Biophysique des métalloprotéines et des systèmes dynamiques

Biophysics of metalloproteins and dynamic systems



Jacek Switala, Lynda Donald, Anabella Ivancich.
A remarkable peroxidase-like behavior of the catalase KatA from the pathogenic bacteria Helicobacter pylori: The oxidation reaction with formate as substrate and the stabilization of an [Fe(IV) = O Trp•] intermediate assessed by multifrequency EPR spectroscopy.
Journal of Inorganic Biochemistry, 2024, 257, 112594. DOI: 10.1016/j.jinorgbio.2024.112594 HAL: hal-04586002

Abhik Ghosh, Anabella Ivancich.
A tribute to Frances Ann Walker.
Journal of Inorganic Biochemistry, 2024, 257, 112604. DOI: 10.1016/j.jinorgbio.2024.112604 HAL: hal-04586003

Cécile Mons, Myriam Salameh, Thomas Botzanowski, Martin Clémancey, Pierre Dorlet, Cindy Vallières, Stéphane Erb, Laurence Vernis, Olivier Guittet, Michel Lepoivre, Meng-Er Huang, Sarah Cianferani, Jean-Marc Latour, Geneviève Blondin, Marie-Pierre Golinelli-Cohen.
Regulations of mitoNEET by the key redox homeostasis molecule glutathione.
Journal of Inorganic Biochemistry, 2024, 255, 112535. DOI: 10.1016/j.jinorgbio.2024.112535 HAL: hal-04520876

Yasmin Ben‐Ishay, Yoav Barak, Akiva Feintuch, Olivier Ouari, Annalisa Pierro, Elisabetta Mileo, Xun‐Cheng Su, Daniella Goldfarb.
Exploring the dynamics and structure of PpiB in living Escherichia coli cells using electron paramagnetic resonance spectroscopy.
Protein Science, 2024, 33 (3), e4903. DOI: 10.1002/pro.4903 HAL: hal-04466515

Alessia Lasorsa, Hamida Merzougui, François-Xavier Cantrelle, Giuseppe Sicoli, Elian Dupré, Xavier Hanoulle, Valérie Belle, Caroline Smet-Nocca, Isabelle Landrieu.
Magnetic resonance investigation of conformational responses of tau protein to specific phosphorylation.
Biophysical Chemistry, 2024, 305, 107155. DOI: 10.1016/j.bpc.2023.107155 HAL: hal-04440623v1

Melanie Rossotti, Diletta Arceri, Pascal Mansuelle, Olivier Bornet, Anne Durand, Soufian Ouchane, Hélène Launay, Pierre Dorlet.
The green cupredoxin CopI is a multicopper protein able to oxidize Cu(I).
Journal of Inorganic Biochemistry, 2024, 254, 112503. DOI: 10.1016/j.jinorgbio.2024.112503 HAL: hal-04448188v1

Maxime Bizet, Deborah Byrne, Frédéric Biaso, Guillaume Gerbaud, Emilien Etienne, Giuseppina Briola, Bruno Guigliarelli, Philippe Urban, Pierre Dorlet, Tamas Kalai, Gilles Truan, Marlene Martinho.
Structural insights into the semiquinone form of human Cytochrome P450 reductase by DEER distance measurements between a native flavin and a spin labelled non‐canonical amino acid.
Chemistry Eur J, 2024. DOI: 10.1002/chem.202304307 HAL: hal-04426634

Magali Roger, Philippe Leone, Ninian J. Blackburn, Sam Horrell, Tadeo Moreno Chicano, Frédéric Biaso, Marie-Thérèse Giudici-Orticoni, Luciano A. Abriata, Greg L. Hura, Michael A. Hough, Giuliano Sciara, Marianne Ilbert.
Beyond the coupled distortion model: structural analysis of the single domain cupredoxin AcoP, a green mononuclear copper centre with original features.
Dalton Trans., 2024, – . DOI: 10.1039/d3dt03372d HAL: hal-04386843v1

Marlène Martinho, Diane Allegro, Emilien Etienne, Cynthia Lohberger, Alessio Bonucci, Valérie Belle, Pascale Barbier.
Structural Flexibility of Tau in Its Interaction with Microtubules as Viewed by Site-Directed Spin Labeling EPR Spectroscopy.
Methods in Molecular Biology, 2024, 2754, 55 – 75. DOI: 10.1007/978-1-0716-3629-9_3

Xavier Kubiak, Ivan Polsinelli, Leonard M. G. Chavas, Cameron D. Fyfe, Alain Guillot, Laura Fradale, Clémence Brewee, Stéphane Grimaldi, Guillaume Gerbaud, Aurélien Thureau, Pierre Legrand, Olivier Berteau, Alhosna Benjdia.
Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme.
Nat Chem Biol, 2024. DOI: 10.1038/s41589-023-01493-1 HAL: hal-04371487

Margot Di Cesare, Elise Kaplan, Julia Rendon, Guillaume Gerbaud, Sepideh Valimehr, Alexia Gobet, Thu-Anh Thi Ngo, Vincent Chaptal, Pierre Falson, Marlène Martinho, Pierre Dorlet, Eric Hanssen, Jean-Michel Jault, Cédric Orelle.
The transport activity of the multidrug ABC transporter BmrA does not require a wide separation of the nucleotide-binding domains.
Journal of Biological Chemistry, 2024, 300 (1), 105546. DOI: 10.1016/j.jbc.2023.105546 HAL: hal-04435310

Ana Rita Oliveira, Cristiano Mota, Guilherme Vilela-Alves, Rita Rebelo Manuel, Neide Pedrosa, Vincent Fourmond, Kateryna Klymanska, Christophe Léger, Bruno Guigliarelli, Maria João Romão, Inês A. Cardoso Pereira.
An allosteric redox switch involved in oxygen protection in a CO2 reductase.
Nat Chem Biol, 2024, 20 (1), 111-119. DOI: 10.1038/s41589-023-01484-2 HAL: hal-04383526 (dataset available here)

Barbara Zambelli, Elisabetta Mileo.
Messages from cells: breaking new ground in deciphering protein behaviour.
Science Featured, 2024. (voir / see) HAL: hal-04512928


Duc-Duy Vu, Alessio Bonucci, Manon Brenière, Metztli Cisneros-Aguirre, Philippe Pelupessy, Ziqing Wang, Ludovic Carlier, Guillaume Bouvignies, Patricia Cortes, Aneel K. Aggarwal, Martin Blackledge, Zoher Gueroui, Valérie Belle, Jeremy M. Stark, Mauro Modesti, Fabien Ferrage.
Multivalent interactions of the disordered regions of XLF and XRCC4 foster robust cellular NHEJ and drive the formation of ligation-boosting condensates in vitro.
BioRxiv, 2023, . DOI: 10.1101/2023.07.12.548668 HAL: hal-04267167v1

Annalisa Pierro, Ketty Concetta Tamburrini, Hugo Leguenno, Guillaume Gerbaud, Emilien Etienne, Bruno Guigliarelli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR.
iScience, 2023, 26 (10), 107855. DOI: 10.1016/j.isci.2023.107855 HAL: hal-04233485v1

Guillaume Gerbaud, Brice Barbat, Mathilde Tribout, Emilien Etienne, Valérie Belle, Badreddine Douzi, Romé Voulhoux, Alessio Bonucci.
Refining the Dynamic Network of T2SS Endopilus Tip Heterocomplex Combining cw‐EPR and Nitroxide–Gd III Distance Measurements.
ChemBioChem, 2023, 24 (15), e2023000. DOI: 10.1002/cbic.202300099 HAL: hal-04210232v1

Pierre Nabokoff, Guillaume Brulay, Cyrielle Dol, Guillaume Gerbaud, Bruno Guigliarelli, Emilien Etienne, Emily Bloch, Fabio Ziarelli, Eric Besson, Stéphane Gastaldi.
Synthesis and Characterization of Face-to-Face Arylsulfanyl and Phenoxyl Radicals Embedded in Mesoporous Silicas.
J. Phys. Chem. C, 2023, 127 (20), 9699 – 9706. DOI: 10.1021/acs.jpcc.3c01550 HAL: hal-04114864v1

D. Marin, G. Gerbaud, O. Margeat, F. Ziarelli, F. Ferrer, O. Ouari, A. Campos, S. Bertaina, A. Savoyant.
Magnetic functionalization of ZnO nanoparticles surfaces via optically generated methyl radicals.
J. Chem. Phys., 2023, 158 (18). DOI: 10.1063/5.0152015 HAL: hal-04115234v1

Laura Prioretti, Giulia D’Ermo, Pascale Infossi, Arlette Kpebe, Régine Lebrun, Marielle Bauzan, Elisabeth Lojou, Bruno Guigliarelli, Marie-Thérèse Giudici-Orticoni, Marianne Guiral.
Carbon Fixation in the Chemolithoautotrophic Bacterium Aquifex aeolicus Involves Two Low-Potential Ferredoxins as Partners of the PFOR and OGOR Enzymes.
Life, 2023, 13 (3), 627. DOI: 10.3390/life13030627 HAL: hal-04071170v1

