Electron Paramagnetic Resonance Facility
in Life Sciences at the Chemistry-Biology interface

Publications

2025

Wouter Versantvoort, Rainer Hienerwadel, Christina Ferousi, Pieter van der Velden, Catherine Berthomieu, Laura van Niftrik, Frauke Baymann.
Spectroscopic insights into the mechanism of anammox hydrazine synthase.
Journal of Biological Chemistry, 2025, 301 (11), 110771 –. DOI: 10.1016/j.jbc.2025.110771 HAL: hal-05365182

Alessio Bonucci, Tingting Wang, Hakima Baroudi, Emilien Etienne, Guillaume Gerbaud, Elisabetta Mileo, Goetz Parsiegla, Takahisa Yamato, Brigitte Gontero, Hélène Launay, Valérie Belle, Véronique Receveur‐Bréchot.
Hidden protein disorder: Deciphering the structural organisation and dynamics of a non‐canonical CP12 from the diatom Thalassiosira pseudonana.
The FEBS Journal, 2025. DOI: 10.1111/febs.70187 HAL: hal-05169659

Ana Rita Oliveira, Guilherme Vilela-Alves, Cristiano Mota, Christophe Léger, Vincent Fourmond, Frédéric Biaso, Bruno Guigliarelli, Maria João Romão, Inês A. Cardoso Pereira.
The Role of Selenocysteine in Catalysis and Oxygen Tolerance of a W-Dependent Formate Dehydrogenase.
ACS Catal., 2025, 15 (15), 12627 – 12639. DOI: 10.1021/acscatal.5c02382 HAL: hal-05206805v1

Maxime Bizet, Áron Balázsi, Frédéric Biaso, Deborah Byrne, Emilien Etienne, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Gilles Truan, Guillaume Gerbaud, Tamás Kálai, Marlène Martinho.
Expanding the Diversity of Nitroxide‐Based Paramagnetic Probes Conjugated to Non‐Canonical Amino Acids for Sdsl‐Epr Applications.
ChemBioChem, 2025. DOI: 10.1002/cbic.202500064 HAL: hal-04990674

Sylvain Gervason, Rafal Dutkiewicz, Kristian Want, Rania Benazza, Rémi Mor-Gautier, Aneta Grabinska-Rogala, Christina Sizun, Oscar Hernandez-Alba, Sarah Cianferani, Bruno Guigliarelli, Bénédicte Burlat, Benoit D’Autréaux.
The ISC machinery assembles [2Fe–2S] clusters by formation and fusion of [1Fe–1S] precursors.
Nat Chem Biol, 2025. DOI: 10.1038/s41589-024-01818-8 HAL: hal-05308233


2024

Maxime Bizet, Áron Balázsi, Frédéric Biaso, Deborah Byrne, Emilien Etienne, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Gilles Truan, Guillaume Gerbaud, Tamás Kálai, Marlène Martinho.
Expanding the diversity of nitroxide-based paramagnetic probes conjugated to non-canonical amino acids for SDSL-EPR applications.
BioaRxiv, 2024. DOI: 10.1101/2024.12.18.629154 HAL: hal-04958061

Ahmed Djeghader, Julia Rendon, Frédéric Biaso, Guillaume Gerbaud, Wolfgang Nitschke, Barbara Schoepp-Cothenet, Tewfik Soulimane, Stéphane Grimaldi.
Structural and Spectroscopic Investigations of pH-Dependent Mo(V) Species in a Bacterial Sulfite-Oxidizing Enzyme.
Inorg. Chem., 2024. DOI: 10.1021/acs.inorgchem.4c02584 HAL: hal-04801647

Annalisa Pierro, Alessio Bonucci, Axel Magalon, Valérie Belle, Elisabetta Mileo.
Impact of Cellular Crowding on Protein Structural Dynamics Investigated by EPR Spectroscopy.
Chem. Rev., 2024, 124 (17), 9873 – 9898. DOI: 10.1021/acs.chemrev.3c00951 HAL: hal-04708281

Jacek Switala, Lynda Donald, Anabella Ivancich.
A remarkable peroxidase-like behavior of the catalase KatA from the pathogenic bacteria Helicobacter pylori: The oxidation reaction with formate as substrate and the stabilization of an [Fe(IV) = O Trp•] intermediate assessed by multifrequency EPR spectroscopy.
Journal of Inorganic Biochemistry, 2024, 257, 112594. DOI: 10.1016/j.jinorgbio.2024.112594 HAL: hal-04586002

