Hyperthermophilic Membrane-Bound Hydrogenase at Carbon Nanotube Modified Electrodes for a Powerful H 2 /O 2 Biofuel Cell.
Electroanalysis, 2013, 284 (3), 465 – 695. DOI: 10.1002/elan.201200405

Alexandre Ciaccafava, Cyrille Hamon, Pascale Infossi, Valérie Marchi, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Light-induced reactivation of O2-tolerant membrane-bound [Ni–Fe] hydrogenase from the hyperthermophilic bacterium Aquifex aeolicus under turnover conditions.
Phys. Chem. Chem. Phys., 2013, 15 (39), 16463 – 16467. DOI: 10.1039/c3cp52596a HAL: hal-00860457v1

Abbas Abou Hamdan, Bénédicte Burlat, Oscar Gutiérrez-Sanz, Pierre-Pol Liebgott, Carole Baffert, Antonio L De Lacey, Marc Rousset, Bruno Guigliarelli, Christophe Léger, Sébastien Dementin.
O2-independent formation of the inactive states of NiFe hydrogenase.
Nat Chem Biol, 2013, 118 (1), 12989 – 17. DOI: 10.1038/NCHEMBIO.1110 HAL: hal-01556905v1

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Thomas Lautier, Abbas Abou Hamdan, Sébastien Dementin, Philippe Soucaille, Isabelle Meynial-Salles, Hervé Bottin, Christophe Léger.
Steady-State Catalytic Wave-Shapes for 2-Electron Reversible Electrocatalysts and Enzymes.
J. Am. Chem. Soc., 2013, 135 (10), 3926 – 3938. DOI: 10.1021/ja311607s HAL: hal-01268145v1

Vincent Fourmond, Carole Baffert, Kateryna Sybirna, Sébastien Dementin, Abbas Abou-Hamdan, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
The mechanism of inhibition by H2 of H2-evolution by hydrogenases.
Chem. Commun., 2013, 109 (61), 19916 –. DOI: 10.1039/C3CC43297A HAL: hal-01268212v1



2012

A. Ciaccafava, A. De Poulpiquet, P. Infossi, S. Robert, R. Gadiou, M.T. Giudici-Orticoni, S. Lecomte, E. Lojou.
A friendly detergent for H2 oxidation by Aquifex aeolicus membrane-bound hydrogenase immobilized on graphite and Self-Assembled-Monolayer-modified gold electrodes.
Electrochimica Acta, 2012, 82, 115 – 125. DOI: 10.1016/j.electacta.2012.03.034

A. Ciaccafava, A. De Poulpiquet, V. Techer, M.T. Giudici-Orticoni, S. Tingry, C. Innocent, E. Lojou.
An innovative powerful and mediatorless H2/O2 biofuel cell based on an outstanding bioanode.
Electrochemistry Communications, 2012, 23, 25 – 28. DOI: 10.1016/j.elecom.2012.06.035 HAL: hal-01690641v1

Carole Baffert, Kateryna Sybirna, Pierre Ezanno, Thomas Lautier, Viviane Hajj, Isabelle Meynial-Salles, Philippe Soucaille, Hervé Bottin, Christophe Léger.
Covalent Attachment of FeFe Hydrogenases to Carbon Electrodes for Direct Electron Transfer.
Anal. Chem., 2012, 84 (18), 7999 – 8005. DOI: 10.1021/ac301812s HAL: hal-01268277v1

Alexandre Ciaccafava, Pascale Infossi, Marianne Ilbert, Marianne Guiral, Sophie Lecomte, Marie Thérèse Giudici-Orticoni, Elisabeth Lojou.
Electrochemistry, AFM, and PM-IRRA Spectroscopy of Immobilized Hydrogenase: Role of a Hydrophobic Helix in Enzyme Orientation for Efficient H2 Oxidation.
Angew. Chem. Int. Ed., 2012, 51 (4), 953 – 956. DOI: 10.1002/anie.201107053

A. Abou Hamdan, P.-P. Liebgott, V. Fourmond, O. Gutierrez-Sanz, A. L. De Lacey, P. Infossi, M. Rousset, S. Dementin, C. Leger.
Relation between anaerobic inactivation and oxygen tolerance in a large series of NiFe hydrogenase mutants.
Proceedings of the National Academy of Sciences, 2012, 76 (1-2), 141 – 19921. DOI: 10.1073/pnas.1212258109 HAL: hal-01825482v1

Abbas Abou Hamdan, Sébastien Dementin, Pierre-Pol Liebgott, Oscar Gutierrez-Sanz, Pierre Richaud, Antonio L. De Lacey, Marc Rousset, Patrick Bertrand, Laurent Cournac, Christophe Léger.
Understanding and Tuning the Catalytic Bias of Hydrogenase.
J. Am. Chem. Soc., 2012, 134 (20), 8368 – 8371. DOI: 10.1021/ja301802r HAL: hal-00748324v1