Emilien Etienne, Annalisa Pierro, Ketty C. Tamburrini, Alessio Bonucci, Elisabetta Mileo, Marlène Martinho, Valérie Belle.
Guidelines for the Simulations of Nitroxide X-Band cw EPR Spectra from Site-Directed Spin Labeling Experiments Using SimLabel.
Molecules, 2023, 28 (3), 1348. DOI: 10.3390/molecules28031348 HAL: hal-03971418v1

Alejandro Villalta, Batoul Srour, Audrey Lartigue, Martin Clémancey, Deborah Byrne, Florence Chaspoul, Antoine Loquet, Bruno Guigliarelli, Geneviève Blondin, Chantal Abergel, Bénédicte Burlat.
Evidence for [2Fe-2S] 2+ and Linear [3Fe-4S] 1+ Clusters in a Unique Family of Glycine/Cysteine-Rich Fe-S Proteins from Megavirinae Giant Viruses.
J. Am. Chem. Soc., 2023, 145 (5), 2733–2738. DOI: 10.1021/jacs.2c10484 HAL: hal-03967087v1


Annalisa Pierro, Alessio Bonucci, Davide Normanno, Mireille Ansaldi, Eric Pilet, Olivier Ouari, Bruno Guigliarelli, Emilien Etienne, Guillaume Gerbaud, Axel Magalon, Valérie Belle, Elisabetta Mileo.
Probing Structural Dynamics of a Bacterial Chaperone in Its Native Environment by Nitroxide‐Based EPR Spectroscopy.
Chemistry A European J, 2022. DOI: 10.1002/chem.202202249 HAL: hal-03836431

Batoul Srour, Sylvain Gervason, Maren Hellen Hoock, Beata Monfort, Kristian Want, Djabir Larkem, Nadine Trabelsi, Gautier Landrot, Andrea Zitolo, Emiliano Fonda, Emilien Etienne, Guillaume Gerbaud, Christina Sophia Müller, Jonathan Oltmanns, Jesse B. Gordon, Vishal Yadav, Malgorzata Kleczewska, Marcin Jelen, Michel B. Toledano, Rafal Dutkiewicz, David P. Goldberg, Volker Schünemann, Bruno Guigliarelli, Bénédicte Burlat, Christina Sizun, Benoit D’Autréaux.
Iron Insertion at the Assembly Site of the ISCU Scaffold Protein Is a Conserved Process Initiating Fe–S Cluster Biosynthesis.
J. Am. Chem. Soc., 2022. DOI: 10.1021/jacs.2c06338 HAL: hal-03837292v1

Maryam Seif-Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
Comparative analysis of the mena- and demethylmena-(semi)quinone binding mode to the quinol oxidation site of E. coli nitrate reductase A.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, 148750. DOI: 10.1016/j.bbabio.2022.148750

Julia Rendon, Margot Di Cesare, Alexia Godet, Guillaume Gerbaud, Emilien Etienne, Vincent Chaptal, Pierre Falson, Jean-Michel Jault, Cédric Orelle, Marlène Martinho, Pierre Dorlet.
Conformational dynamics of the ABC-transporter BmrA reveals by SDSL-EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863. DOI: 10.1016/j.bbabio.2022.148732

Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle.
EPR-MRS site: EPR spectroscopy for the study of biomolecules.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, Supplement, 29. DOI: 10.1016/j.bbabio.2022.148685 HAL: hal-03805551v1

Arlette Kpebe, Stéphane Grimaldi, Myriam Brugna, Eric Pilet.
First steps in the characterization of cytochrome bd oxidase from Solidesulfovibrio fructosovorans.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, 148691. DOI: 10.1016/j.bbabio.2022.148691

Pierre Dorlet, Guillaume Gerbaud, Emilien Etienne, Stéphane Grimaldi, Bruno Guigliarelli, Valérie Belle.
MOSBRI- A New European Research Infrastructure for Molecular Biophysics.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, Supplement, 28. DOI: 10.1016/j.bbabio.2022.148684 HAL: hal-03805560v1

Eric Pilet, Maryam Seif-Eddine, Farida Seduk, Julia Rendon, Bruno Guigliarelli, Stéphane Grimaldi, Axel Magalon.
New insights in quinone reactivity in the nitrate reductase A from E. coli.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2022, 1863, 148814. DOI: 10.1016/j.bbabio.2022.148814

Ana Rita Oliveira, Cristiano Mota, Kateryna Klymanska, Frédéric Biaso, Maria João Romão, Bruno Guigliarelli, Inês Cardoso Pereira.
Spectroscopic and Structural Characterization of Reduced Desulfovibrio vulgaris Hildenborough W-FdhAB Reveals Stable Metal Coordination during Catalysis.
ACS Chem. Biol., 2022, 17 (7), 1901 – 1909. DOI: 10.1021/acschembio.2c00336

Sofia M. Kapetanaki, Zsuzsanna Fekete, Pierre Dorlet, Marten H. Vos, Ursula Liebl, Andras Lukacs.
Molecular insights into the role of heme in the transcriptional regulatory system AppA/PpsR.
Biophysical Journal, 2022. DOI: 10.1016/j.bpj.2022.04.031 HAL: hal-03658174v1

Cameron D. Fyfe, Noelia Bernardo-García, Laura Fradale, Stéphane Grimaldi, Alain Guillot, Clémence Brewee, Leonard M. G. Chavas, Pierre Legrand, Alhosna Benjdia, Olivier Berteau.
Crystallographic snapshots of a B12-dependent radical SAM methyltransferase.
Nature, 2022. DOI: 10.1038/s41586-021-04355-9 HAL: hal-03808863v1

Eric Besson, Aurelien Vebr, Fabio Ziarelli, Emily Bloch, Guillaume Gerbaud, Séverine Queyroy, Pierre Thureau, Stéphane Viel, Stéphane Gastaldi.
Investigating the efficiency of silica materials with wall-embedded nitroxide radicals for dynamic nuclear polarisation NMR.
Phys. Chem. Chem. Phys., 2022, 24 (41), 25279 – 25286. DOI: 10.1039/d2cp02872g HAL: hal-03844800

Miguel Á. Casado-Combreras, Francisco Rivero-Rodríguez, Carlos A. Elena-Real, Dmitry Molodenskiy, Antonio Díaz-Quintana, Marlène Martinho, Guillaume Gerbaud, Katiuska González-Arzola, Adrián Velázquez-Campoy, Dmitri Svergun, Valérie Belle, Miguel A. De la Rosa, Irene Díaz-Moreno.
PP2A is activated by cytochrome c upon formation of a diffuse encounter complex with SET/TAF-Iβ.
Computational and Structural Biotechnology Journal, 2022, 20, 3695 – 3707. DOI: 10.1016/j.csbj.2022.07.009

Jérôme Gout, Floriane Meuris, Alain Desbois, Pierre Dorlet.
In vitro coordination of Fe-protoheme with amyloid β is non-specific and exhibits multiple equilibria.
Journal of Inorganic Biochemistry, 2022, 227, 111664. DOI: 10.1016/j.jinorgbio.2021.111664 HAL: hal-03460461


Annalisa Pierro, Alessio Bonucci, Valérie Belle, Elisabetta Mileo.
La RPE in cellula : étudier la dynamique des protéines dans leur habitat.
Actualité Chimique, 2021, 468, 38-41. (voir / see) HAL: hal-03855076v1

Anne Durand, Mélanie Fouesnard, Marie-Line Bourbon, Anne-Soisig Steunou, Elisabeth Lojou, Pierre Dorlet, Soufian Ouchane.
A periplasmic cupredoxin with a green CuT1.5 center is involved in bacterial copper tolerance.
Metallomics, 2021, 13 (12), mfab067. DOI: 10.1093/mtomcs/mfab067 HAL: hal-03453712v1

Sinan Al-Attar, Julia Rendon, Marlon Sidore, Jean-Pierre Duneau, Farida Seduk, Frédéric Biaso, Stéphane Grimaldi, Bruno Guigliarelli, Axel Magalon.
Gating of Substrate Access and Long-Range Proton Transfer in Escherichia coli Nitrate Reductase A: The Essential Role of a Remote Glutamate Residue.
ACS Catal., 2021, 14303 – 14318. DOI: 10.1021/acscatal.1c03988 HAL: hal-03511729v1

Rodrigo Arias-Cartin, Alexandre Uzel, Farida Seduk, Guillaume Gerbaud, Fabien Pierrel, Marianne Broc, Régine Lebrun, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi, Anne Walburger.
Identification and characterization of a non-canonical menaquinone-linked formate dehydrogenase.
Journal of Biological Chemistry, 2021, 101384. DOI: 10.1016/j.jbc.2021.101384 HAL: hal-03431446v1