Cécile Mons, Myriam Salameh, Thomas Botzanowski, Martin Clémancey, Pierre Dorlet, Cindy Vallières, Stéphane Erb, Laurence Vernis, Olivier Guittet, Michel Lepoivre, Meng-Er Huang, Sarah Cianferani, Jean-Marc Latour, Geneviève Blondin, Marie-Pierre Golinelli-Cohen.
Regulations of mitoNEET by the key redox homeostasis molecule glutathione.
Journal of Inorganic Biochemistry, 2024, 255, 112535. DOI: 10.1016/j.jinorgbio.2024.112535 HAL: hal-04520876

Yasmin Ben‐Ishay, Yoav Barak, Akiva Feintuch, Olivier Ouari, Annalisa Pierro, Elisabetta Mileo, Xun‐Cheng Su, Daniella Goldfarb.
Exploring the dynamics and structure of PpiB in living Escherichia coli cells using electron paramagnetic resonance spectroscopy.
Protein Science, 2024, 33 (3), e4903. DOI: 10.1002/pro.4903 HAL: hal-04466515

Alessia Lasorsa, Hamida Merzougui, François-Xavier Cantrelle, Giuseppe Sicoli, Elian Dupré, Xavier Hanoulle, Valérie Belle, Caroline Smet-Nocca, Isabelle Landrieu.
Magnetic resonance investigation of conformational responses of tau protein to specific phosphorylation.
Biophysical Chemistry, 2024, 305, 107155. DOI: 10.1016/j.bpc.2023.107155 HAL: hal-04440623v1

Melanie Rossotti, Diletta Arceri, Pascal Mansuelle, Olivier Bornet, Anne Durand, Soufian Ouchane, Hélène Launay, Pierre Dorlet.
The green cupredoxin CopI is a multicopper protein able to oxidize Cu(I).
Journal of Inorganic Biochemistry, 2024, 254, 112503. DOI: 10.1016/j.jinorgbio.2024.112503 HAL: hal-04448188v1

Maxime Bizet, Deborah Byrne, Frédéric Biaso, Guillaume Gerbaud, Emilien Etienne, Giuseppina Briola, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Tamas Kalai, Gilles Truan, Marlene Martinho.
Structural insights into the semiquinone form of human Cytochrome P450 reductase by DEER distance measurements between a native flavin and a spin labelled non‐canonical amino acid.
Chemistry Eur J, 2024. DOI: 10.1002/chem.202304307 HAL: hal-04426634

Marlène Martinho, Diane Allegro, Emilien Etienne, Cynthia Lohberger, Alessio Bonucci, Valérie Belle, Pascale Barbier.
Structural Flexibility of Tau in Its Interaction with Microtubules as Viewed by Site-Directed Spin Labeling EPR Spectroscopy.
Methods in Molecular Biology, 2024, 2754, 55 – 75. DOI: 10.1007/978-1-0716-3629-9_3 HAL: hal-04532622v1

Xavier Kubiak, Ivan Polsinelli, Leonard M. G. Chavas, Cameron D. Fyfe, Alain Guillot, Laura Fradale, Clémence Brewee, Stéphane Grimaldi, Guillaume Gerbaud, Aurélien Thureau, Pierre Legrand, Olivier Berteau, Alhosna Benjdia.
Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme.
Nat Chem Biol, 2024. DOI: 10.1038/s41589-023-01493-1 HAL: hal-04371487

Margot Di Cesare, Elise Kaplan, Julia Rendon, Guillaume Gerbaud, Sepideh Valimehr, Alexia Gobet, Thu-Anh Thi Ngo, Vincent Chaptal, Pierre Falson, Marlène Martinho, Pierre Dorlet, Eric Hanssen, Jean-Michel Jault, Cédric Orelle.
The transport activity of the multidrug ABC transporter BmrA does not require a wide separation of the nucleotide-binding domains.
Journal of Biological Chemistry, 2024, 300 (1), 105546. DOI: 10.1016/j.jbc.2023.105546 HAL: hal-04435310

Ana Rita Oliveira, Cristiano Mota, Guilherme Vilela-Alves, Rita Rebelo Manuel, Neide Pedrosa, Vincent Fourmond, Kateryna Klymanska, Christophe Léger, Bruno Guigliarelli, Maria João Romão, Inês A. Cardoso Pereira.
An allosteric redox switch involved in oxygen protection in a CO2 reductase.
Nat Chem Biol, 2024, 20 (1), 111-119. DOI: 10.1038/s41589-023-01484-2 HAL: hal-04383526 (dataset available here)

Pierre Dorlet, Melanie Rossotti.
Un système original de détoxication du cuivre chez les bactéries.
Actualité Chimique, 2024, 499, 37. HAL: hal-04942126v1


2023

Duc-Duy Vu, Alessio Bonucci, Manon Brenière, Metztli Cisneros-Aguirre, Philippe Pelupessy, Ziqing Wang, Ludovic Carlier, Guillaume Bouvignies, Patricia Cortes, Aneel K. Aggarwal, Martin Blackledge, Zoher Gueroui, Valérie Belle, Jeremy M. Stark, Mauro Modesti, Fabien Ferrage.
Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro.
BioRxiv, 2023. DOI: 10.1101/2023.07.12.548668 HAL: hal-04267167v1