2011

Vincent Fourmond, Sigolène Canaguier, Benjamin Golly, Martin J. Field, Marc Fontecave, Vincent Artero.
A nickel–manganese catalyst as a biomimic of the active site of NiFe hydrogenases: a combined electrocatalytical and DFT mechanistic study.
Energy Environ. Sci., 2011, 76 (7), 141 –. DOI: 10.1039/C0EE00736F HAL: hal-01139836v1

Carole Baffert, Luca Bertini, Thomas Lautier, Claudio Greco, Kateryna Sybirna, Pierre Ezanno, Emilien Etienne, Philippe Soucaille, Patrick Bertrand, Hervé Bottin, Isabelle Meynial-Salles, Luca De Gioia, Christophe Léger.
CO Disrupts the Reduced H-Cluster of FeFe Hydrogenase. A Combined DFT and Protein Film Voltammetry Study.
J. Am. Chem. Soc., 2011, 133 (7), 2096 – 2099. DOI: 10.1021/ja110627b

A. Ciaccafava, M. Alberola, S. Hameury, P. Infossi, M.T. Giudici-Orticoni, E. Lojou.
Hydrogen bioelectrooxidation in ionic liquids: From cytochrome c3 redox behavior to hydrogenase activity.
Electrochimica Acta, 2011, 56 (9), 3359 – 3368. DOI: 10.1016/j.electacta.2010.12.104

Elisabeth Lojou.
Hydrogenases as catalysts for fuel cells: Strategies for efficient immobilization at electrode interfaces.
Electrochimica Acta, 2011, 56 (28), 10385 – 10397. DOI: 10.1016/j.electacta.2011.03.002

Pierre-Pol Liebgott, Antonio L. de Lacey, Bénédicte Burlat, Laurent Cournac, Pierre Richaud, Myriam Brugna, Victor M. Fernandez, Bruno Guigliarelli, Marc Rousset, Christophe Léger, Sébastien Dementin.
Original Design of an Oxygen-Tolerant [NiFe] Hydrogenase: Major Effect of a Valine-to-Cysteine Mutation near the Active Site.
J. Am. Chem. Soc., 2011, 133 (4), 986 – 997. DOI: 10.1021/ja108787s HAL: hal-01977599v1

Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 – 10221. DOI: 10.1021/ja202615a HAL: hal-00677402v1

Thomas Lautier, Pierre Ezanno, Carole Baffert, Vincent Fourmond, Laurent Cournac, Juan C. Fontecilla-Camps, Philippe Soucaille, Patrick Bertrand, Isabelle Meynial-Salles, Christophe Léger.
The quest for a functional substrate access tunnel in FeFe hydrogenase.
Faraday Discuss., 2011, 304, 55 – 407. DOI: 10.1039/c004099c

Pierre-Pol Liebgott, Sébastien Dementin, Christophe Léger, Marc Rousset.
Towards engineering O2-tolerance in [Ni–Fe] hydrogenases.
Energy Environ. Sci., 2011, 128 (1), 5209 – 41. DOI: 10.1039/c0ee00093k HAL: hal-01977604



2010

Pascale Infossi, Elisabeth Lojou, Jean-Paul Chauvin, Gaetan Herbette, Myriam Brugna, Marie-Thérèse Giudici-Orticoni.
Aquifex aeolicus membrane hydrogenase for hydrogen biooxidation: Role of lipids and physiological partners in enzyme stability and activity.
International Journal of Hydrogen Energy, 2010, 35 (19), 10778 – 10789. DOI: 10.1016/j.ijhydene.2010.02.054 HAL: hal-00686860v1

Vincent Fourmond, Pierre-André Jacques, Marc Fontecave, Vincent Artero.
H2 Evolution and Molecular Electrocatalysts: Determination of Overpotentials and Effect of Homoconjugation.
Inorg. Chem., 2010, 49 (22), 10338 – 10347. DOI: 10.1021/ic101187v HAL: hal-01069160v1

Fanny Leroux, Pierre-Pol Liebgott, Laurent Cournac, Pierre Richaud, Arlette Kpebe, Bénedicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Christophe Léger, Marc Rousset.
Is engineering O2-tolerant hydrogenases just a matter of reproducing the active sites of the naturally occurring O2-resistant enzymes?.
International Journal of Hydrogen Energy, 2010, 35 (19), 10770 – 10777. DOI: 10.1016/j.ijhydene.2010.02.071 HAL: hal-01977635v1

Maria-Eirini Pandelia, Vincent Fourmond, Pascale Tron-Infossi, Elisabeth Lojou, Patrick Bertrand, Christophe Léger, Marie-Thérèse Giudici-Orticoni, Wolfgang Lubitz.
Membrane-Bound Hydrogenase I from the Hyperthermophilic Bacterium Aquifex aeolicus: Enzyme Activation, Redox Intermediates and Oxygen Tolerance.
J. Am. Chem. Soc., 2010, 132 (20), 6991 – 7004. DOI: 10.1021/ja910838d HAL: hal-00686880v1

Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 275 (1), 113 – 70. DOI: 10.1038/nchembio.276 HAL: hal-01977629

Alexandre Ciaccafava, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou.
Stabilization Role of a Phenothiazine Derivative on the Electrocatalytic Oxidation of Hydrogen via Aquifex aeolicus Hydrogenase at Graphite Membrane Electrodes.
Langmuir, 2010, 26 (23), 18534 – 18541. DOI: 10.1021/la103714n

Vincent Fourmond, Pascale Infossi, Marie-Thérèse Giudici-Orticoni, Patrick Bertrand, Christophe Léger.
“Two-Step” Chronoamperometric Method for Studying the Anaerobic Inactivation of an Oxygen Tolerant NiFe Hydrogenase.
J. Am. Chem. Soc., 2010, 132 (13), 4848 – 4857. DOI: 10.1021/ja910685j HAL: hal-00677470



2009

Xiaojun Luo, Myriam Brugna, Pascale Tron-Infossi, Marie Thérèse Giudici-Orticoni, Élisabeth Lojou.
Immobilization of the hyperthermophilic hydrogenase from Aquifex aeolicus bacterium onto gold and carbon nanotube electrodes for efficient H2 oxidation.
J Biol Inorg Chem, 2009, 14 (8), 1275 – 1288. DOI: 10.1007/s00775-009-0572-y

Sébastien Dementin, Fanny Leroux, Laurent Cournac, Antonio L. de Lacey, Anne Volbeda, Christophe Léger, Bénédicte Burlat, Nicolas Martinez, Stéphanie Champ, Lydie Martin, Oliver Sanganas, Michael Haumann, Víctor M. Fernández, Bruno Guigliarelli, Ju.
Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant.
J. Am. Chem. Soc., 2009, 131 (29), 10156 – 10164. DOI: 10.1021/ja9018258



2008

É. Lojou, X. Luo, M. Brugna, N. Candoni, S. Dementin, M. T. Giudici-Orticoni.
Biocatalysts for fuel cells: efficient hydrogenase orientation for H2 oxidation at electrodes modified with carbon nanotubes.
J Biol Inorg Chem, 2008, 13 (7), 1157 – 1167. DOI: 10.1007/s00775-008-0401-8 HAL: hal-00335503

F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset, C. Leger.
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences, 2008, 53 (Pt 3), 240 – 11193. DOI: 10.1073/pnas.0803689105



2006

Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 – 5218. DOI: 10.1021/ja060233b HAL: hal-00335157v1

M GUIRAL, P TRON, V BELLE, C AUBERT, C LEGER, B GUIGLIARELLI, M GIUDICIORTICONI.
Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties.
International Journal of Hydrogen Energy, 2006, 31 (11), 1424 – 1431. DOI: 10.1016/j.ijhydene.2006.06.007 HAL: hal-00335435



2005

Élisabeth Lojou, Marie-Thérèse Giudici-Orticoni, Pierre Bianco.
Hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: electrocatalysis of the hydrogen production/consumption reactions at carbon electrodes.
Journal of Electroanalytical Chemistry, 2005, 577 (1), 79 – 86. DOI: 10.1016/j.jelechem.2004.11.016



2004

É. Lojou, P. Bianco.
Electrocatalytic Reactions at Hydrogenase-Modified Electrodes and Their Applications to Biosensors: From the Isolated Enzymes to the Whole Cells.
Electroanalysis, 2004, 16 (1314), 1093 – 1100. DOI: 10.1002/elan.200403002

Christophe Léger, Sébastien Dementin, Patrick Bertrand, Marc Rousset, Bruno Guigliarelli.
Inhibition and Aerobic Inactivation Kinetics of Desulfovibrio fructosovorans NiFe Hydrogenase Studied by Protein Film Voltammetry.
J. Am. Chem. Soc., 2004, 126 (38), 12162 – 12172. DOI: 10.1021/ja046548d HAL: hal-00336069



2003

Marianne Brugna-Guiral, Pascale Tron, Wolfgang Nitschke, Karl-Otto Stetter, Benedicte Burlat, Bruno Guigliarelli, Mireille Bruschi, Marie Thérèse Giudici-Orticoni.
[NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics.
Extremophiles, 2003, 139 (2), 139 – 157. DOI: 10.1007/s00792-002-0306-3



2002

E. Lojou, M. C. Durand, A. Dolla, P. Bianco.
Hydrogenase Activity Control at Desulfovibrio vulgaris Cell-Coated Carbon Electrodes: Biochemical and Chemical Factors Influencing the Mediated Bioelectrocatalysis.
Electroanalysis, 2002, 14 (13), 913 –. DOI: 10.1002/1521-4109(200207)14:133.0.CO;2-N