Christelle Caux, Bruno Guigliarelli, Corinne Vivès, Frédéric Biaso, Marius Horeau, Hawra Hassoune, Isabelle Petit-Hartlein, Céline Juillan-Binard, Stephane Torelli, Franck Fieschi, Vincent Nivière.
Membrane-Bound Flavocytochrome MsrQ Is a Substrate of the Flavin Reductase Fre in Escherichia coli.
ACS Chem. Biol., 2021. DOI: 10.1021/acschembio.1c00613 HAL: hal-03320876v1

F. Torricella, A. Pierro, E. Mileo, V. Belle, A. Bonucci.
Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies.
Biochimica et Biophysica Acta (BBA) – Proteins and Proteomics, 2021, 1869 (7), 140653. DOI: 10.1016/j.bbapap.2021.140653 HAL: hal-03441104v1

Cheryle N. Beuning, Luca J. Zocchi, Kyangwi P. Malikidogo, Charlène Esmieu, Pierre Dorlet, Debbie C. Crans, Christelle Hureau.
Measurement of Interpeptidic Cu(II) Exchange Rate Constants of Cu(II)-Amyloid-β Complexes to Small Peptide Motifs by Tryptophan Fluorescence Quenching.
Inorg. Chem., 2021, 60, 7650-7659. DOI: 10.1021/acs.inorgchem.0c03555 HAL: hal-03380483

Umberto Contaldo, Bruno Guigliarelli, Julien Perard, Clara Rinaldi, Alan Le Goff, Christine Cavazza.
Efficient Electrochemical CO2/CO Interconversion by an Engineered Carbon Monoxide Dehydrogenase on a Gas-Diffusion Carbon Nanotube-Based Bioelectrode.
ACS Catal., 2021, 11 (9), 5808 – 5817. DOI: 10.1021/acscatal.0c05437 HAL: hal-03258521v1

Majid Haddad Momeni, Folmer Fredslund, Bastien Bissaro, Olanrewaju Raji, Thu V. Vuong, Sebastian Meier, Tine Sofie Nielsen, Vincent Lombard, Bruno Guigliarelli, Frédéric Biaso, Mireille Haon, Sacha Grisel, Bernard Henrissat, Ditte Hededam Welner, Emma R. Master, Jean-Guy Berrin, Maher Abou Hachem.
Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs.
Nat Commun, 2021, 12 (1). DOI: 10.1038/s41467-021-22372-0 HAL: hal-03234953v1

Anouchka Fiévet, Meriem Merrouch, Gaël Brasseur, Danaé Eve, Emanuele G. Biondi, Odile Valette, Sofia R. Pauleta, Alain Dolla, Zorah Dermoun, Bénédicte Burlat, Corinne Aubert.
OrpR is a σ 54 ‐dependent activator using an iron‐sulfur cluster for redox sensing in Desulfovibrio vulgaris Hildenborough.
Mol. Microbiol., 2021, 116 (1), 231 – 244. DOI: 10.1111/mmi.14705 HAL: hal-03204123v1

Romain Clément, Xie Wang, Frédéric Biaso, Marianne Ilbert, Ievgen Mazurenko, Elisabeth Lojou.
Mutations in the coordination spheres of T1 Cu affect Cu2+-activation of the laccase from Thermus thermophilus.
Biochimie, 2021, 182, 228-237. DOI: 10.1016/j.biochi.2021.01.006 HAL: hal-03127150v1

Karl J. Koebke, Toni Kühl, Elisabeth Lojou, Borries Demeler, Barbara Schoepp-Cothenet, Olga Iranzo, Vincent L. Pecoraro, Anabella Ivancich.
The pH‐Induced Selectivity Between Cysteine or Histidine Coordinated Heme in an Artificial α‐Helical Metalloprotein.
Angew. Chem. Int. Ed., 2021, 60 (8), 3974 – 3978. DOI: 10.1002/anie.202012673 HAL: hal-03017501


Valérie Belle, André Fournel.
Using paramagnetic probes to study structural transitions in proteins.
Electron Paramagnetic Resonance Spectroscopy – Applications, 2020, 219-246. DOI: 10.1007/978-3-030-39668-8_8

Mark J Burton, Joel Cresser-Brown, Morgan Thomas, Nicola Portolano, Jaswir Basran, Samuel L Freeman, Hanna Kwon, Andrew R. Bottrill, Manuel J Llansola-Portoles, Andrew A Pascal, Rebekah Jukes-Jones, Tatyana Chernova, Ralf Schmid, Noel W Davies, Nina M Storey, Pierre Dorlet, Peter C. E. Moody, John S Mitcheson, Emma L Raven.
Discovery of a heme-binding domain in a neuronal voltage-gated potassium channel.
J. Biol. Chem., 2020, 295 (38), 13277 – 13286. DOI: 10.1074/jbc.RA120.014150 HAL: hal-02928173v1

Ahmed Djeghader, Mélanie Rossotti, Saleh Abdulkarim, Frédéric Biaso, Guillaume Gerbaud, Wolfgang Nitschke, Barbara Schoepp-Cothenet, Tewfik Soulimane, Stephane Grimaldi.
Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase.
Chem. Commun., 2020, 56 (68), 9850 – 9853. DOI: 10.1039/D0CC03634J HAL: hal-02930401

Annalisa Pierro, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Stefano Ciurli, Valérie Belle, Barbara Zambelli, Elisabetta Mileo.
Nickel and GTP Modulate Helicobacter pylori UreG Structural Flexibility.
Biomolecules, 2020, 10 (7), 1062. DOI: 10.3390/biom10071062 HAL: hal-02902916v1

Maryam Seif Eddine, Frédéric Biaso, Julia Rendon, Eric Pilet, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
1,2H hyperfine spectroscopy and DFT modeling unveil the demethylmenasemiquinone binding mode to E. coli nitrate reductase A (NarGHI).
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2020, 1861 (8), 148203. DOI: 10.1016/j.bbabio.2020.148203 HAL: hal-02909846

Ana Rita Oliveira, Cristiano Mota, Cláudia Mourato, Renato M. Domingos, Marino F. A. Santos, Diana Gesto, Bruno Guigliarelli, Teresa Santos-Silva, Maria João Romão, Inês A. Cardoso Pereira.
Toward the Mechanistic Understanding of Enzymatic CO2 Reduction.
ACS Catal., 2020, 10 (6), 3844 – 3856. DOI: 10.1021/acscatal.0c00086 HAL: hal-02938569

Sarmistha Bhunia, Atanu Rana, Somdatta Ghosh Dey, Anabella Ivancich, Abhishek Dey.
A designed second-sphere hydrogen-bond interaction that critically influences the O–O bond activation for heterolytic cleavage in ferric iron–porphyrin complexes.
Chem. Sci., 2020, 11 (10), 2681 – 2695. DOI: 10.1039/C9SC04388H HAL: hal-02887080v1

Alessio Bonucci, Olivier Ouari, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo.
In‐Cell EPR: Progress towards Structural Studies Inside Cells.
ChemBioChem, 2020, 21 (4), 451 – 460. DOI: 10.1002/cbic.201900291 HAL: hal-02180300v1

Pierre Dorlet, Christelle Hureau.
Copper complexation by peptides implicated in neurodegenerative diseases.
Electron Paramagnetic Resonance Spectroscopy – Applications, 2020, 83-108. DOI: 10.1007/978-3-030-39668-8_4


Eugénie Fournier, Sybille Tachon, Nicholas J. Fowler, Guillaume Gerbaud, Pascal Mansuelle, Pierre Dorlet, Sam P. De Visser, Valérie Belle, A. Jalila Simaan, Marlène Martinho.
The Hunt for the Closed Conformation of the Fruit‐Ripening Enzyme 1‐Aminocyclopropane‐1‐carboxylic Oxidase: A Combined Electron Paramagnetic Resonance and Molecular Dynamics Study.
Chem. Eur. J., 2019, 25 (60), 13766-13776. DOI: 10.1002/chem.201903003 HAL: hal-02303326v1

Pascale Barbier, Orgeta Zejneli, Marlène Martinho, Alessia Lasorsa, Valérie Belle, Caroline Smet-Nocca, Philipp O. Tsvetkov, François Devred, Isabelle Landrieu.
Role of Tau as a Microtubule-Associated Protein: Structural and Functional Aspects.
Front. Aging Neurosci., 2019, 11:204, 1 – 14. DOI: 10.3389/fnagi.2019.00204 HAL: hal-02279660v1

Sybille Tachon, Eugénie Fournier, Christophe Decroos, Pascal Mansuelle, Emilien Etienne, Marc Maresca, Marlène Martinho, Valérie Belle, Thierry Tron, Ariane Jalila Simaan.
Chemical Modification of 1-Aminocyclopropane Carboxylic Acid (ACC) Oxidase: Cysteine Mutational Analysis, Characterization, and Bioconjugation with a Nitroxide Spin Label.
Mol Biotechnol, 2019, 61, 650 – 662. DOI: 10.1007/s12033-019-00191-5 HAL: hal-02157289v1