Annalisa Pierro, Ketty Concetta Tamburrini, Hugo Leguenno, Guillaume Gerbaud, Emilien Etienne, Bruno Guigliarelli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR.
iScience, 2023, 26 (10), 107855. DOI: 10.1016/j.isci.2023.107855 HAL: hal-04233485v1

Guillaume Gerbaud, Brice Barbat, Mathilde Tribout, Emilien Etienne, Valérie Belle, Badreddine Douzi, Romé Voulhoux, Alessio Bonucci.
Refining the Dynamic Network of T2SS Endopilus Tip Heterocomplex Combining cw‐EPR and Nitroxide–Gd III Distance Measurements.
ChemBioChem, 2023, 24 (15), e2023000. DOI: 10.1002/cbic.202300099 HAL: hal-04210232v1

Pierre Nabokoff, Guillaume Brulay, Cyrielle Dol, Guillaume Gerbaud, Bruno Guigliarelli, Emilien Etienne, Emily Bloch, Fabio Ziarelli, Eric Besson, Stéphane Gastaldi.
Synthesis and Characterization of Face-to-Face Arylsulfanyl and Phenoxyl Radicals Embedded in Mesoporous Silicas.
J. Phys. Chem. C, 2023, 127 (20), 9699 – 9706. DOI: 10.1021/acs.jpcc.3c01550 HAL: hal-04114864v1

D. Marin, G. Gerbaud, O. Margeat, F. Ziarelli, F. Ferrer, O. Ouari, A. Campos, S. Bertaina, A. Savoyant.
Magnetic functionalization of ZnO nanoparticles surfaces via optically generated methyl radicals.
J. Chem. Phys., 2023, 158 (18). DOI: 10.1063/5.0152015 HAL: hal-04115234v1

Laura Prioretti, Giulia D’Ermo, Pascale Infossi, Arlette Kpebe, Régine Lebrun, Marielle Bauzan, Elisabeth Lojou, Bruno Guigliarelli, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
Carbon Fixation in the Chemolithoautotrophic Bacterium Aquifex aeolicus Involves Two Low-Potential Ferredoxins as Partners of the PFOR and OGOR Enzymes.
Life, 2023, 13 (3), 627. DOI: 10.3390/life13030627 HAL: hal-04071170v1

Emilien Etienne, Annalisa Pierro, Ketty C. Tamburrini, Alessio Bonucci, Elisabetta Mileo, Marlène Martinho, Valérie Belle.
Guidelines for the Simulations of Nitroxide X-Band cw EPR Spectra from Site-Directed Spin Labeling Experiments Using SimLabel.
Molecules, 2023, 28 (3), 1348. DOI: 10.3390/molecules28031348 HAL: hal-03971418v1

Alejandro Villalta, Batoul Srour, Audrey Lartigue, Martin Clémancey, Deborah Byrne, Florence Chaspoul, Antoine Loquet, Bruno Guigliarelli, Geneviève Blondin, Chantal Abergel, Bénédicte Burlat.
Evidence for [2Fe-2S] 2+ and Linear [3Fe-4S] 1+ Clusters in a Unique Family of Glycine/Cysteine-Rich Fe-S Proteins from Megavirinae Giant Viruses.
J. Am. Chem. Soc., 2023, 145 (5), 2733–2738. DOI: 10.1021/jacs.2c10484 HAL: hal-03967087v1


2022

Annalisa Pierro, Alessio Bonucci, Davide Normanno, Mireille Ansaldi, Eric Pilet, Olivier Ouari, Bruno Guigliarelli, Emilien Etienne, Guillaume Gerbaud, Axel Magalon, Valérie Belle, Elisabetta Mileo.
Probing Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide‐Based EPR Spectroscopy.
Chemistry A European J, 2022. DOI: 10.1002/chem.202202249 HAL: hal-03836431

Batoul Srour, Sylvain Gervason, Maren Hellen Hoock, Beata Monfort, Kristian Want, Djabir Larkem, Nadine Trabelsi, Gautier Landrot, Andrea Zitolo, Emiliano Fonda, Emilien Etienne, Guillaume Gerbaud, Christina Sophia Müller, Jonathan Oltmanns, Jesse B. Gordon, Vishal Yadav, Malgorzata Kleczewska, Marcin Jelen, Michel B. Toledano, Rafal Dutkiewicz, David P. Goldberg, Volker Schünemann, Bruno Guigliarelli, Bénédicte Burlat, Christina Sizun, Benoit D’Autréaux.
Iron Insertion at the Assembly Site of the ISCU Scaffold Protein Is a Conserved Process Initiating Fe–S Cluster Biosynthesis.
J. Am. Chem. Soc., 2022. DOI: 10.1021/jacs.2c06338 HAL: hal-03837292v1

Maryam Seif-Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
Comparative analysis of the mena- and demethylmena-(semi)quinone binding mode to the quinol oxidation site of E. coli nitrate reductase A.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, 148750. DOI: 10.1016/j.bbabio.2022.148750

Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle.
EPR-MRS site: EPR spectroscopy for the study of biomolecules.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, Supplement, 29. DOI: 10.1016/j.bbabio.2022.148685 HAL: hal-03805551v1

Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle.
MOSBRI- A New European Research Infrastructure for Molecular Biophysics.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, Supplement, 28. DOI: 10.1016/j.bbabio.2022.148684 HAL: hal-03805560v1

Eric Pilet, Maryam Seif-Eddine, Farida Seduk, Julia Rendon, Bruno Guigliarelli, Stéphane Grimaldi, Axel Magalon.
New insights in quinone reactivity in the nitrate reductase A from E. coli.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, 148814. DOI: 10.1016/j.bbabio.2022.148814

Ana Rita Oliveira, Cristiano Mota, Kateryna Klymanska, Frédéric Biaso, Maria João Romão, Bruno Guigliarelli, Inês Cardoso Pereira.
Spectroscopic and Structural Characterization of Reduced Desulfovibrio vulgaris Hildenborough W-FdhAB Reveals Stable Metal Coordination during Catalysis.
ACS Chem. Biol., 2022, 17 (7), 1901 – 1909. DOI: 10.1021/acschembio.2c00336

Cameron D. Fyfe, Noelia Bernardo-García, Laura Fradale, Stéphane Grimaldi, Alain Guillot, Clémence Brewee, Leonard M. G. Chavas, Pierre Legrand, Alhosna Benjdia, Olivier Berteau.
Crystallographic snapshots of a B12-dependent radical SAM methyltransferase.
Nature, 2022. DOI: 10.1038/s41586-021-04355-9 HAL: hal-03808863v1

Eric Besson, Aurelien Vebr, Fabio Ziarelli, Emily Bloch, Guillaume Gerbaud, Séverine Queyroy, Pierre Thureau, Stéphane Viel, Stéphane Gastaldi.
Investigating the efficiency of silica materials with wall-embedded nitroxide radicals for dynamic nuclear polarisation NMR.
Phys. Chem. Chem. Phys., 2022, 24 (41), 25279 – 25286. DOI: 10.1039/d2cp02872g HAL: hal-03844800

Miguel Á. Casado-Combreras, Francisco Rivero-Rodríguez, Carlos A. Elena-Real, Dmitry Molodenskiy, Antonio Díaz-Quintana, Marlène Martinho, Guillaume Gerbaud, Katiuska González-Arzola, Adrián Velázquez-Campoy, Dmitri Svergun, Valérie Belle, Miguel A. De la Rosa, Irene Díaz-Moreno.
PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ.
Computational and Structural Biotechnology Journal, 2022, 20, 3695 – 3707. DOI: 10.1016/j.csbj.2022.07.009 HAL: hal-03965171v1

Jérôme Gout, Floriane Meuris, Alain Desbois, Pierre Dorlet.
In vitro coordination of Fe-protoheme with amyloid β is non-specific and exhibits multiple equilibria.
Journal of Inorganic Biochemistry, 2022, 227, 111664. DOI: 10.1016/j.jinorgbio.2021.111664 HAL: hal-03460461


2021

Annalisa Pierro, Alessio Bonucci, Valérie Belle, Elisabetta Mileo.
La RPE in cellula : étudier la dynamique des protéines dans leur habitat.
Actualité Chimique, 2021, 468, 38-41. (voir / see) HAL: hal-03855076v1

Anne Durand, Mélanie Fouesnard, Marie-Line Bourbon, Anne-Soisig Steunou, Elisabeth Lojou, Pierre Dorlet, Soufian Ouchane.
A periplasmic cupredoxin with a green CuT1.5 center is involved in bacterial copper tolerance.
Metallomics, 2021, 13 (12), mfab067. DOI: 10.1093/mtomcs/mfab067 HAL: hal-03453712v1

Sinan Al-Attar, Julia Rendon, Marlon Sidore, Jean-Pierre Duneau, Farida Seduk, Frédéric Biaso, Stéphane Grimaldi, Bruno Guigliarelli, Axel Magalon.
Gating of Substrate Access and Long-Range Proton Transfer in Escherichia coli Nitrate Reductase A: The Essential Role of a Remote Glutamate Residue.
ACS Catal., 2021, 14303 – 14318. DOI: 10.1021/acscatal.1c03988 HAL: hal-03511729v1