Kamal Zeamari, Guillaume Gerbaud, Sandrine Grosse, Vincent Fourmond, Florence Chaspoul, Frédéric Biaso, Pascal Arnoux, Monique Sabaty, David Pignol, Bruno Guigliarelli, Bénédicte Burlat.
Tuning the redox properties of a [4Fe-4S] center to modulate the activity of Mo-bisPGD periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2019, 1860 (5), 402 – 413. DOI: 10.1016/j.bbabio.2019.01.003 HAL: hal-02106668v1

Cyrielle Dol, Guillaume Gerbaud, Bruno Guigliarelli, Emily Bloch, Stéphane Gastaldi, Eric Besson.
Modulating lifetimes and relaxation times of phenoxyl radicals through their incorporation into different hybrid nanostructures.
Phys. Chem. Chem. Phys., 2019, 172 (29), 573 – 16344. DOI: 10.1039/C9CP03052B HAL: hal-02177931v1

Nolwenn Le Breton, Sonia Longhi, Antal Rockenbauer, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle, Marlène Martinho.
Probing the dynamic properties of two sites simultaneously in a protein–protein interaction process: a SDSL-EPR study.
Phys. Chem. Chem. Phys., 2019, 21, 22584 – 22588. DOI: 10.1039/c9cp04660g HAL: hal-02341622v1

Marlène Martinho, Eugénie Fournier, Nolwenn Le Breton, Elisabetta Mileo, Valérie Belle.
Nitroxide spin labels: fabulous spy spins for biostructural EPR applications.
Electron Paramagnetic Resonance, 2019, 26, 66 – 88. DOI: 10.1039/9781788013888-00066 HAL: hal-01953977v1

Eugénie Fournier, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Valérie Belle, Marlène Martinho.
Spin labels: spies in the heart of proteins.
Actualité Chimique, 2019, 443. (voir / see)


Arlette Kpebe, Martino Benvenuti, Chloé Guendon, Amani Rebai, Victoria Fernandez, Sébastien Le Laz, Emilien Etienne, Bruno Guigliarelli, Gabriel García-Molina, Antonio L. de Lacey, Carole Baffert, Myriam Brugna.
A new mechanistic model for an O2-protected electron-bifurcating hydrogenase, Hnd from Desulfovibrio fructosovorans.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (12), 1302 – 1312. DOI: 10.1016/j.bbabio.2018.09.364 HAL: hal-01928576v1

Marlène Martinho, Diane Allegro, Isabelle Huvent, Charlotte Chabaud, Emilien Etienne, Hervé Kovacic, Bruno Guigliarelli, Vincent Peyrot, Isabelle Landrieu, Valérie Belle, Pascale Barbier.
Two Tau binding sites on tubulin revealed by thiol-disulfide exchanges.
Sci Rep, 2018, 8:13846, 1 – 11. DOI: 10.1038/s41598-018-32096-9 HAL: hal-01883844v1

Angeliki M. Andrianaki, Irene Kyrmizi, Kalliopi Thanopoulou, Clara Baldin, Elias Drakos, Sameh S. M. Soliman, Amol C. Shetty, Carrie McCracken, Tonia Akoumianaki, Kostas Stylianou, Petros Ioannou, Charalampos Pontikoglou, Helen A. Papadaki, Maria Tzardi, Valerie Belle, Emilien Etienne, Anne Beauvais, George Samonis, Dimitrios P. Kontoyiannis, Evangelos Andreakos, Vincent M. Bruno, Ashraf S. Ibrahim, Georgios Chamilos.
Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species.
Nat Commun, 2018, 11 (Suppl. 1), R106. DOI: 10.1038/s41467-018-05820-2 HAL: hal-01892640v1

E Mileo, M Ilbert, A Barducci, P Bordes, M-P Castanié-Cornet, C Garnier, P Genevaux, R Gillet, P Goloubinoff, F Ochsenbein, G Richarme, C Iobbi-Nivol, M-T Giudici, B Gontero , O Genest.
Emerging fields in chaperone proteins: A French workshop.
Biochimie, 2018, 151, 159 – 165. DOI: 10.1016/j.biochi.2018.06.004 HAL: hal-01863220v1

Pascal Griesmar, Marlène Martinho, Bruno Faure.
Synthèse de complexes du vanadium et leur analyse par spectroscopie d’absorption UV–visible et infra-rouge.
Bulletin de l’Union des Physiciens, 01/06/2018, 2018, 6, 1005.

Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, David Guieysse, Laurence Girbal, Vincent Fourmond, Isabelle André, Bruno Guigliarelli, Christophe Léger, Philippe Soucaille, Isabelle Meynial-Salles.
Roles of the F-domain in [FeFe] hydrogenase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2018, 1859 (2), 69 – 77. DOI: 10.1016/j.bbabio.2017.08.010 HAL: cea-01950818

Ganesan Karthikeyan, Alessio Bonucci, Gilles Casano, Guillaume Gerbaud, Sébastien Abel, Virginie Thomé, Laurent Kodjabachian, Axel Magalon, Bruno Guigliarelli, Valérie Belle, Olivier Ouari, Elisabetta Mileo.
A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies.
Angew. Chem. Int. Ed., 2018, 57, 1366 – 1370. DOI: 10.1002/anie.201710184 HAL: hal-02000559v1

Xie Wang, Magali Roger, Romain Clément, Sophie Lecomte, Frédéric Biaso, Luciano A. Abriata, Pascal Mansuelle, Ievgen Mazurenko, Marie-Thérèse Giudici-Orticoni, Elisabeth Lojou, Marianne Ilbert.
Electron transfer in an acidophilic bacterium: interaction between a diheme cytochrome and a cupredoxin.
Chem. Sci., 2018, 6 (21), 4879 – 4891. DOI: 10.1039/C8SC01615A HAL: hal-01793582v1

Anabella Ivancich, Peter C. Loewen.
Electron Transfer in Catalases and Catalase-Peroxidases.
Encyclopedia of Biophysics, European Biophysical Societies’ Association (EBSA), (Roberts G., Watts A., Eds). Springer, Berlin, Heidelberg., 2018. DOI: 10.1007/978-3-642-35943-9_51-1 HAL: hal-01798212v1


Christoph Gmeiner, Daniel Klose, Elisabetta Mileo, Valérie Belle, Sylvain R. A. Marque, Georg Dorn, Frédéric H. T. Allain, Bruno Guigliarelli, Gunnar Jeschke, Maxim Yulikov.
Orthogonal Tyrosine and Cysteine Site-Directed Spin Labeling for Dipolar Pulse EPR Spectroscopy on Proteins.
J. Phys. Chem. Lett., 2017, 8 (19), 4852 – 4857. DOI: 10.1021/acs.jpclett.7b02220 HAL: hal-01596569v1

Maryam Seif Eddine, Frédéric Biaso, Rodrigo Arias‐Cartin, Eric Pilet, Julia Rendon, Sevdalina Lyubenova, Farida Seduk, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi.
Probing the Menasemiquinone Binding Mode to Nitrate Reductase A by Selective 2 H and 15 N Labeling, HYSCORE Spectroscopy, and DFT Modeling.
ChemPhysChem, 2017, 18 (19), 2704 – 2714. DOI: 10.1002/cphc.201700571 HAL: hal-01635739v1

Marta Palombo, Alessio Bonucci, Emilien Etienne, Stefano Ciurli, Vladimir N. Uversky, Bruno Guigliarelli, Valérie Belle, Elisabetta Mileo, Barbara Zambelli.
The relationship between folding and activity in UreG, an intrinsically disordered enzyme.
Sci Rep, 2017, 89 (1), 392. DOI: 10.1038/s41598-017-06330-9 HAL: hal-01585289v1

Nadia El Bakkali-Tahéri, Sybille Tachon, Maylis Orio, Sylvain Bertaina, Marlène Martinho, Viviane Robert, Marius Réglier, Thierry Tron, Pierre Dorlet, A. Jalila Simaan.
Characterization of Cu(II)-reconstituted ACC Oxidase using experimental and theoretical approaches.
Archives of Biochemistry and Biophysics, 2017, 623-624, 31-41. DOI: 10.1016/ HAL: hal-01627933v1

Magali Roger, Giuliano Sciara, Frédéric Biaso, Elisabeth Lojou, Xie Wang, Marielle Bauzan, Marie-Thérèse Giudici-Orticoni, Alejandro J. Vila, Marianne Ilbert.
Impact of copper ligand mutations on a cupredoxin with a green copper center.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2017, 1858 (5), 351 – 359. DOI: 10.1016/j.bbabio.2017.02.007 HAL: hal-01481398v1