Rodrigo Arias-Cartin, Alexandre Uzel, Farida Seduk, Guillaume Gerbaud, Fabien Pierrel, Marianne Broc, Régine Lebrun, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi, Anne Walburger.
Identification and characterization of a non-canonical menaquinone-linked formate dehydrogenase.
Journal of Biological Chemistry, 2021, 101384. DOI: 10.1016/j.jbc.2021.101384 HAL: hal-03431446v1

Christelle Caux, Bruno Guigliarelli, Corinne Vivès, Frédéric Biaso, Marius Horeau, Hawra Hassoune, Isabelle Petit-Hartlein, Céline Juillan-Binard, Stephane Torelli, Franck Fieschi, Vincent Nivière.
Membrane-Bound Flavocytochrome MsrQ Is a Substrate of the Flavin Reductase Fre in Escherichia coli.
ACS Chem. Biol., 2021. DOI: 10.1021/acschembio.1c00613 HAL: hal-03320876v1

F. Torricella, A. Pierro, E. Mileo, V. Belle, A. Bonucci.
Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies.
Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics, 2021, 1869 (7), 140653. DOI: 10.1016/j.bbapap.2021.140653 HAL: hal-03441104v1

Cheryle N. Beuning, Luca J. Zocchi, Kyangwi P. Malikidogo, Charlène Esmieu, Pierre Dorlet, Debbie C. Crans, Christelle Hureau.
Measurement of Interpeptidic Cu(II) Exchange Rate Constants of Cu(II)-Amyloid-β Complexes to Small Peptide Motifs by Tryptophan Fluorescence Quenching.
Inorg. Chem., 2021, 60, 7650-7659. DOI: 10.1021/acs.inorgchem.0c03555 HAL: hal-03380483

Umberto Contaldo, Bruno Guigliarelli, Julien Perard, Clara Rinaldi, Alan Le Goff, Christine Cavazza.
Efficient Electrochemical CO2/CO Interconversion by an Engineered Carbon Monoxide Dehydrogenase on a Gas-Diffusion Carbon Nanotube-Based Bioelectrode.
ACS Catal., 2021, 11 (9), 5808 – 5817. DOI: 10.1021/acscatal.0c05437 HAL: hal-03258521v1

Majid Haddad Momeni, Folmer Fredslund, Bastien Bissaro, Olanrewaju Raji, Thu V. Vuong, Sebastian Meier, Tine Sofie Nielsen, Vincent Lombard, Bruno Guigliarelli, Frédéric Biaso, Mireille Haon, Sacha Grisel, Bernard Henrissat, Ditte Hededam Welner, Emma R. Master, Jean-Guy Berrin, Maher Abou Hachem.
Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs.
Nat Commun, 2021, 12 (1). DOI: 10.1038/s41467-021-22372-0 HAL: hal-03234953v1

Anouchka Fiévet, Meriem Merrouch, Gaël Brasseur, Danaé Eve, Emanuele G. Biondi, Odile Valette, Sofia R. Pauleta, Alain Dolla, Zorah Dermoun, Bénédicte Burlat, Corinne Aubert.
OrpR is a σ 54 ‐dependent activator using an iron‐sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough.
Mol. Microbiol., 2021, 116 (1), 231 – 244. DOI: 10.1111/mmi.14705 HAL: hal-03204123v1

Karl J. Koebke, Toni Kühl, Elisabeth Lojou, Borries Demeler, Barbara Schoepp-Cothenet, Olga Iranzo, Vincent L. Pecoraro, Anabella Ivancich.
The pH‐Induced Selectivity Between Cysteine or Histidine Coordinated Heme in an Artificial α‐Helical Metalloprotein.
Angew. Chem. Int. Ed., 2021, 60 (8), 3974 – 3978. DOI: 10.1002/anie.202012673 HAL: hal-03017501


2020

Mark J Burton, Joel Cresser-Brown, Morgan Thomas, Nicola Portolano, Jaswir Basran, Samuel L Freeman, Hanna Kwon, Andrew R. Bottrill, Manuel J Llansola-Portoles, Andrew A Pascal, Rebekah Jukes-Jones, Tatyana Chernova, Ralf Schmid, Noel W Davies, Nina M Storey, Pierre Dorlet, Peter C. E. Moody, John S Mitcheson, Emma L Raven.
Discovery of a heme-binding domain in a neuronal voltage-gated potassium channel.
J. Biol. Chem., 2020, 295 (38), 13277 – 13286. DOI: 10.1074/jbc.RA120.014150 HAL: hal-02928173v1