Julia Rendon, Frédéric Biaso, Pierre Ceccaldi, René Toci, Farida Seduk, Axel Magalon, Bruno Guigliarelli, Stéphane Grimaldi.
Elucidating the Structures of the Low- and High-pH Mo(V) Species in Respiratory Nitrate Reductase: A Combined EPR, 14,15 N HYSCORE, and DFT Study.
Inorg. Chem., 2017, 56 (8), 4422 – 4434. DOI: 10.1021/acs.inorgchem.6b03129 HAL: hal-01514650v1

Jennifer O. Bigelow, Jason England, Johannes E. M. N. Klein, Erik R. Farquhar, Jonathan R. Frisch, Marlène Martinho, Debasish Mandal, Eckard Münck, Sason Shaik, Lawrence Que.
Oxoiron(IV) Tetramethylcyclam Complexes with Axial Carboxylate Ligands: Effect of Tethering the Carboxylate on Reactivity.
Inorg. Chem., 2017, 56 (6), 3287 – 3301. DOI: 10.1021/acs.inorgchem.6b02659 HAL: hal-01514906v1

Emilien Etienne, Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Valérie Belle.
SimLabel: a graphical user interface to simulate continuous wave EPR spectra from site-directed spin labeling experiments.
Magn. Reson. Chem., 2017, 55 (8), 714 – 719. DOI: 10.1002/mrc.4578 HAL: hal-01444172v1

Alda Lisa Concia, Maria Rosa Beccia, Maylis Orio, Francine Terra Ferre, Marciela Scarpellini, Frédéric Biaso, Bruno Guigliarelli, Marius Réglier, A. Jalila Simaan.
Copper Complexes as Bioinspired Models for Lytic Polysaccharide Monooxygenases.
Inorg. Chem., 2017, 56 (3), 1023 – 1026. DOI: 10.1021/acs.inorgchem.6b02165 HAL: hal-01440965v1


Rodrigo Arias-Cartin, Pierre Ceccaldi, Barbara Schoepp-Cothenet, Klaudia Frick, Jean-Michel Blanc, Bruno Guigliarelli, Anne Walburger, Stéphane Grimaldi, Thorsten Friedrich, Véronique Receveur-Brechot, Axel Magalon.
Redox cofactors insertion in prokaryotic molybdoenzymes occurs via a conserved folding mechanism.
Sci Rep, 2016, 12 (1), 543. DOI: 10.1038/srep37743 HAL: hal-01415067v1

Simon Duval, Joanne M. Santini, David Lemaire, Florence Chaspoul, Michael J. Russell, Stephane Grimaldi, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
The H-bond network surrounding the pyranopterins modulates redox cooperativity in the molybdenum- bis PGD cofactor in arsenite oxidase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2016, 1857 (9), 1353 – 1362. DOI: 10.1016/j.bbabio.2016.05.003 HAL: hal-01413292v1

Sona Garajova, Yann Mathieu, Maria Rosa Beccia, Chloé Bennati-Granier, Frédéric Biaso, Mathieu Fanuel, David Ropartz, Bruno Guigliarelli, Eric Record, Hélène Rogniaux, Bernard Henrissat, Jean-Guy Berrin.
Single-domain flavoenzymes trigger lytic polysaccharide monooxygenases for oxidative degradation of cellulose.
Sci Rep, 2016, 274 (1), 3338. DOI: 10.1038/srep28276 HAL: hal-01413367v1

Pierre Ceccaldi, Emilien Etienne, Sébastien Dementin, Bruno Guigliarelli, Christophe Léger, Bénédicte Burlat.
Mechanism of inhibition of NiFe hydrogenase by nitric oxide.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2016, 1857 (4), 454 – 461. DOI: 10.1016/j.bbabio.2016.01.014 HAL: hal-01413192v1

Yannick H. Ouellet, Cheikh Tidiane Ndiaye, Stéphane M. Gagné, Anne Sebilo, Michael D.L. Suits, Éric Jubinville, Zongchao Jia, Anabella Ivancich, Manon Couture.
An alternative reaction for heme degradation catalyzed by the Escherichia coli O157:H7 ChuS protein: Release of hematinic acid, tripyrrole and Fe(III).
Journal of Inorganic Biochemistry, 2016, 154, 103 – 113. DOI: 10.1016/j.jinorgbio.2015.11.002 HAL: hal-01406050v1

Nolwenn Le Breton, Tiona Adrianaivomananjaona, Guillaume Gerbaud, Emilien Etienne, Elena Bisetto, Alain Dautant, Bruno Guigliarelli, Francis Haraux, Marlène Martinho, Valérie Belle.
Dimerization interface and dynamic properties of yeast IF1 revealed by Site-Directed Spin Labeling EPR spectroscopy.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2016, 1857 (1), 89 – 97. DOI: 10.1016/j.bbabio.2015.10.010 HAL: hal-01413333v1

Stéphane Grimaldi, Frédéric Biaso, Bénédicte Burlat, Bruno Guigliarelli.
Electron paramagnetic resonance studies of molybdenum enzymes.
Molybdenum and Tungsten Enzymes : Spectroscopic and Theoretical Investigations. RSC Metallobiology Series. Editors: Russ Hille, Carola Schulzke, Martin L Kirk, 2016, 68 – 120. DOI: 10.1039/9781782628842-00068 HAL: hal-01441142v1

Eric Besson, Fabio Ziarelli, Emily Bloch, Guillaume Gerbaud, Séverine Queyroy, Stéphane Viel, Stéphane Gastaldi.
Silica materials with wall-embedded nitroxides provide efficient polarization matrices for dynamic nuclear polarization NMR.
Chem. Commun., 2016, 52 (32), 5531 – 5533. DOI: 10.1039/c6cc01809b HAL: hal-01415149v1


Pierre Ceccaldi, Julia Rendon, Christophe Léger, René Toci, Bruno Guigliarelli, Axel Magalon, Stéphane Grimaldi, Vincent Fourmond.
Reductive activation of E. coli respiratory nitrate reductase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2015, 1847 (10), 1055 – 1063. DOI: 10.1016/j.bbabio.2015.06.007 HAL: hal-01429839v1

Linda Payet, Marlène Martinho, Céline Merstorf, Manuela Pastoriza-Gallego, Juan Pelta, Virgile Viasnoff, Loïc Auvray, Murugappan Muthukumar, Jérôme Mathé.
Temperature Effect on Ionic Current and ssDNA Transport through Nanopores.
Biophysical Journal, 2015, 109 (8), 1600 – 1607. DOI: 10.1016/j.bpj.2015.08.043 HAL: hal-01444203v1

Jeremy Guérin, Nathalie Saint, Catherine Baud, Albano C. Meli, Emilien Etienne, Camille Locht, Hervé Vezin, Françoise Jacob-Dubuisson.
Dynamic interplay of membrane-proximal POTRA domain and conserved loop L6 in Omp85 transporter FhaC.
Molecular Microbiology, 2015, 98 (3), 490 – 501. DOI: 10.1111/mmi.13137 HAL: hal-01432130v1

Julia Rendon, Eric Pilet, Zeinab Fahs, Farida Seduk, Léa Sylvi, Mahmoud Hajj Chehade, Fabien Pierrel, Bruno Guigliarelli, Axel Magalon, Stephane Grimaldi.
Demethylmenaquinol is a substrate of Escherichia coli nitrate reductase A (NarGHI) and forms a stable semiquinone intermediate at the NarGHI quinol oxidation site.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2015, 1847 (8), 739 – 747. DOI: 10.1016/j.bbabio.2015.05.001 HAL: hal-01429032v1

Nolwenn Le Breton, Marlène Martinho, Elisabetta Mileo, Emilien Etienne, Guillaume Gerbaud, Bruno Guigliarelli, Valérie Belle.
Exploring intrinsically disordered proteins using site-directed spin labeling electron paramagnetic resonance spectroscopy.
Front. Mol. Biosci., 2015, 2:21, 1 – 7. DOI: 10.3389/fmolb.2015.00021 HAL: hal-01429982v1

Peter C. Loewen, Jacylyn Villanueva, Jacek Switala, Lynda J. Donald, Anabella Ivancich.
Unprecedented access of phenolic substrates to the heme active site of a catalase: Substrate binding and peroxidase-like reactivity of Bacillus pumilus catalase monitored by X-ray crystallography and EPR spectroscopy.
Proteins, 2015, 83 (5), 853 – 866. DOI: 10.1002/prot.24777 HAL: hal-01432180v1

Colin W. J. Lockwood, Bénédicte Burlat, Myles R. Cheesman, Melanie Kern, Jörg Simon, Thomas A. Clarke, David J. Richardson, Julea N. Butt.
Resolution of Key Roles for the Distal Pocket Histidine in Cytochrome c Nitrite Reductases.
J. Am. Chem. Soc., 2015, 137 (8), 3059 – 3068. DOI: 10.1021/ja512941j HAL: hal-01435358v1

Abbas Abou-Hamdan, Pierre Ceccaldi, Hugo Lebrette, Oscar Gutiérrez-Sanz, Pierre Richaud, Laurent Cournac, Bruno Guigliarelli, Antonio L. De Lacey, Christophe Léger, Anne Volbeda, Bénédicte Burlat, Sébastien Dementin.
A Threonine Stabilizes the NiC and NiR Catalytic Intermediates of [NiFe]-hydrogenase.
J. Biol. Chem., 2015, 290 (13), 8550 – 8558. DOI: 10.1074/jbc.M114.630491 HAL: hal-01149507v1

Arianna I. Celis, Zachary Geeraerts, David Ngmenterebo, Melodie M. Machovina, Richard C. Kurker, Kumar Rajakumar, Anabella Ivancich, Kenton R. Rodgers, Gudrun S. Lukat-Rodgers, Jennifer L. DuBois.
A Dimeric Chlorite Dismutase Exhibits O 2 -Generating Activity and Acts as a Chlorite Antioxidant in Klebsiella pneumoniae MGH 78578.
Biochemistry, 2015, 54 (2), 434 – 446. DOI: 10.1021/bi501184c HAL: hal-01432151v1


Audrey Lartigue, Bénédicte Burlat, Bruno Coutard, Florence Chaspoul, Jean-Michel Claverie, Chantal Abergel.
The Megavirus Chilensis Cu,Zn-Superoxide Dismutase: the First Viral Structure of a Typical Cellular Copper Chaperone-Independent Hyperstable Dimeric Enzyme.
J. Virol., 2014, 89 (1), 824 – 832. DOI: 10.1128/JVI.02588-14 HAL: hal-01432503v1

Julien G.J. Jacques, Bénédicte Burlat, Pascal Arnoux, Monique Sabaty, Bruno Guigliarelli, Christophe Léger, David Pignol, Vincent Fourmond.
Kinetics of substrate inhibition of periplasmic nitrate reductase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2014, 1837 (10), 1801 – 1809. DOI: 10.1016/j.bbabio.2014.05.357 HAL: hal-01494456v1

Magali Roger, Frédéric Biaso, Cindy J. Castelle, Marielle Bauzan, Florence Chaspoul, Elisabeth Lojou, Giuliano Sciara, Stefano Caffarri, Marie-Thérèse Giudici-Orticoni, Marianne Ilbert.
Spectroscopic Characterization of a Green Copper Site in a Single-Domain Cupredoxin.
PLoS ONE, 2014, 9 (6), 1 – 13. DOI: 10.1371/journal.pone.0098941 HAL: hal-01494439v1

Kyle D. Miner, Thomas D. Pfister, Parisa Hosseinzadeh, Nadime Karaduman, Lynda J. Donald, Peter C. Loewen, Yi Lu, Anabella Ivancich.
Identifying the Elusive Sites of Tyrosyl Radicals in Cytochrome c Peroxidase: Implications for Oxidation of Substrates Bound at a Site Remote from the Heme.
Biochemistry, 2014, 53 (23), 3781 – 3789. DOI: 10.1021/bi500353p HAL: hal-01556875v1

Julien G.J. Jacques, Vincent Fourmond, Pascal Arnoux, Monique Sabaty, Emilien Etienne, Sandrine Grosse, Frédéric Biaso, Patrick Bertrand, David Pignol, Christophe Léger, Bruno Guigliarelli, Bénédicte Burlat.
Reductive activation in periplasmic nitrate reductase involves chemical modifications of the Mo-cofactor beyond the first coordination sphere of the metal ion.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2014, 1837 (2), 277 – 286. DOI: 10.1016/j.bbabio.2013.10.013 HAL: hal-01481487v1

Jason England, Jennifer O. Bigelow, Katherine M. Van Heuvelen, Erik R. Farquhar, Marlène Martinho, Katlyn K. Meier, Jonathan R. Frisch, Eckard Münck, Lawrence Que.
An ultra-stable oxoiron(IV) complex and its blue conjugate base.
Chem. Sci., 2014, 5, 1204 – 1215. DOI: 10.1039/C3SC52755G

Nolwenn Le Breton, Marlène Martinho, Kuanysh Kabytaev, Jérémie Topin, Elisabetta Mileo, David Blocquel, Johnny Habchi, Sonia Longhi, Antal Rockenbauer, Jérôme Golebiowski, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Diversification of EPR signatures in site directed spin labeling using a β-phosphorylated nitroxide.
Phys. Chem. Chem. Phys., 2014, 16, 4202 – 4209. DOI: 10.1039/c3cp54816c HAL: hal-01460516v1


Stéphane Grimaldi, Barbara Schoepp-Cothenet, Pierre Ceccaldi, Bruno Guigliarelli, Axel Magalon.
The prokaryotic Mo/W-bisPGD enzymes family: A catalytic workhorse in bioenergetic.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2013, 1827 (8-9), 1048 – 1085. DOI: 10.1016/j.bbabio.2013.01.011 HAL: hal-03549630

Elisabetta Mileo, Emilien Etienne, Marlène Martinho, Régine Lebrun, Valérie Roubaud, Paul Tordo, Brigitte Gontero, Bruno Guigliarelli, Sylvain R. A. Marque, Valérie Belle.
Enlarging the Panoply of Site-Directed Spin Labeling Electron Paramagnetic Resonance (SDSL-EPR): Sensitive and Selective Spin-Labeling of Tyrosine Using an Isoindoline-Based Nitroxide.
Bioconjugate Chem., 2013, 24 (6), 1110 – 1117. DOI: 10.1021/bc4000542 HAL: hal-01460460v1

Monique Sabaty, Sandrine Grosse, Geraldine Adryanczyk, Séverine Boiry, Frédéric Biaso, Pascal Arnoux, David Pignol.
Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis.
BMC Biochemistry, 2013, 14 (article 28) (1). DOI: 10.1186/1471-2091-14-28

Elisabetta Mileo, Magali Lorenzi, Jenny Erales, Sabrina Lignon, Carine Puppo, Nolwenn Le Breton, Emilien Etienne, Sylvain R. A. Marque, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
Dynamics of the intrinsically disordered protein CP12 in its association with GAPDH in the green alga Chlamydomonas reinhardtii: a fuzzy complex.
Mol. BioSyst., 2013, 1 (11), 349. DOI: 10.1039/c3mb70190e HAL: hal-01460463v1

Abbas Abou Hamdan, Bénédicte Burlat, Oscar Gutiérrez-Sanz, Pierre-Pol Liebgott, Carole Baffert, Antonio L De Lacey, Marc Rousset, Bruno Guigliarelli, Christophe Léger, Sébastien Dementin.
O2-independent formation of the inactive states of NiFe hydrogenase.
Nat Chem Biol, 2013, 9, 15 – 17. DOI: 10.1038/NCHEMBIO.1110 HAL: hal-01556905v1


Rodrigo Arias-Cartin, Stéphane Grimaldi, Pascal Arnoux, Bruno Guigliarelli, Axel Magalon.
Cardiolipin binding in bacterial respiratory complexes: Structural and functional implications.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2012, 1817 (10), 1937 – 1949. DOI: 10.1016/j.bbabio.2012.04.005 HAL: hal-03549629v1

Marlène Martinho, Johnny Habchi, Zeina El Habre, Léo Nesme, Bruno Guigliarelli, Valérie Belle, Sonia Longhi.
Assessing induced folding within the intrinsically disordered C-terminal domain of the Henipavirus nucleoproteins by site-directed spin labeling EPR spectroscopy.
J. Biomol. Struct. Dyn., 2012, 31, 453-471. DOI: 10.1080/07391102.2012.706068

Linda Payet, Marlène Martinho, Manuela Pastoriza-Gallego, Jean-Michel Betton, Loïc Auvray, Juan Pelta, Jérôme Mathé.
Thermal Unfolding of Proteins Probed at the Single Molecule Level Using Nanopores.
Anal. Chem., 2012, 84 (9), 4071 – 4076. DOI: 10.1021/ac300129e

Frédéric Biaso, Bénédicte Burlat, Bruno Guigliarelli.
DFT Investigation of the Molybdenum Cofactor in Periplasmic Nitrate Reductases: Structure of the Mo(V) EPR-Active Species.
Inorg. Chem., 2012, 51 (6), 3409 – 3419. DOI: 10.1021/ic201533p

Johnny Habchi, Marlène Martinho, Antoine Gruet, Bruno Guigliarelli, Sonia Longhi, Valérie Belle.
Monitoring structural transitions in IDPs by site-directed spin-labeling EPR spectroscopy.
Intrinsically Disordered Protein Analysis Volume 1, Methods and Experimental Tools, 2012, 361-386. DOI: 10.1007/978-1-61779-927-3_21