Ahmed Djeghader, Mélanie Rossotti, Saleh Abdulkarim, Frédéric Biaso, Guillaume Gerbaud, Wolfgang Nitschke, Barbara Schoepp-Cothenet, Tewfik Soulimane, Stephane Grimaldi.
Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase.
Chem. Commun., 2020, 56 (68), 9850 – 9853. DOI: 10.1039/D0CC03634J HAL: hal-02930401

Annalisa Pierro, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Stefano Ciurli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
Nickel and GTP Modulate Helicobacter pylori UreG Structural Flexibility.
Biomolecules, 2020, 10 (7), 1062. DOI: 10.3390/biom10071062 HAL: hal-02902916v1

Maryam Seif Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
1,2H hyperfine spectroscopy and DFT modeling unveil the demethylmenasemiquinone binding mode to E. coli nitrate reductase A (NarGHI).
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2020, 1861 (8), 148203. DOI: 10.1016/j.bbabio.2020.148203 HAL: hal-02909846

Ana Rita Oliveira, Cristiano Mota, Cláudia Mourato, Renato M. Domingos, Marino F. A. Santos, Diana Gesto, Bruno Guigliarelli, Teresa Santos-Silva, Maria João Romão, Inês A. Cardoso Pereira.
Toward the Mechanistic Understanding of Enzymatic CO2 Reduction.
ACS Catal., 2020, 10 (6), 3844 – 3856. DOI: 10.1021/acscatal.0c00086 HAL: hal-02938569

Sarmistha Bhunia, Atanu Rana, Somdatta Ghosh Dey, Anabella Ivancich, Abhishek Dey.
A designed second-sphere hydrogen-bond interaction that critically influences the O–O bond activation for heterolytic cleavage in ferric iron–porphyrin complexes.
Chem. Sci., 2020, 11 (10), 2681 – 2695. DOI: 10.1039/C9SC04388H HAL: hal-02887080v1

Alessio Bonucci, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo.
In‐Cell EPR: Progress towards Structural Studies Inside Cells.
ChemBioChem, 2020, 21 (4), 451 – 460. DOI: 10.1002/cbic.201900291 HAL: hal-02180300v1


2019

J. Zeisner, O. Pilone, L. Soriano, G. Gerbaud, H. Vezin, O. Jeannin, M. Fourmigué, B. Büchner, V. Kataev, S. Bertaina.
Coherent spin dynamics of solitons in the organic spin chain compounds ( o -DMTTF ).
Phys. Rev. B, 2019, 100 (22). DOI: 10.1103/PhysRevB.100.224414 HAL: hal-02346730

Christina Ferousi, Simon Lindhoud, Frauke Baymann, Eric R. Hester, Joachim Reimann, Boran Kartal.
Discovery of a functional, contracted heme-binding motif within a multiheme cytochrome.
J. Biol. Chem., 2019, 261 (45), 1126 – 16965. DOI: 10.1074/jbc.RA119.010568 HAL: hal-02369902v1

Eugénie Fournier, Sybille Tachon, Nicholas J. Fowler, Guillaume Gerbaud, Pascal Mansuelle, Pierre Dorlet, Sam P. De Visser, Valérie Belle, A. Jalila Simaan, Marlène Martinho.
The Hunt for the Closed Conformation of the Fruit‐Ripening Enzyme 1‐Aminocyclopropane‐1‐carboxylic Oxidase: A Combined Electron Paramagnetic Resonance and Molecular Dynamics Study.
Chem. Eur. J., 2019, 25 (60), 13766-13776. DOI: 10.1002/chem.201903003 HAL: hal-02303326v1

Sven De Causmaecker, Jeffrey S. Douglass, Andrea Fantuzzi, Wolfgang Nitschke, A. William Rutherford.
Energetics of the exchangeable quinone, QB, in Photosystem II.
Proc Natl Acad Sci USA, 2019, 1184 (39), 85 – 19463. DOI: 10.1073/pnas.1910675116 HAL: hal-02407784v1

Robert Van Lis, Sabine Brugière, Carole Baffert, Yohann Couté, Wolfgang Nitschke, Ariane Atteia.
Hybrid cluster proteins in a photosynthetic microalga.
FEBS J, 2019, 33 (1), 1870 –. DOI: 10.1111/febs.15025 HAL: hal-02284985v1

Pascale Barbier, Orgeta Zejneli, Marlène Martinho, Alessia Lasorsa, Valérie Belle, Caroline Smet-Nocca, Philipp O. Tsvetkov, François Devred, Isabelle Landrieu.
Role of Tau as a Microtubule-Associated Protein: Structural and Functional Aspects.
Front. Aging Neurosci., 2019, 11:204, 1 – 14. DOI: 10.3389/fnagi.2019.00204 HAL: hal-02279660v1