Stéphane Grimaldi, Rodrigo Arias-Cartin, Pascal Lanciano, Sevdalina Lyubenova, Rodolphe Szenes, Burkhard Endeward, Thomas F. Prisner, Bruno Guigliarelli, Axel Magalon.
Determination of the Proton Environment of High Stability Menasemiquinone Intermediate in Escherichia coli Nitrate Reductase A by Pulsed EPR.
J. Biol. Chem., 2011, 287 (7), 4662 – 4670. DOI: 10.1074/jbc.M111.325100 HAL: hal-03248799v1

Frédéric Halgand, Johnny Habchi, Laetitia Cravello, Marlène Martinho, Bruno Guigliarelli, Sonia Longhi.
Dividing To Unveil Protein Microheterogeneities: Traveling Wave Ion Mobility Study.
Anal. Chem., 2011, 83 (19), 7306 – 7315. DOI: 10.1021/ac200994c HAL: hal-00677256v1

Magali Lorenzi, Carine Puppo, Régine Lebrun, Sabrina Lignon, Valérie Roubaud, Marlène Martinho, Elisabetta Mileo, Paul Tordo, Sylvain R. A. Marque, Brigitte Gontero, Bruno Guigliarelli, Valérie Belle.
Tyrosine-Targeted Spin Labeling and EPR Spectroscopy: An Alternative Strategy for Studying Structural Transitions in Proteins.
Angew. Chem. Int. Ed., 2011, 50, 9108 – 9111. DOI: 10.1002/anie.201102539 HAL: hal-00677247v1

Sébastien Dementin, Bénédicte Burlat, Vincent Fourmond, Fanny Leroux, Pierre-Pol Liebgott, Abbas Abou Hamdan, Christophe Léger, Marc Rousset, Bruno Guigliarelli, Patrick Bertrand.
Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements.
J. Am. Chem. Soc., 2011, 133 (26), 10211 – 10221. DOI: 10.1021/ja202615a HAL: hal-00677402v1

R. Arias-Cartin, S. Grimaldi, J. Pommier, P. Lanciano, C. Schaefer, P. Arnoux, G. Giordano, B. Guigliarelli, A. Magalon.
Cardiolipin-based respiratory complex activation in bacteria.
Proceedings of the National Academy of Sciences, 2011, 108 (19), 7781 – 7786. DOI: 10.1073/pnas.1010427108 HAL: hal-00677353v1

Sonia Longhi, Valérie Belle, André Fournel, Bruno Guigliarelli, Frédéric Carrière.
Probing structural transitions in both structured and disordered proteins using site-directed spin-labeling EPR spectroscopy.
J. Peptide Sci., 2011, 17 (5), 315 – 328. DOI: 10.1002/psc.1344

Carole Baffert, Luca Bertini, Thomas Lautier, Claudio Greco, Kateryna Sybirna, Pierre Ezanno, Emilien Etienne, Philippe Soucaille, Patrick Bertrand, Hervé Bottin, Isabelle Meynial-Salles, Luca De Gioia, Christophe Léger.
CO Disrupts the Reduced H-Cluster of FeFe Hydrogenase. A Combined DFT and Protein Film Voltammetry Study.
J. Am. Chem. Soc., 2011, 133 (7), 2096 – 2099. DOI: 10.1021/ja110627b HAL: hal-02652182v1

Pierre-Pol Liebgott, Antonio L. de Lacey, Bénédicte Burlat, Laurent Cournac, Pierre Richaud, Myriam Brugna, Victor M. Fernandez, Bruno Guigliarelli, Marc Rousset, Christophe Léger, Sébastien Dementin.
Original Design of an Oxygen-Tolerant [NiFe] Hydrogenase: Major Effect of a Valine-to-Cysteine Mutation near the Active Site.
J. Am. Chem. Soc., 2011, 133 (4), 986 – 997. DOI: 10.1021/ja108787s HAL: hal-01977599v1


Fanny Leroux, Pierre-Pol Liebgott, Laurent Cournac, Pierre Richaud, Arlette Kpebe, Bénedicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Christophe Léger, Marc Rousset.
Is engineering O2-tolerant hydrogenases just a matter of reproducing the active sites of the naturally occurring O2-resistant enzymes?.
International Journal of Hydrogen Energy, 2010, 35 (19), 10770 – 10777. DOI: 10.1016/j.ijhydene.2010.02.071 HAL: hal-01977635v1

Rodrigo Arias-Cartin, Sevdalina Lyubenova, Pierre Ceccaldi, Thomas Prisner, Axel Magalon, Bruno Guigliarelli, Stéphane Grimaldi.
HYSCORE Evidence That Endogenous Mena- and Ubisemiquinone Bind at the Same Q Site (QD) of Escherichia coli Nitrate Reductase A.
J. Am. Chem. Soc., 2010, 132 (17), 5942 – 5943. DOI: 10.1021/ja1009234 HAL: hal-00677673v1

Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Émilien Étienne, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Dependence of Catalytic Activity on Driving Force in Solution Assays and Protein Film Voltammetry: Insights from the Comparison of Nitrate Reductase Mutants.
Biochemistry, 2010, 49 (11), 2424 – 2432. DOI: 10.1021/bi902140e HAL: hal-00677461

Pierre-Pol Liebgott, Fanny Leroux, Bénédicte Burlat, Sébastien Dementin, Carole Baffert, Thomas Lautier, Vincent Fourmond, Pierre Ceccaldi, Christine Cavazza, Isabelle Meynial-Salles, Philippe Soucaille, Juan Carlos Fontecilla-Camps, Bruno Guigliarelli, P.
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nat Chem Biol, 2010, 6, 63 – 70. DOI: 10.1038/nchembio.276 HAL: hal-01977629


Stéphane Grimaldi, Rodrigo Arias-Cartin, Pascal Lanciano, Sevdalina Lyubenova, Burkhard Endeward, Thomas F. Prisner, Axel Magalon, Bruno Guigliarelli.
Direct Evidence for Nitrogen Ligation to the High Stability Semiquinone Intermediate in Escherichia coli Nitrate Reductase A.
J. Biol. Chem., 2009, 285 (1), 179 – 187. DOI: 10.1074/jbc.M109.060251 HAL: hal-00677704v1

Sébastien Dementin, Fanny Leroux, Laurent Cournac, Antonio L. de Lacey, Anne Volbeda, Christophe Léger, Bénédicte Burlat, Nicolas Martinez, Stéphanie Champ, Lydie Martin, Oliver Sanganas, Michael Haumann, Víctor M. Fernández, Bruno Guigliarelli, Ju.
Introduction of Methionines in the Gas Channel Makes [NiFe] Hydrogenase Aero-Tolerant.
J. Am. Chem. Soc., 2009, 131 (29), 10156 – 10164. DOI: 10.1021/ja9018258

Jenny Erales, Magali Lorenzi, Régine Lebrun, André Fournel, Emilien Etienne, Carine Courcelle, Bruno Guigliarelli, Brigitte Gontero, Valérie Belle.
A New Function of GAPDH from Chlamydomonas reinhardtii: A Thiol−Disulfide Exchange Reaction with CP12.
Biochemistry, 2009, 48 (25), 6034 – 6040. DOI: 10.1021/bi900569h HAL: hal-00429826


Vincent Fourmond, Bénédicte Burlat, Sébastien Dementin, Pascal Arnoux, Monique Sabaty, Séverine Boiry, Bruno Guigliarelli, Patrick Bertrand, David Pignol, Christophe Léger.
Major Mo(V) EPR Signature of Rhodobacter sphaeroides Periplasmic Nitrate Reductase Arising from a Dead-End Species That Activates upon Reduction. Relation to Other Molybdoenzymes from the DMSO Reductase Family.
J. Phys. Chem. B, 2008, 112 (48), 15478 – 15486. DOI: 10.1021/jp807092y HAL: hal-00336002

F. Leroux, S. Dementin, B. Burlat, L. Cournac, A. Volbeda, S. Champ, L. Martin, B. Guigliarelli, P. Bertrand, J. Fontecilla-Camps, M. Rousset, C. Leger.
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences, 2008, 105 (32), 11188 – 11193. DOI: 10.1073/pnas.0803689105 HAL: hal-00336010v1

Valérie Belle, Sabrina Rouger, Stéphanie Costanzo, Elodie Liquière, Janez Strancar, Bruno Guigliarelli, André Fournel, Sonia Longhi.
Mapping α-helical induced folding within the intrinsically disordered C-terminal domain of the measles virus nucleoprotein by site-directed spin-labeling EPR spectroscopy.
Proteins, 2008, 73 (4), 973 – 988. DOI: 10.1002/prot.22125

Carole Baffert, Marie Demuez, Laurent Cournac, Bénédicte Burlat, Bruno Guigliarelli, Patrick Bertrand, Laurence Girbal, Christophe Léger.
Hydrogen-Activating Enzymes: Activity Does Not Correlate with Oxygen Sensitivity.
Angew. Chem. Int. Ed., 2008, 47 (11), 2052 – 2054. DOI: 10.1002/anie.200704313 HAL: hal-00336018