Sybille Tachon, Eugénie Fournier, Christophe Decroos, Pascal Mansuelle, Emilien Etienne, Marc Maresca, Marlène Martinho, Valérie Belle, Thierry Tron, Ariane Jalila Simaan.
Chemical Modification of 1-Aminocyclopropane Carboxylic Acid (ACC) Oxidase: Cysteine Mutational Analysis, Characterization, and Bioconjugation with a Nitroxide Spin Label.
Mol Biotechnol, 2019, 61, 650 – 662. DOI: 10.1007/s12033-019-00191-5 HAL: hal-02157289v1

Kamal Zeamari, Guillaume Gerbaud, Sandrine Grosse, Vincent Fourmond, Florence Chaspoul, Frédéric Biaso, Pascal Arnoux, Monique Sabaty, David Pignol, Bruno Guigliarelli, Bénédicte Burlat.
Tuning the redox properties of a [4Fe-4S] center to modulate the activity of Mo-bisPGD periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2019, 1860 (5), 402 – 413. DOI: 10.1016/j.bbabio.2019.01.003 HAL: hal-02106668v1

Cyrielle Dol, Guillaume Gerbaud, Bruno Guigliarelli, Emily Bloch, Stéphane Gastaldi, Eric Besson.
Modulating lifetimes and relaxation times of phenoxyl radicals through their incorporation into different hybrid nanostructures.
Phys. Chem. Chem. Phys., 2019, 172 (29), 573 – 16344. DOI: 10.1039/C9CP03052B HAL: hal-02177931v1

Nolwenn Le Breton, Sonia Longhi, Antal Rockenbauer, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle, Marlène Martinho.
Probing the dynamic properties of two sites simultaneously in a protein–protein interaction process: a SDSL-EPR study.
Phys. Chem. Chem. Phys., 2019, 21, 22584 – 22588. DOI: 10.1039/c9cp04660g HAL: hal-02341622v1

Eugénie Fournier, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Valérie Belle, Marlène Martinho.
Spin labels: spies in the heart of proteins.
Actualité Chimique, 2019, 443. (voir / see)


2018

Arlette Kpebe, Martino Benvenuti, Chloé Guendon, Amani Rebai, Victoria Fernandez, Sébastien Le Laz, Emilien Etienne, Bruno Guigliarelli, Gabriel García-Molina, Antonio L. de Lacey, Carole Baffert, Myriam Brugna.
A new mechanistic model for an O2-protected electron-bifurcating hydrogenase, Hnd from Desulfovibrio fructosovorans.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (12), 1302 – 1312. DOI: 10.1016/j.bbabio.2018.09.364 HAL: hal-01928576v1

Marlène Martinho, Diane Allegro, Isabelle Huvent, Charlotte Chabaud, Emilien Etienne, Hervé Kovacic, Bruno Guigliarelli, Vincent Peyrot, Isabelle Landrieu, Valérie Belle, Pascale Barbier.
Two Tau binding sites on tubulin revealed by thiol-disulfide exchanges.
Sci Rep, 2018, 8:13846, 1 – 11. DOI: 10.1038/s41598-018-32096-9 HAL: hal-01883844v1

Angeliki M. Andrianaki, Irene Kyrmizi, Kalliopi Thanopoulou, Clara Baldin, Elias Drakos, Sameh S. M. Soliman, Amol C. Shetty, Carrie McCracken, Tonia Akoumianaki, Kostas Stylianou, Petros Ioannou, Charalampos Pontikoglou, Helen A. Papadaki, Maria Tzardi, Valerie Belle, Emilien Etienne, Anne Beauvais, George Samonis, Dimitrios P. Kontoyiannis, Evangelos Andreakos, Vincent M. Bruno, Ashraf S. Ibrahim, Georgios Chamilos.
Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species.
Nat Commun, 2018, 11 (Suppl. 1), R106. DOI: 10.1038/s41467-018-05820-2 HAL: hal-01892640v1

Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, David Guieysse, Laurence Girbal, Vincent Fourmond, Isabelle André, Bruno Guigliarelli, Christophe Léger, Philippe Soucaille, Isabelle Meynial-Salles.
Roles of the F-domain in [FeFe] hydrogenase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (2), 69 – 77. DOI: 10.1016/j.bbabio.2017.08.010 HAL: cea-01950818

Ganesan Karthikeyan, Alessio Bonucci, Gilles Casano, Guillaume Gerbaud, Sébastien Abel, Virginie Thomé, Laurent Kodjabachian, Axel Magalon, Bruno Guigliarelli, Valérie Belle, Olivier Ouari, Elisabetta Mileo.
A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies.
Angew. Chem. Int. Ed., 2018, 57, 1366 – 1370. DOI: 10.1002/anie.201710184 HAL: hal-02000559v1