Pascal Lanciano, Adrien Savoyant, Stéphane Grimaldi, Axel Magalon, Bruno Guigliarelli, Patrick Bertrand.
New Method for the Spin Quantitation of [4Fe−4S]+ Clusters with S = 3 / 2. Application to the FS0 Center of the NarGHI Nitrate Reductase from Escherichia coli.
J. Phys. Chem. B, 2007, 111 (48), 13632 – 13637. DOI: 10.1021/jp075243t HAL: hal-00336023v1

Sébastien Dementin, Pascal Arnoux, Bettina Frangioni, Sandrine Grosse, Christophe Léger, Bénédicte Burlat, Bruno Guigliarelli, Monique Sabaty, David Pignol.
Access to the Active Site of Periplasmic Nitrate Reductase:  Insights from Site-Directed Mutagenesis and Zinc Inhibition Studies.
Biochemistry, 2007, 46 (34), 9713 – 9721. DOI: 10.1021/bi700928m HAL: hal-00336040

Patrick Bertrand, Bettina Frangioni, Sébastien Dementin, Monique Sabaty, Pascal Arnoux, Bruno Guigliarelli, David Pignol, Christophe Léger.
Effects of Slow Substrate Binding and Release in Redox Enzymes:  Theory and Application to Periplasmic Nitrate Reductase.
J. Phys. Chem. B, 2007, 111 (34), 10300 – 10311. DOI: 10.1021/jp074340j HAL: hal-00336030

Eric Pilet, Wolfgang Nitschke, Ursula Liebl, Marten H. Vos.
Accommodation of NO in the active site of mammalian and bacterial cytochrome c oxidase aa3.
Biochimica et Biophysica Acta (BBA) – Bioenergetics, 2007, 1767 (5), 387 – 392. DOI: 10.1016/j.bbabio.2007.03.001 HAL: hal-00324353v1

Pascal Lanciano, Axel Magalon, Patrick Bertrand, Bruno Guigliarelli, Stéphane Grimaldi.
High-Stability Semiquinone Intermediate in Nitrate Reductase A (NarGHI) from Escherichia coli Is Located in a Quinol Oxidation Site Close to Heme bD.
Biochemistry, 2007, 46 (18), 5323 – 5329. DOI: 10.1021/bi700074y HAL: hal-00336035v1

Pascal Lanciano, Alexandra Vergnes, Stéphane Grimaldi, Bruno Guigliarelli, Axel Magalon.
Biogenesis of a Respiratory Complex Is Orchestrated by a Single Accessory Protein.
J. Biol. Chem., 2007, 282 (24), 17468 – 17474. DOI: 10.1074/jbc.M700994200 HAL: hal-03549606v1


M. Gabriela Almeida, Célia M. Silveira, Bruno Guigliarelli, Patrick Bertrand, José J.G. Moura, Isabel Moura, Christophe Léger.
A needle in a haystack: The active site of the membrane-bound complex cytochrome c nitrite reductase.
2006, 581 (2), 284 – 288. DOI: 10.1016/j.febslet.2006.12.023 HAL: hal-00336044

Gérard Lipowski, Ursula Liebl, Bruno Guigliarelli, Wolfgang Nitschke, Barbara Schoepp-Cothenet.
Conformation of the c 552 : aa 3 electron transfer complex in Paracoccus denitrificans studied by EPR on oriented samples.
2006, 580 (25), 5988 – 5992. DOI: 10.1016/j.febslet.2006.09.038 HAL: hal-00824462v1

Hyperthermostable and oxygen resistant hydrogenases from a hyperthermophilic bacterium Aquifex aeolicus: Physicochemical properties.
International Journal of Hydrogen Energy, 2006, 31 (11), 1424 – 1431. DOI: 10.1016/j.ijhydene.2006.06.007 HAL: hal-00335435

Sébastien Dementin, Valérie Belle, Patrick Bertrand, Bruno Guigliarelli, Géraldine Adryanczyk-Perrier, Antonio L. De Lacey, Victor M. Fernandez, Marc Rousset, Christophe Léger.
Changing the Ligation of the Distal [4Fe4S] Cluster in NiFe Hydrogenase Impairs Inter- and Intramolecular Electron Transfers.
J. Am. Chem. Soc., 2006, 128 (15), 5209 – 5218. DOI: 10.1021/ja060233b HAL: hal-00335157v1

Christophe Léger, Florence Lederer, Bruno Guigliarelli, Patrick Bertrand.
Electron Flow in Multicenter Enzymes:  Theory, Applications, and Consequences on the Natural Design of Redox Chains.
J. Am. Chem. Soc., 2006, 128 (1), 180 – 187. DOI: 10.1021/ja055275z HAL: hal-00336055v1


Claude More, Marcel Asso, Guy Roger, Bruno Guigliarelli, Jorge Caldeira, José Moura, Patrick Bertrand.
Study of the Spin−Spin Interactions between the Metal Centers of Desulfovibrio gigas Aldehyde Oxidoreductase:  Identification of the Reducible Sites of the [2Fe-2S] 1+,2+ Clusters.
Biochemistry, 2005, 44 (34), 11628 – 11635. DOI: 10.1021/bi0510025 HAL: hal-00336065v1

Marianne Guiral, Gisèle Leroy, Pierre Bianco, Philippe Gallice, Bruno Guigliarelli, Mireille Bruschi, Wolfgang Nitschke, Marie-Thérèse Giudici-Orticoni.
Interaction and electron transfer between the high molecular weight cytochrome and cytochrome c3 from Desulfovibrio vulgaris Hildenborough: Kinetic, microcalorimetric, EPR and electrochemical studies.
Biochimica et Biophysica Acta (BBA) – General Subjects, 2005, 1723 (1-3), 45 – 54. DOI: 10.1016/j.bbagen.2005.01.015 HAL: hal-00339978v1

Stéphane Grimaldi, Pascal Lanciano, Patrick Bertrand, Francis Blasco, Bruno Guigliarelli.
Evidence for an EPR-Detectable Semiquinone Intermediate Stabilized in the Membrane-Bound Subunit NarI of Nitrate Reductase A (NarGHI) from Escherichia coli.
Biochemistry, 2005, 44 (4), 1300 – 1308. DOI: 10.1021/bi048009r HAL: hal-03549620v1


Eric Pilet, Wolfgang Nitschke, Fabrice Rappaport, Tewfik Soulimane, Jean-Christophe Lambry, Ursula Liebl, Marten H. Vos.
NO Binding and Dynamics in Reduced Heme−Copper Oxidases aa 3 from Paracoccus denitrificans and ba 3 from Thermus thermophilus.
Biochemistry, 2004, 43 (44), 14118 – 14127. DOI: 10.1021/bi0488808 HAL: hal-00836426v1

Christophe Léger, Sébastien Dementin, Patrick Bertrand, Marc Rousset, Bruno Guigliarelli.
Inhibition and Aerobic Inactivation Kinetics of Desulfovibrio fructosovorans NiFe Hydrogenase Studied by Protein Film Voltammetry.
J. Am. Chem. Soc., 2004, 126 (38), 12162 – 12172. DOI: 10.1021/ja046548d HAL: hal-00336069

Bettina Frangioni, Pascal Arnoux, Monique Sabaty, David Pignol, Patrick Bertrand, Bruno Guigliarelli, Christophe Léger.
In Rhodobacter sphaeroides Respiratory Nitrate Reductase, the Kinetics of Substrate Binding Favors Intramolecular Electron Transfer.
J. Am. Chem. Soc., 2004, 126 (5), 1328 – 1329. DOI: 10.1021/ja0384072 HAL: hal-00338609v1


Marianne Brugna-Guiral, Pascale Tron, Wolfgang Nitschke, Karl-Otto Stetter, Benedicte Burlat, Bruno Guigliarelli, Mireille Bruschi, Marie Thérèse Giudici-Orticoni.
[NiFe] hydrogenases from the hyperthermophilic bacterium Aquifex aeolicus: properties, function, and phylogenetics.
Extremophiles, 2003, 139 (2), 139 – 157. DOI: 10.1007/s00792-002-0306-3 HAL: hal-00343693v1


Myriam Brugna, Wolfgang Nitschke, Marcel Asso, Bruno Guigliarelli, Danielle Lemesle-Meunier, Christian Schmidt.
Redox Components of Cytochrome bc -type Enzymes in Acidophilic Prokaryotes.
J. Biol. Chem., 1999, 274 (24), 16766 – 16772. DOI: 10.1074/jbc.274.24.16766


Mireille Bruschi, Mireille Woudstra, Bruno Guigliarelli, Marcel Asso, Elisabeth Lojou, Yves Petillot, Chantal Abergel.
Biochemical and Spectroscopic Characterization of Two New Cytochromes Isolated from Desulfuromonas acetoxidans.
Biochemistry, 1997, 36 (35), 10601 – 10608. DOI: 10.1021/bi9707741