2017

Marta Palombo, Alessio Bonucci, Emilien Etienne, Stefano Ciurli, Vladimir N. Uversky, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo, Barbara Zambelli.
The relationship between folding and activity in UreG, an intrinsically disordered enzyme.
Sci Rep, 2017, 89 (1), 392. DOI: 10.1038/s41598-017-06330-9 HAL: hal-01585289v1

Emilien Etienne, Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Valérie Belle.
SimLabel: a graphical user interface to simulate continuous wave EPR spectra from site-directed spin labeling experiments.
Magn. Reson. Chem., 2017, 55 (8), 714 – 719. DOI: 10.1002/mrc.4578 HAL: hal-01444172v1


2016

Pierre Ceccaldi, Emilien Etienne, Sébastien Dementin, Bruno Guigliarelli, Christophe Léger, Bénédicte Burlat.
Mechanism of inhibition of NiFe hydrogenase by nitric oxide.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2016, 1857 (4), 454 – 461. DOI: 10.1016/j.bbabio.2016.01.014 HAL: hal-01413192v1

Nolwenn Le Breton, Tiona Adrianaivomananjaona, Guillaume Gerbaud, Emilien Etienne, Elena Bisetto, Alain Dautant, Bruno Guigliarelli, Francis Haraux, Marlène Martinho, Valérie Belle.
Dimerization interface and dynamic properties of yeast IF1 revealed by Site-Directed Spin Labeling EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2016, 1857 (1), 89 – 97. DOI: 10.1016/j.bbabio.2015.10.010 HAL: hal-01413333v1

Eric Besson, Fabio Ziarelli, Emily Bloch, Guillaume Gerbaud, Séverine Queyroy, Stéphane Viel, Stéphane Gastaldi.
Silica materials with wall-embedded nitroxides provide efficient polarization matrices for dynamic nuclear polarization NMR.
Chem. Commun., 2016, 52 (32), 5531 – 5533. DOI: 10.1039/c6cc01809b HAL: hal-01415149v1


2015

Jeremy Guérin, Nathalie Saint, Catherine Baud, Albano C. Meli, Emilien Etienne, Camille Locht, Hervé Vezin, Françoise Jacob-Dubuisson.
Dynamic interplay of membrane-proximal POTRA domain and conserved loop L6 in Omp85 transporter FhaC.
Molecular Microbiology, 2015, 98 (3), 490 – 501. DOI: 10.1111/mmi.13137 HAL: hal-01432130v1

Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 – 7. DOI: 10.3389/fmolb.2015.00021 HAL: hal-01429982v1


2014

Julien G.J. Jacques, Vincent Fourmond, Pascal Arnoux, Monique Sabaty, Emilien Etienne, Sandrine Grosse, Frédéric Biaso, Patrick Bertrand, David Pignol, Christophe Léger, Bruno Guigliarelli, Bénédicte Burlat.
Reductive activation in periplasmic nitrate reductase involves chemical modifications of the Mo-cofactor beyond the first coordination sphere of the metal ion.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2014, 1837 (2), 277 – 286. DOI: 10.1016/j.bbabio.2013.10.013 HAL: hal-01481487v1


2013

Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 – 1117. DOI: 10.1021/bc4000542 HAL: hal-01460460v1

Elisabetta Mileo, Magali Lorenzi, Jenny Erales, Sabrina Lignon, Carine Puppo, Nolwenn Le Breton, Emilien Etienne, Sylvain R. A. Marque, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
Dynamics of the intrinsically disordered protein CP12 in its association with GAPDH in the green alga Chlamydomonas reinhardtii: a fuzzy complex.
Mol. BioSyst., 2013, 1 (11), 349. DOI: 10.1039/c3mb70190e HAL: hal-01460463v1


2011

Carole Baffert, Luca Bertini, Thomas Lautier, Claudio Greco, Kateryna Sybirna, Pierre Ezanno, Emilien Etienne, Philippe Soucaille, Patrick Bertrand, Hervé Bottin, Isabelle Meynial-Salles, Luca De Gioia, Christophe Léger.
CO Disrupts the Reduced H-Cluster of FeFe Hydrogenase. A Combined DFT and Protein Film Voltammetry Study.
J. Am. Chem. Soc., 2011, 133 (7), 2096 – 2099. DOI: 10.1021/ja110627b HAL: hal-02652182v1


2010

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Émilien Étienne, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants.
Biochemistry, 2010, 49 (11), 2424 – 2432. DOI: 10.1021/bi902140e HAL: hal-00677461


2009

Jenny Erales, Magali Lorenzi, Régine Lebrun, André Fournel, Emilien Etienne, Carine Courcelle, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
A New Function of GAPDH from Chlamydomonas reinhardtii: A Thiol−Disulfide Exchange Reaction with CP12.
Biochemistry, 2009, 48 (25), 6034 – 6040. DOI: 10.1021/bi900569h HAL: hal-00429826